Objective To observe the effects of paclitaxel,mitomycin,5-FU and cisplatin on the proliferation of tongue carcinoma cells and to guide the reasonable using of chemotherapeutic drugs and avoids the side-effects.Methods The fresh tongue carcinoma tissues from surgery were collected and unicellular suspension were prepared.Then paclitaxel,mitomycin,5-FU and cisplatin were used to investigate the drug sensitivity on primary tongue carcinoma cells and a blank control group was set up.The inhibitory effects of chemotherapeutic drugs on the growth of tongue carcinoma cells and the cell cycle phases were observed by MTT colorimetry and flow cytometry.Results The inhibitory rates of paclitaxel,mitomycin and 5-FU on tongue carcinoma cell proliferation were 45.3%,37.3%,and 36.2%,respectively,and in control group it was 2.1%,the difference was significant(P

Objective:To investigate the expression of P33~(ING1) protein in oral squamous cell carcinoma(OSCC) and its clinical significance. Methods:All samples of normal and cancer tissues from 30 patients with OSCC were examined for protein expression of P33~(ING1) by SP immunohistochemical staining.Results:The positive rate of P33ING1 in OSCC was 40%, that in normal oral mucosa tissue 100% (P

This article is to review the basic principles of renal perfusion in CT and MR,imaging method and perfusion characteristics in kidney diseases.It shows that in addition to the morphologic information,perfusion imaging can also provide functional and metabolic information about the renal neoplasms,which is very helpful for not only the differential diagnosis,but also the evaluation of tumor biological behaviors,it will have a bright future in clinical application.

Objective: To evaluate the electron beam computed tomography(EBCT) three-dimensional reconstruction in the diagnosis of bowel obstruction.Methods: Seventy-eight patients suspected of bowel obstruction underwent EBCT with two-phase enhancement scanning and 32 of them were re-established by corona-MPR and sagittal-MPR.All the EBCT results were analyzed and compared with the clinical findings.Results: Seventy-three of the cases were diagnosed as bowel obstruction by EBCT,which were all confirmed by surgery or follow-up,with an accuracy rate of 100% for location diagnosis and 86.3% for etiology.Conclusion: EBCT 3D-reconstruction has great advantages in determining the location,etiology and blood-supply of bowel obstruction.

Objective To investigate the in vitro effect of multi glycosidorum triptery (MGT) on cytokine production by splenocytes of oxazolone (OXZ) induced colitis in murine model. Methods Six mg of OXZ (in 50% of ethanol) was administered in male SJL/J mice intrarectally to induce colitis and mice were sacrificed 3 days later. Isolated splenocytes were cultured for 24 hours in the presence of PMA and ionomycin, MGT of 0.1 mg/ml or 0.01 mg/ml was added to the culture medium of splenocytes. Production of IFN ? and interleukin 4 (IL 4) in the supernatant was measured by ELISA. Results The production of IFN ? was suppressed by both 0.01 mg/ml and 0.1 mg/ml of MGT [normal control ( 1.24 ? 0.13 ) pg/ml→(0.97?0.26) pg/ml→(0.87?0.18) pg/ml, P

Objective:To develop bifunctional anti-human lingual cancer/anti-VEGF antibody.Methods:Antigen of lingual cancer was prepared from Tca 8113 cells and that of VEGF from endothelial cells of umbilical cord vein.The hybridoma cells of anti-human ligual cancer/anti-VEGF were prepared by the technique of secondary hybridoma.The antibody was identified by ELISA. Results:The specific conjugation rate between bifunctional antibody and lingual cancer tissue was 85.29%, and that between the antibody and vascular endothelial cells was 82.35%.Conclusion:The bifunctional antibody of anti-human lingual cancer/anti-VEGF has good targeting potential.

ObjectiveTo study the feasibility of analyzing photosensitizers absorbing process by cells based on image processing technology.MethodsFluorescence images of Laryngeal cells were taken by inverted fluorescence microscope after adding photosensitizers for a certain time.Parameter L that reflects the fluorescence intensity of cells in different times was obtained and the calculation results were compared.ResultsThe fluorescence intensity of cells increased over time.Sobel operater and Otsu algorithm can both reflect the fluorescence intensity of images.ConclusionImage processing technology can effectively analyzes the process of photosensitizers absorbed by cells.

Objective To investigate the inhibition effect of pituitary tumor transforming gene(PTTG) antisense oligodeoxynucleotide(ASODN) on C6 glioblastoma in rats.Methods The C6 glioma cells were injected into the right caudate nucleus.PTTG-ASODN of 8 or 16 ?g/ml was injected into the tumor-affected area with stereotactic technique immediately,at 1st and 2nd week after inoculation of C6 cells.Three weeks after C6 cell inoculation,all rats were killed and the tumors were excised,then tumor volume was calculated and pathologically analysed,and immunohistochemical statining for GFAP, PCNA ang PTTG was performed.Results PTTG-ASODN could suppress the proliferation of C6 glioblastoma in a dose-and time-dependent manner.The inhibition effect was better when large-dose PTTG-ASODN was repeatedly used for glioblastoma as early as possible.Conclusion PTTG-ASODN can suppress the proliferation of glioblastoma,which may become a new strategy of gene therapy for glioblastoma.

BACKGROUND: Generally speaking, neuroendocrine cells have been not observed in normal breast tissue but found in breast carcinoma tissue which was affected by local microenvironment and hormone level during differentiation of breast epithelial stem cells.OBJECTIVE: By detecting expressions of breast cancer resistance protein (BCRP), cytokeratin-8 (CK8), and chromogranin-A (CgA) in breast carcinoma tissue, to explore the possible mechanism of neuroendocrine cells observed in breast carcinoma tissue during differentiation of multi-lineage potential of bone marrow mesenchymal stem cells.METHODS: BCRP, CK8, and CgA were used as markers for SP stem cells, glandular epithelium differentiation, and neuroendocrine differentiation, respectively. Immunohistochemistry was used to detect the expressions of BCRP, CK8, and CgA in breast tissues of 89 subjects and analyze their correlation. RESULTS AND CONCLUSION: Both BCRP and CK8 expressions were observed in normal breast tissues, hyperplastic tissues, and breast carcinoma tissue. BCRP expression was increased in the breast carcinoma tissue; CK8 expression was decreased with the abnormal differentiation of breast tissue; CgA expression was only detected in breast carcinoma tissue. BCRP expression was significantly correlated with positive CgA expression (P < 0.01), but it was no correlation with positive CK8 expression in normal breast tissues, hyperplastic tissues, and breast carcinoma tissue (P=0.069). The results suggested that neuroendocrine cells were not observed in both normal breast tissues and hyperplastic tissues but in breast carcinoma tissue, which possibly correlated to differentiation of multi-lineage potential of bone marrow mesenchymal stem cells.

AimTo establish the model of adriamycin- induced nephritic syndrome in rats and its pathological change wasobserved. MethodsThe model of nephritic syndrome in rats was established by intravenous injection of adriamycin in the dose of 5mg · kg-1 and 3 mg · kg-1 respectively at the first day and 15 th day. The amount of urine protein was measured at the 1、2、3、4、6、8 and 12 th week and the serum biochemical pa rameters of TG, TCH, BUN, Cr, TP and ALB were detected at the 6 th and 12 th week. Results The urine protein and the serum biochemic parameters were increased significantly, except the TP and ALB, which were decreased. Pathological changes at the sixth week was similar to minimal changes in glomerulonephritis, and at the 12 th week, sclerosis of glomeruli of kidney could be found. ConclusionAfter first injection,the changes of kidney were like those inminimal change glomerulonephritis of human being. But after the second injection. The kidney had the changes of focal segmental glomeralosclerosis.