2.Expression of Maspin in invasive fungal rhinosinusitis
Hewei YU ; Zonghui KANG ; Dan PAN ; Siwen XIA ; Xianhui HU ; Yingwu CHEN ; Yunsheng HE ; Yideng HUANG
Chinese Archives of Otolaryngology-Head and Neck Surgery 2016;23(6):349-352
OBJECTIVE To explore the expression of Maspin in invasive fungal rhinosinusitis (IFRS) and the value of Maspin in the diagnosis of IFRS. METHODS Forty two cases of fungal rhinosinusitis (FRS) were set as the experimental group, which included 12 cases of IFRS and 30 cases of noninvasive fungal rhino-sinusitis (NIFRS). At the same time, 30 cases of chronic rhino-sinusitis were set as control group. Immunohistochemistry (IHC) was used to detect the expression of Maspin. RESULTS Compared with the control group, the expression of Maspin in FRS group decreased statistically (t=-3.367, P<0.05). The IFRS group, compared with other two groups, had the lowest expression of Maspin (t=-3.390, P<0.05; t=-4.143, P<0.05). By using Maspin score of 5.70 as the cut-off point, the sensitivity and specificity for the diagnosis of IFRS was 91.7% and 88.3% respectively. CONCLUSION The expression of Maspin is very low in IFRS group. Down-regulation of Maspin expression may be a potential indicator for diagnosis of IFRS.
3.Role of Ultrasound Elastography in Evaluation of Lymphedema and Rehabilitation for It (review)
Lin YUAN ; Sufang SHEN ; Yue LI ; Jing WU ; Yingwu CHEN ; Liping HUANG
Chinese Journal of Rehabilitation Theory and Practice 2017;23(9):1056-1059
Elastography can study the soft tissues by quantitative imaging of strain and elastic modulus distributions in soft tissues. For lymphedema, the strain profile of the soft tissues can be observed with the elastography to classify the lymphedema and evaluate the effect of rehabilitation.
4.Regulation of key enzymes in tryptophan biosynthesis pathway in Escherichia coli.
Jinlong YU ; Jing WANG ; Jianxin LI ; Changjiang GUO ; Yingwu HUANG ; Qishou XU
Chinese Journal of Biotechnology 2008;24(5):844-850
To improve tryptophan production in Escherichia coli, key genes in the tryptophan biosynthesis pathway -aroG, trpED, trpR and tnaA were manipulated. TrpR gene was knocked out to eliminate the repression on the key genes controlling tryptophan biosynthesis and transportation on bacteria chromosome, and the tryptophan degradation was blocked by tnaA gene knockout. Then the bottleneck in tryptophan biosynthesis pathway was removed by co-expressing aroGfbr gene and trpEDfbr gene. Compared with the MG1655, the tryptophan production of trpR knockout and double-genes knockout strains was improved 10-folds and about 20-folds, respectively. After the trpEDfbr was expressed, the tryptophan production increased to 168 mg/L, and when the aroGfbr and trpEDfbr were co-expressed, the tryptophan production increased to 820 mg/L. This work laid the foundation for further construction of higher-efficient engineered strain for tryptophan production.
3-Deoxy-7-Phosphoheptulonate Synthase
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metabolism
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Amino Acid Transport Systems
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genetics
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Bacterial Proteins
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genetics
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Cloning, Molecular
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Escherichia coli
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genetics
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metabolism
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Escherichia coli Proteins
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genetics
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Gene Knockout Techniques
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Genetic Engineering
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Repressor Proteins
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genetics
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Tryptophan
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biosynthesis
5.Comparisons of perioperative period features and forward outcome between extracorporeal membrane oxygenation-conventional cardiopulmonary switch and off-pump high-risk coronary artery bypass grafting
Lei HUANG ; Tong LI ; Xiaomin HU ; Dawei DUAN ; Yingwu LIU ; Peng WU ; Qiang ZHANG ; Yuheng LANG ; Xin LU ; Song WANG ; Yue ZHAO
Chinese Journal of Geriatrics 2017;36(4):422-427
Objective To compare the perioperative characteristics and long term outcomes between extracorporeal membrane oxygenation (ECMO)-conventional cardiopulmonary switch (experimental group,26 cases) and off-pump high-risk coronary artery bypass grafting (OPCABG group,24cases).Methods Perioperative characteristics and survival rate were retrospectively analyzed between experimental group and OPCABG group.Long term survival rates without major cardiovascular adverse events (MACE) were comparatively analyzed via Kaplan-Meier curves.Results The average Euroscore value were 11.7 ± 2.4 and 10.9 ± 2.0,respectively(P =0.208).The experimental group had a higher complete revascularization rate (96.2% vs.66.7%,P =0.009),a shorter length of postoperative ECMO support [(33.1±23.6)h vs.(80.8±18.5)h],an intensive care unit stay[(4.8±1.1)d vs.(10.2±9.0)d]and a hospital stay [(17.7±6.3)d vs.(28.2±17.5)d] (all P<0.05) as compared with OPCABG group.Preoperative New York Heart Association (NYHA) grading of cardiac function (r =0.511,P =0.008) and intraoperative ultrafiltration volume (r =-0.442,P =0.024) were significantly correlated with postoperative ECMO continuation in the experimental group.The follow-up period was (45.4 ± 15.2) months.The experimental group had a higher survival rate without MACE than had the OPCABG group (Log-rank test:x2=4.828,P=0.028).Conclusions The ECMO-conventional cardiopulmonary switch mode might facilitate a higher complete revascularization,a lower incidence of postoperative morbidities and improve the longterm survival rate without MACE for patients with high risks.
6. Effect of the ischemic post-conditioning on the prevention of the cardio-renal damage in patients with acute ST-segment elevation myocardial infarction after primary percutaneous coronary intervention
Yunyun WANG ; Tong LI ; Yingwu LIU ; Bojiang LIU ; Xiaomin HU ; Yu WANG ; Wenqing GAO ; Peng WU ; Lei HUANG ; Xin LI ; Wenjin PENG ; Meng NING
Chinese Journal of Cardiology 2017;45(4):277-282
Objective:
To evaluate the effect of the ischemic post-conditioning (IPC) on the prevention of the cardio-renal damage in patients with acute ST-segment elevation myocardial infarction (STEMI) after primary percutaneous coronary intervention (PPCI).
Methods:
A total of 251 consecutive STEMI patients underwent PPCI in the heart center of Tianjin Third Central Hospital from January 2012 to June 2014 were enrolled in this prospective, randomized, control, single-blinded, clinical registry study. Patients were randomly divided into IPC group (123 cases) and control group (128 cases) with random number table. Patients in IPC group underwent three times of inflation/deflation with low inflation pressure using a balloon catheter within one minute after culprit vessel blood recovery, and then treated by PPCI. Patients in control group received PPCI procedure directly. The basic clinical characteristics, incidence of reperfusion arrhythmia during the procedure, the rate of electrocardiogram ST-segment decline, peak value of myocardial necrosis markers, incidence of contrast induced acute kidney injury(CI-AKI), and one-year major adverse cardiovascular events(MACE) which including myocardial infarction again, malignant arrhythmia, rehospitalization for heart failure, repeat revascularization, stroke, and death after the procedure were analyzed between the two groups.
Results:
The age of IPC group and control group were comparable((61.2±12.6) vs. (64.2±12.1) years old,