Objective: To investigate the effect of lignan 1 on the proliferation and apoptosis of liver cancer HepG2 cells. Methods: The proliferation of HepG2 cells after treatment with different concentrations of lignan 1 was detected by MTT assay; the cell cycle distribution and the apoptosis were examined by FCM; the expression level of cleaved caspase 3 protein was determined by using Western blotting. Results: The proliferation of HepG2 cells after treatment with 1.25-20 μg/mL lignan 1 for 24, 48 and 72 h was inhibited in a time- and dose-dependent manner (all P < 0.05). The percentage of HepG2 cells in G2/M phase and the apoptotic rate of HepG2 cells after treatment with 2.5-20 μg/mL lignan 1 were increased (all P < 0.05). The expression level of cleaved caspase 3 protein was up-regulated (P < 0.05). Conclusion: Lignan 1 can inhibit the proliferation of liver cancer HepG2 cells and induce the apoptosis. This effect may be related to up-regulating the expression level of cleaved caspase 3 protein.

Objective:To study the efficacy of laparoscopic ovarian cyst aspiration in the treatment of neonatal simple ovarian cyst.Methods:From August 2019 to December 2021, infants with neonatal simple ovarian cyst receiving laparoscopic ovarian cyst aspiration in the Department of Pediatrics of Gansu Provincial Maternity and Child-care Hospital were retrospectively studied. The clinical characteristics, age of surgery, operation duration, length of hospital stay, complications and follow-up were analyzed.Results:A total of 6 full-term infants were included. Simple ovarian cysts were located on the right side of the body in 5 cases and on the left in 1 case. The average cyst diameter was (6.1±1.4) cm, the surgery were performed at 2~5 d of age, the average duration of the surgery was (18.8±2.4) min and the average hospital stay was (5.3±1.0) d. No complications occurred before or after surgery. All the 6 infants had favorable growth and development. The ovarian cysts were all enlarged again in 1 month after surgery, then gradually shrunk at 3 to 6 months after surgery and completely resolved in 2 cases.Conclusions:Neonatal simple ovarian cysts are more common on the right side of the body and laparoscopic ovarian cyst aspiration has good and safe clinical efficacy.