0.05). Conclusion:Certain concentration of TG can inhibit the apoptosis of Schwann’s cell and the expression of major histocompatibility antigen of cold preserved sciatic nerve in rats,and decrease rejection after nerve allograft.

Objective To study MR imaging features of bone marrow after priming.Methods 11 cases with lymphoma or myeloma alleviated after treatment were administration of recombinant human granulocyte colony-stimulating factor(rhG-CSF)injection.Two regions of bone marrow in L3 and iliac were followed up with T1WI,and STIR fat saturation before treatment(0 week),and 1 week,2 weeks and 4 weeks after treatment respectively.MRI features were observed by three experienced doctors independently and the average results acted as the final result.In the meantime,CNR of marrow and fat were calculated and paired sample test.Statistical process was performed in SPSS 10.0 for Windows.Results In comparison with 0 week,the signal intensity of bone marrow was no changed in all cases one week after intensive chemotherapy,slight high in 5 cases 2 weeks later and obvious low signal intensity in all cases three weeks after priming with rhG-CSF.In comparison with 0 week,CNR was no any different significance at 1 week,2 weeks and 4 weeks.Bone marrow changes in lumber and iliac were parallel except one case at 2 weeks and one case at 4 weeks.Conclusion Obvious low signal intensity of bone marrow is found three weeks after priming with rhG-CSF.

0.05). Homogenous or inhomogenous high signal in aplastic anemia were detected with decreased T 1 value. Homogenous iso-intensity was revealed in acute pure red cell aplasia with normal T 1 value. Aplastic anemia and acute pure red cell aplasia had a homogenous signal on T 2WI, their T 1 value showed significant difference (P

Objective To evaluate the MRI in the diagnosis of aplastic anemia (AA) and MRI changes after therapy. Methods MR imaging of lumber spine in 16 patients with AA proved pathologically were performed on 0.5 T superconductive MR unit with T 1WI, T 2WI, and STIR, slice thick 5 mm, slice gap 1 mm, matrix 180?256. Thirty cases of normal volunteers were examined as control group. Lumber spines of the two groups were studied by observation of MRI findings, measurement of the T 1 relaxation value. In AA group, the percentage of the hypointense signal areas in the centrum was calculated. Results Three patterns were classified by MR appearance in lumber spine of our cases. MR findings of pattern 1 (2 cases) were hyperintense on T 1WI, isointense on T 2WI, and hypointense on STIR, and all two cases were acute aplastic anemia (AAA). In pattern 2 (7 cases), a few irregular hypointense signals appeared in the background of hyperintense signal on all 3 sequences, and 5 of them were AAA. Pattern 3 (7 cases) demonstrated mixed irregular shape of hyperintense and hypointense signals, all 7 cases were chronic aplastic anemia (CAA). The percentage of the area of hypointense signal in AAA was significantly smaller than that in CAA (t=30 00, P

Objective To evaluate the diagnostic value of MRI and X ray and to analyze MRI and X ray characteristics in Med anemia Methods MRI was performed in thoracic spine,lumbar spine, sacral spine,pelvis,and superior femurs in 15 patients with pathologically proved Med anemia with T 1WI and T 2WI Seven of them were examined on X ray Twenty volunteers were examined on MR as control group The authors analyzed the manifestations of MRI in the Med anemia, including abnormality of signal intensity of bone marrow,T 1 value,vertebral alteration of size and shaped, spinal cord compression due to epidural extramedullary haematopoiesis, and compared with the findings of bone on X ray Results In the control group, bone marrow showed an inhomogeneous iso signal intensity with band shaped, triangular or motley high signal intensity in the middle or back part of the vertebra, neck of the femur on T 1WI and a homogeneous iso signal intensity on T 2WI in the vertebra, pelvis, and superior femur All cases with Med anemia had low homogenous signal intensity in the bone marrow of the vertebra, pelvis and superior femur on T 1WI but had no alterations on T 2WI The spinal cord was constricted resulting from protruding crushed vertebra with bullet like shape in 4 cases The soft tissue masses, confirmed as extramedullary haematopoiesis by operation, were demonstrated in 4 cases Among 7 cases with X ray examinations, 2 cases showed normal on X ray but abnormal bone marrow on MRI, another 5 cases demonstrated bone alteration including osteoporosis, widening of striations, enlargement of ribs and vertebras T 1 value showed (897 4?75 43)ms in the Med anemia group and (401 5?28 1)ms in the control group with significant statistical difference ( P

Objective To find the X-ray characteristic and diadynamic method in congenital defect of muscular layer in stomach wall. Methods Six cases confirmed by operation and pathology were collected,underwent eight standing abdomen plain films,underwent 5 decubitus abdomen plain films in 6 cases. Results The appearances in standing plain film of freeing gas under diaphragm, eventration of diaphragm, passing through the whole abdomen of gas and liquid plane, vanished gastriole were found in 6 cases. Superior border of liver was found in 4 cases.The appearances in decubitus abdomen plain films of abdominal distension, increasing gas gathered in umbilical region, vanshed fat line in abdominal wall were found in 4 cases. Conclusion Large part of typical cases could be diagnosis by x-ray and clinical appearance.

OBJECTIVE:To observe clinical efficacy and safety of Bushen sanhan tongluo decoction combined with moxi-bustion and celecoxib in the treatment of knee osteoarthritis (KOA). METHODS:A total of 70 KOA patients were selected from Chongqing Kanghua Hospital during May 2014-Dec. 2015,and then divided into observation group and control group ac-cording to odd and even number,with 35 cases in each group. Control group was given Celecoxib capsule 0.2 g,qd;observa-tion group was additionally given Bushen sanhan tongluo decoction(one dose a day,300 mL,decocted with water,taking it 3 times in the morning,noon and night)and moxibustion. A treatment course lasted for 4 weeks,and both received 2 courses of treatment. Clinical efficacies as well as TCM syndrome score,VAS score,WOMAC score,lab indexes,joint condition be-fore and after treatment,the occurrence of ADR were compared between 2 groups. RESULTS:Total response rate of observa-tion group (85.71％) was significantly higher than control group (68.57％),with statistical significance (P<0.05). Before treatment,there was no statistical significance in above indexes between 2 groups (P>0.05). After treatment,TCM syn-drome score,VAS score,WOMAC score,erythrocyte sedimentation rate,CRP level and knee swelling score of 2 groups were decreased significantly,compared to before treatment;those indexes of observation group were significantly lower than those of control group,with statistical significance(P<0.05). There was no statistical significance in the number of bone fric-ative joint between 2 groups before and after treatment (P>0.05). There was no statistical significance in the incidence of ADR (5.71％ vs. 2.86％) between 2 groups (P>0.05). CONCLUSIONS:Bushen sanhan tongluo decoction combined with moxibustion and celecoxib can improve clinical symptoms,relieve joint pain,joint inflammation and swelling of KOA pa-tients with good safety.

Objective To evaluate MRI findings and X ray appearance in primary myelofibrosis (PMF) in marrow of lumber vertebra and pelvis. Methods Eleven cases of PMF with integrated clinical and pathology data were collected. All cases were examined with X ray and MRI simultaneously. The imaging findings of PMF were studied and compared with that of normal group. The MRI findings and X ray appearance of cases with PMF were evaluated and compared with each other to find their good quality, the shortcoming, and the relationships. Results X ray appearance in the plain film in cases with PMF: There were no abnormal findings in lumbar vertebra, iliac bone, and superior segment of femur in 5 of 11 cases. Blurred bone trabecula and ground glass changes in bone marrow were found in 3 of 11 cases. In another 3 cases, the bone trabeculas were obscured, tightly closed or mixed together, and the density of bone marrow were widespreadly high, and the difference in it couldn′t be distinguished. MRI findings in cases with PMF: MRI findings in different parts of lumbar vertebrae, iliac bone, and femur superior segments in all 11 cases were consistent. Compared with the control group, both images on T 1 and T 2 weighted spin echo displayed diffusely and well distributed lower signal intensity in 6 of 11 cases, but displayed scattered spot and patchy lowersignals in 5 of 11 cases in all parts of bone marrow including lumbar vertebra, iliac bone, and superior segment of femur. The extents of lower marrow signal intensity varied in different cases. The high signal in femoral head and greater trochanters remained unchanged as that of subcutaneous fat in 4 of 11 cases. The positive rate was 54.6%(6/11)in X ray findings, but 100%(11/11)in MRI appearances. Conclusion MRI has greater value than X ray in the early diagnosis of PMF. The early diagnostic value of X ray plain film for PMF is limited.

Objective To investigate MRI manifestations of lumbar and proximal femoral bone marrow changes before and after recombinant human granulocyte colony stimulating factor (rhG-CSF) was subcutaneous injected for healthy adults.Methods Twenty healthy blood stem cell donors without hematologic disease were enrolled in this study. All of them underwent lumbar sagittal and proximal femur coronal MRI examination with spin echo T1 WI and fat-suppressed T2WI.The first examination were performed before subcutaneous injection of rhG-CSF for comparison. In 4-7 days and 30-60 days after injection, the other two examinations were performed. The signal changes of lumbar and proximal femoral bone marrow were investigated by reading pictures and calculating the contrasted noise ratio (CNR).ResultsBefore rhG-CSF injection, all patients presented normal signal intensity of hone marrow. In 4-7 days after injection, all the 20 cases presented homogeneous signal decrease in lumbar vertebral bodys on T1 WI, accompanied by reduced fatty signal. In proximal femur, patchy or stripped hypointensity areas were found in intertrochanteric and subtrochanteric areas on T1 WI. On fat-suppressed T2 WI images, the signal of lumbar and proximal femoral bone marrow changed to equal or slightly-high signal intensity. In all cases,abnormal signal areas presented in lumbar and proximal femoral bone marrow occurred simultaneously in the same case.In the 10 cases received the third MRI during 30-60 days after rhG-CSF injection, signal intensity of lumbar bone marrow turned to normal in all sequence, but abnormal signal intensity areas were still existed and extended to distal part in femoral bone marrow, which appeared as symmetric stripped or patchy equal or slightly-low signal intensity on T1 WI and equal or slightly-high signal intensity on T2 WI. The CNR of lumbar bone marrow to subcutaneous fat before rhG-CSF injection, in 4-7 days and 30-60 days after rhG-CSF injection were 114. 11 ± 15. 11,71.04 ± 12. 25 and 91.64 ± 1 I. 68, respectively. Significant difference was found between before rhG-CSF injection and 4-7 days after injection ( P ＜ 0. 05 ) , but no significant difference between the others( P ＞ 0. 05 ). Conclusion After injection of rhG-CSF, the short-term changes of hematopoietic cells and fat content in bone marrow can be displayed on MRI, which provided non-invasive information for bone marrow transplantation.

Objective T9 provide candidate molecules for developing recombinant anti-idiotypic antibody (anti-Id) vaccine of gastric carcinoma by selection of recombinant anti-Id to monoclonal antibody ( McAb) MGb1 directed against the cancer with phage display technique.Methods Balb/c mice were immunized with MGb1 and the mRNA was isolated from the spleens of the immunized mice. The VL and VH cDNAs of the antibody were amplified separately by RT-PCR and assembled into ScFv DNAs with a linker DNA. The ScFv DNAs were ligated into the phagemid vector pCANTAB5E and the ligated sample was transformed into competent E. coli TG1. The transformed cells were infected with M13KO7 helper phage to yield recombinant phage antibody ScFv library. After four rounds of biopanning to the library with MGb1, the MGb1-positive clones were selected from the enriched phages by ELISA. The types of the anti-Id ScFv displayed on the selected phage clones were preliminarily identified by competition ELISA. Results The VL and VH cDNAs was about 320 bp and 340 bp, respectively. The ScFv DNA were about 750 bp. After four rounds panning to the phage antibody ScFv library with MGb1, 18 MGb1-positive phage clones displayed anti-Id ScFv were selected from 50 pre-selected phage clones, among which 4 clones displayed β or γ type anti-Id ScFv. Conclusion The phagedisplayed anti-Id ScFvs to McAb MGb1 are successfully selected by recombinant phage antibody technique, which might lay a foundation for screening the anti-Id ScFv possessing the characteristics of inducing anti-gastric carcinoma immunity.