Objective:To make a synthetic comment on treatment of children cerebral palsy with acupuncture in recent 10 years in China.Methods:Related papers were gotten from the journals between 1999-2008 in Chinese journal full-test database and VIP information-Chinese technology journal database by computer retrieval.The collected 143 papers were analyzed from the terms of clinical acupoints selection,treatment methods,time,and mechanism,and then summarized the clinical effects of acupuncture in treating children cerebral palsy.Results:The acupoints selection was focus on scalp acupuncture functional area and body points(including scalp points).Beside acupuncture treatment,the combination of acupoint embedding therapy,acupoint injection,and massage were also used.The therapy time was different from 20min to 4h,and the treatment course was usually be counted by month.The effects of acupuncture treatment were considered to be significant through improving brain circulation and recovering brain cells' function. Conclusion:Acupuncture treatment had advantages in improving the child'limbs function,nevertheless further studies on the research methods,study design and mechanism were all needed.

Objective To observe the correlation between mating days and pregnancy rate, the vaginal pessary number and the embryos number in female rats, for facilitating the whole planning of the relevant experiments. Methods 70 female Wistar rats were randomly mated with 20 male Wistar rats separately. Mating days and vaginal pessary were recorded. In late stage of the experiment, the number of pregnant rats and embryos were confirmed by post-mortem anatomy. Results Person correlation coefficient of the mating days and pregnancy rate in exhausting pessary rats was 0.83, P<0.05 (P=0.005); person correlation coefficient of the vaginal pessay number and the embryos was 0.16, P>0.05(P=0.18). Conclusion There was linearly dependent relationship between the mating days and pregnancy rate, the pregnancy rate of female rats become 100% when the mating days reached 4 days. But there was no such relationship between the vaginal pessary and the embryo number.

0.05), but insulin sensitivity was actually improved (P

AIM: To explore the effect of collagen and C-erbB-2 protein on the adhesion and the proliferation in hepatocellular carcinoma cells.METHODS: Hepatocellular carcinoma cell line(HepG-2) identified to positive for C-erbB-2 gene was used to study the adhesion and the growth feature by the action of rat tail collagen and C-erbB-2 antibody.RESULTS: The action of rat tail collagen to potentiated the adhesion in HepG-2 cells was significantly but no proliferation effect was observed. C-erbB-2 antibody inhibited the adhesion and proliferation of HepG-2 cells and also abolished the potentiated effect of rat tail collagen on the adhesion in HepG-2 cells.CONCLUSIONS: The signaling transduction mediated by C-erbB-2 protein was correlated to the adhesion and the proliferation of HepG-2. The blockage of C-erbB-2 gene signal transduction may be a strategic target to the treatment of liver cancer in the future.

AIM: To examine the effect of insulin on the proliferation of human hepatocellular carcinoma cell and its possible mechanisms. METHODS: The human hepatocellular carcinoma cell line(HepG-2) was used to study the changes in calcium ion across plasma membrane (Ca 2+ APM)under the action of insulin by the assay of atomic absorption spectrum, and in the proliferation under the action of insulin and calcium ion antagonist (isoptin). RESULTS: The influx of Ca 2+ APM and the proliferation was increased after insulin administration, but the proliferation was inhibited by isoptin. CONCLUSION: Changes in the homeostasis of calcium ion across plasma membrane was involved in the effect of insulin on the proliferation of human hepatocellular carcinoma.

Objective To explore the value of urinary N-terminal telopeptide of type Ⅰ collagen (uNTX) combined with bone scintigraphy (BS) for the diagnosis of bone metastases.Methods A total of 227 patients suspected of bone metastases by BS were selected from Jan to May of 2012.UNTX was tested for each subject.The threshold of uNTX was chosen as 65 nmol bone collagen equivalents (BCE)/mmol creatinine (Cr) for the diagnosis of bone metastasis.Patients with uNTX greater than 65 nmol BCE/mmol Cr would be diagnosed as having bone metastasis.Other examinations (CT,MRI,PET/CT or bone biopsy) were also performed to confirm the diagnosis.The uNTX values of benign and malignant bone lesions were compared using two-sample t test.The diagnostic efficacy of uNTX combined with BS was evaluated.A ROC curve was analyzed to evaluate the cut-off value of uNTX for the diagnosis of bone metastasis.Results The mean value of uNTX of all 227 patients was (84.30± 13.29) nmol BCE/mmol Cr,which was significantly higher than the upper limit of normal range (t =21.875,P＜0.01).Using 65 nmol BCE/mmol Cr as the threshold,197 cases were diagnosed as with and 30 without bone metastases,in which 188 and 27 were respectively confirmed by other examinations.The mean uNTX was (88.73 ± 8.37) and (60.76± 9.14) nmol BCE/mmol Cr in patients with and without bone metastases,respectively (t =-18.134,P＜0.01).The sensitivity,specificity,positive predictive value,negative predictive value,accuracy and Youden index of uNTX combined with BS were 98.4％ (188/191),75.0％ (27/36),95.4％ (188/197),90.0％ (27/30),94.7％ (215/227) and 73.4％ respectively.The cut-off value by ROC curve analysis was 78.88 nmol BCE/mmol Cr and the area under the curve was 0.982.Using 78.88 nmol BCE/mmol Cr as threshold,the corresponding sensitivity,specificity,positive predictive value,negative predictive value,accuracy and Youden index were 97.4％(186/191),94.4％(34/36),98.9％(186/188),87.2％(34/39),96.9％(22/227) and 91.8％ respectively.Conclusions Using the cut-off value of 78.88 nmol BCE/mmol Cr,uNTX may have clinical value in helping the differential diagnosis of bone scintigraphy for patients suspected of metastatic bone disease.

OBJECTIVETo observe the aging law on anti-hypertensive effect of scrapping therapy on primary hypertension so as to provide the reference evidence for the best treatment frequency of scrapping therapy.

METHODSEighty-nine patients of primary hypertension complied with inclusive criteria were treated with scrapping therapy according to syndrome differentiation. The stimulated sites included the courses of the governor vessel and the bladder meridian on the neck and back region, the line from Quchi (LI 11) to Shousanli (LI 10) and that from Zusanli (ST 36) to Fenglong (ST 40). The strong stimulation for reducing attempt was applied to the case of excessi syndrome, determined by subcutaneous capillary rupture and subcutaneous blood stasis. The mild stimulation for reinforcing attempt was applied to the case of deficiency syndrome, determined by subcutaneous capillary hyperemia and skin flush. Each site was scrapped for 10 times, about 5 cm in width, for 15 min. One scrapping treatment was required. The blood pressure was taken as the observation index separately, named 2:00 pm, 2:30 pm, 3:00 pm, 3:30 pm, 4:00 pm, 5:00 pm, 6:00 pm and 8:00 pm one day before scrapping, the time before scrapping in the afternoon on the day of treatment, the moment (2:00 pm), 2:30 pm, 3:00 pm; 3:30 pm, 4:00 pm, 5:00 pm, 6:00 pm and 8:00 pm after scrapping, in 24 h and 48 h after scrapping.

RESULTS(1) After one scrapping, the blood pressure was reduced to be (137. 51±10. 24)/(81. 06±10. 56) mmHg half a hour after scrapping from (149. 00±10. 19)/(85. 30±10. 96) mmHg (1mmHg~0. 133 kPa) before scrapping (P<0. 01). (2) The differences were significant in blood pressure at each time point after scrapping as compared with that before scrapping (all P<0. 01). (3) Through nonlinear regression analysis, the systolic blood pressure after scrapping was increased up to 140 mmHg in 47. 2 h after scrapping.

CONCLUSIONScrapping therapy achieves the apparent immediate anti-hypertensive effect on primary hypertension. It is suitable to apply scrapping treatment once every other day.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Blood Pressure ; Essential Hypertension ; Female ; Humans ; Hypertension ; physiopathology ; therapy ; Male ; Meridians ; Middle Aged

To select the most effective parameters of electroacupuncture (EA) at Hegu (LI4) and Sanyinjiao (SP6), a traditional acupuncture formula that induces labor, by comparing its effects on uterine contraction in late-stage pregnant rats, so as to improve the effects of acupuncture on induction of labor and its clinical maneuverability and provide the basis for further mechanism research.

It's been confirmed that acupuncture-moxibustion is effective in treating primary dysmenorrhea, and the efficacy is closely related to the intervention time. By summarizing the latest research progress on real-time intervention and pre-intervention of acupuncture-moxibustion in treating primary dysmenorrhea, this articlewas to discuss about the effect of intervention time on the efficacy in treating primary dysmenorrhea and thus to provide evidence to choose the optimal intervention time. It brought up that real-time intervention of acupuncture-moxibustion can produce a significant analgesic effect in treating primary dysmenorrhea, while pre-intervention of acupuncture-moxibustion presents its advantages in generally regulating the body function. Therefore, the treatment of primary dysmenorrhea should combine real-time intervention and pre-intervention of acupuncture-moxibustion to enhance the clinical efficacy.

Objective To investigate the application value of the preoperative progressive pneumoperitoneum (PPP) in parastomal hernia repair.Methods The retrospective cross-sectional study was conducted.The clinical data of 28 patients who underwent parastomal hernia repair using PPP in the Sixth Affiliated Hospital of Sun Yat-sen University from December 2014 to February 2017 were collected.Patients received abdominal computed tomography (CT) scan after admission,and volumes of the hernia sac and abdominal cavity and (volume of the hernia sac / total volume of the abdominal cavity)× 100.0％ were respectively calculated.Open or laparoscopic parastomal hernia repair was selected based on the effects of artificial pneumoperitoneum.Observation indicators:(1) PPP situations:① completion;② changes of volumes of the hernia sac and abdominal cavity before and after PPP;③ adhesion and retraction of parastomal hernia contents after PPP;(2) surgical and postoperative recovery situations;(3) follow-up situations.Follow-up using outpatient examination and telephone interview was performed to detect the postoperative long-term complications and recurrence of parastomal hernia up to May 2017.Measurement data with normal distribution were represented as (x)±s.Measurement data with skewed distribution were described as M (range).Repeated measurement data were evaluated with the repeated measures ANOVA.Results (1) PPP situations:① completion:28 patients received successful ultrasound-guided indwelling catcher.Twenty-four patients completed PPP,with a completion rate of 85.7％ (24/28) and an air injection volume of (3 995±531) mL,and 4 stopped PPP.Eighteen patients had varying degrees of abdominal pain,abdominal distension and scapular pain,including 17 with tolerance and 1 with disappearing of symptoms at day 6.Of 5 patients with shortness of breath,3 were improved or well tolerated through breathing exercises,and symptoms of 2 disappeared at day 7 and 9.Three patients had mild subcutaneous emphysema.The arterial CO2 tension of 1 patient was high and then returned to normal at day 7.Some patients had simultaneously multiple adverse reactions.② Changes of volumes of the hernia sac and abdominal cavity before and after PPP:volumes of the hernia sac before and after PPP were (699± 231) mL and (993 ± 332) mL,with a statistically significant difference (F=129.29,P＜0.05),and increasing volume of the hernia sac was (294± 167) mL,with an increasing rate of 43％±15％.Volumes of the abdominal cavity before and after PPP were (6 520±745)mL and (9 196± 909) mL,with a statistically significant difference (F=429.42,P＜0.05),and increasing volume of the abdominal cavity was (2 715±709)mL,with an increasing rate of 42％± 12％.(Volume of the hernia sac / total volume of the abdominal cavity) × 100.0％ before and after PPP were 9.6％ ± 2.7％ (less than or equal to 10.0％ in 20 patients,more than 10.0％ and less than or equal to 15.0％ in 6 patients,and more than 15.0％ in 2 patients) and 9.7％± 2.8％,with no statistically significant difference (F =0.44,P＞0.05).③ Adhesion and retraction of parastomal hernia contents after PPP:results of abdominal CT showed anterior abdominal bulging,abdominal contents prostrated at the base of the abdominal cavity due to gravity,and gas was full of gaps.Abdominal adhesion signs:adhesions of banded fibrous connective tissue established a connection between the base of the abdominal cavity and anterior abdominal wall,and intestinal canals were found inside the adhesions.Parastomal hernia contents of 28 patients had varying degrees of retraction to abdominal cavity,including 9 with complete retraction,13 with a great amount of retraction (retraction volume ＞50％) and 6 with a small amount of retraction (retraction volume ＜50％).Four patients were accompanied by incomplete stoma obstruction,and then obstruction disappeared or relieved after PPP.(2) Surgical and postoperative recovery situations:all the 28 patients underwent successful operations,without intestinal canal injury.Three patients received open parastomal hernia repair,including 2 receiving preperitoneal mesh repair using 8 layers Biodesign meshes (deep venous catheter for local drainage was placed and then removed at postoperative day 2 and 3) and 1 receiving Sugarbaker surgery using PCOPM mesh (peritoneal drainage-tube was placed and then removed at postoperative day 2).Other 25 patients received laparoscopic parastomal hernia repair and Sugarbaker surgery using PCOPM and Sepramesh meshes (no drainage-tube was placed).Bladder pressure of 28 patients at postoperative day 3 was (13±6)cmH2O (1 cmH2O =0.098 kPa),without an abnormal high pressure.Nine patients with postoperative complications were improved by conservative treatment,including 3 with seroma,3 with delayed stoma defecation or incomplete intestinal obstruction,2 with pulmonary infection and 1 with urinary tract infection.There were no occurrences of abdominal compartment syndrome,cardiac failure,lung failure,renal failure,other severe complications and perioperative death.Duration of postoperative hospital stay was (7.2± 1.5) days.(3) Follow-up situations:25 of 28 patients were followed up for 3-25 months,with a median time of 11 months.During follow-up,2 patients had chronic pain around the operation and a sense of discomfort and then were improved by symptomatic treatment,and 1 with parastomal hernia recurrence at postoperative month 6 after open preperitoneal mesh repair underwent again open preperitoneal mesh repair,without recurrence.There were no occurrence of tardive mesh infection and other longterm complications.Conclusion PPP in the treatment of parastomal hernia repair is safe and feasible.