1.Clinical significance of miR-143 and miR-145 expression in gastric gastrointestinal stromal tumor
Zhi LIU ; Shaoliang HAN ; Yingpeng HUANG ; Wenyi WU ; Junlin LI ; Xiangyang XUE ; Xian SHEN
Chinese Journal of General Surgery 2010;25(8):678-680
Objective To evaluate the role of miR-143, miR-145 in the development of gastric gastrointestinal stromal tumor. Methods The expression levels of miR-143 and miR-145 in 21 cases of gastric gastrointestinal stromal tumor and the matched non-tumor adjacent tissue specimens were examined by stem-loop real-time RT-PCR, and its correlation with clinicopathologic features of gastric gastrointestinal stromal tumor were analyzed. Results Expression level of miR-145 were significantly higher in tumor than adjacent normal tissues (P<0.01 ) and that with mitotic count ≥ 5/50HPF cases was significantly lower than that with mitotic count <5/50HPF cases (P=0.02). miR-145 expression in huge tumor (>10 cm)was significantly lower than that in the large tumor (5~10 cm) and small tumor (2~5 cm) (P=0.048).By Fletcher risk stratification system, miR-145 expression in high-risk cases was significantly lower than that in the intermediate-risk and low-risk cases (P=0.048). While the expression of miR-145 in low-risk group was significantly different compared to that in intermediate-risk group and high-risk group (P=0.01).There was no difference between the expressions of miR-143 in tumor and that in normal tissue(P=0.06).Conclusion In gastric gastrointestinal stromal tumor, MiR-145 expression is significantly higher in tumor than adjacent normal tissues. miR-145 is closely associated with tumor size. mitotic counts and Fletcher risk stratification system.
2.Study on Improvement Effects of Icariside Ⅱ on Neurological Function of Focal Cerebral Ischemia Model Rats by Regulating miR- 141-3p/Notch/Nrf2 Axis
China Pharmacy 2020;31(19):2386-2391
OBJECTIVE:To study the improvement effects of icariside Ⅱ(ICS Ⅱ)on neurological function of focal cerebral ischemia model rats by regulating miR- 141-3p/Notch/nuclear factor erythroid- 2-related factor 2(Nrf2)axis(miR-141-3p/Notch/ Nrf2). METHODS :The rats were divided into sham operation group ,model group ,nimodipine group (20 mg/kg)and ICS Ⅱ low-dose,medium-dose and high-dose groups (4,8,16 mg/kg),with 20 rats in each group. Twenty-four hours after establishing focal cerebral ischemia model ,model rats were given re levant medicine or normal saline intragastrically ,twice a day ,for consecutive 3 d. The neurological deficit of rats in each group was scored ;the volume of cerebral infarction was measured by 2,3, 5-triphenyltetrazolium chloride (TTC)staining;water content of cerebral tissue and the permeability of blood-brain barrier were measured;HE staining was performed to observe the pathological change of cerebral tissue of rats ;the expression of miR- 141-3p in cerebral tissue of rats was measured by qRT-PCR ;the protein expression of Notch and Nrf 2 in cerebral tissue of rats were measured by Western blotting assay. RESULTS :Compared with sham operation group ,the neurological deficit score ,expression of Notch-1 and Nrf 2 in model group were significantly lowered (P<0.05);infarction volume ,brain water content ,the permeability of blood-brain barrier and the expression of miR- 141-3p in cerebral tissue were increased significantly (P<0.05);the distribution of cortical cells was disordered ,and inflammatory infiltration and necrosis were observed in a large number of nerve cells. Compared with model group ,the neurological deficit score ,the protein expression of Notch- 1 and Nrf 2 in cerebral tissue were significantly increased in ICS Ⅱgroups(P<0.05);infarction volume ,brain water content ,the permeability of blood-brain barrier and the expression of miR- 141-3p in cerebral tissue were decreased significantly (P<0.05);the arrangement of cortical cells was regular,and the inflammatory infiltration and necrosis of nerve cells were decreased significantly. CONCLUSIONS :ICS Ⅱ can promote the recovery of neurological function in focal cerebral ischemic model rats ,which may be related to down-regulation of miR-141-3p and activation of Notch/Nrf 2 axis.