Objective To investigate the apoptosis induction of Bullatacin on A549 cell line of pulmonary adenocarcinoma. Methods The MTT assay was used to detect the growth inhibition rates of A549 cells cultured with Bullatacin in different concentrations (6.25, 12.5, 25, 50, 100μg/mL). 25μg/mL Bullatacin was used to culture A549 cells for 0, 12, 24, 48 h. The cell cycle distribution and apoptosis were measured by flow cytemetry. The protein expressions of ERK, JNK, and p38 were studied by Western blot. Results Dosage dependence was obviously showed after the different concentrations of Bullatacin were used to A549, and 25 μg/mL;Bullatacin blocked A549 cell in G0/G1 periods and induced its apoptosis. Compared with the blank group, protein expressions of P-ERK, P-JNK, and P-p38 were all increased by different degrees. Conclusion Bullatacin significantly inhibits the proliferation and induces the apoptosis of A549 cell. Its mechanism is related to activity of MAPK pathway thought the phosphorylation of the three protein kinases by Bullatacin.

Objective Through retrospective analysis of the causes of organ damage after in vitro fertilization embryo transfer,we put forward targeted prevention and take corresponding nursing measures to promote the prognosis of patients. Methods Selected 14 patients who suffered from organ damage after paracentes is in vitro fertilization and embryo transfer in our hospital.The causes were analyzed,and the treatment and nursing protocols were determined according to the kind and severity of the injured organs. Results 14 cases of organ injury patients with treatment and nursing gauze to stop bleeding, antibiotics, conservative treatment, hemostatic anti shock, indwelling catheter bladder irrigation, ureteral catheter and surgery were given symptomatic, were cured, hospitalization time was 3- 10 days. Conclusions The reasons for such patients, clinical observation and analysis of nursing treatment, let clinical nurses accumulate nursing experience in such patients, can timely take corresponding nursing measures,In order to improve the related nursing level of assisted reproductive technology.

Through summarizing and analyzing the characteristics of elderly infertile people,the problems emer-ging in the assisted reproduction process and the possible ethical problems emerging in the assisted reproduction treatment of elderly patients,this paper explored how to build the ethical path which aimed at elderly pregnancy -assisted people and suitable for Reproductive Center in the First Affiliated Hospital of Xinjiang Medical University. And aiming at the possible ethical problems emerging in the process of assisted reproduction treatment of elderly pa-tients,this paper put forward that it should establish normative ethical working path,to be more convenient to fully conduct ethical supervision and examination in the process of assisted reproduction treatment of elderly patients.