Objective:To evaluate the outcome of implant-supported all-ceramic fixed partial prostheses (FPPs)in posterior area. Methods:The clinical data of 1 20 implants-supported 53 fixed dentures in 47 patients were collected from July 201 1 to June 201 2 and prospectively studied.Complication and failure of implants and /or prosthesis,biological and technical complications were evaluated. Results:43 restorations with 1 00 implants in 37 cases were followed up for 1 2 -24 months.Veneering ceramic chipping was observed in 9(20.9%)prostheses.Inflamed marginal gingivitis was found around 3(7.0%)prostheses.No implant was involved in technical complication.Cumulative survival rate was 1 00% for implant-based analysis and 1 00% for prostheses-based analysis.Conclusion:Implant-supported all-ceramic fixed partial dentures may be a feasible treatment modality for posterior dental restoration.

This study is to establish a simple and practical co-culture method of cortical neurons and astrocytes of rats. The cortex of the new-born SD rats was digested by 0.125% pancreatic enzyme, and the differential adherence was applied to obtain the mixed cell suspension of neurons and astrocytes. A low concentration of cytarabine was used to inhibit the astrocytes in a moderate way to get neuronal and astrocyte co-culture. The morphological characteristics of the cells in different times were observed under the inverted microscope. The cells began to adhere the wall 2 h after the inoculation. Neurons and astrocytes grew in a good condition under the inverted microscope 9 days after the inoculation. The results of the immunofluorescence staining and Rosenfeld's staining indicated that the co-culture of neurons and astrocytes was successful and the ratio of neurons and astrocytes was close to 1:1. A new neurons and astrocytes co-culture method, which is simple and convenient, was successfully established. It will be an efficient method for the related researches about neuronal and astrocyte co-culture in vitro.

OBJECTIVETo investigate the effect of human serum albumin (HSA) on cell attachment of human gingival epithelial cells (HGE).

METHODSHGE were primary cultured with keratinocyte serum-free medium (KSFM) and dispase. The cultured cells were immunohistochemically stained by monoclonal anti-pan cytokeratin. MTT test was employed to investigate the influence of HSA on the cell attachment on polystyrene surface. The cell growth curve of HGE which were cultured in KSFM with 50 g/L HSA was observed.

RESULTSThe results showed significant decrease in cell numbers within 8 hours after HGE were inoculated, in which the polystyrene surface was preincubated with 50 g/L HSA. But it did not prove to be the case from 10 hours to 24 hours after HGE were inoculated. There were no significant difference within 24 hours in cell numbers between cultured in KSFM with 50 g/L HSA and control. The cell numbers in cell growth curve of HGE in KSFM with and without 50 g/L HSA did not show significant difference.

CONCLUSIONSHSA preincubation on polystyrene were produce inhibitory effect of HGE attachment in early stage.

Cell Adhesion ; drug effects ; Cell Count ; Cell Division ; drug effects ; Cells, Cultured ; Epithelial Cells ; cytology ; drug effects ; Gingiva ; cytology ; drug effects ; Humans ; Polystyrenes ; Serum Albumin ; pharmacology

Objective To evaluate the clinical and radiographic outcomes of placing dental implants in the posterior maxilla using the transalveolar technique.Methods Between January 2005 and December 2009,a total of 94 cases,55 males and 39 females,aged (46.9 ± 11.8) years,were consecutively enrolled in this study with maxillary sinus floor augmentation using the transalveolar technique.Radiographic evaluations were conducted on panoramic and periapical radiographs at each recall.The follow-up clinical examination included cumulative survival rate(CSR) of implants,peri-implant marginal bone loss(MBL) and the height of sinus floor augmentation and the endo-sinus bone gain(ESBG).Results Sinus membrane perforation was found in 4 cases.Finally 126 implants (90 cases) were placed.The perforation rate of this surgical procedure was 3.08％ (4/130).Four implants lost during the healing time,the early success of implants was 96.80％ (121/125).During a mean follow-up time of (34.4 ± 20.4) months,all the implants were successfully in function,with the cumulative survival rate after function loading of 100.00％.The mean MBL was (0.75 ± 0.51) mm during the healing time,and (0.48 ±0.41) mm during the follow-up period.The mean residual bone height(RBH) was (7.27 ± 1.30) mm at the position of implants placement,the length of implant protruding into the sinus was (2.77-± 1.15) mm,and the height of sinus floor augmentation was (4.52 ± 1.39) mm.At the loading time,the mean endo-sinus bone gain was (3.81 ± 1.60) mm.After the follow-up time of 34 months,the newly formed bone on the maxillary sinus floor underwent further remodeling with a median bone reduction value of 0.37 (0.10,0.88) mm.Conclusions Maxillary sinus floor augmentation using the transalveolar technique is a predictable treatment modality.The augmentation of the maxillary sinus floor using the transalveolar technique could lead to bone formation under the sinus.The newly formed bone showed slight absorbtion in the long term follow-up.

Objective To investigate the relationship between the expression of Krüppel-like factor 5(KLF5)and lysine demethylase 2A(KDM2A)in cancer tissues of patients with oral cancer and clinicopatho-logical characteristics and prognosis.Methods Cancer tissues of 80 patients with oral cancer who underwent surgical treatment in the Zigong First People's Hospital from January 2015 to January 2018 were retrospec-tively collected as research objects,and normal adjacent tissues were selected as controls.The expression of KLF5 and KDM2A in cancer tissues and adjacent tissues of patients with oral cancer was detected by immuno-histochemical staining,and the correlation between KLF5 and KDM2A in cancer tissues was analyzed by Spearman correlation.Clinicopathological features of patients with oral cancer were collected,and the relation-ship between the expression of KLF5 and KDM2A in cancer tissues and clinicopathological features of patients was analyzed,as well as the prognostic factors of patients with oral cancer were analyzed by multivariate Cox regression.Kaplan-Meier method was used to analyze the relationship between the expression of KLF5 and KDM2A and the prognosis of patients with oral cancer.Results The high expression rates of KDM2A and KLF5 in oral cancer tissues were significantly higher than those in adjacent tissues,and the difference was sta-tistically significant(P＜0.05).Spearman correlation analysis showed that there was a positive correlation be-tween KLF5 and KDM2A expression in oral cancer patients(r=0.375,P＜0.05).The expressions of KLF5 and KDM2A in patients with oral cancer were correlated with TNM stage,clinical stage,lymph node metasta-sis,local invasion and differentiation degree(P＜0.05).Multivariate Cox regression analysis showed that the expression level of KLF5 and KDM2A,TNM stage,lymph node metastasis,local invasion and differentiation degree were independent risk factors for poor prognosis in patients with oral cancer(P＜0.05).The 5-year survival rates of patients with high expression of KLF5 and KDM2A in cancer tissues[38.88％(21/54),42.37％(25/59)]were lower than those of patients with low expression of KLF5 and KDM2A in cancer tis-sues[65.38％(17/26),61.90％(13/21)],the difference was statistically significant(x2=6.554,4.046,P＜0.05).Conclusion The expression of KLF5 and KDM2A in cancer tissues of patients with oral cancer is high and affects the prognosis of patients.