Pathologist Dixit in American Michigan University found a gene induced by cytokine from human umbilical vein endothelial cells in 1990.This gene is increased in a variety of cells rapidly and coded a special kind of the zinc finger protein when being stimulated by interleukin-1 (IL-1),PLS and tumor necrosis factor (TNF).The gene was named as zinc finger protein A20 gene,and its coding protein was named as zinc finger protein A20.A20 is a kind of endogenous regulatory protein,inhibiting the inflammatory reaction and cell apoptosis through the inhibition of NF-kappa B activity.This article mainly reviewed the structure,function and clinical significance of A20.

Objective To investigate the changes of serum homocysteine (Hcy) level before and after treatment in patients with colorectal cancer,and provide the reference for clinical therapeutic efficacy and prognosis.Methods Enzymatic circling assay was used to measure the serum Hcy levels in 50 controls and 58 patients with colorectal cancer before and after treatment with a.follow-up of 12 months.The changes of Hcy aud the relationship between Hcy and therapeutic efficacy or prognosis were analyzed.Results Scrum Hcy level in patients with colorectal cancer was significantly higher than that in controls [(16.90±5.35) μmol/L vs (10.23±3.06) μmol/L] (P ＜ 0.01),and it was closely associated with TNM stage.Serum Hcy level in patients of stage Ⅲ-Ⅳ was significantly higher than that of stage Ⅰ-Ⅱ [(18.49±5.13) μmol/L vs (15.20±4.86) μmol/L] (P ＜ 0.05).The Hcy level in patients at the end of treatment was significantly lower than that before treatment [(13.39±4.98) μmol/L vs (16.90±5.35) μmol/L] (P ＜ 0.01),and the Hcy level after the treatment for 3 month was significantly lower than that at the end of treatment [(10.23±3.17) μmol/L] (P ＜ 0.05).The Hcy level in patients with recurrence during 12 months after operation was significantly higher than that without recurrence [(17.18±4.82) μmol/L vs (12.36±3.19) μmol/L] (P ＜ 0.01).Conclusion Serum level of Hcy might be a useful marker for predicting therapeutic efficacy and prognosis in patients with colorectal cancer.

Objective To establish the quality standard for Yangxue Yishen Capsules. Methods Rhizoma Gastrodiae, Herba Epimedii and Radix et Rhizoma Salviae Miltiorrhizae in the formula were identified qualitatively by TLC. The content of Astragaloside IV was determined by HPLC-ELSD method. The Diamonsil C18(2) column (4.6 mm×250 mm, 5 μm) was used. The mobile phase was acetonitrile-water (33∶67) with 1.0 mL/min flow rate at 35 ℃. The temperature of drift tube for ELSD was 105 ℃, the air flow rate was 2.7 L/min. Results The TLC displayed clear spots on their right positions repeatedly. The linear range of Astragaloside IV was 0.397 0-5.955 0 μg (r=0.999 9). The average recovery was 102.0%, RSD was 1.5% (n=6). Conclusion The method is simple and quick. It can be used as quality control method of Yangxue Yishen Capsules.

This study was aimed to screen out a safe and effective traditional Chinese medicine (TCM) compound prescription in insomnia treatment, through the method of uniform design and comprehensive scoring method. It provided candidate drugs to further research and development of TCM new drug. U7(76) uniform design was used to divide groups. The test was given on sleep time prolonging effect of mice injected with pentobarbital sodium. The subliminal dosage of pentobarbital hypnosis was tested on mice injected with pentobarbital sodium. The acute toxicity (LD50) was tested on mice. Comprehensive scoring method was applied to evaluate the effect to determine the optimum dose. The results showed the optimized prescription was composed of Radix Sophorae Flavescentis and Schisandra chinensis. The optimum ratio was 1:3. It was concluded that the uniform design was applied in the experimental design with 3 factors and 6 levels. Comprehensive scoring method was used to evaluate the combined effect. The optimized prescription that put benefit and risk into consideration was composed of Radix Sophorae Flavescentis and Schisandra chinensis. The best ratio was 1:3, which can be further developed.

Translational medicine means that to establish a bridge between fundamental research and clinical medicine through bidirectional translation path in order to promote the translation and application from fundamental research to clinical medicine.The purpose of traditional Chinese medicine (TCM) translational model is to collate and build one multidimensional TCM translational model,rationality carry out the research on TCM translation.Three paths have been confirmed,where are from literature research to clinical verification,from clinical application to clinical verification,and from experiment to clinical verification.In this research,methodology of one path mcntioned above could be established,which is the key technology of translational medicine.Take constipation,insomnia,and post-flu cough as target diseases to investigate the herbal compounds which could be qualified for clinical application based on preliminary work.This research not only provided clinical data to TCM translation,but also established one methodology of TCM translation path--"from experiment to clinical verification".

To develop an intervention protocol for children's unintentional injury risk behaviors, and to evaluate the feasibility of the protocol.
 Methods: By theoretically analyzing the influential factors for children's unintentional injury risk behaviors, children's cognitive development characteristics and the social learning theory, an intervention protocol was established on the basis of changing the unintentional injury attribution and negative information transmission of risk behavior consequences. A primary school in Changsha city was selected by random cluster sampling. A community-based randomized controlled trial was conducted on the selected students once a week for 5 consecutive weeks. The scores of unintentional injury risk behavior before intervention, 3 months and 6 months after intervention, and the frequency before intervention and 6 months after intervention, were collected and compared.
 Results: A total of 194 children were included in the study: 98 in the intervention group; 96 in the control group; 96 (49.5%) boys and 98 (50.5%) girls between 7 and 8 years old. The scores of unintentional injury risk behavior for children in the intervention group at 3 and 6 months after intervention were 14.42±5.67 and 14.14±8.95, respectively, lower than those before the intervention (16.85±8.48) and in the control group (P=0.001). The number of minor unintentional injuries in the intervention group decreased from 119 to 56, and the number of children suffering 2 or more injuries dropped from 34 to 10 (P<0.001) at 6 months after the intervention, while both of them were lower than that in the control group (P=0.011). Similar changes were observed in some slight or more serious unintentional injuries (P=0.030).
 Conclusion: The protocol for changing the attribution to unintentional injury and negative information transmission for risk behavior consequences was proved to effectively reduce children's unintentional injury risk behaviors and relevant events.
Child ; China ; Cognition ; Female ; Humans ; Male ; Risk-Taking ; Schools ; Students ; Wounds and Injuries ; prevention & control

OBJECTIVES: To investigate the status and the related factors of nursing behaviors for pressure injury, and to provide the evidence for standardizing pressure injury management. METHODS: A total of 1 039 clinical nursing staff from 4 general hospitals in Changsha from December 1 to 30, 2017 were selected by a stratified random sampling procedure. Nurses' demographic information such as age, gender, title, educational attainment, and department were collected. We investigated the status of nursing behaviors on pressure injury by a self-designed questionnaire, assessed nurses' knowledge of pressure injury and nurses' attitude of pressure injury using the Pressure Ulcer Knowledge Test and Attitude towards Pressure Ulcer Prevention Instrument, respectively, compared the nursing behaviors on pressure injury with different backgrounds, used multiple linear regression to analyze the influential factors for nursing behaviors on pressure injury, and conducted the Pearson correlation analysis for nurses' knowledge, attitude, and behaviors on the pressure injury. RESULTS: The overall nursing behaviors score on pressure injury was 155.96±17.29. The 5 dimensional scores from high to low were: risk assessment (4.42±0.49), prevention actions (4.40±0.50), risk understanding (4.35±0.52), injury assessment and interventions (4.27±0.55), and health education (4.25±0.63). A significant difference was found in the nursing behavior scores of pressure injury among ages, lengths of service, education, and training times (all ˂0.05). There was no correlation between nurses' knowledge and behaviors (=0.606). The nurses' attitude was positively correlated with their behaviors (=0.307, ˂0.001), and the nurses' knowledge was also positively correlated with their attitudes (=0.212, ˂0.001). The results of multiple linear regression showed that the length of service (≤5 years), training times (1-2 times), education (diploma or below), the scores of nurses' knowledge, and the scores of nurses' attitude were independent influencial factors of nurses' behaviors on pressure injury. CONCLUSIONS The nursing staff in the general hospital of Changsha has a high level of nursing behaviors on pressure injury, and they has good sense of responsibility and confidence. However, personal competence in pressure injury is insufficient and still needs to be improved. The nursing managers should focus on the nurses' attitude and training frequency, increasing the experience in nursing the pressure injury and practical level, and arouse the highly educated nurses' enthusiasm and sense of accomplishment to prevent pressure injury, thus reducing the incidence of pressure injury.
Attitude of Health Personnel ; China ; epidemiology ; Cross-Sectional Studies ; Health Knowledge, Attitudes, Practice ; Hospitals, General ; Humans ; Nursing Staff, Hospital ; Pressure Ulcer ; epidemiology ; etiology ; Surveys and Questionnaires