Objective: To determine the inhibitory effects of anti-bone sialoprotein(BSP) antibody on human breast cancer cells adhering to bone matrix in vitro.Methods: Expression of BSP in 3 different cancer cell lines(bone seeking breast cancer cell MDA-MB-231-BO,lung adenocarcinoma cell SPCA-1 and colon carcinoma cell LOVO)was detected immunohistochemically.Cells adhering test was carried out to investigate the adhering of the 3 different cancer cell lines to mouse bone matrix in vitro and the inhibitory effect of anti-BSP antibody on MDA-MB-231-BO cells adhering to mouse bone matrix;Enzyme-linked immunosorbent assay was carried out for quantitative detection of TGF-?.Results: BSP immunostaining was positive in MDA-MB-231-BO cells and negative in SPCA-1 and Lovo cells.The number of MDA-MB-231-F10 cells adhering to mouse bone matrix was significantly more than SPCA-1 or Lovo cells(P

Objective To explore the influence of laparoscopic CO2 pneumoperitoneum on blood metastasis of colorectal cancer.Methods Sixty colorectal cancer patients were randomly divided into laparoscopic surgery group and open surgery group.RT-PCR was used to detect the expressions of CK20mRNA before treatment,in operation and after operation,and effect of different surgical methods on the metastasis of tumor cells in peripheral blood was analyzed.Results There were no significant differences in positive expression of CK20mRNA before treatment,in operation and after operation between two groups (P ＞ 0.05).There was no significant difference in 3-year survival rate between the two groups (P ＞ 0.05).Conclusion Laparoscopic CO2 pneumoperitoneum does not increase the positive rate of CK20mRNA in peripheral blood in treatment of patients with laparoscopic resection of colorectal cancer.

Objective To explore the influence of laparoscopic CO2 pneumoperitoneum on blood metastasis of colorectal cancer.Methods Sixty colorectal cancer patients were randomly divided into laparoscopic surgery group and open surgery group.RT-PCR was used to detect the expressions of CK20mRNA before treatment,in operation and after operation,and effect of different surgical methods on the metastasis of tumor cells in peripheral blood was analyzed.Results There were no significant differences in positive expression of CK20mRNA before treatment,in operation and after operation between two groups (P ＞ 0.05).There was no significant difference in 3-year survival rate between the two groups (P ＞ 0.05).Conclusion Laparoscopic CO2 pneumoperitoneum does not increase the positive rate of CK20mRNA in peripheral blood in treatment of patients with laparoscopic resection of colorectal cancer.