Thrombin activated fibrinolysis inhibitor ( TAFI) is a kind of plasma enzymes that can be activated by thrombin.TAFI regulates blood coagulation and fibrinolysis and has a strong fibrinolytic and anti-inflammatory ac-tivity.Its inhibitor is expected to minimize the risk of bleeding when thrombolytic recanalization .Also, studies found that TAFI played an important role in the development of cerebral thrombosis;atherosclerosis and so on .

Objective To investigate the relationship between pathological types of intracoronary thrombus and changes of ST segment in patients with acute myocardial infarction. Methods Thrombus aspiration during primary PCI was performed in 65 ST-elevation myocardial infarction (STEMI) patients and 42 non-ST-elevation myocardial infarction (NSTEMI) patients. Pathological analysis on thrombosis was made. Results Thrombi characteristics were collected from 48 STEMI patients and 12 NSTEMI patients. In the STEMI group, 39(81.3%) red/mixed thrombus and 9(18.7%) white thrombus were found. While in the NSTEMI group, 8(66.7%) white thrombus and 4(33.3%) red/mixed thrombus were obtained. Conclusions There are both red/mixed and white thrombus in the two kinds of acute myocardial infarction. A variety of elements, including thrombus formation, could contributes to the change of ST in electrocardiograph during acute myocardial infarction.

AIM: To study the effects of resveratral on lipid peroxidative reaction and reactive oxygen species (ROS) in experimental spinal cord injury rats. METHODS: The weight dropping method was used to produce the experimental spinal cord injury (SCI) in adult rats. Resveratrol (Res) and methylprednisolone (MPSS) were given intraperitoneally by the bolus injections of 50, 100 mg?kg -1 and 100 mg?kg -1 , respectively, immediately after induction of SCI. And then the effects of Res were observed at 1, 24, 48 h after SCI compared with MPSS. RESULTS: Res could obvious prevented reduction of superoxide dismutase (SOD) activity and reduce malondialdehyde (MDA) production and inhibit the ROS level in the injured spinal cord tissue in comparison with the SCI model at the dose of 50 mg?kg -1 and 100 mg?kg -1 , which the most remarkable effects was at 48 h with the inhibition rate over 40%. The effects of Res were the same as or even greater than those of MPSS. CONCLUSION: Res may effectively protect the spinal cord from lipid peroxidative reaction and ROS damage in SCI.

AIM To study the effects of resveratrol on secondary spinal cord edema and the activity of lactic dehydrogenase and Na +, K + ATPase in experimental spinal cord injury (SCI)rat. METHODS The weight dropping method was used to produce the experimental spinal cord injury in adult rats. Resveratrol (Res) and methylprednisolone (MPSS) were intraperitoneally by the bolus injections of 50 mg?kg -1 , 100 mg?kg -1 and 100 mg?kg -1 , respectively, immediately after the induction of SCI. And then the effects of Res were observed at 1 h, 24 h, 48 h after SCI compared with MPSS. RESULTS Res could obvious inhibit the secondary spinal cord edema with the most remarkable suppressing rate by 11 5% at 48 h. Res was able to significantly suppress the activities of the lactic dehydrogenase with the highest suppressing rate for over 40% at 24 h. In the meantime, Res markedly improved the Na +, K + ATPase activities that promoted to the biggest extent to 60% at 48 h. The effects of Res were the same as or even stronger than those of MPSS. CONCLUSION Res may effectively protect the spinal cord from secondary spinal cord edema and improve the energy metabolism system in SCI. It suggested that Res may be having potent therapeutic effect on SCI.

OBJECTIVE To investigate the bacterial contamination of office telephones and computers in an infectious disease hospital to provide evidence for their disinfection.METHODS A total of 206 bacterial samples were collected from 73 telephones and 30 computers in offices in our hospital to determine the bacterial contamination of these office tools.RESULTS The contamination rate was 100% and sanitary qualification rate 32.5%.The highest bacterial colony count was 46 CFU/cm2 and the lowest one 2 CFU/cm2,The sanitary qulification rate of the telephones and computers in clean region was only 30.95%.CONCLUSIONS There was severe bacterial contamination in office telephones and computers,which should be paid close attention to.

Resulting from the shape stability, metal vascular stent has limited the vascular retraction, and subsequently prevent the unfavorable vascular remodeling. However, the metal stent requires further anticoagulant therapy after implantation, induces the hyperplasy of vascular smooth muscle cells, and cannot completely prevent the occurrence of in-stent restenosis. Surface modification of metal stent may reduce thrombogenesis. Based on the metal stent, drug eluting stent can transfer the active drugs to the damaged vessels, release them into the vascular wall and inhibit the in-stent restenosis. The eluting drugs restrain both the proliferation of smooth muscle cells and the regeneration of normal endothelial cells, leading to delay the vascular endothelialization and increase the risk of delayed thrombogenesis. The effect of stent implantation on the modus and size of vascular injury varies according to different operational techniques, processing technologies and designs, thus influencing the occurrence of in-stent restenosis. It is a potential study topic of interventional therapy to develop new eluting materials and eluting drugs, modify formulation, as well as facilitate the stent structure.

Coronary stent can cause mechanical injury to tunica intima and stimulation to vessel wall, resulting to platelet and inflammatory cell aggregation and infiltration, release of inflammation mediators, chemotatic factor, adhesion molecule and growth factor, and promoting migration and proliferation of vascular smooth muscle cells. The inflammatory reaction post stenting is highly correlated with intravascular restenosis. The drug-eluting stent against proliferation of vascular smooth muscle cells and inflammatory reaction can reduce the intensity and duration of body inflammatory reaction, improve stent technique, relieve damage of stenting to vessel wall, and reduce incidence of intravascular restenosis.

Objective To carry out regularpersonnel capacity evaluation of quality control laboratory (QC Lab) staff,combinedwith the actual situation of the QC Lab and the requirement of national capacity assessment.Method Establish an evaluation program,including assessment items,frequency,eligibility criteria,etc,to effectively implement the program.Results Two evaluations were conducted for nine staff membersin the lab from 2015 to 2016.The pass rate of the four test items and operation of the instrumentwere 100％.The ability to solve the problem was variable among the staff.Conclusion According to the evaluation results,staff classification management and training will effectively promote the improvement of personnel capacity and ensure the quality of laboratory testing.

Objectives To evaluate the application of flow-through hybrization and gene chip (HybriMax) on human papillomavious (HPV) subtype detection in clinical.Method 3 086 sexually active females from out-patient department were selected for HPV subtype analysis using HybriMax. Cervical tissue samples were taken under the colposcope from 463 females who had cervical lesion for pathological analysis. The predictive value of HybriMax in cervical abnormality was compared with pathological results, which were used as a golden standard. DNA sequence in HPV E6/E7 region was performed among 80 females with HPV 16 positive by HybriMax to determine the accuracy of HybriMax.Results All 21 different subtypes were found and total HPV positive rate was 63.1%(1 947/3 086). Among the 21 HPV subtypes, 5 of them had a infection rate over 5% and they were HPH16(15.9%, 490/3 086), 58(11.2%, 346/3 086), 52(8.5%、261/3 086)、33(6.3%、195/3 086)、53(6.2%、192/3 086)、6(5.6%、172/3 086), and CP8304(5.0%、155/3 086). The sensitivity for high degree squamous intraepithelial dysplasia(HSIL, CINⅡ+CINⅢ) by HybriMax was 95.49%(95%CI=95.44%～98.27%), while specificity was 34.85%(95% CI= 32.59%～37.57%)、Positive predictive value for HSIL was 37.13%(95%CI=30.79%～40.96%),while negative predictive vale was 95.04%(95%CI=89.24%～98.44%). Eighty sequence results in E6/E7 region completely matched to HybriMax results.Conclusion HybriMax has a high accurate rate in HPV subtype diction with good sensitivity and specificity for HSIL and above. It is an effective method to detect HPV subtype in clinical.

Objective To observe the effect of ligustrazine (TMP) on the expression of connective tissue growth factor (CTGF) and osteopontin (OPN) in the renal tubulointerstitium of diabetic nephropathy (DN) rats. Methods Wistar rats were used to establish the models through intraperitoneal injection of onedose streptozotocin (STZ) and nephrectomy. Rats were divided into 5 groups at random: model group, blank control group, Lotensin (1.7 mg? kg- 1? d- 1) group, of high-and lowdosage of TMP groups (150, 50 mg? kg- 1? d- 1). Except the rats in blank group and model group, the rats received corresponding medicines according to the experimental design for 12 weeks.Volume of urine protein within 24 hours was examined in the 4th, the 8th and the 12 th week, OPN mRNA expression in the renal tubulointerstitium was detected by RT- PCR and CTGF was detected by means of immunohistochemistry. Results Compared with normal group, the volume of 24 h urine protein rised obviously (P