Objective To study the abnormalities in defecation of patient after laparoscopic cholecystectomy as well as the influencing factors. Method A self-designed questionnaire and the anxiety self-rating anxiety scale (SAS) were used to investigate the defecation conditions of patients after laparoscopic cholecystectomy. Results About 40.78% of the patients were normal in defecation after the surgery. Among those with abnormalities in postoperative defecation, 30.16%had diarrhea. The influenced factors included age, preoperative gallbladder function, daily diets and anxiety. Conclusions The abnormalities in defecation of the patients after laparoscopic cholecystectomy are common. The patients with young age, the gallbladder function before laparoscopic cholecystectomy and anxiety diet without limit need more attention.

10 and Foxp3 expression, reduce the IL-17 and ROR-γt expression and adjust Treg/Th17 immune balance; effect of 0.1 mg/mL FSN +0.1 mg/mL MTX is the most obvious.

Objective To compare difference of nt and aa of H gene between the measles virus strains(MVW) recently circulating and used vacci ne strain (Chang-47) in Liaoning province of China, and to study biological an d immunological characteristic and genotype of MVW and Chang-47. Method s The analysis of 1～1 800 nt and 1～600 aa abou t H gene of MVW and Chang- 47 vaccine strain was performed. Neutralization test was performed against MVW and Chang-47 vaccine strain in the sera of measles cases and children vaccinated after 30 days. Results The genoty pe of the MVW was different from genotypes previously described in other c ountries and vaccine strain (Chang-47) used in Liaoning province. It also showe d that 18～28 aa were distinct from aa of other genotype, but most of the importa nt biological and immunological sites were not changing except the site at aa 24 0 .Neutralization antibody titer GMT against MVW and Chang-47 strains were no sig n ificantly different in the sera of measles cases and the children vaccinated af te r 30 days, but antibody titers GMT of the measles cases sera against MVW or Cha ng-47 strain were much higher than children vaccinated. Conclusions There was nt variation in the MVW. Most of biological and immunological a a sites of the MVW and Chang -47 strains were the same and were not changing. C hang -47 vaccine may also protect from infection of the MVW.

Objective To investigate the preventive effects of midazolam and propofol on liver against hypoxia/reoxgenation(H/R) injury in rats.Methods Twenty-four Wistar rats of both sexes weighing 200-250g were randomized into four groups:control group(group A),hypoxia/reoxygenation(group B),H/R + midazolam(group C) and H/R + propofol(group D).The 8-iso-prostaglandin F2?(8-iso-PGF2?) levels in the hepatic tissue were determined by enzyme-linked immunosorbent assay(ELISA).The contents of nitric oxide(NO) and the activity of nitric-oxide synthase(NOS) in hepatic tissue were determined by biochemistry methods.Results After hypoxia and reoxgenation injury in rats,the 8-iso-PGF2? level of hepatic tissue in group B was significantly higher than that in group A(P

Objective To investigate the protective effects of propofol on liver against hypoxia/reoxgenation (H/R) injury in rats. Methods Twenty-four Wistar rats of both sexet weighing 200-250g were randomized into control group (group A), hypoxia/reoxygenation group (group B) and H/R + propofol group (group C). The 8-iso-prostaglandin F 2? (8-iso-PGF 2? ) level in the hepatic tissue was determined by enzyme-linked immunosorbent assay (ELISA). The content of NO and the activity of NOS were assessed by biochemical methods. Results In group B, the 8-iso-PGF 2? level in hepatic tissue was significantly higher than that in group A (P

Objective To assess the relationship between the concentration of total suspended particle (TSP) and cerebral-cardiovascular disease mortality of urban residents in Fushun city, China. Methods The data of cerebral-cardiovascular disease mortality and TSP concentration from 1999 to 2003 in Fushun city were collected. The association between TSP concentrations and the mortality of cerebral-cardiovascular diseases was analyzed using Poisson regression model adjusted for seasons, long-term patterns and meteorological variations using an ecological parametric method. Results As the concentration of TSP increased by 50 ?g/m3, OR of cerebral-cardiovascular disease mortality increase was 1.015 42 (95%CI=1.000 18-1.030 89) in the male group and 1.022 40 (95%CI=1.004 87-1.040 23) in the aged male group respectively, as 4-days lag TSP concentration increased by 50 ?g/m3, OR of cerebral-cardiovascular disease mortality increase was 1.008 26 (95%CI=1.000 57-1.016 02) in the whole people, 1.016 27 (95%CI=1.006 71-1.025 93) in the male group and 1.016 65 (95%CI=1.005 25-1.028 19) in the aged male group respectively. Conclusion Air pollution by TSP is considered as a risk factor for the increase of cerebral-cardiovascular disease mortality in Fushun city, China.

0 05) The parameters did not change significantly with increasing MAC in group D, whereas LESP, BrP and PVV decreased significantly in a dose dependent manner in group E (P

Codon is an important carrier of genetic information in organisms. In evolution, codon bias is formed in many organisms. In this study, the analysis of expressed genes in Lonicera japonica Thunb. is presented. The codon bias of genes with complete coding region is analyzed through CodonW. And we calculated the codon usage bias of hydroxycinnamoyl-CoA: quinate hydroxycinnamoyl transferase (HQT) genes. The results showed that L. japonica were bias toward the synonymous codons with A and T at the third codon position.

Objective To investigate the effect of probiotics preconditioning on intestinal mucosal barrier function in a rat model of cardiopulmonary bypass (CPB) . Methods Twenty-four adult male SD rats weighing 350-450 g were randomly divided into 3 groups ( n = 8 each) : group Ⅰ sham operation ( group S); group Ⅱ CPB and group Ⅲ probiotics + CPB. CPB was established between left carotid artery and right jugular vein and maintained for 60 min. Croup Ⅲ received intragastric instillation of probiotics 2 ml once a day for 7 consecutive days before CPB. Blood samples were collected at 2 h after CPB was terminated for determination of plasma D-lactate, TNF-and IL-6 concentrations and diamine oxidase (DAO) activity. Venous blood was obtained from portal vein for determination of LPS concentration and cultured. Liver, pancreas, spleen, kidney and mesenteric lymphnode (MLN) specimens were obtained under sterile condition and cultured for bacterial growth. The mucous membrane of small intestine was examined with electron microscope. Results CPB significantly increased plasma DAO activity, and D-lactate, IPS, TNF-α and IL-6 concentrations and the rate of bacteria-positive culture of portal venous blood, liver, MLN, lung and kidney in group Ⅱ as compared with group S. Probiotics preconditioning significantly attenuated the above-mentioned CPB-induced changes. Microscopic examination showed that probiotics preconditioning significantly ameliorated CPB-induced damage to the epithelium of the small intestine. Conclusion Probiotics preconditioning can significantly attenuate CPB-induced inflammatory response and protect the intestinal mucosal barrier function.

Objective To investigate the effects of probiotics on the plasma diamine oxidase (DAO) activity and D-lactate, IL-6 and IL-10 levels in patients undergoing open heart surgery under cardiopulmonary bypass (CPB) , trying to elucidate the mechanism of protective effect of probiotics against CPB- induced injury to intestinal mucosal barrier. Methods Forty ASA Ⅱ - Ⅲ patients of both sexes aged 30-64 yr weighing 40-80 kg undergoing open heart operation under CPB were randomized into 2 groups ( n = 20 each) : control group (group C) and probiotics group (group Y) . Group Y received Jinshuangqi (Bifid Triple Viable containing bifido-bacterium, lacto-bacillus, streptococcus thermophiles) 4 pills 3 times a day for 7 days before operation. Venous blood samples were taken from CVP line before operation (T1 ), at 10 min after aortic unclamping (T2 ) and at the end of CPB (T3 ) and at 2, 6, 18 h (T4,5,6) after operation for determination of plasma DAO activity and D-lactate, IL-6 and IL-10 levels.Results Plasma DAO activity, D-lactate, IL-6 and IL-10 levels were significantly increased after CPB was started at T2-6 as compared with the baseline values at T1 in both groups. Plasma DAO activity and D-lactate level were significantly lower at T2-5 , the plasma IL-6 level was significantly lower and plasma IL-10 level higher at T4,5 in group Y than in group C. Conclusion Probiotics can protect intestinal mucosal barrier in patients undergoing open heart surgery under CPB and attenuate inflammatory response.