Objective To study the therapeutic effect and mechanism of Jiangtang Mixture combined with ear acupuncture for type 2 diabetic insulin resistance(IR).Methods Totally 120 cases of type 2 diabetic IR were randomly and equally divided into western medicine(Metformin) group,Chinese medicine (Jiangtang Mixture) group and Chinese medicine with ear acupuncture group.The treatment lasted for one month.The therapeutic effect,fasting plasma glucose(FPG),fasting insulin(FINS),postprandial 2 hours plasma glucose(P2hPG),hemoglobin A1c(HbA1c),blood fat,insulin Sensitivity index(ISI) and insulin resistance index(IRI) were observed before and after treatment.Results The total effective rate in Chinese medicine with ear acupuncture group was 92.5%,which was superior to those in Chinese medicine group (P

BACKGROUND:Total knee arthroplasty for knee osteoarthritis accompanied with extra-articular deformity is stil chal enging. An individual replacement plan should be designed to obtain good mechanical axis and to recover suitable soft-tissue balance. OBJECTIVE:To observe the safety and feasibility of individual y designed one-stage total knee arthroplasty for knee osteoarthritis combined with ipsilateral femoral extra-articular deformity. METHODS:A total of eight patients with knee osteoarthritis combined with ipsilateral femoral extra-articular deformity were enrol ed in this study. According to preoperative plan, five patients underwent intra-articular compensatory osteotomy, and the remaining three received extra-articular corrective osteotomy. Multi-planar deformities as wel as soft-tissue conditions were recorded. Main outcome measures included the hospital for special surgery score, Western Ontario and McMaster Universities Arthritis Index score and alignments deviation. RESULTS AND CONCLUSION:The patients were averagely fol owed up for 29 months. The hospital for special surgery score changed from preoperative 32.5 points to postoperative 87.5 points. The Western Ontario and McMaster Universities Arthritis Index score was increased from preoperative 37.1 points to postoperative 88.8 points (P<0.05). Mean mechanical axis deviation (either varus or valgus) changed from preoperative 17.1° to postoperative 1.4° (P<0.05). Al cases with extra-articular corrective osteotomy healed within 3 months, and none had postoperative infection, loosening or venous thromboembolic events. One patient was with 5° residual knee anti-sheets. Results verified that one-stage total knee arthroplasty procedures are effective in the treatment of knee osteoarthritis accompanied with ipsilateral femoral extra-articular deformity in accordance with individual preoperative plan.

Objective To study the method of cryopreservation, and method of cultivation of hepatocytes of sucking pig. Methods Hepatocytes were isolated from sucking pig by two-step perfusion with collagenase using an extracorporeal perfusion apparatus. They were cryopreserved in liquid nitrogen for 4 months. They were thawed and seeded on gelatin coated bottom of a flask. The cells were covered with another layer of collagen. The exprssion of albumin mRNA, cellular morphology synthesis of urea, and content of oxalylacetylaminotransferase were determined in the collagen cultures. Results Thawed hepatocytes maintained their biological characters well after cryopreservation. The thawed hepatocytes could grow well in collagen gel, sandwich culture. In the 10-day cultures, typical cellular morphological and biological characteristics of cultured hepatocytes could be observed. Conclusion Hepatocytes stored in liquid nitrogen could maintain their activities for a long time. The hepatocytes could be cultivated in collagenous matrix, which provided an environment closely resembling that in nature, and their morphology and functions could be maintained.

Objective To explore the typing,precaution and treatment of the bone cement leakage caused by percutaneous vertebroplasty(PVP).Methods PVP was performed in 38 cases(11 men and 27 women) with vertebral angioma and vertebral body compression fracture with osteoporosis chosen from November 2002.Results All the patients were followed up for 8 to 16 months and the effects were evaluated by the standards carried out by World Health Organization(WHO).The rate of complete response(CR) plus partial response(PR) was 100 percent.The patients with bone cement leakage by PVP were divided into 3 groups which underwent different remedial methods respectively.Conclusion The bone cement leakage could be prevented through the preoperative preparation,choosing the anaesthesia and scouting the whole operation.The patients with TypeⅠ,Ⅱ and Ⅲ_a needn't treatment,but those with type Ⅲ_b must accept open-operation.Vertebral eggshell technology with open vertebroplasty was effective on the treatment of the bone cement leakage with type Ⅲ_b caused by PVP.

Fifty eight patients with amyloidosis diagnosed by biopsy were admitted to Beijing Friendship Hospital from January 1983 to September 2007 and their clinical data were analyzed retrospectively.Twenty of the 58 cases were systemic amyloidosis,and other 38 were local amyloidosis.In 20 cased of systemic amyloidosis,kidney was involved in 75% cases,liver was in 55%,nervous system Was in 55%and heart Was in 50%.The lesion of local amyloidosis was usually limited in one single organ.

BACKGROUND:Now the filling material which is used for the treatment of osteoporotic compression fractures is mainly polymethacrylic acid (PMMA),but the PMMA as a permanent foreign body cannot promote bone regeneration,and has a downward extension of the possibility of mechanical properties with time prolonging,so a new type of filling material used to substitute PMMA is looking.OBJECTIVE:To investigate the effect and image characteristics of different filling materials on percutaneous vertebroplasty performing on a goat model of osteoporotic compression fracture.METHODS:Three vertebral fracture models were established on osteoporosis goats by using an appropriate mechanical stress.Under the G-arm X-ray machine,the fractured vertebrae was performed with percutaneous vertebroplasty and then filled with polymethyl methacrylate (PMMA),self-setting calcium phosphate cement (CPC) and recombinant human bone morphogenetic protein-2(rhBMP-2)/self-calcium phosphate cement.RESULTS AND CONCLUSION:① A total of 36 vertebral bodies of 12 goats were successfully performed with percutaneous vertebroplasty,and there was no significant difference in filling quantity among three materials (3.5±1.8) mL (P>0.05).Bolus resistance of PMMA was strong,but operative time was short,and the image was great;the bolus resistance of self-CPC and rhBMP-2/self-CPC was weak,operative time was long,but there was no leakage placeholde.②CT scanning results on the 2~(nd) day after surgery demonstrated that the three materials were disorderly distributed in the vertebral body,showing high-density patching shaping.Six out of 36 vertebral bodies were leakage,which was induced by PMMA (n=4),self-CPC (n=1),and rhBMP-2/self-CPC (n=1).③ Scanning electron microscope indicated that all materials in the PMMA group were not able to closely bind with host bone,but self-CPC and rhBMP-2/self-CPC were able to closely bind with host bone.The results suggested that rhBMP-2/self-CPC is characterized by long-term operation,short time of bone substitute,and stabilization of reconstructed spine.

How to improve the teaching quality is urgent and significant at present.Based on reforming the teaching methods,the authors have studied the new methods of medical postgraduate examination since 2005 including the assessments on practical skills,the level of mastering knowledge,self learning and innovating ability and the criterion of giving a mark,which have been applied for three years and achieved the aim and the effect in clinical electro-cardiology teaching.

Objective To compare the curative effect between arthroscopic and open retrograde intramedullary interlocking nails in the treatment of femoral supracondylar fracture. Methods A total of 46 patients with femoral supracondylar fracture underwent the treatment by either arthroscopic (19 patients, Group A) or open (27 patients, Group B) retrograde intramedullary interlocking nails. Results The operation time, surgical blood loss and bone union time in the Group A were less than those in the Group B ( t =-4 193, -21 517 and -4 055, respectively; P =0 000). Follow-up for (10 3?3 6) months in the 46 patients revealed the results of therapy were, according to the Kolmert criteria, excellent in 15, good in 3, fair in 1 in the Group A while excellent in 7, good in 14, fair in 4 and poor in 2 in the Group B, without statistically significances between the two groups in the “good” or “excellent” rates (94 7% vs. 77 8%; ? 2 =1 345, P =0 246). Conclusions Use of arthroscopic retrograde intramedullary interlocking nails has advantages of shorter operating time, less blood loss and quicker bone union, benefiting the recovery of knee joint functions.

Objective: To explore the working methods of clinical pharmacists for improving the rationality of drug use through participating in individualized treatment practice for the patients with tumor.Methods: Clinical pharmacists participated in the individualized treatment of the patients with tumor, found out adverse drug interactions, adjusted dosage for the renal insufficiency patients, treated infection or pain and corrected inappropriate drug combination, etc.Results: Clinical pharmacists provided integrated pharmaceutical care and offered medical advice in accordance with the individualized information of patients, which improved the treatment effect and avoided potential adverse drug reactions or adverse events.Conclusion: Clinical pharmacists provide pharmaceutical care and participate in the performance of individualized treatment for cancer patients, which can effectively improve the level of drug treatment.

Objective To compare difference of nt and aa of H gene between the measles virus strains(MVW) recently circulating and used vacci ne strain (Chang-47) in Liaoning province of China, and to study biological an d immunological characteristic and genotype of MVW and Chang-47. Method s The analysis of 1～1 800 nt and 1～600 aa abou t H gene of MVW and Chang- 47 vaccine strain was performed. Neutralization test was performed against MVW and Chang-47 vaccine strain in the sera of measles cases and children vaccinated after 30 days. Results The genoty pe of the MVW was different from genotypes previously described in other c ountries and vaccine strain (Chang-47) used in Liaoning province. It also showe d that 18～28 aa were distinct from aa of other genotype, but most of the importa nt biological and immunological sites were not changing except the site at aa 24 0 .Neutralization antibody titer GMT against MVW and Chang-47 strains were no sig n ificantly different in the sera of measles cases and the children vaccinated af te r 30 days, but antibody titers GMT of the measles cases sera against MVW or Cha ng-47 strain were much higher than children vaccinated. Conclusions There was nt variation in the MVW. Most of biological and immunological a a sites of the MVW and Chang -47 strains were the same and were not changing. C hang -47 vaccine may also protect from infection of the MVW.