Objective To study the therapeutic effect and mechanism of Jiangtang Mixture combined with ear acupuncture for type 2 diabetic insulin resistance(IR).Methods Totally 120 cases of type 2 diabetic IR were randomly and equally divided into western medicine(Metformin) group,Chinese medicine (Jiangtang Mixture) group and Chinese medicine with ear acupuncture group.The treatment lasted for one month.The therapeutic effect,fasting plasma glucose(FPG),fasting insulin(FINS),postprandial 2 hours plasma glucose(P2hPG),hemoglobin A1c(HbA1c),blood fat,insulin Sensitivity index(ISI) and insulin resistance index(IRI) were observed before and after treatment.Results The total effective rate in Chinese medicine with ear acupuncture group was 92.5%,which was superior to those in Chinese medicine group (P

Cisplatin, an efficient anticancer agent, can trigger multiple apoptotic pathways in cancer cell. However, the signal transduction pathways in response to cisplatin-based chemotherapy are complicated, and the mechanism is not fully understood. Using fluorescence resonance energy transfer (FRET) technique, the molecular mechanism of cisplatin-induced apoptosis in living human lung adenocarcinoma cells (ASTC-a-1) were investigated. After cisplatin treatment, the recombinant pFRET-Bid and pSCAT-3 probes were used to determine the kinetics of Bid cleavage and Caspase-3 activation, respectively. The fluorescence probes Bid-CFP and DsRed-Mit were also used to detect the spatial and temporal changes of Bid in real-time in sub-cell level. The results showed that a cleavage of the Bid-FRET probe occurring at about 4～5 h after treated with 20 ?mol/L cisplatin. Cleavage of the Bid-FRET probe coincided with a translocation of tBid from the cytosolic to the mitochondria, and the translocation lasted about 1.5 h. At the anaphase of cell apoptosis, Caspase-3 was activated obviously as detected by FRET and Western blotting techniques. Using real-time single-cell analysis, it was observed the kinetics of Bid and Caspase-3 activation for the first time in living cells during cisplatin-induced apoptosis.

Objective: To explore the working methods of clinical pharmacists for improving the rationality of drug use through participating in individualized treatment practice for the patients with tumor.Methods: Clinical pharmacists participated in the individualized treatment of the patients with tumor, found out adverse drug interactions, adjusted dosage for the renal insufficiency patients, treated infection or pain and corrected inappropriate drug combination, etc.Results: Clinical pharmacists provided integrated pharmaceutical care and offered medical advice in accordance with the individualized information of patients, which improved the treatment effect and avoided potential adverse drug reactions or adverse events.Conclusion: Clinical pharmacists provide pharmaceutical care and participate in the performance of individualized treatment for cancer patients, which can effectively improve the level of drug treatment.

How to improve the teaching quality is urgent and significant at present.Based on reforming the teaching methods,the authors have studied the new methods of medical postgraduate examination since 2005 including the assessments on practical skills,the level of mastering knowledge,self learning and innovating ability and the criterion of giving a mark,which have been applied for three years and achieved the aim and the effect in clinical electro-cardiology teaching.

Objective:To clarify the mechanism of crisis serum' mediated gametocyte infectivity to the mosquito vector Methods:Observing the effects of mouse serum , which was obtained 5 days after P yoelii infection (D5 serum) on gametocyte infectivity by IFA and mosquito live feeds, and the production of IFN ??TNF ??IL 4 and NO - 2 in the hosts in vivo and in vitro by ELISA and Griess reaction And to investigate the ability of malaria parasitized red blood cell extract (PRBC extract) to induce NO Results:The development of the gametocytes from mice 5 days postinfection into ookinetes were completely inhibited D5 serum was not immediate to inhibit gametocyte development, which was injected intravenously into the mice 3 days after P yoelii infection But 4 h later after injection D5 serum stimulated the increasing IFN ? and NO production and inhibited gametocyte infectivity Moreover, PRBC extract showed the ability to induce NO Conclusion:Infected host serum blocks transmission of P yoelii via a nitric oxide dependent mechanism

Objective:To investigate the expression of antigens in Plasmodium yoelii sexual stage and transmission blocking effects of monoclonal antibodies(McAbs).Methods:Observing the effects of anti Pys25 McAb4 and anti Pys21 McAb10 on developmental course of parasites in mosquitoes by direct mosquito feeds on passively immunized P.yoelii infected mice and the expression of Pys21 and Pys25 from gametocytes to ookinetes in indicated culture times by IFA and Western blotting.Results:The transmission blocking activity of the anti Pys25 McAb4 was complete and more potent than that of the anti Pys21 McAb10.Both Pys25 and Pys21 were presented in whole developmental course from gametocytes to ookinetes.Furthermore,the expression of Pys25 appeared to be earlier than that of Pys21 on zygote surfact.Conclusion:Pys25 and Pys21 are target antigens of transmission blocking immunity and that anti Pys25 McAb4 has more significantly transmission blocking activity is related with the early stage expression of Pys25 on zygote surface.

Codon is an important carrier of genetic information in organisms. In evolution, codon bias is formed in many organisms. In this study, the analysis of expressed genes in Lonicera japonica Thunb. is presented. The codon bias of genes with complete coding region is analyzed through CodonW. And we calculated the codon usage bias of hydroxycinnamoyl-CoA: quinate hydroxycinnamoyl transferase (HQT) genes. The results showed that L. japonica were bias toward the synonymous codons with A and T at the third codon position.

Fifty eight patients with amyloidosis diagnosed by biopsy were admitted to Beijing Friendship Hospital from January 1983 to September 2007 and their clinical data were analyzed retrospectively.Twenty of the 58 cases were systemic amyloidosis,and other 38 were local amyloidosis.In 20 cased of systemic amyloidosis,kidney was involved in 75% cases,liver was in 55%,nervous system Was in 55%and heart Was in 50%.The lesion of local amyloidosis was usually limited in one single organ.

BACKGROUND:Now the filling material which is used for the treatment of osteoporotic compression fractures is mainly polymethacrylic acid (PMMA),but the PMMA as a permanent foreign body cannot promote bone regeneration,and has a downward extension of the possibility of mechanical properties with time prolonging,so a new type of filling material used to substitute PMMA is looking.OBJECTIVE:To investigate the effect and image characteristics of different filling materials on percutaneous vertebroplasty performing on a goat model of osteoporotic compression fracture.METHODS:Three vertebral fracture models were established on osteoporosis goats by using an appropriate mechanical stress.Under the G-arm X-ray machine,the fractured vertebrae was performed with percutaneous vertebroplasty and then filled with polymethyl methacrylate (PMMA),self-setting calcium phosphate cement (CPC) and recombinant human bone morphogenetic protein-2(rhBMP-2)/self-calcium phosphate cement.RESULTS AND CONCLUSION:① A total of 36 vertebral bodies of 12 goats were successfully performed with percutaneous vertebroplasty,and there was no significant difference in filling quantity among three materials (3.5±1.8) mL (P>0.05).Bolus resistance of PMMA was strong,but operative time was short,and the image was great;the bolus resistance of self-CPC and rhBMP-2/self-CPC was weak,operative time was long,but there was no leakage placeholde.②CT scanning results on the 2~(nd) day after surgery demonstrated that the three materials were disorderly distributed in the vertebral body,showing high-density patching shaping.Six out of 36 vertebral bodies were leakage,which was induced by PMMA (n=4),self-CPC (n=1),and rhBMP-2/self-CPC (n=1).③ Scanning electron microscope indicated that all materials in the PMMA group were not able to closely bind with host bone,but self-CPC and rhBMP-2/self-CPC were able to closely bind with host bone.The results suggested that rhBMP-2/self-CPC is characterized by long-term operation,short time of bone substitute,and stabilization of reconstructed spine.

Objective To compare difference of nt and aa of H gene between the measles virus strains(MVW) recently circulating and used vacci ne strain (Chang-47) in Liaoning province of China, and to study biological an d immunological characteristic and genotype of MVW and Chang-47. Method s The analysis of 1～1 800 nt and 1～600 aa abou t H gene of MVW and Chang- 47 vaccine strain was performed. Neutralization test was performed against MVW and Chang-47 vaccine strain in the sera of measles cases and children vaccinated after 30 days. Results The genoty pe of the MVW was different from genotypes previously described in other c ountries and vaccine strain (Chang-47) used in Liaoning province. It also showe d that 18～28 aa were distinct from aa of other genotype, but most of the importa nt biological and immunological sites were not changing except the site at aa 24 0 .Neutralization antibody titer GMT against MVW and Chang-47 strains were no sig n ificantly different in the sera of measles cases and the children vaccinated af te r 30 days, but antibody titers GMT of the measles cases sera against MVW or Cha ng-47 strain were much higher than children vaccinated. Conclusions There was nt variation in the MVW. Most of biological and immunological a a sites of the MVW and Chang -47 strains were the same and were not changing. C hang -47 vaccine may also protect from infection of the MVW.