1.Jiangtang Mixture Combined with Ear Acupuncture for Type 2 Diabetic Insulin Resistance:A Clinical Observation of 40 Cases
Journal of Traditional Chinese Medicine 1993;0(03):-
Objective To study the therapeutic effect and mechanism of Jiangtang Mixture combined with ear acupuncture for type 2 diabetic insulin resistance(IR).Methods Totally 120 cases of type 2 diabetic IR were randomly and equally divided into western medicine(Metformin) group,Chinese medicine (Jiangtang Mixture) group and Chinese medicine with ear acupuncture group.The treatment lasted for one month.The therapeutic effect,fasting plasma glucose(FPG),fasting insulin(FINS),postprandial 2 hours plasma glucose(P2hPG),hemoglobin A1c(HbA1c),blood fat,insulin Sensitivity index(ISI) and insulin resistance index(IRI) were observed before and after treatment.Results The total effective rate in Chinese medicine with ear acupuncture group was 92.5%,which was superior to those in Chinese medicine group (P
2.Characteristic analysis of hemagglutinin gene of measles virus recently circu lating in Liaoning province of China
Yingjie SUN ; Chunmei LIU ; Jin LI
Chinese Journal of Disease Control & Prevention 2001;5(2):99-101
Objective To compare difference of nt and aa of H gene between the measles virus strains(MVW) recently circulating and used vacci ne strain (Chang-47) in Liaoning province of China, and to study biological an d immunological characteristic and genotype of MVW and Chang-47. Method s The analysis of 1~1 800 nt and 1~600 aa abou t H gene of MVW and Chang- 47 vaccine strain was performed. Neutralization test was performed against MVW and Chang-47 vaccine strain in the sera of measles cases and children vaccinated after 30 days. Results The genoty pe of the MVW was different from genotypes previously described in other c ountries and vaccine strain (Chang-47) used in Liaoning province. It also showe d that 18~28 aa were distinct from aa of other genotype, but most of the importa nt biological and immunological sites were not changing except the site at aa 24 0 .Neutralization antibody titer GMT against MVW and Chang-47 strains were no sig n ificantly different in the sera of measles cases and the children vaccinated af te r 30 days, but antibody titers GMT of the measles cases sera against MVW or Cha ng-47 strain were much higher than children vaccinated. Conclusions There was nt variation in the MVW. Most of biological and immunological a a sites of the MVW and Chang -47 strains were the same and were not changing. C hang -47 vaccine may also protect from infection of the MVW.
3.One-stage total knee arthroplasty for knee osteoarthritis combined with ipsilateral femoral extra-articular deformity:individual design
Pengcheng LIU ; Yingjie DENG ; Rui FANG
Chinese Journal of Tissue Engineering Research 2014;(26):4108-4114
BACKGROUND:Total knee arthroplasty for knee osteoarthritis accompanied with extra-articular deformity is stil chal enging. An individual replacement plan should be designed to obtain good mechanical axis and to recover suitable soft-tissue balance. OBJECTIVE:To observe the safety and feasibility of individual y designed one-stage total knee arthroplasty for knee osteoarthritis combined with ipsilateral femoral extra-articular deformity. METHODS:A total of eight patients with knee osteoarthritis combined with ipsilateral femoral extra-articular deformity were enrol ed in this study. According to preoperative plan, five patients underwent intra-articular compensatory osteotomy, and the remaining three received extra-articular corrective osteotomy. Multi-planar deformities as wel as soft-tissue conditions were recorded. Main outcome measures included the hospital for special surgery score, Western Ontario and McMaster Universities Arthritis Index score and alignments deviation. RESULTS AND CONCLUSION:The patients were averagely fol owed up for 29 months. The hospital for special surgery score changed from preoperative 32.5 points to postoperative 87.5 points. The Western Ontario and McMaster Universities Arthritis Index score was increased from preoperative 37.1 points to postoperative 88.8 points (P<0.05). Mean mechanical axis deviation (either varus or valgus) changed from preoperative 17.1° to postoperative 1.4° (P<0.05). Al cases with extra-articular corrective osteotomy healed within 3 months, and none had postoperative infection, loosening or venous thromboembolic events. One patient was with 5° residual knee anti-sheets. Results verified that one-stage total knee arthroplasty procedures are effective in the treatment of knee osteoarthritis accompanied with ipsilateral femoral extra-articular deformity in accordance with individual preoperative plan.
4.Typing,Precaution and Treatment of the Bone Cement Leakage Caused by Percutaneous Vertebroplasty
Yingjie LIU ; Yan DONG ; Weiguang YANG
Journal of Chinese Physician 2001;0(10):-
Objective To explore the typing,precaution and treatment of the bone cement leakage caused by percutaneous vertebroplasty(PVP).Methods PVP was performed in 38 cases(11 men and 27 women) with vertebral angioma and vertebral body compression fracture with osteoporosis chosen from November 2002.Results All the patients were followed up for 8 to 16 months and the effects were evaluated by the standards carried out by World Health Organization(WHO).The rate of complete response(CR) plus partial response(PR) was 100 percent.The patients with bone cement leakage by PVP were divided into 3 groups which underwent different remedial methods respectively.Conclusion The bone cement leakage could be prevented through the preoperative preparation,choosing the anaesthesia and scouting the whole operation.The patients with TypeⅠ,Ⅱ and Ⅲ_a needn't treatment,but those with type Ⅲ_b must accept open-operation.Vertebral eggshell technology with open vertebroplasty was effective on the treatment of the bone cement leakage with type Ⅲ_b caused by PVP.
5.Clinical analysis of 58 cases of amyloidosis
Yingjie MA ; Fengkui LIU ; Yu WANG
Chinese Journal of General Practitioners 2008;7(6):414-415
Fifty eight patients with amyloidosis diagnosed by biopsy were admitted to Beijing Friendship Hospital from January 1983 to September 2007 and their clinical data were analyzed retrospectively.Twenty of the 58 cases were systemic amyloidosis,and other 38 were local amyloidosis.In 20 cased of systemic amyloidosis,kidney was involved in 75% cases,liver was in 55%,nervous system Was in 55%and heart Was in 50%.The lesion of local amyloidosis was usually limited in one single organ.
6.Effect of different filling materials on percutaneous vertebroplasty and the image characteristics
Gang ZHAO ; Hongjian LIU ; Yingjie ZHOU
Chinese Journal of Tissue Engineering Research 2010;14(16):3007-3010
BACKGROUND:Now the filling material which is used for the treatment of osteoporotic compression fractures is mainly polymethacrylic acid (PMMA),but the PMMA as a permanent foreign body cannot promote bone regeneration,and has a downward extension of the possibility of mechanical properties with time prolonging,so a new type of filling material used to substitute PMMA is looking.OBJECTIVE:To investigate the effect and image characteristics of different filling materials on percutaneous vertebroplasty performing on a goat model of osteoporotic compression fracture.METHODS:Three vertebral fracture models were established on osteoporosis goats by using an appropriate mechanical stress.Under the G-arm X-ray machine,the fractured vertebrae was performed with percutaneous vertebroplasty and then filled with polymethyl methacrylate (PMMA),self-setting calcium phosphate cement (CPC) and recombinant human bone morphogenetic protein-2(rhBMP-2)/self-calcium phosphate cement.RESULTS AND CONCLUSION:① A total of 36 vertebral bodies of 12 goats were successfully performed with percutaneous vertebroplasty,and there was no significant difference in filling quantity among three materials (3.5±1.8) mL (P>0.05).Bolus resistance of PMMA was strong,but operative time was short,and the image was great;the bolus resistance of self-CPC and rhBMP-2/self-CPC was weak,operative time was long,but there was no leakage placeholde.②CT scanning results on the 2~(nd) day after surgery demonstrated that the three materials were disorderly distributed in the vertebral body,showing high-density patching shaping.Six out of 36 vertebral bodies were leakage,which was induced by PMMA (n=4),self-CPC (n=1),and rhBMP-2/self-CPC (n=1).③ Scanning electron microscope indicated that all materials in the PMMA group were not able to closely bind with host bone,but self-CPC and rhBMP-2/self-CPC were able to closely bind with host bone.The results suggested that rhBMP-2/self-CPC is characterized by long-term operation,short time of bone substitute,and stabilization of reconstructed spine.
7.The study of the effective mechanism of infected host serum on the infectivity of gametocytes of Plasmodium yoelii (P.yoelii)
Yaming CAO ; Yingjie LIU ; Chao LV ;
Chinese Journal of Immunology 2000;0(08):-
Objective:To clarify the mechanism of crisis serum' mediated gametocyte infectivity to the mosquito vector Methods:Observing the effects of mouse serum , which was obtained 5 days after P yoelii infection (D5 serum) on gametocyte infectivity by IFA and mosquito live feeds, and the production of IFN ??TNF ??IL 4 and NO - 2 in the hosts in vivo and in vitro by ELISA and Griess reaction And to investigate the ability of malaria parasitized red blood cell extract (PRBC extract) to induce NO Results:The development of the gametocytes from mice 5 days postinfection into ookinetes were completely inhibited D5 serum was not immediate to inhibit gametocyte development, which was injected intravenously into the mice 3 days after P yoelii infection But 4 h later after injection D5 serum stimulated the increasing IFN ? and NO production and inhibited gametocyte infectivity Moreover, PRBC extract showed the ability to induce NO Conclusion:Infected host serum blocks transmission of P yoelii via a nitric oxide dependent mechanism
8.Studies on the expression of Pys25 and Pys21 of Plasmodium yoelii and transmission blocking immunity
Yingjie LIU ; Yaming CAO ; Jianzhong YAN ;
Chinese Journal of Immunology 1985;0(02):-
Objective:To investigate the expression of antigens in Plasmodium yoelii sexual stage and transmission blocking effects of monoclonal antibodies(McAbs).Methods:Observing the effects of anti Pys25 McAb4 and anti Pys21 McAb10 on developmental course of parasites in mosquitoes by direct mosquito feeds on passively immunized P.yoelii infected mice and the expression of Pys21 and Pys25 from gametocytes to ookinetes in indicated culture times by IFA and Western blotting.Results:The transmission blocking activity of the anti Pys25 McAb4 was complete and more potent than that of the anti Pys21 McAb10.Both Pys25 and Pys21 were presented in whole developmental course from gametocytes to ookinetes.Furthermore,the expression of Pys25 appeared to be earlier than that of Pys21 on zygote surfact.Conclusion:Pys25 and Pys21 are target antigens of transmission blocking immunity and that anti Pys25 McAb4 has more significantly transmission blocking activity is related with the early stage expression of Pys25 on zygote surface.
9.Using FRET Technique to Investigate The Apoptotic Mechanism Induced by Cisplatin in Living Cells
Lei LIU ; Yingjie ZHANG ; Xianwang WANG
Progress in Biochemistry and Biophysics 2006;0(09):-
Cisplatin, an efficient anticancer agent, can trigger multiple apoptotic pathways in cancer cell. However, the signal transduction pathways in response to cisplatin-based chemotherapy are complicated, and the mechanism is not fully understood. Using fluorescence resonance energy transfer (FRET) technique, the molecular mechanism of cisplatin-induced apoptosis in living human lung adenocarcinoma cells (ASTC-a-1) were investigated. After cisplatin treatment, the recombinant pFRET-Bid and pSCAT-3 probes were used to determine the kinetics of Bid cleavage and Caspase-3 activation, respectively. The fluorescence probes Bid-CFP and DsRed-Mit were also used to detect the spatial and temporal changes of Bid in real-time in sub-cell level. The results showed that a cleavage of the Bid-FRET probe occurring at about 4~5 h after treated with 20 ?mol/L cisplatin. Cleavage of the Bid-FRET probe coincided with a translocation of tBid from the cytosolic to the mitochondria, and the translocation lasted about 1.5 h. At the anaphase of cell apoptosis, Caspase-3 was activated obviously as detected by FRET and Western blotting techniques. Using real-time single-cell analysis, it was observed the kinetics of Bid and Caspase-3 activation for the first time in living cells during cisplatin-induced apoptosis.
10.Treatment of lumbar disc herniation by microendoscopic tubular retractor system (METRx) with a postmedial approach: Report of 290 cases
Yan DONG ; Linjian DING ; Yingjie LIU
Chinese Journal of Minimally Invasive Surgery 2001;0(04):-
Objective To evaluate the therapeutic efficacy of microendoscopic tubular retractor system (METRx) with a postmedial approach in the treatment of lumbar disc herniation. Methods A total of 290 patients with lumbar disc herniation were treated with METRx. The duration of follow-up ranged from 3 months to 33 months. Results According to the Nakai scale, 81% (235 cases) of the patients were evaluated as excellent, 13.4% (39 cases) as good, 4.8% (14cases) as fair and 0.7% (2 cases) as poor. Postoperative infection in intervertebral space was found in 2 cases. Conclusions METRx has a satisfactory short-term therapeutic efficacy in the treatment of lumbar disc herniation.