Purpose　The aim is to purify the peptide with antifu ngal activity from Aspergillus fumigatus culture fluid.Methods　 The pept ide was separated by ion exchange column chromatography and further purified by r everse phase HPLC,and the molecular weight was determined by tricine gel electro phoresis.Results　A kind of peptide(FIP) with antifungal activit y wa s separated. The molecular weight was about 8 000. The detection result by MTT method showed significant antifungal activity of the peptide to 5 kinds of fung i.Conclusion　 The study provided a reliable basis for developin g drug and antiseptic.

The effects of R2O-Al2O3-B2O3-SiO2 system glass and superfine alpha-Al2O3 on the sintering and phase transition of hydroxyapatite (HAP) ceramics were assessed. The results showed that alpha-Al2O3 impeded the sintering of HAP and raised the sintering temperature. When glass and alpha-Al2O3 were used together to reinforce HAP ceramics, better results could be obtained; the bending strength of multiphase HAP ceramics approached 106 MPa when 10% (wt) alpha-Al2O3 and 20%(wt) glass were used and sintered at 1200 for 1 h.
Aluminum Oxide ; chemistry ; Biocompatible Materials ; Boron Compounds ; chemistry ; Ceramics ; chemical synthesis ; chemistry ; Durapatite ; chemical synthesis ; chemistry ; Glass ; chemistry ; Materials Testing ; Silicon Dioxide ; chemistry ; Tensile Strength

HAP-Glass-alpha-Al2O3 gradient coating on alumina ceramics substrate was prepared by multi-layer slurry-dipping and sintering process, effects of the composition of the coating on the microstructure and adhesion strength of the gradient coating were studied. R2O-Al2O3-B2O3-SiO2 system glass with low softening temperature and thermal expansion was used in the gradient coating. The composition of the coating was varied from interface to surface layer, i.e. the HAP concentration increased and glass concentration decreased gradually from the under layer to the surface layer. A suitable amount superfine alpha-Al2O3 was added in the gradient coating to reinforce the bonding strength between the coating and substrate. The results show that the use of R2O-Al2O3-B2O3-SiO2 system glass is propitious to sintering the coating at low temperature and to combination between the coating and substrate. The addition of superfine alpha-Al2O3 can reinforce the bonding strength between the coating and substrate, the highest bonding strength is about 48.2 MPa.
Adhesiveness ; Aluminum Oxide ; chemistry ; Ceramics ; chemistry ; Coated Materials, Biocompatible ; chemistry ; Durapatite ; chemistry ; Glass ; chemistry

OBJECTIVETo discuss the roles of serum interleukin-18 (IL-18), interleukin-10 (IL-10) and soluble interleukin-2R (sIL-2R) in the pathogenesis of chronic hepatitis C and to observe the effects of interferon (IFN) on the above- mentioned serum cytokines.

METHODSThe levels of above- mentioned cytokines were detected in 10 healthy individuals, 24 asymptomatic hepatitis virus C (HCV) carriers and 27 patients with chronic hepatitis C (before and after IFN treatment) using enzyme linked immunosorbent assay (ELISA).

RESULTSThe levels of the cytokines in patients with chronic hepatitis C are higher than in healthy people (P < 0.05) and in asymptomatic HCV carriers (P < 0.05). The values of the cytokines show a significant positive correlation to ALT (P < 0.05). Levels of tested cytokines decreased observably after IFN treatment (P < 0.05). The grades of the serum levels for sIL-2R and IL-10 before IFN treatment (from high to low) were categorized accordingly: non-response group > partial- response group > complete- response group (P < 0.05).

CONCLUSIONSThe tested cytokines co-participate in the pathogenesis of chronic hepatitis C, and can be used to evaluate the effect of IFN on the immune state of organisms. Furthermore, sIL-2R and IL-10 are important for predicting the anti-viral efficacy of IFN.

Adolescent ; Adult ; Antiviral Agents ; therapeutic use ; Female ; Hepatitis C, Chronic ; blood ; drug therapy ; Humans ; Interferon-alpha ; therapeutic use ; Interleukin-10 ; blood ; Interleukin-18 ; blood ; Male ; Middle Aged ; Receptors, Interleukin-2 ; blood ; Recombinant Proteins