1.Status analysis of cervical lesion and cognition of women in childbearing age in Xining and its related factors
Chongqing Medicine 2014;(30):4068-4070
Objective To study the status of cervical lesion and cognition of women in childbearing age in Xining and its related factors .Methods From March 2012 to August 2013 ,1 500 female patients were selected .All the women accepted pap smear and HPV DNA test .Meanwhile ,questionnaires were distributed for cervical cancer in the region and identify the cognition related fac-tors .Results The results showed that among the 1 500 cases of the sample population ,number of cervical intraepithelial neoplasia grade Ⅰ(CIN Ⅰ)was 48(3 .2% )and cervical intraepithelial neoplasia grade Ⅱ and above was 22(1 .5% ) .674 cases got a good cog-nition ,while 826 cases got a poor one .Multivariate analysis showed that age ,marital status ,education level ,place of residence ,occu-pation and family income were closely related to women′s cognition of cervical cancer .Conclusion The status of cervical lesions of women in childbearing age in Xining is generally common ,but the overall cognition is poor ,which is mainly related to age ,marital status ,occupation ,place of residence and income .
3.Performance evaluation and results analysis of chemiluminescence method for detecting plasma BNP
Xiaoming JIANG ; Wenming LIU ; Wen SHI ; Yinghui JIANG ; Jianhua XU
International Journal of Laboratory Medicine 2015;(24):3532-3534
Objective To analyze and evaluate the precision ,accuracy and linearity of the chemiluminescence method for detec‐ting plasma BNP .Methods According to the experimental schemes of CLSI EP15‐A2 ,EP6‐A files and other relevant documents , the precision ,accuracy and linearity of the Siemens Centaur XP chemiluminescence instrument for detecting BNP ,and the detection results were compared with the performance declared by manufacturer or the quality target formulated by laboratory .Results The imprecision of BNP detected by the chemiluminescence method was less than 1/3 TEa regulated by the Clinical Laboratory Center of Ministry of Health (allowable total error);the variation coefficient index (CVR) of internal quality control data was less than ± 2;the relative bias of the results of external quality control blind samples with the target values were less than Tea regulated by the Ministry of Health ;the linear evaluation results showed that BNP was once linearity in the range of 5 .3‐4696 .7 pg/mL .Conclusion The precision ,accuracy and linearity of the Siemens Centaur XP chemiluminescence instrument for detecting BNP can accord withthe quality objectives requirements and meet the clinical needs .
4.Therapeutic effect of NgR siRNA on nerve regeneration following spinal cord hemi-transsection injury in rats
Yinghui BAO ; Jianhong SHEN ; Yumin LIANG ; Qizhong LUO ; Jiyao JIANG
Chinese Journal of Trauma 2008;24(9):696-699
Objective To investigate the therapeutic effect of NSR siRNA on nerve regeneration following spinal cord hemi-transsection injury in rats. Methods Rats with T8 spinal cord hemi-trans-section were didded into 3 groups, ie, siRNA group, NS group and control group. SiRNA or NS was in-jected into lateral cerebral ventricle just after spinal cord injury. The therapeutic effect of NgR siRNA was evaluated by using BBB locomotor rating scale, retrograde horseradish peroxidase(HRP)tracing and HE staining. Results BBB locomotor rating scale showed that the recovery of the locomotor function of siRNA group seemed to be better than that of the other two groups from the 4th week, but there was no statistical difference. Retrograde HRP tracing showed a large number of positive cells in the anterior horn of spinal cord, with statistical difference compared with NS group and control group(P<0. 05). Eight weeks after spinal injury, HE staining showed disorderly distribution of the fibres in NS group and control group but serial fibres in the injury region in siRNA group. Conclusion NSR siRNA may promote the nerve regeneration following spinal cord injury.
5.Correlation analysis of the rheumatoid arthritis and the meteorological factors
Xiaobing YANG ; Feng JIANG ; Yinghui SUN ; Li QIN
Chinese Journal of Rheumatology 2015;19(1):32-35,封3
Objective To explore the relation between rheumatoid arthritis (RA) and the meteorological factors.Methods All RA cases in the group were followed for 12 months,patients were follow-up for every two weeks.In each followed-up,all RA cases were evaluated by the disease activity score-28 (DAS28) and the improved Jamieson questionnaire,and the correlation of the DAS28 and the average values of meteorolo-gical factors (temperature,humidity,atmospheric pressure,relative humidity,wind speed) by Pearson correlation coefficient.The moderate-severe activity cases were evaluated by chi-square test of different seasons.Results One hundred and eighty-eight cases completed the observation,One hundred and sixty-four cases were in moderate disease activities (x2=9.42,P=0.022).Sixty-eight cases were distributed in winter,which was significantly higher than other three seasons.The average temperature and wind speed was negatively related to the DAS28 (r=-0.743,P<0.01; r=-0.576,P=0.003).Conclusion The activity of RA has the characteristics of seasonal changes,and is easily being aggravated in winter.Many factors may influence RA,including temperature and wind speed.Creating an appropriate micro-climate environment is beneficial for RA patients to reduce the disease activity.
6.Microarray analysis of altered gene expression profile of hippocampus after traumatic brain injury in rats
Junfeng FENG ; Kuiming ZHANG ; Xian FU ; Guoyi GAO ; Yinghui BAO ; Yumin LIANG ; Yaohua PAN ; Jiyao JIANG
Chinese Journal of Trauma 2008;24(5):360-366
Objective To screen the altered gene expression profile of hippocampus after traumatic brain injury(TBI)in rats. Methods Rats(n=3)in experimental group underwent moderate fluid-percussion(F-P)brain injury and the hippoeampus sample in the injured hemisphere was removed and conserved in liquid nitrogen three hours later.The rats(n=5)of the control group underwent the same procedure except for injury.Mfymetrix rat genome 230 2.0 array was used to detect the gene expression profile of hippocampus in two groups and find the altered gene expression profile. Results A total of 159 genes in the experimental group changed significantly(≥2 folds)compared with the control group,of which 136 genes were up-regulated and 23 genes down-regulated. Conclusions The significant gene expression changes of hippocampus,especially a large mount of up-regulated genes,are detected after moderate TBI in rats,suggesting that the secondary injury following TBI is a procedure involving multiple factors.
7.Expression changes of microRNA array in mesencephalon of rats with traumatic coma
Guoyi GAO ; Xian FU ; Yumin LIANG ; Yinghui BAO ; Junfeng FENG ; Kuiming ZHANG ; Lei Lü ; Jiyao JIANG
Chinese Journal of Trauma 2008;24(4):274-278
Objective To set up a rat model with acute traumatic coma and identify the variation of microRNA in mesencephalon. Methods After rats were injured moderately by central fluid percussion system, tissues of the mesencephalon were removed one hour after injury. RNA of brain tissue of the mesencephalon was isolated for microRNA array by using the exiqon microarray system. The data were analyzed statistically by Genepix Pro 6.0 after hybridization results were scanned and fluorescence intensity standardized. Resets Hybridization results showed 33 microRNAs with up-regulated expressions but 38 microRNAs with down-regulated activity. Conclusion Expression of microRNA array shows marked changes in the tissues of the mesencephalon in rats with traumatic coma, as may be injury mechanism of traumatic coma and also a way of neurobiological protection of coma.
8.UDP-Ga1NAc: polypeptide α-N-acetygalactosaminyltransferase 2 Localized on Both cis and trans Side of Golgi Stacks in SGC7901 Cells
Yinghui ZHOU ; Saiyu HANG ; Hao QIU ; Wei JIA ; Lan XU ; Zhi JIANG ; Shiliang WU
Progress in Biochemistry and Biophysics 2009;36(1):49-57
Uridine diphosphate (UDP)-GalNAc : polypeptide N-acetylgalactosaminyltransfemse (ppGalNAcT) catalyzes the initial step in mucin type O-glycosylation in the Golgi apparatus. Here generation and characterization of a polyclonal antibody to human ppGalNAcT2 were described. The subcellular location of ppGalNAeT2 in SGC7901 cell line was investigated using Western blot analysis of fractionated cell extracts and confocal microscopy with this antibody and two Golgi markers: Golgi SNARE (soluble N-ethylmalemide-sensifive factor attachment protein receptor) of 28 ku (GS28) and trans-Golgi network (TGN) 38, markers for the c/s- and trans-Golgi apparatus, respectively. Morphometric analyses indicated that ~60% of the ppGalNAcT2 signal colocalized with the GS28, while~36% of the c/s-Golgi marker colocalized with the ppGalNAeT2. Approximately 34% of the ppGalNAcT2 signal colocalized with the TGN38, whereas 38% of the trans-Golgi marker colocalized with the ppGalNAcT2. The results provide unequivocal evidence for the location ofppGalNAcT2 within the Golgi apparatus, and further highlight the importance of this organelle in the initiation of O-linked glycosylation.
9.Experimental study on the effect of RAMP1 -siRNA on CGRP-induced proliferation of MG-63 cells
Zhang JIANG ; Huiyu ZHANG ; Gang ZHANG ; Xiaoqian FENG ; Song SUN ; Yinghui TAN
Journal of Practical Stomatology 2015;(3):339-342
Objective:To investigate the effect of blocking the expression of receptor activity modifying protein 1 (RAMP1 )on calcito-nin gene-related peptide(CGRP)-induced MG-63 cell proliferation.Methods:RAMP1 siRNA was synthesized and screened by tran-scription in vitro.The subcultured MG-63 cells were divided into the following groups:RAMP1 siRNA interference group,empty vector group and blank control group.The mRNA expression and the membrane distribution changes of the calcitonin receptor-like receptor (CRLR)and the receptor component protein (RCP)in MG-63 cells were examined by real-time PCR and immunofluorescence method respectively.Results:RAMP1 and CRLR mRNA and the fluorescence intensity of MG-63 cells decreased after transfection by RAMP1 siRNA(P <0.05).In RAMP1 interference group,the expression of RCP mRNA and the fluorescence intensity were higher than those in the other two groups(P <0.05).After RAMP1 siRNA interference,the proliferation of MG-63 cells was inhibited(P <0.05). Conclusion:RAMP1 siRNA transfection may reduce CRLR expression and inhibite the proliferation of MG-63 cell.
10.Clinical efficacy of early right median nerve electrical stimulation for coma patients followingcraniocerebral trauma
Guoyi GAO ; Yinghui BAO ; Yumin LIANG ; Yaohua PAN ; Shenghao DING ; Jiyao JIANG
Chinese Journal of Trauma 2012;28(3):200-204
ObjectiveTo evaluate the clinical benefits of early right median nerve electrical stimulation on coma patients following craniocerebral trauma. MethodsCraniocerebral trauma patients with up to two weeks of coma in the years 2005-2011 were involved in the study and were randomly divided into control group (received routine management ) and treatment group (routine management plus right median nerve electrical stimulation).The treatment lasted for a period of more than two weeks.The clinical efficacy of the right median nerve electric stimulation and the conscious status of the patients within six months after craniocerebral trauma were observed.ResultsA total of 456 patients were enrolled in the study,of whom 437 patients completed the treatment course,including 221 patients in the treatment group and 216 in the control group.There was no complication related to electric stimulation during the treatment.Cerebral blood flow (CBF) imaging and brain stem evoked potential (BEP) examination demonstrated significant improvement in the treatment group.A total of 386 patients were followed up for six months postoperatively,which showed that there were 122 patients with regained consciousness,46 in minimally conscious state and 36 in vegetative state in the treatment group (204 patients) and there were 84 patients with regained consciousness,40 in minimally conscious state and 58 in vegetative state in the control group ( 182 patients).The patients in the treatment group showed a higher ratio of regained consciousness and a lower ratio of vegetative state compared with the control group,but the ratio of minimally conscious state showed no statistical difference between two groups. ConclusionsRight median nerve electrical stimulation is a suitable coma awaking means at early stage after craniocerebral trauma.