Objective To study the efficacy of alendronate in treatment of postoperative residual pain with osteoporotic vertebral compression fractures(OVCF) of vertebroplasty.Methods 62 patients with OVCF from September 2013 to October 2014 were divided into observation group and control group, each had 31 cases.All patients were given calcium carbonate D3 tablets 1 day after surgery, observation group were given alendronate on this basis.The degree of pain score between two groups pre-and post-treatment two weeks, one month, two months were observed and compared.Pain improvement in two groups were observed, and activity ability score, painkiller use and bone mineral density between two groups pre-and post-treatment were compared.Results The pain scores in observation group a month postoperative was (0.8 ±0.4) points and (0.4 ±0.2) points after two months, were significantly lower than those in control group[(1.6 ±0.7) points, (1.1 ±0.8) points, P<0.05].The excellent rate of pain improvement in observation group(83.87%) was significantly higher than that in control group (58.06%, P <0.05) .After treatment, activity ability score in observation group was (1.13 ±0.53) points and painkiller usage was (0.44 ±0.07) times per day, all significantly lower than those in control group [(1.79 ±0.89) points, (0.89 ±0.12) times per day, P<0.05].BMD level in observation group was (5.78 ±1.02)%, higher than that in control group[(3.21 ±0.79)%, P<0.05].Conclusion Alendronate in treatment of osteoporotic vertebral compression fractures vertebroplasty postoperative residual pain has good early outcome and low adverse reactions, and security is relatively high.

AIM: To establish the capillary GC method for separating and determining both camphor and menthol in Compound Dexamethasone Acetate Cream. METHODS: The methyl salicylate was used as internal substance and the sample solution was prepared by extracting with anhydrous ethanol at(60 ?C.) The GC test conditions consisted of Agilent HP-INNOWAX(30 m?0.320 mm?0.25 ?m) as the stationary phase,nitrogen as the carrier gas,split ratio of 25.0(∶)1.The flow rate was 1.5 mL/min,column temperature was at 145?C,inlet temperature was(200 ?C),FID detection was(250 ?C),injection volume was 0.6 ?L. RESULTS: The linearities of camphor and menthol were good,in the range of 0.303-3.030 g/L with r=0.999 9 for camphor and in the range (0.306)-(3.060) g/L with r=0.999 9 for menthol.The average recoveries of high,middle and low three concentrations for menthol were 100.6%,100.2%, 99.7%;RSD was 0.58%,0.65%,0.88% and for camphor were 100.3%,(99.8%),(97.8%);RSD was 0.43%,0.78%,1.0%. CONCLUSION: This method is convenient,rapid,accurate and reproducible,and can be used for quality control of Compound Dexamethasone Acetate Cream.

Objective:To separate and purify the immunoactive polysaccharide RTPS- I from Trichosanthis Radix and study the physicochemical properties and preliminary structure. Methods:The crude polysaccharides were extracted by water, and then precipita-ted by ethanol. After the deproteinization with trichloroacetic acid, the polysaccharide RTPS-I was separated and purified by using DE-AE-Cellulose, DEAE-Sepharose FF and Sephadex G-200 column chromatography. The analysis of properties and structure was per-formed by using GC, HPGPC, IR and NMR. Results:RTPS-I was a white flocculent solid after vacuum freeze-drying, and soluble in water. It contained three elements (carbon, hydrogen and oxygen) with the specific rotation [α]25D (H2O) of +164. 44 and the relative molecular mass of 17555. The glycosyl in RTPS- I was composed of glucose without uronic acid. The sugar ring was pyranoid ring, and each monosaccharide was linked by α-glycosidic bond. Conclusion: A homogeneous immunoactive polysaccharide RTPS- I from Trichosanthis Radix is isolated for the first time.

OBJECTIVE:To establish a method for the determination of residual solvent of ethanol and toluene in diphenhydr-amine hydrochloride raw material. METHODS:Headspace capillary gas charmatography and butanone as internal standard were used. The column was Agilent DB-624 capillary column,inlet temperature was 200 ℃,hydrogen flame ionization detector was 250 ℃,the carried gas was high purity nitrogen,flow rate was 3.0 ml/min with temperature programmed,the splitting-ratio was 20∶1,the containers of headspace injector were in equilibrium at 80 ℃ for 30 min,and the injection time was 1 min. RESULTS:With this chromatographic condition,ethanol,toluene and internal standard peak were well separated;there was a good linear rela-tionship of ethanol and toluene in the range of 0.02-0.8 mg/ml (r=0.999 8 and r=0.999 4);RSDs of precision and stability test were lower than 3%;recoveries were 95.50%-103.50%(RSD=2.6%,n=9) and 96.91%-103.74%(RSD=2.2%,n=9). CON-CLUSIONS:The method is simple,sensitive and accurate,and can be used for the determination of residual solvent of ethanol and toluene in diphenhydramine hydrochloride raw material.

Objective To establish a RP-HPLC method for determination of ammonium glycyrrhizinate in Shufei Mixture .Methods The sample was analyzed on an Agilent Zorbax SB-C18 column (4.6mm ×150 mm, 5μm), and acetonitrile-0.0025 mol/L sodium heptanesulfonate solution–0.05 mol/L potassium dihydrogen phosphate solution(20:45:45) ( adjusted pH value of 7.2 ±0.05 with 20% sodium hydroxide solution) was used as mobile phase.The flow rate was at 1.0 mL/min.The detective wavelength was at 250 nm.The column temperature was 30 ℃.Results With this chromatographic condition, the ammonium glycyrrhizinate peak in Shufei Mixture sample chromatogram could be separated with other ingredient peaks completely.The negative sample had no interference.The calibration curve was linear at a ranges of 23.6-118.1μg/mL for ammonium glycyrrhizinate, and equation of regression was Y=0.1133X–0.00110,r=0.999 8.The average recovery from sample was 97.8% and RSD was 0.88%(n=6).The content range of ammonium glycyrrhizinate in three batch Shufei Mixture sample was 0.2532-0.2865 mg/mL, and average content was 0.2721 mg/mL. Conclusion This method is simple, accurate, and useful for control method of this preparation.

Objective To improve the quality of aiye processed products, an eucalyptol content in commercially available aiye two processed products of chao aiye and aiye tan was investigated.Methods A capillary gas chromatography was used.The sample was prepared with n-hexane by reflux condensation.Chromatographic conditions: The separation was carried on an Ailgent DB-1 capillary column(30 mm ×0.320 mm ×0.25 μm). Inlet temperature was 200℃ and FID temperature was 250℃.The programmed column temperature was set as follows:maintained at 100℃ for 6 min and raised to 160℃ at the rate of 20℃/min followed by holding for 3min.The splitting-ratio was 5.0:1.The carried gas was nitrogen, flow rate was 1.0 mL/min.Injection volume was 1μL.Results In the given chromatographic conditions, the eucalyptol chromatographic separation had good, and the separation degree was greater than 1.5 between eucalyptol and other impurity peak.The linear range of eucalyptol was 11.4-114.0 mg/mL(r=0.999 5). Methods repeatability and recovery were good.The minimum limit of quantification was 0.5μg/mL.The results of determination of eucalyptol show that the eucalyptol content in the commercially available 11 batch of chao aiye was between 5.6-78.2 μg /g, and 12 batch of aiye tan had no eucalyptol. Conclusion The processing technology of current commercially available aiye processed products of chao aiye and aiye tan need to be improved, and the quality standard need to be improved.

Since 2010,the activities of high quality nursing service demonstration project were carried out in the national health system,its main purpose is to strengthen the nursing foundation,and provide satisfactory service.By conducting these activities,significant achievements have been made,such as improved nursing quality,reduced nurse-patient disputes.However,in the process of implementation,there are stillsome problems for us to think about and solve.In this paper,there is a brief commentary about the results of high quality nursing implementation and related issues.

Objective:To optimize the ethanol extraction technology for Xiaoluo granules. Methods:The extraction technology for the ethanol dissolvable ingredients in 10 kinds of medicinal materials was optimized by orthogonal experiment. The weighted sum of ba-icalin content and the dry extract rate as the index, 4 influencing factors with 3 levels each were optimized by L9 (34 ) orthogonal table. Results:The optimal ethanol extraction conditions were as follows:the medicinal material was soaked by 10-fold 50% ethanol solution for 45min, and then extracted by heating reflux for 3 times with 1. 5h each time. Conclusion: The method can effectively extract the ethanol dissolvable ingredients in 10 kinds of medicinal materials, which can be used as the ethanol extraction technology for Xiaoluo granules.

Objective To investigate the changes and significance of serum C reactive protein (CRP) and calcitonin (PCT) levels in the elderly patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD).Methods One hundred and twenty elderly patients with AECOPD in the respiration deplartment of our hospital from January 2013 to study One hundred and twenty elderly patients with AECOPD in our hospital from January 2013 to June 2015 were selected to conduct the study and performed the subgroup analysis according to the BODE index (body weight,dyspnea,airflow,motor function index),times of acute exacerbation within 1 year after treatment and recurrence time,and other 40 healthy subjects in the outpatient department were selected as the control group.The serum PCT and CRP levels were compared among various groups.Results Serum CRP and PCT levels in AECOPD patients with grade 1-4 of BODE index were significantly higher than those in the control group (P＜0.05),moreover in the intra-group comparison of serum CRP and PCT,the grade l＜grade 2＜grade 3＜grade 4,the differences were statistically significant (P＜0.05).Serum CRP and PCT had the significantly positive correlation with the grades of BODE index in AECOPD patients (r=0.482,0.317,P＜0.05).After treatment,serum CRP and PCT levels in AECOPD patients with recurrence occurred more than 3 months were significantly lower than those in the patients with recurrence occurred within 3 months,the difference was statistically significant (P＜0.05);serum CRP and PCT levels in the patients with more than once recurrence at 1 year after discharge from hospital were significantly higher than those in the patients with recurrence ≤once,the difference were statistically significant (P＜0.05).Conclusion The serum levels of CRP and PCT in the patients with AECOPD can reflect dyspnea,airflow limitation and motor function ability,and conducts the preliminary assessment on the patient's prognosis.

Objective To optimize ethonal extraction technology for no sugar runfei granules.Methods Ethanol was used as a solvent to extract the ethanol dissolvable ingredients in 9 kinds of medicinal materials.The formula L9 (34 ) table was used to examine the effects of 4 factors and 3 levels.The weighted sum of baicalin content and the dried extract quantity was used as quantitative index.Results The maximize optimize condition for extraction of ethanol dissolvable ingredients was as follows:9 kinds of medicinal materials, add of 10-fold 70% ethanol solution, soaked for 45 min, and extracted by heating reflux for 3 times,1.5 h each time.Conclusion The method can maximize extraction of ethanol dissolvable effective ingredents in 9 kinds of medicinal materials and can be used as ethonal extraction technologgary of no sugar runfei granules.