Abnormal auditory responses is one of the common features in autism. The characteristics and pathogenesis of the abnormality is not quite clear. The results of electrophysiological hearing evaluation ( i. e,brainstem auditory evoked potentials, otoacoustic emissions) in autistic children are inconsistent. The abnormal aduitory responses may contribute to the poor social interaction and communication in autism.

Objectlve To investigate recurrent respiratory tract infection in children with T-cell subsets and related eytokines change.Methods Choose meet the RRTI diagnostic criteria for children and healthy infants,application APAAP double antibody ELISA method and the sandwich method ty detect RRTI other healthy infants and children with the T-lymphocyte subsets and cytokines interferon γ(IFN-γ),interleuldn 10(IL-IO),interleukin 6 (IL-6)levels,and compared,Results Children with recurrent respiratory tract infection of T lymphocyte subsets CD+3,CD+4decreased co;increased serum IL-6(2.5±1.6 ku/L),IL-10(3.1±2.4 ku/L)and IFN-γ(12.6±6.4μg/L)levels were significantly below the healthy group(6.8±4.7 ku/L、7.4±3.5 ku/L、21.5±9.7μg/L)(P＜0.01).Conclusion The boay of the RRTI children humoral and cellular immune existed varying degrees of functional defects.

Objective: we investigated the biological effects of exogenous S100A6 on osteosarcoma cell line U2OS and explored its possible mechanism. Methods:MTT assay was used to determine S100A6 influence on U2OS cells proliferation and their conce ntration-dependent relationship. The trypan blue exclusion assay was used to observe S100A6 inhibitory effect on U2OS cells proliferation in a time-dependent manner;Hoechst staining was used to determine cell apoptosis;Western blot and immunocytochemical methods to determine expressions of cyto-?-catenin. Results:We found that ,on the first three days,OD values in cells subjected to the recombinant protein S100A6(GST-S100A6)with the concentrations of 30,100,300 and 1 000?g/ml respectively were obviously lower than those in the GST group,by 20.5%,23.7%,32.2% and 36.8% accordingly(P

A femail patient was presented to our department for a dark red painless mass in the left auricle, which has progressively enlarged for one year. Pathological examination revealed granuloma change. Mycobacterium tuberculosis DNA test was positive. Tuberculin test revealed strong positive reaction. This case was diagnosed as tuberculous granuloma.
DNA, Bacterial ; isolation & purification ; Ear Auricle ; microbiology ; pathology ; Female ; Granuloma ; microbiology ; pathology ; Humans ; Mycobacterium tuberculosis ; isolation & purification ; Tuberculosis ; pathology

Objective:To construct secreted chimeric vector of AFP-HSP70 and investigate transient expression of vectors in eukaryotic cells.Methods:Vectors were constructed by routine molecular technique.Fusion genes were linked with G-S-G-G-S linker.Vectors were transfected into COS-7 cells by Lipofectamine 2000. Proteins were assayed by immunocytochemistry and RT-PCR for its RNA.Results:Proteins expressed in COS-7 were comfirmed by immunocytochemistry 48 hours later and RNA was detected by RT-PCR.Conclusion:AFP-HSP70 chimeric vector is constructed successfully and could be expressed in eukaryotic cells.

Objective To investigate the effects of pentoxifylline (PTX) on the expression of uncoupling protein 2 (UCP-2) of hepatic mitochondria in rats with nonalcoholic fatty liver disease (NAFLD). Methods Sixty SD rats were randomly divided into 3 groups (each n = 20): normal control group, experiment group and treatment group. The rats in normal control group were given a normal feed. The rats in experiment and treatment groups were given fat-rich feed. Furthermore, the rats in treatment group were given PTX after 4 weeks in fat-rich diet feeding. The expression of UCP-2 in the liver was detected by immunohistochemistry and semi-quantitative RT-PCR. Results The hepatic expression of UCP2 mRNA was higher in experiment group (4.0±0.3) than in normal control group (1.2±0.1). The hepatic expression of UCP2 mRNA was higher in treatment group (3.0±0.2) than in normal control group, but the hepatic expression of UCP2 mRNA was lower in treatment group than in experiment group (F = 160. 67, P＜ 0. 01). Conclusions The UCP-2 mRNA is expressed in livers of NAFLD, pentoxifylline plays an important role in the reduction of expression of UCP-2 mRNA in lives of NAFLD.

Objective To evaluate the change of left ventricular(LV) function by real-time three dimensional echocardiography(RT-3DE) and to explore the clinical value of change of serum adiponectin level in patients with metabolic syndrome (MS).Methods One hundred and twenty patients were divided into MS group and control group according to the diagnostic standard of the nternational Diabetes Federation.Transthorasic 2D ultrasound and RT-3DE examination were performed in all study objects, and serum adiponectin levels were measured.Results Parameter index [including Tmsv16-SD, Tmsv12-SD,Tmsv16- Dif, Tmsv12-Dif, Tmsv16-SD (％), Tmsv 12-SD (％), Tmsv16-Dif (％), Tmsv12-Dif (％)] of 17segment time to minimal systolic volume in MS group was more than that in control group (all P ＜0.05).Some regional systolic index of LV and serum adiponectin level were less than that in control group (all P ＜0.05).Compared with the control group, E/E' was lower (P ＜ 0.05).There were significant relations between serum adiponectin level and E/A of mitral valves, E'/A' of mitral annulus and E/E'(r = 0.368,0.493,and -0.347 respectively, P ＜0.05).Conclusions RT-3DE can rapidly evaluate LV systolic and diastolic synchrony in MS patients.E/E' show an excellent correlation with serum adiponectin level,and it may be a new promising method for routine evaluation of LV diastolic function.

AIM: To investigate the adsorption mechanism of pueraria on the molecular imprinting polywer(MIP) and Scatchard analysis was carried out. METHODS: Pueraria MIP was synthesized using pueraria as the template,acrylamide as the functional monomer and ethylene glycol dimethaerylate(EDMA) as the crosslinker. RESULTS: The result showed that at least two classes of binding sites were formed in the imprinted polymer.Extracting compounds from Radix Pueraria was separated by MIP. CONCLUSION: The component of the products is analyzed by HPLC.The result shows that the final products contain pueraria and the other two kinds of isoflavone.

Three problems about the concept of validity were discussed here,which is the evolution of concept of validity and its existing problems,a new understanding of validity and how to construct validity.During the development of the concept of validity,content validity,predictive validity,construct validity as well as concurrent validity were prevailing until the emergence of construct validity in which all aspects of validity were involved.To date,the bias we put on the concept of validity were mainly originated from the way by which we have taken concept of validity as a nomological network.Actually,the concept of validity is a ontological claim which is seriously based on reference and causality.We should establish a theory hypothesis,form a test,and confirm the measure attribute,so we could obtain the validity.

Objective To observe clinical effect of treating chronic renal faihire(CRF) with herbal enema by high buttock positon. Methods 100 patients with CRF were randomly re, cuffed into a treatment group and a control group. The treatment group was treated with herbal enema by high buttock positon, and the control group was treated with activated carbon (Ai'xite), which were taken orally. Results The total effective rate in the treatment group and the control group was 88.6% and 50.0% respectively. Conclusion The therapeutic effect of treating CRF with herbal enema by high buttock position was better.