1.Fingerprint of Flos Lonicerae by HPLC-FPC
Qian WANG ; Jianxin WANG ; Zhiguo YU ; Xin HU ; Yinghu ZHANG
Chinese Traditional Patent Medicine 1992;0(07):-
AIM To establish the HPLC-FPC of Flos Lonicerae . from different growing areas. METHODS The gradient was adopted elution with methanol and 1 % acetic acid, at detection wavelength was at 254 nm. RESULTS The constitutents of Flos Lonicerae were well separated on HPLC, and the HPLC-FPC of 10 batchs of samples was established. The results showed that this method had a good repeatability, and the 11 common peak’s had an exclusive properties. CONCLUSION The method developed can evaluate the quality of Flos Lonicerae conveniently.
2.Clinical effect of ablation in treatment of needle tract implantation after radiofrequency ablation for primary liver cancer
Ningning LU ; Haiyan WANG ; Yinghu ZHANG
Journal of Clinical Hepatology 2019;35(4):813-817
ObjectiveTo investigate the risk factors for needle tract implantation after radiofrequency ablation for primary liver cancer and the clinical effect of ablation. MethodsA retrospective analysis was performed for the clinical data of 4 patients with needle tract implantation after radiofrequency ablation for primary liver cancer who underwent ablation therapy in Center of Interventional Oncology and Liver Diseases, Beijing YouAn Hospital, from January 2017 to October 2018. Risk factors, the clinical effect of ablation, and complications were analyzed. ResultsAll four patients had intrahepatic tumor lesions located near the Glisson′s capsule, and among these patients, two had poorly differentiated hepatocellular carcinoma (HCC), one had moderately differentiated HCC, and one had well-differentiated HCC. The time from radiofrequency ablation to needle tract implantation ranged from 3.6 to 14.3 months, and all four patients had a single lesion of needle tract implantation, which was located at the anterior abdominal wall in three patients and at the right abdominal wall in one patient. Of all patients, three underwent argon-helium cryoablation, and one underwent radiofrequency ablation. Two patients experienced pyrexia and transient aggravation of pain after surgery, and the symptoms were relieved after symptomatic treatment. No serious complication was observed. Contrast-enhanced computed tomography performed at one month after surgery showed complete ablation of metastatic lesion in three patients, with no recurrence after follow-up for 2-19 months. One patient was found to have local residual lesion, with marked relief of pain, and no enlargement of the residual lesion was observed after follow-up for 3 months. All patients had significant reductions in serum alpha-fetoprotein and abnormal prothrombin at one month after surgery. ConclusionAblation therapy, especially argon-helium cryoablation, has a good clinical effect in the treatment of needle tract implantation at the abdominal wall, with the advantages of few complications and good tolerability. Therefore, it is a good choice for patients who cannot tolerate surgical operation or are unwilling to undergo surgical operation.
3. Report of antimicrobial resistance surveillance program in Chinese children in 2016
Chuanqing WANG ; Aimin WANG ; Hui YU ; Hongmei XU ; Chunmei JING ; Jikui DENG ; Ruizhen ZHAO ; Chunzhen HUA ; Yinghu CHEN ; Xuejun CHEN ; Ting ZHANG ; Hong ZHANG ; Yiping CHEN ; Jinghong YANG ; Aiwei LIN ; Shifu WANG ; Qing CAO ; Xing WANG ; Huiling DENG ; Sancheng CAO ; Jianhua HE ; Wei GAO ; Shuzhen HAN
Chinese Journal of Pediatrics 2018;56(1):29-33
Objective:
To analyze the antimicrobial resistance profile in Chinese children.
Methods:
This was a prevalence survey. From January 1 through December 31, 2016, the isolates were collected from 10 tertiary children hospitals in China. Antimicrobial susceptibility testing was carried out by routine laboratory methods. The penicillin susceptibility of
4. Clinical characteristics and antimicrobial resistance of pneumococcal infections from 9 children's hospitals in 2016
Chao FANG ; Xuejun CHEN ; Mingming ZHOU ; Yinghu CHEN ; Ruizhen ZHAO ; Jikui DENG ; Chunmei JING ; Hongmei XU ; Jinhong YANG ; Yiping CHEN ; Hong ZHANG ; Ting ZHANG ; Sancheng CAO ; Huiling DENG ; Chuanqing WANG ; Aimin WANG ; Hui YU ; Shifu WANG ; Aiwei LIN ; Xing WANG ; Qing CAO
Chinese Journal of Pediatrics 2018;56(8):582-586
Objective:
To describe the clinical characteristics of pneumococcal infections and drug resistance of
5.Expert consensus on standardized TORCH laboratory detection and clinical application
Yuning ZHU ; Shiqiang SHANG ; Yinghu CHEN ; Dapeng CHEN ; Liting JIA ; Wei QU ; Jiangwei KE ; Haibo LI ; Xiaoqin LI ; Xiuyun LIANG ; Yanqiu LIU ; Lijuan MA ; Liya MO ; Qiang RUAN ; Guosong SHEN ; Yuxin WANG ; Hong XU ; Jin XU ; Liangpu XU ; Xiaohong XU ; Enwu YUAN ; Lehai ZHANG ; Wenli ZHANG ; Xinwen ZHANG
Chinese Journal of Laboratory Medicine 2020;43(5):553-561
TORCH, which is considered as a series of pathogens, including the Toxoplasma gondii, Rubella virus, Cytomegalovirus or Herpes simplex virus, often infects the pregnant women to induce the the fetus or newborn infection by transplacental infection or exposure to contaminated genital tract secretions at delivery. Increasing evidence have been confirmed that the infection of TORCH may cause the miscarriage, premature birth, malformed fetus, stillbirth, intrauterine growth retardation, neonatal multiple organ dysfunction and other adverse pregnancy outcomes. For most TORCH-infections cases may lacking the effective treatments during pregnancy, and it is important to achieve the effacing monitoring of TORCH infections before and during pregnancy. The laboratory testing of TORCH has the great significance. However, the consensus opinions still need to improve the the standardization of TORCH testing process and the correct interpretation. Based on the characteristics of the TORCH detection method, this article gives a consensus opinion on the standardized detection and clinical application of TORCH from the laboratory perspective according to the characteristics and types of infection of different pathogens.
6.Distribution characteristics and drug resistance of carbapenem-resistant Enterobacteriaceae in chil-dren from 2016 to 2017
Bingjie WANG ; Fen PAN ; Hong ZHANG ; Ting ZHANG ; Hongmei XU ; Chunmei JING ; Chuanqing WANG ; Aimin WANG ; Hui YU ; Shuzhen HAN ; Aiwei LIN ; Shifu WANG ; Qing CAO ; Xing WANG ; Chunzhen HUA ; Yinghu CHEN ; Xuejun CHEN ; Jikui DENG ; Ruizhen ZHAO ; Huiling DENG ; Sancheng CAO ; Jianhua HAO ; Wei GAO ; Yiping CHEN ; Jinhong YANG
Chinese Journal of Microbiology and Immunology 2019;39(8):583-590
Objective To investigate the distribution and drug resistance of carbapenem-resistant Enterobacteriaceae ( CRE) isolated from children in China. Methods CRE strains were collected in 10 ter-tiary children's hospitals of China from January 1, 2016 to December 31, 2017. Antimicrobial susceptibility of the clinical strains was detected with disk diffusion method ( KB method) and automated method. The re-sults were analyzed according to the Clinical and Laboratory Standards Institute ( CLSI) Standards published in 2017. WHONET 5. 6 software was used to retrospectively analyze the distribution characteristics and drug resistance of these strains. Results A total of 3065 CRE clinical strains were isolated from children with an overall prevalence of 7. 7% and among them, 13. 5% were isolated in neonatal group and 5. 8% in non-neo-natal group. The detection rate of CRE in 2017 was higher than that in 2016 (9. 7% vs 5. 7%). Among the 3065 CRE strains, there were 1912 strains of Klebsiella pneumoniae (62. 0%), 667 strains of Escherichia coli (22. 0%), 206 strains of Enterobacter cloacae (7. 0%), 56 strains of Klebsiella aerogenes (1. 8%) and 47 strains of Serratia marcescens (1. 5%). Most of the strains were isolate in neonatology departments including neonatal intensive care units (NICU) and intensive care units (ICU), accounting for 44. 8% and 19. 7%, respectively. Respiratory tract (61. 8%), urine (19. 4%) and blood (5. 7%) specimens were the main sources of CRE isolates. Results of antimicrobial susceptibility test showed that the CRE strains were highly resistant to carbapenem antibiotics such as imipenem, meropenem and ertapenem, as well as penicillins and most cephalosporins (79. 6%-100%), especially those isolated in the neonatal group (P<0. 05). Children had relatively low resistance rates to aminoglycosides such as amikacin (19. 7%) and fos-fomycin (11. 9%), fluoroquinolones such as levofloxacin (37. 7%) and ciprofloxacin (43. 3%), and tige-cycline (3. 8%). Currently, no polymyxin B-resistant strains were isolated. Conclusions The prevalence of common CRE strains in children in 2017 was higher than that in 2016, especially in newborns. Drug re-sistance in CRE strains isolated from neonates to common antibiotics was more severe, suggesting that great attention should be paid to it and timely measures should also be taken.
7.Applicability of Foot-Ground Contact Model at Different Gait Speed
Yinghu PENG ; Zhenxian CHEN ; Jiayu HU ; Zhifeng ZHANG ; Zhongmin JIN ; Pingping WEI
Journal of Medical Biomechanics 2019;34(5):E514-E521
Objective To establish the musculoskeletal multi-body dynamic foot-ground contact model and explore its applicability at different speed. Methods The gait data of the subjects at different speed were collected, and the foot-ground contact model was established based on the full body model from the musculoskeletal multibody dynamic software AnyBody. Then the calculated ground reaction forces (GRFs) and ground reaction moments (GRMs) at different speed (slow walking, normal walking, fast walking and jogging) were compared with the measurements from the force plates. Results The predicted GRFs and GRMs correlated well with the experimental measurements at slow, normal and fast speed (stride speed ranged from 0.69 to 1.68 m/s). The correlation coefficients between predicted and measured GRFs were greater than 0.875 and the correlation coefficients for GRMs were greater than 0.9. Conclusions The developed foot-ground contact model could simultaneously predict GRFs and GRMs with good accuracy, thus eliminating the dependency on force plates. The model could be applied to low-speed gait conditions, such as the elderly and pathological gait.
8.Early antiviral therapy of abidol combined with lopinavir/ritonavir and recombinant interferon α-2b for patients with COVID-19 in Zhejiang: A multicenter prospective study
Runan WEI ; Nanhong ZHENG ; Xiangao JIANG ; Chunlian MA ; Xiaowei XU ; Shourong LIU ; Yongping CHEN ; Kaijin XU ; Hainv GAO ; Jiansheng ZHU ; Qiang SHU ; Jifang SHENG ; Xiaoqiang ZHANG ; Minghui LI ; Yan ZHANG ; Mengjie MA ; Xuan ZHANG ; Shibo LI ; Qiujing WANG ; Lingjun YING ; Yongjun ZHANG ; Yunzhen SHI ; Lingyan FAN ; Wanjun YU ; Huaying WANG ; Dandan SUN ; Xiaodong WANG ; Jichan SHI ; Yinghu CHEN ; Xinsheng XIE ; Yunqing CHEN ; Weihong WANG ; Zhaowei TONG ; Lingling TANG ; Mengfei ZHU ; Lingjian ZHANG ; Lanjuan LI
Chinese Journal of Clinical Infectious Diseases 2020;13(1):9-15
Objective:To compare the efficacy of the combination of abidol, lopinavir/ritonavir plus recombinant interferon α-2b (rIFNα-2b) and the combination of lopinavir/ritonavir plus rIFNα-2b for patients with COVID-19 in Zhejiang province.Methods:A multicenter prospective study was carried out to compare the efficacy of triple combination antiviral therapy and dual combination antiviral therapy in 15 medical institutions of Zhejiang province during January 22 to February 16, 2020. All patients were treated with rIFNα-2b (5 million U, 2 times/d) aerosol inhalation, in addition 196 patients were treated with abidol (200 mg, 3 times/d) + lopinavir/ritonavir (2 tablets, 1 time/12 h) (triple combination group) and 41 patients were treated with lopinavir/ritonavir (2 tablets, 1 time/12 h) (dual combination group). The patients who received triple combination antiviral therapy were further divided into three subgroups: <48 h, 3-5 d and >5 d according the time from the symptom onset to medication starting. The therapeutic efficacy was compared between triple combination group and dual combination group, and compared among 3 subgroups of patients receiving triple combination antiviral therapy. SPSS 17.0 software was used to analyze the data.Results:The virus nucleic acid-negative conversion time in respiratory tract specimens was (12.2±4.7) d in the triple combination group, which was shorter than that in the dual combination group [(15.0±5.0) d] ( t=6.159, P<0.01). The length of hospital stay in the triple combination group [12.0 (9.0, 17.0) d] was also shorter than that in the dual combination group [15.0 (10.0, 18.0) d] ( H=2.073, P<0.05). Compared with the antiviral treatment which was started within after the symptom onset of in the triple combination group, the time from the symptom onset to the viral negative conversion was 13.0 (10.0, 17.0), 17.0 (13.0, 22.0) and 21.0 (18.0, 24.0) d in subgroups of 48 h, 3-5 d and >5 d, respectively ( Z=32.983, P<0.01), while the time from antiviral therapy to viral negative conversion was (11.8±3.9), (13.5±5.1) and (11.2±4.3) d, respectively( Z=6.722, P<0.05). Conclusions:The triple combination antiviral therapy of abidol, lopinavir/litonavir and rIFNα-2b shows shorter viral shedding time and shorter hospitalization time, compared with the dual combination antiviral therapy; and the earlier starting triple combination antiviral therapy will result in better antiviral efficacy.
9. A multicentric clinical study on clinical characteristics and drug sensitivity of children with pneumococcal meningitis in China
Caiyun WANG ; Hongmei XU ; Jikui DENG ; Hui YU ; Yiping CHEN ; Aiwei LIN ; Qing CAO ; Jianhua HAO ; Ting ZHANG ; Huiling DENG ; Yinghu CHEN
Chinese Journal of Pediatrics 2019;57(5):355-362
Objective:
To understand clinical characteristics of children with
10.Diagnosis and treatment recommendation for pediatric COVID-19 (the second edition).
Zhimin CHEN ; Junfen FU ; Qiang SHU ; Wei WANG ; Yinghu CHEN ; Chunzhen HUA ; Fubang LI ; Ru LIN ; Lanfang TANG ; Tianlin WANG ; Yingshuo WANG ; Weize XU ; Zihao YANG ; Sheng YE ; Tianming YUAN ; Chenmei ZHANG ; Yuanyuan ZHANG
Journal of Zhejiang University. Medical sciences 2020;49(2):139-146
The coronavirus disease 2019 (COVID-19) has caused a global pandemic. All people including children are generally susceptible to COVID-19, but the condition is relatively mild for children. The diagnosis of COVID-19 is largely based on the epidemiological evidence and clinical manifestations, and confirmed by positive detection of virus nucleic acid in respiratory samples. The main symptoms of COVID-19 in children are fever and cough; the total number of white blood cell count is usually normal or decreased; the chest imaging is characterized by interstitial pneumonia, which is similar to other respiratory virus infections and infections. Early identification, early isolation, early diagnosis and early treatment are important for clinical management. The treatment of mild or moderate type of child COVID-19 is mainly symptomatic. For severe and critical ill cases, the oxygen therapy, antiviral drugs, antibacterial drugs, glucocorticoids, mechanical ventilation or even extracorporeal membrane oxygenation (ECMO) may be adopted, and the treatment plan should be adjusted timely through multi-disciplinary cooperation.
Betacoronavirus
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isolation & purification
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Child
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Coronavirus Infections
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diagnosis
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pathology
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therapy
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Humans
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Pandemics
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Pneumonia, Viral
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diagnosis
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diagnostic imaging
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etiology
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pathology
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therapy