Objective To explore the effect of repetitive transcranial magnetic stimulation (rTMS) on motor function in cerebral palsy children with epilepsy. Methods 270 cerebral palsy children with epilepsy hospitalized from February, 2011 to September, 2014 were ran-domized to control group (n=130) and treatment group (n=140). The control group received comprehensive rehabilitation, including mas-sage, exercise, occupational therapy, physiotherapy, speech training, cognitive function training and medication, and the treatment group re-ceived rTMS in addition. They were assessed with Gross Motor Function Measure-88 (GMFM-88) and Peabody Developmental Measure Scale-Gross Motor (PDMS-GM) before, and 1 and 2 months after treatment. Results The total scores of GMFM-88 and PDMS-GM in-creased more in the treatment group than in the control group (F>93.9, P<0.001). Conclusion rTMS can further improve the motor function in cerebral palsy children with epilepsy.

Objective To investigate the effects of specific immunotheraphy(SIT) using allergens for children atopic dermatitis(AD) to find an efficacious method to treat AD. Methods 148 children with AD were divided into SIT group and control group. All patients were given allergen intradermal tests. The patients in SIT group received SIT using positive aero-allergens for 8 to 18 months. The patients in control group received the treatment of antihistamine drugs and drugs for external use. Results Total effective rate and curative rate of SIT group were 93.2% and 68.9% respectively, and those of control group were 68.9% and 17.6% respectively, which had a significant difference between the two groups(P

Objective To investigate the MRI manifestations of cerebral glioma involving corpus callosum,and to enhance the ability to diagnosis and differential diagnosis.Methods Thirty four cases of cerebral glioma involving corpus callosum under went plain and Gd DTPA enhanced MR scans.The MRi findings of these gliomas were compared with operation and pathology.Results Gerebral gliomas involving corpus callosum presented as long T 1 and long T 2 homogeneous or inhomogeneous signal intensity and obvious mass effect.The MRI findings of Gd DTPA enhanced MR scans were obvious enhancement,slight enhancement or no enhancement accouding to different pathological kinds of gliomas."Butterfly signs" and "semi butterfly signs" were the reflections when gliomas of corpus callosum invaded two sides or one side of cerebral hemisphere.These two signs were significant markings in the diagnosis of cerebral gliomas in corpus callosum region.Conclusions Cerebral gliomas involving corpus callosum are intracranial tumors with special location.MRI has significant clinical value in the diagnosis and differential diagnosis of this kinds of tumour.

Surgical resection is the treatment of choice for patients with colorectal liver metastases.The criteria for hepatic resection of colorectal liver metastases have been extended.Patients who were expected to have complete tumor resection,negative resection margin and adequate remnant liver volume could receive surgical resection.Surgical resection combined with local ablation can expand the resectability of liver metastases.There is no significant difference of long-term survival between simultaneous and staged resection for colorectal and liver tumors.Laparoscopic surgery has been proved as safe and feasible for colorectal liver metastases.The survival benefits of adjuvant chemotherapy during perioperative period is unclear,and neo-adjuvant chemotherapy might not benefit all the patients with colorectal liver metastases.

Objective To investigate an effective algorithm for image segmentation in cervical cancer cell adhesion , which enables accurate segmentation of the contour of adherent cells .Methods The images of target cells were extracted from the background area using level set methods , normalized with minimum values of transformation algorithms ,and multi-plied by the gradient image points in the region of interest ( ROI) to inhibit the undesired gradient information before the im-ages of adherent cells were segmented using labeled watershed algorithm .Results and Conclusion Compared to conven-tional watershed segmentation methods , this algorithm is not only effective in image segmentation of adherent cervical cancer cells with uneven staining and more accurate segmentation lines established around the contours of adherent cells , but of high clinical value .

AIM To study the effects of A? 25～35 and Apo E4 on neuronal intracellular free Ca 2+ ([Ca 2+ ] i). METHODS Hippocampal and cortical neurons suspension of newborn(0～3 days) SD rats was produced. After incubated with fura 2/AM,the neurons suspension was divided into four groups: control, A? 25～35 , Apo E4, A? 25～35 +Apo E4. Each groups [Ca 2+ ] i was measured using a RF 5000 dual wavelength spectrofluorometer after incubated with double distilled water, A? 25～35 , Apo E4, A? 25～35 +Apo E4 for 3 min, respectively. The neurons outocorrelation function(ACF) of the scattering light intersity was analyzed by the microscope quasi elastic light scattering(MQLS) technique The frequency shift line width by ACF. The ? can sympolize the cell menbrane flilidity. RESULTS Both A? 25～35 and Apo E4 could significantly enhance hippocampal and cortical neurons rest [Ca 2+ ] i, furthermore, the effect of 5 ?mol?L -1 A? 25～35 was higher than the effect of 1 ?mol?L -1 A? 25～35 ( P

Objective To explore the dose and effects of mixtures of pethidine and haloperidol for Postoperative Deliri-um. Methods Retrospective analysis was conducted on treatment dose and effects of 85 cases of patients with deliri-um during narcotic awake period or 1 day to 2 days after operation by mixtures (haloperidol with pethidine) from 2005 to 2006 in Affiliated Hospital of Hainan Medical College. Results 1/4 dose of mixture (pethidine 100 mg and haloperidol 5 mg) cured 9 patients, 1/2 dose of mixture cured 61 cases, we add 1/4 dose of mixture when 10 cases occurred delirium again ,5 cases added 1/2 dose of mixture again, all were cured. After administration ,aublood pres-sure declined all, but all were within normal range, the 17 cases's pulse oxygen saturation is less than 95%, Conclu-sion The mixture (haloperidol with pethidine) can be effective treatment for delirium during awake period of general anesthesia or after operation. However, application of low dose therapy from the beginning, and attention to its impact on respiration.

Aim Toinvestigatetheeffectofhigh,me-dium and low doses of Mahuang decoction on the re-lease amount of rat hippocampal neural transmitter (Glu,Gly,Asp,GABA),then compare Mahuang de-contion with ephedra alkaloids and ephedra.Methods Ratswererandomlydividedinto6groupsandgiven orally with Mahuang decoction of high dose (calculated by ephedra 4 g·kg-1 ),medium dose (calculated by ephedra 2 g·kg-1 ),low dose (calculated by ephedra 1 g · kg-1 ),ephedra (calculated by ephedra 2 g · kg-1 ),ephedra alkaloids (ephedrine 7 mg · kg-1 , pseudoephedrine 2. 4 mg · kg-1 , methylephedrine 1. 12 mg · kg-1 )and blank control group.Samples were obtained from the hippocampus of conscious rat by microdialysis sampling technique.The content of ami-no acid neurotransmitters in dialysates was detected u-sing the established HPLC-ECD with OPA pre-column derivationmethod.Results Fouraminoacidneuro-transmitters could be well separated in 28 min.High, medium and low doses of Mahuang decoction,ephedra and ephedra alkaloids significantly increased the con-tent of these four amino acid neurotransmitters,com-pared with blank control group (P<0. 05 ).Ephedra alkaloids significantly reduced the levels of inhibitoryamino acid neurotransmitters GABA and Gly in 90 min,compared with the medium dose of Mahuang de-coction.Excitatory neurotransmitters of ASP and Glu in hippocampus showed the trend of increase first and then decrease after oral administration of Mahuang de-coction and ephedra. The levels of Glu and Asp reached peaks from 90 min to 120 min after treatment with Mahuang decoction,and also increased along with dose increase of Mahuang decoction.In comparison with the medium dose of Mahuang decoction group,the level of Glu reached peak at 90 min and 150 min in ephedra alkaloids group and ephedra group respective-ly,and the content of Glu significantly increased at peaktime.Conclusions Increasedcontentofexcita-tory amino acid neurotransmitters (Asp and Glu ) shows positive correlation with the dose of Mahuang de-coction.Other components in Mahuang decoction in-hibits the up-regulation effect of ephedra and ephedra alkaloids on Glu,and promotes the up-regulation effect of ephedra alkaloids on GABA and Gly.

AIM　To study the effects of Aβ25～35 and Apo E4 on neuronal intracellular free Ca2+(［Ca2+］i). METHODS　Hippocampal and cortical neurons suspension of newborn(0～3 days) SD rats was produced. After incubated with fura-2/AM,the neurons suspension was divided into four groups: control, Aβ25～35, Apo E4, Aβ25～35+Apo E4. Each groups ［Ca2+］i was measured using a RF-5000 dual wavelength spectrofluorometer after incubated with double distilled water, Aβ25～35, Apo E4, Aβ25～35+Apo E4 for 3 min, respectively. The neurons outocorrelation function(ACF) of the scattering light intersity was analyzed by the microscope quasi-elastic light scattering(MQLS) technique The frequency shift line width by ACF. The Γ can sympolize the cell menbrane flilidity. RESULTS　Both Aβ25～35 and Apo E4 could significantly enhance hippocampal and cortical neurons rest ［Ca2+］i, furthermore, the effect of 5 μmol*L-1 Aβ25～35 was higher than the effect of 1 μmol*L-1 Aβ25～35 (P<0.05), and they also amplified KCl-induced rise in ［Ca2+］i in hippocampal and cortical neurons(P<0.05). The interaction of Aβ25～35 and Apo E4 could also significantly enhance hippocampal and cortical neurons rest ［Ca2+］i andamplified KCl-induced rise in ［Ca2+］i in hippocampal and cortical neurons(P<0.05), but they had no synergic or additive effect.The frequency shift line widith Γ of both hippocampal and cortical neurons were decreased by both Aβ25-35 and ApoE4. CONCLUSION　Aβ25～35 and Apo E4 could enhance neuronal intracellular free Ca2+, amd decrease meirpma; ,e,brame f;iodotu. But their interaction had no synergic or additive effect. It suggested that the amplified effect of Aβ25～35 and Apo E4 on neuronal ［Ca2+］i and membane fluidity may be relative to their neurotoxity.

Objective To analyze lesional and clinical characteristics of Langerhans cell histiocytosis in children.Methods A clinical retrospective study was performed on 126 patients with Langerhans cell histiocytosis collected from 2006 to 2011 at the Hunan Children's Hospital.Results Of the 126 patients,the youngest was 2months old,and the oldest was 9 years old.The ratio of male to female was 2.5 ∶ 1.Clinical manifestations included eczematid,seborrheic lesions,hemorrhagic maculopapules,yellow nodules and white macules.Of the three clinical phenotypes of Langerhans cell histiocytosis,Letter-Siwe disease was the most prevalent,and most cases of LetterSiwe disease were associated with hepatosplenomegaly,abnormal chest X-ray,impaired hematopoietic function and multifocal bone injuries.The clinical grade was mainly Ⅲ and Ⅳ in patients with Letter-Siwe disease,Ⅰ in patients with eosinophilic granuloma,and varied from Ⅰ to Ⅳ in patients with Hand-Schuller-Christian disease with Ⅱ as the most common.Of these patients,those with eosinophilic granuloma had the oldest average age with bone as the only affected organ,while those with Letter-Siwe disease had the youngest average age with the greatest number of affected organs.The treatment of Langerhans cell histiocytosis included surgical operation and combined chemotherapy.Conclusions Langerhans cell histiocytosis has characteristic skin lesions and diverse clinical manifestations.Pathology has diagnostic significance to Langerhans cell histiocytosis.Therapy strategies and curative effects are dependent on the severity of,and the organs affected by Langerhans cell histiocytosis.