Objective To evaluate the effects of exog en ous adenovirus-mediated gene transfer of human tumor suppressor gene PTEN (phos phatase and tensin homology deleted on chromosome 10) on cell prolifiration and invasive ability of human prostate cancer cell lines LNCaP and DU-145. Methods mRNA and protein expressions of PTEN in human prostate c ancer LNCaP and DU-145 cells infected with AdPTEN were detected by RT-PCR and Western blot.MTT assay was used to assess the effect of PTEN on cell proliferati on.The change of cell cycle and apoptosis were measured by flow cytometry and th e invasion abilities in vitro detected by Boyden chamber.Ad-X-TRE-?gal v irus was used as control. Results RT-PCR and Western bl ot analysis showed that LNCaP and DU-145 cells express high level of PTEN after its infected with Ad-PTEN. The growth of LNCaP and DU-145 cells treated with Ad-PTEN was significantly more inhibited than that with a control adenovirus.Ad -PTEN-infected LNCaP and DU-145 cells showed a significant increase in G 0～ G 1 phase and PTEN-induced apoptosis in comparison with the control.The invasiv e ability of prostate cancer cell lines was inhibited by expression of PTEN effe ctively. Conclusions Adenoviral-mediated expression of human tumor suppressor gene PTEN has inhibitory affect on proliferation and inva sive ability of human prostate cancer cell lines LNCaP and DU-145.This study pr ovides an idea for the further development of Ad-PTEN as a potential therapeuti c vector in the gene therapy of prostate cancer.

Objective　To evaluate the status of urological laparoendoscopic single-site surgery (LESS) technique in China.　Methods　We had conducted a systematic review of literatures of urological laparoendoscopic single-site surgery published from Chinese institutions using WanFang,CNKI,VIP,CBM,GoogleScholar and MEDLINE databases.All relevant articles were selected with consensus and analyzed according to the inclusion and exclusion criteria.　Results　Overall,205 articles from 66 different centers all over China published between January 2009 and June 2012 were included in the analysis. Of which,there were 26 articles of science citation index papers and 179 articles from Chinese academic journals.The included literature was mainly composed of case series reports ( 133 articles,64.9％ ),comparison studies (13 articles,6.3％ ),reviews or comments (20 papers,9.8％ ),nursing related papers (28 pieces,13.7％ ),animal surgery studies (6 articles,2.9％ ),and training (5 articles,2.4％ ).A total of 1790 clinical cases were reported during the study period,56.9％ of these were procedures done in the upper urinary tract,only 5.8％ were in the lower urinary tract,and 37.3％ were genital or other procedures.Procedures of tumor-related and reconstruction required accounted for 18.8％ and 18.7％,respectively.Pure single-port technique was applied on 58.3％ of cases.In cases of a single-port platform used,45.6％ used the commercially available devices and 54.4％ used homemade devices.Transperitoneal accesses were adopted in 59.5％ of cases and the other 40.5％ were performed through extraperitoneal approaches.The umbilicus was used as the site of access in 58.5％ of cases.A total of 25 indications were reported,including adrenalectomy,nephrectomy and ureterolithotomy,etc. in the upper urinary tract,radical cystectomy,radical prostatectomy and transvesical enucleation of the prostate,etc.in the lower urinary tract,and varicocelectomy,orchidopexy and others. The three most often done procedures were varicocelectomy (601　cases,33.6％ ),renal cyst decortication (408 cases,22.8％ ) and adrenalectomy ( 179 cases,10.0％ ).The overall conversion rate was 5.5％,and complications were encountered in 3.4％ of cases.　Conclusions Laparoendoscopic single-site surgery has been widely adopted by Chinese urologists and largely covered the spectrum of conventional urologic laparoscopic procedures,with most of these being non-reconstructive operations for the treatment of benign diseases.In large,we are still under the initial stage of LESS application in urology.And more work on creative innovation and well-designed studies are needed to further evaluate its role in the urological minimally invasive surgery.

Lower urinary tract symptoms (LUTS),consisting storage,voiding and postmicturition symptoms,is a comprehensive definition involving multiple organs.There has been an increasing emphasis on the integrated management of non-neurogenic male lower urinary tract symptoms.Instead of focusing on the enlarged prostate,the current treatment has paid more attention on the entire urinary tract as well as multiple organ factors.Therefore,we provided a literature review and summarized the key points during the management of male LUTS as 3B,namely beyond prostate,beyond surgery and beyond urology.

Laparoscopic partial nephrectomy is gaining more and more popularity in treating patients with early renal carcinoma in recent years.Compared with radical nephrectomy,laparoscopic partial nephrectomy is a mini-invasive,safe method with satisfactory postoperative survival rate.However, there were still limited laboratory and clinical data about laparoscopic partial nephrectomy,and the experimental data of traditional laparoscopic surgery and open surgery were still used.Based on the existing data and the authors' experience,this article proposes three hypotheses for the problems puzzling urologic surgeons;the authors hope to verify the hypotheses through basic or clinical research.

38.5 ℃) between two groups showed no significant difference(8.3% vs 6.9%,?2=0.000,P=1.000).Conclusions As to skilled urologists in conditioned hospitals,PCNL can be considered to be the first choice for impacted upper ureteric calculi.

0.05).The urethral catheter was indwelled for a mean of 2.5 days(2-4 days).Follow-up examinations were conducted for 5-24 months(mean,17 months).At the first postoperative month,the IPSS and maximum urinary flow were 6.7?2.4 and 16.3?6.1 ml/s,respectively,both of which were significantly improved as compared with preoperative values(t=8.975 and 7.325,P

Objective To explore indications and safety of tubeless percutaneous nephrolithotomy(PCNL).MethodsPatients were enrolled into this study at the end of PCNL according to their pre-and intra-operative conditions.The inclusion criteria included:the degree of nephrohydrosis by preoperative B-ultrasonography less than 4 cm;preoperative level of serum creatinine is normal;single percutaneous tract;no sign of urinary infection before and during operation;no obvious operative bleeding or severe collecting system perforation;no residual stone or with small residual stone but no need for second percutaneous operation;operation time for PNCL less than 2 hours(double-J tube placement was placed successfully).A total of 240 patients with upper ureteral calculi or renal stones were selected and randomly allocated to no nephrostomy drainage(Group A,120 patients) or standard(F14) nephrostomy drainage(Group B,120 patients) groups according to the parity of the patients' number. Results PCNL was completed successfully in both the groups with a high rate of stone clearance [98.3%(118/120) vs 96.7%(116/120),?2=0.171,P=0.679].No patient required blood transfusion.After the operation,18 patients showed fever but without infectious shock(Group A: 8 vs Group B: 10,?2=0.240,P=0.624).No perinephric fluidity was observed by B-ultrasonography either in Group A or Group B in 48 hours after the surgery.Conclusions As for strictly selected patients,tubeless PCNL is a safe and effective treatment.

Proprotein convertase subtilisin/kexin type 9 (PCSK9) plays an important role in lipid reg-ulation through interaction with low-density lipoprotein cholesterol receptors , and several types of PCSK 9 inhibi-tors are gradually becoming research hotspots due to their lipid lowering effect .Among them PCSK9 monoclonal antibodies are the closest to clinical application , and a number of phase Ⅲclinical trials in PCSK9 monoclonal antibodies have been completed in recent years .We systemically reviewed the current clinical research on PC-SK9 monoclonal antibodies in this paper , in order to understand their efficacy and long-term safety in reducing the risk of cardiovascular diseases .

Objective To probe into the clinical and urodynamic features of primary bladder neck obstruction(PBNO) in females. Methods A retrospective analysis was made with comprehensive urodynamic evaluation in 61 cases with PBNO and 32 cases with genuine stress incontinence. Results According to the modified phentolamine UPP tests,there were 41 cases of internal sphincter spasticity(ISS)and 20 cases of bladder neck obstruction(BNO).The success rate of P Q plot analysis in the ISS group was lower than those of BNO and the control(49%vs 80% and 81%).94% of both ISS and BNO patients had a Lin PURR grading of ≥grade Ⅱ,whereas 99% of the control group were of grade 0 of I.There were a significant difference in such parameters as PVB,A G number(Pdet Qmax -2Qmax),post voiding residual,and maximum urethral closure pressure between the obstructed groups and nonobstructed group. Conclusions The meaningful diagnostic parameters for PBNO are Lin PURR grading(≥grade Ⅱ),PVB and A G number.The modified phentolamine UPP test is a reliable index for the assessment of internal sphincter sphincter functional obstruction.

Objective To study the mechanism of occurrence of the multifocal bladder cancers. Methods The expressions of p21,p53 and EMA in twenty specimens from 5 cases of multifocal bladder cancers were studied by means of immunohistochemistry. Results In 4 of 5 cases, the expressions of p21 and p53 were correspondent in scattering specimens from the same patient. In all the 5 cases, the expressions of EMA were correspondent in scattering specimens from the same patient. Conclusions Multifocal bladder cancer may originate from the same monocolone.