Objective To study the effect of skin stratum corneum on transdermal absorption characteristic of Sinomenine Liposome Patch. Methods The normal and stripped stratum corneum skin of back and abdomen was used to study the transdermal absorption characteristic by Franz method. The transdermal absorption sample was taken at the preset sampling time point. The sample content was determined by HPLC. The cumulative transdermal amount of per unit area was fitted with sampling time point, the transdermal rate constant (J value) was selected as comparative standard. Results The transdermal rate constant (J) of skin with stratum corneum stripped increased obviously than that of the normal skin, so was the released rate constant. Conclusion The skin stratum corneum has an notable impact on transdermal absorption characteristic of Sinomenine Liposome Patch. When the skin of experimental animal being prepared, the integrality should be paid more attention.

Objective To investigate the effects of edaravone on myocardial fibrosis induced by isoproterenol (ISO) in rats, and to discuss the correlation between the level of transforming growth factor-β1 (TGF-β1) and the myocardial fibrosis. Methods Forty male SD rats were randomly divided into five groups, namely control group, model group and edaravone groups (low, medium and high doses). Isoproterenol was used to establish the rat model of myocardial fibrosis. Edaravone groups were given edaravone [3, 5 and 10 mg/(kg · d)] to intervene for 14 days. The activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) were examined after 15-d treatment. The left ventricular mass index (LVMI) and collagen volume fraction (CVF) were examined. The expression of TGF-β1 was detected by Western blot assay and immuno-fluorescence method. Results The content of MDA and LVMI were significantly higher in model group than those of the control group (P<0.01),whereas the content of SOD was significantly lower in model group than that of the control group (P<0.01). Compared with model group, the expression level of MDA decreased with the increased intervention dose of edara-vone (P<0.05), while SOD expression level increased (P<0.05). There was no significant difference in the level of SOD be-tween middle dose edaravone group and the control group. LVMI was decreased with the increased doses of edaravone ( P<0.01). There was no significant difference in LVMI between the high dose of edaravone group and the control group. Com-pared with the control group, the expression level of TGF-β1 was significantly increased in model group (P<0.01). The ex-pression level of TGF-β1 was reduced with the increased doses of edaravone. CVF was significantly increased in model group compared with that of control group (P<0.001). CVF decreased with the increased doses of edaravone in medium and high doses of edaravone groups, but they were higher than that of control group (P<0.01). TGF-β1 was positively correlated with MDA, LVMI and CVF (r=0.931, 0.879 and 0.930, P<0.001). SOD was negatively correlated with TGF-β1 (r=-0.892, P<0.001). Conclusion Edaravone can relieve myocardial fibrosis by inhibiting oxidative stress and TGF-β1 in rats.

Objective To explore the effects of nicorandil on cardiac function and clinical outcomes in patients with acute myocardial infarction (AMI) undergoing percutaneous coronary intervention (PCI). Method Sixty-six patients with AMI were randomized into a control group and nicorandil group (n = 33 for each group). In the nicorandil group, nicorandil (4 mg as a bolus injection followed by constant infusion at 8 mg/hour for 24 hours) was administered immediately after admission. Reactive oxygen species (ROS) formation was assessed by measuring urinary excretion of 8-epi-prostaglandin F2α (PGF2α) and compared between the two groups; cardiac function and cardiac events were also compared. Results Urinary 8-epi-PGF2αexcretion was increased 2-fold at 60 to 90 minutes after PCI in the control group, whereas it was unchanged in the nicorandil group (P < 0.001). Left ventricular ejection fraction and cardiac index immediately after PCI and at 6 months were greater in the nicorandil group than in the control group(P < 0.05). Rates of total inhospital cardiac events and rehospitalization were lower in the nicorandil group than in the control group (P<0.05). Conclusions Nicorandil improves cardiac function and clinical outcomes in patients with AMI undergoing primary percutaneous coronary intervention. Suppression of ROS formation may be involved in the potential mechanism.

We retrospectively analyzed 218 allergen skin prick tests ( SPT ) of the patients with chronic sinusitis with or without nasal polyps( CRSwNP/CRSsNP) before the operation. The proportions of positive SPT in CRSwNP and CRSsNP were compared respectively. The distributions of allergens were the same in all groups. The positive rate of SPT in CRSwNP was higher than that in CRSsNP, with statistical significance(P<0. 05). The positive rate of SPT in CRSsNP was higher than that of the control group, without statistical significance. The recurrence rate in CRSwNP was higher than that in CRSsNP, with statistical significance. The recurrence rate in the allergen-positive patients was higher than the allergen-negative patients, with statistical significance.

Objective To evaluate the safety and efficacy of Centurion Vision System for subhixated cataract surgery.Methods Clinical data of 15 eyes with lens subluxation treated with Centurion Vision System were retrospectively reviewed.Ocular variables including visual acuity,intraocular pressure,corneal endothelial cell counts (CEC),central foveal retinal thickness (CFRT),intraocular lens (IOL) position,as well as intraoperative and postoperative complications were observed.Results The best corrected visual acuity (BCVA) was 0.6 or better in 10 eyes (66.7％) 3 months after surgery,while the BCVA of the other 5 eyes was between 0.2 and 0.6 mainly due to pre-existing macular degeneration caused by high myopia or trauma.CEC and CFRT values demonstrated a statistically significant difference between the preoperative and all postoperative follow-ups (all P ＜ 0.05).Three months after surgery,no serious surgeryrelated complications,such as retinal detachment,IOL dislocation,suprachoroidal hemorrhage,macular edema,were observed.Conclusion Centurion Vision System can facilitate phacoemulsification in eyes with subluxated lenses,and this technique is safe and effective due to providing excellent outcomes and avoiding vitreoretinal complications.

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[ABSTRACT]OBJECTIVETo explore the basic characters of the airflow-field in Chinese people's nasal cavity by computational fluid dynamics.METHODSThe three-dimensional, finite-element mesh were developed from Spiral CT imaging scans of nose of the 40 healthy Chinese people. Given the following spatial boundaries of the flow field: no-slip condition was imposed at the surface of the nasal airway walls; a standard atmosphere pressure condition was established at the inlet; a velocity vector was specified at the outlet (nasopharynx), which was obtained under the condition of aspiratory flow rate (12 L/min), the full Navier-Stokes and continuity equations were solved to obtain the airflow pattern.RESULTS1. The airflow passed mainly through left or right side of the nasal airway in the whole 40 cases (left 33, right 7),and the volume of air through the main-side is (320±28) ml while non-main-side (180±45) ml. 2. Airflow velocity: airflow of anterior nostrils, internal nostrils, the middle and inferior parts of the total meatus in the main-side were (5.01±2.12) m/s, (7.00±1.75) m/s, (5.08±1.55) m/s, (4.12±1.40) m/s respectively, and those in non-main-side were (2.01±0.94) m/s, (2.40±0.34) m/s, (1.99±1.0) m/s, (2.01±0.65) m/s respectively, which differences between the both sides were of statistical significance (allP<0.05); airflow of middle meatus, inferior meatus and olfactory cleft in the main-side were (2.08±0.43) m/s, (1.60±0.95) m/s, (1.64±0.80)m/s respectively, and those in non-main-side were (1.89±0.52) m/s, (1.49±0.89) m/s, (1.50±0.75) m/s respectively, which differences were significant statistically (allP>0.05); 3. The airflow form appeared to be linear in the middle and inferior parts of the nasal cavity. 4. Velocity in maxillary sinus cavity was almost 0 m/s.CONCLUSIONThe airflow passes mainly through the middle and inferior parts of the meatus with higher velocity in laminar form and airflow of middle meatus, inferior meatus and olfactory cleft are low and the velocity were slow. Besides, airflow in maxillary sinus cavity diffuses free mainly.

Objective Screening the potential lncRNAs of KIAA1456 inhibiting ovarian cancer cells.MethodsImmunofluorescence was used to detect the expression of KIAA1456 in HO8910PM ovarian cancer cell.Microarray was performed to identify differently expressed lncRNAs in HO8910PM ovarian cancer cells overexpressed KIAA1456 as compared with the normal control group. The interesting candidate lncRNAs were verified by real-time quantitative PCR.Constructing RNAi lentiviral vector targeting KIAA1456 gene in HO8910PM ovarian cancer cells, RT-qPCR and Western blot was performed to measure the interference efficiency.Differentially expressed lncRNAs were validated by RT-qPCR after KIAA1456 knock-down.Results KIAA1456 mainly expressed in nucleus of HO8910PM cell.A total of 654 differently expressed lncRNAs were identified in the KIAA1456-overexpressed group compared with the control group with a set filter fold-change ≥1.2, the most aberrantly expressed lncRNAsincluding down-regulated gene SSX8 and up-regulated genes SNORD14D,SNORA26,lncRNA-ENST00000384488.The results of the RT-qPCR were consistent with the data from the microarray.The shRNA interference vectors targeting the KIAA1456 gene were successfully constructed, SSX8 was up-regulated, and SNORD14D,SNORA26,lncRNA-ENST00000384488 were down-regulated after knockdown the expression of KIAA1456 in the HO8910/PM ovarian cancer cells.Conclusions KIAA1456 inhibits proliferation of ovarian cancer by regulating correlative lncRNAs, which may provide a new target for the diagnosis and treatment of ovarian cancer.

Objective: To investigate of e-cigarette use among middle school students in Hainan Province, so as to provide insights into tobacco control among adolescents. Methods: Students were randomly sampled using a multistage stratified cluster random sampling method from three junior high schools, two high schools and one vocational high school in Hainan Province from July to October, 2021. Participants' basic features, use of e-cigarettes, e-cigarette advertising exposure were collected using the Questionnaire on Tobacco and Alcohol Prevalence among Chinese Adolescents in 2021 prepared by Chinese Center for Disease Control and Prevention. Following data weighting, students' use of e-cigarettes and exposure to e-cigarette advertisements were descriptively analyzed, and factors affecting current use of e-cigarettes were identified using a multivariable logistic regression model. Results: A total of 8 561 questionnaires were allocated, and 8 538 valid questionnaires were recovered, with an effective recovery rate of 99.73%. The respondents included 4 470 boys (52.35%) and 4 068 girls (47.65%), and there 4 367 junior high school students (51.15%), 3 482 high school students (40.78%), and 689 vocational high school students (8.07%). The rate of e-cigarette use was 25.01% and the rate of current e-cigarette use was 4.68%. There were 2 728 respondents with exposure to e-cigarette advertising during the past 30 days, and the number of weighted individuals were 210 932 (32.60%). The route of e-cigarette advertising exposure mainly included point-of-sale (21.59%), stores (13.61%), and shopping websites (9.01%). Multivariable logistic regression analysis identified region (OR=0.610, 95%CI: 0.482-0.772), males (OR=1.332, 95%CI: 1.062-1.669), high school students (OR=0.376, 95%CI: 0.259-0.545), companion smoking (OR=6.645, 95%CI: 4.935-8.948) and advertising exposure (OR=3.229, 95%CI: 2.581-4.040) as factors affecting current use of e-cigarettes. Conclusions The use of e-cigarettes among middle school students is higher in Hainan Province than the national level, and exposure to e-cigarette advertisements may facilitate use of e-cigarettes. It is necessary to strengthen the supervision of e-cigarettes, strictly restrict e-cigarettes advertisements and promotions.