OBJECTIVEThe genetic difference among Scrophularia ningpoensis cultivars were analyzed in molecular level.

METHODNinety-two individuls of three S. ningpoensis cultivars were employed to be analyzed by the approach of Sequence-related Amplified Polymorphism (SRAP).The parameters were calculated by POPGENE1.31 and the relationship was constructed based on UPGMA method.

RESULT1) A total of 227 bands were scored and 199 bands of them were polymorphic. 2) The result is showed that there is a medium level of genetic diversity among three cultivars. At species level: percentage of polymorphic loci PPB=52.42%, effective number of alleles N(e)=1.2812, Nei's gene diversity H=0.1671 and Shannon's information index H(sp)= 0.2526; At cultivar level: PPB=21.44%, N(e)=1.1216, Nei's gene diversity H=0.0725 and Shannon's information index H(pop)= 0.1083. 3) The Nei's coefficient of genetic differentiation was 0.5625, which was consistent with the Shannon's coefficient of genetic differentiation (0.5713). Most of the genetic variation existed among cultivars. 4) The gene flow (N(m)=0.3889) was less among cultivars, indicating that the degree of genetic differentiation was higher. 5) Genetic similarity coefficient were changed from 0.8082 to 0.9133. By clustering analysis, the classified result of SRAP marker between traditional modal character was almost same.

CONCLUSIONThe genetic diversity of samples of S. ningpoensis is medium. The genetic difference among cultivar is higher than that within cultivar.

Cluster Analysis ; DNA Primers ; genetics ; Gene Flow ; Genetic Markers ; genetics ; Genetic Variation ; Nucleic Acid Amplification Techniques ; methods ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Scrophularia ; classification ; genetics ; Software

OBJECTIVETo study the genetic diversity of Codonopsis tangshen.

METHODEighteen germplasmic resources of C. tangshen were analyzed by SRAP and ISSR molecular markers. The systematic diagram of genetic relationship was made by TREECONW software and clustered by UPGMA method.

RESULTTwenty-nine SRAP primer combination amplified 329 bands with 266 (80.85%) polymorphic and 21 ISSR primers amplified 223 bands with 166 (74.44%) polymorphic. The average genetic similarity coefficient was 0.7121 (by SRAPs) and 0.7781 (by ISSRs). Both SRAP and ISSR analyses revealed a high level of genetic diversity in C. tangshen. By cluster analysis, the geographical distribution was not distinctive. The significant positive correlation between SRAPs and ISSRs was observed (r=0.802, P<0.01), although the dendrograms based on SRAP and ISSR markers were not all the same.

CONCLUSIONDifferent germplasms diversity of C. tangshen is high and SRAP and ISSR can be used in genetic diversity study of C. tangshen.

Algorithms ; Codonopsis ; genetics ; DNA, Plant ; analysis ; Genetic Markers ; genetics ; Genetic Variation ; Genotype ; Phylogeny ; Polymorphism, Genetic ; Sequence Homology, Nucleic Acid

Objective To investigate the correlation between the uncertainty in illness and coping style among cervical cancer patients .Methods A total of 180 patients with cervical cancer were selected and investigated with Mishel ’ s uncertainty in illness scale-adult form ( MUIS-A ) and medical coping modes questionnaire ( MCMQ) .Pearson correlation analysis was used to analyze the correlation between uncertainty in illness and coping style.Results The MUIS-A total score of patients with cervical cancer was (78.91 ±9.34). The score of MCMQ facing factor, avoiding factor and yielding factor was (19.28 ±2.61), (17.56 ±2.12) and (12.90 ±1.43), respectively.The MUIS-A total score of patients with cervical cancer was positively correlated with the yielding factor (P<0.01), and negatively correlated with the facing and avoiding factors (P<0.05).Conclusions Correlation has been found between uncertainty in illness and coping style in patients with cervical cancer .Nurses should guide patients to adopt effective coping styles , in order to decrease their uncertainty in illness and improve their quality of life .

Objective:To explore the application value of Kegel exercise combined with moxibustion acupuncture point therapy in preventing and treating bladder dysfunction of patients with cervical neoplasms.Methods:A total of 180 patients who underwent radical hysterectomy of cervical neoplasms in Department of Gynecologic Oncology in Tumor Hospital Affiliated to Guangxi Medical University from December 2017 to April 2019 were selected as research objects by the convenience sampling method. They were randomly divided into 3 groups, with 60 cases in every group. Patients in the control group were given routine nursing, the observation group one was given Kegel exercise training on the basis of the control group and the observation group two was given Kegel exercise combined with moxibustion acupuncture point therapy on basis of the control group. The incidence of urinary retention, bladder function recovery and postoperative quality of life after intervention were compared among the three groups.Results:There were statistically significant differences in indwelling time, residual urine volume, and urinary retention rate among the three groups of patients ( P<0.05) . The differences in postoperative bladder function among the three groups were statistically significant ( P<0.05) . The proportion of patients with bladder function ofⅢ-Ⅳ in the observation group 2 was lower than those in the control group and the observation group 1. In the comparison of Postoperative Quality of Life Scale scores, the differences between the scores of physiological scores, functional scores, cervical cancer subscales and the total Postoperative Quality of Life Scale scores of the three groups were statistically significant ( P<0.05) . Conclusions:Kegel exercise combined with moxibustion acupuncture point therapy can effectively reduce the incidence of postoperative urinary retention in patients with cervical neoplasms, promote the recovery of bladder function and improve the quality of life of patients after surgery.

ObjectiveTo explore the possible mechanism of action of Bushen Huoxue Granules （补肾活血颗粒， BHG） in the treatment of Parkinson's disease （PD） through the Nrf2/NLRP3 inflammasome axis. MethodsA total of 84 male C57/BL 6 mice were randomly divided into blank group， model group， Madopar group， dimethyl fumarate group， and low-， medium， and high-dose BHG group， with 12 mice in each group. Except for the blank group， all groups were induced into PD models by intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine （MPTP） at a concentration of 30 mg/ml for 7 consecutive days. The blank group received an equal volume of saline. After model establishment， the low-， medium， and high-dose BHG groups were treated with 1.5， 3， and 6 g/（kg·d） of the BHG by gavage， respectively. The Madopar group was given 0.113 g/（kg·d） of Madopar tablets by gavage， and the dimethyl fumarate group was given 50 mg/（kg·d） of dimethyl fumarate solution. The blank group and the model group were given 10 ml/（kg·d） of distilled water by gavage. Gavage was administered once daily for 14 days. Behavioral changes were evaluated using the open field test （total distance， central area distance， and average speed）， rotarod test （time on the rod）， and climbing pole test （climbing time）. Serum levels of interleukin-1β （IL-1β）， interleukin-18 （IL-18）， and myeloperoxidase （MPO） were measured by ELISA. Immunohistochemistry was used to detect tyrosine hydroxylase （TH） expression in the brain substantia nigra. Immunofluorescence was used to detect α-synuclein （α-syn） expression. Western Blot was used to detect Nrf2， NLRP3， Caspase-1， and α-syn protein levels in the brain substantia nigra. RT-PCR was used to detect mRNA expression levels of Nrf2， NLRP3， and Caspase-1 in the brain substantia nigra. ResultsCompared with the blank group， the model group showed decreased total distance， central area distance， and average speed， reduced time on the rotarod， prolonged climbing time， reduced TH expression， increased α-syn expression， decreased Nrf2 protein and mRNA expression， increased NLRP3 and Caspase-1 protein and mRNA expression， and elevated serum IL-1β， IL-18， and MPO levels （P＜0.05）. Compared with the model group， all drug interventions significantly improved the above indicators （P＜0.05）. There was no significant differences in all indicators between the high-dose BHG group and the Madopar group （P＞0.05）. Compared with the dimethyl fumarate group， the medium and high-dose BHG groups showed increased Nrf2 mRNA expression in the brain substantia nigra （P＜0.05）. Compared with the high-dose BHG group， the low-dose group showed decreased total distance， central area distance， and average speed， reduced serum IL-18 levels， decreased α-syn， Nrf2， NLRP3， and Caspase-1 protein levels， and lower Nrf2 mRNA expression （P＜0.05）. ConclusionThe mechanism by which BHG treat PD may involve activating the Nrf2/NLRP3 inflammasome axis in the brain substantia nigra， thereby reducing neuroinflammation and α-syn aggregation. The high-dose group showed the best effects.