Objective To explore the effect on the differentiatiation of bone marrow mesenchymal stem cells(BMSCs) induced by Ganglioside(GM1) into neural cells.Methods Rat BMSCs were isolated on the basis of its ability to adhere to the culture plate,passaged three times,and finally added GM1 to induce its differentiation.The morphologic changes of BMSCs were observed under phase-contrast microscopy,then neuron specific enolase(NSE) and glial fibrillary acidic protein(GFAP) expression were determined by immunocytochemistry way.Never growth factor(NGF) mRNA and brain-derived neurotrophic factor(BDNF) mRNA expression were detected with RT-PCR and then compared with those of FBS control group and blank control group.Results In GM1 induction group,BMSCs appeared round-shaped,with some dendrite and axon interlaced.(29.47%?3.26)% BMSCs showed positive staining to NSE,while(2.32?0.18)% were positive to GFAP.(6.97?0.56)% and(10.6?0.75)% BMSCs were positive to NSE in FBS control group and blank control group respectively,while(1.41?0.35)% and(1.21?0.35)% BMSCs were positive to GFAP in FBS group and blank control group respectively.NGF mRNA and BDNF mRNA levels in GM1 induction group were significant higher than those in the FBS group(all P

Objective To investigate the relationship between plasma resistin level and insulin resistance in the patients with cerebrovascular disease.Methods Fasting plasma resistin and insulin (INS) protein levels were determined by ELISA method in 50 patients with atherothrombotic cerebral infarction (ACI), 36 patients with lacunar infarction (LI), 36 patients with intracerebral hemorrhage (ICH) and 46 healthy control subjects. Blood glucose, blood lipid, blood pressure, height and weight were measured. Body mass index (BMI) and quantitative insulin sensitivity check index (QUICKI) were caculated as well.Results Compared with the control subjects, there were significantly higher fasting plasma insulin protein level and lower QUICKI in the ACI and ICH patients ( P

Objective To investigate the change and its clinical significance of plasma insulin and leptin level in the patients with cerebral infarction.Methods The plasma leptin, insulin, glucose levels and insulin sensitivity index(ISI) of 31 patients with atherothrombotic cerebral infarction (ACI), 30 patients with lacunar infarction (LI) and 21 cases of healthy controls were determined. Results Compared with those in the controls, there were elevation of plasma insulin level (6.17 ? 4.33 ?IU/ml, P

0.05).Conclusion The gene polymorphism of MMP-9/C1562T may be unrelated with IS.

Objective To establish a rapid determination of sodium valprate in plasma by capillary zone electrophoresis.Method Plasma was acidized by 1 mol/L HCl, VPA was extracted into organic phase (n-Hexane), then re-extracted into aqueous phase (0.05 mmol/L NaOH solution including 15% Ethanol). Capillary clone was 75 ?m(id)?37 cm. Electrolyte consisted of 15 mmol/L sodium salicylate, 0.5 mmol/L CTAB and 15% Ethanol (pH 5.7).Separating voltage was 20 kV, detection wave was 214 nm, temperature was 20℃,injection time was 5 s by press in negative.Result The linear ranger of concentration for standard drug was between 25~200 ?g/ml (r=0.999),the limit of detection was 0.35 ?g/ml, the average recovery of VPA was 87.4%,the average inter-day and intar-day CV were less than 4% and 6%. Conclusion This method is simple, rapid, reliable and correct, for determination of VPA in plasma.

To analyze maternal and neonatal complications among late preterm birth cases and to investigate risk factors of late preterm birth. Methods This was a retrospective analysis of 258 late preterm cases (late preterm group) born in Peking University First Hospital from January 1, 2009 to December 31, 2010. Maternal comorbidity and complications, delivery modes, and neonatal complications of these 258 late preterm infants were compared with 308 term cases (term group) during the same period. Statistical analysis was performed usingχ2 test, Fisher's exact probability test, t test and logistic regression. Results In Peking University First Hospital, late preterm births accounted for 3.9%(258/6 695) of live births and 60.1%(258/429) of preterm births. The incidence of the following maternal complications among the late preterm group was higher than that among term group(all P<0.05): severe pre-eclampsia [7.4%(19/258) vs 1.0%(3/308), χ2=15.35]; preterm rupture of membrane [42.6%(110/258) vs 15.3%(47/308), χ2=52.49];cervical insufficiency [1.9%(5/258) vs 0.0%(0/308), Fisher's exact test];placenta previa[3.5%(9/258) vs 0.6%(2/308), Fisher's exact test] and placental abruption [2.7%(7/258) vs 0.3%(1/308), Fisher's exact test]. Severe pre-eclampsia was the major risk factor leading to late preterm birth. The incidence of the following neonatal complications among the late preterm group was higher than that among term group (all P<0.05):respiratory distress syndrome (NRDS) [11.6%(30/258) vs 1.6%(5/308), χ2=24.22]; hyperbilirubinemia [64.3%(166/258) vs 39.6%(122/308),χ2=34.36];electrolyte disturbance [12.8%(33/258) vs 1.6(95/308),χ2=27.96];hypothermia [7.0%(18/258) vs 2.9%(9/308),χ2=5.08];infectious pneumonia[13.6%(35/258) vs 3.2%(10/308), χ2=20.43]; leukoencephalopathy [3.1%(8/258) vs 0.3%(1/308), χ2=5.25]; low body temperature [18.6%(48/258) vs 3.6%(11/308),χ2=33.98] and neonatal asphyxia [6.2%(16/258) vs 1.0%(3/308),χ2=11.86]. The incidence of the following neonatal complications among late preterm infants born at<35 weeks gestation was higher than that among late preterm infants born at≥35 weeks gestation (all P<0.05):NRDS [30.4%(14/46) vs 7.5%(16/212) ,χ2=19.26];hyperbilirubinemia [91.3%(42/46) vs 58.5%(124/212), χ2=17.74]; electrolyte disturbance [21.7%(10/46) vs 10.8%(23/212), χ2=4.02]; intracranial hemorrhage [8.7%(4/46) vs 1.9%(4/212),χ2=3.88];leukoencephalopathy [10.9%(5/46) vs 1.4%(3/212),χ2=8.32] and neonatal asphyxia [15.2%(7/46) vs 4.2%(9/212), χ2=6.05]. Conclusions Severe pre-eclampsia is the major risk factor leading to late preterm birth. The incidence of complications among late preterm infants is higher than that among term infants. If a pregnancy has to be terminated because of maternal disorders, the pregnancy period should be extended to 35 weeks if it permits.

Objective To investigate the association of serum angiotensin converting enzyme(ACE)activity and ACE gene polymorphism with vascular dementia(VD)and Alzheimer's disease(AD).Methods Using the polymerase chain reaction(PCR),ACE gene polymorphism was analyzed in 62 patients with VD,39 patients with AD and 50 healthy controls.The ACE activity in 56 patients with VD,33 patients with AD and 46 healthy controls was measured by means of capillary electrophoresis ultraviolet detection.Results The ACE activity showed no significant difference between VD,AD and healthy controls.We did not find any association of ACE gene polymorphism in patients with VD.The frequency of I allele was significantly higher in AD group than that in the controls(P

Objective To investigate the association of the gene polymorphism of plasminogen activator inhibitor 1 (PAI 1) with the cerebral infarction and recurrent cerebral infarction (RCI). Methods The plasma PAI 1 activity, by means of chromgenic substrate assay, and the sequence polymorphisms of 4G/5G in promotor region and (CA)n dinucleotide repeats in the 4th intron of PAI 1 gene, by amplified fragment length polymorphism assay, were measured in 50 patients with first ever cerebral infarction (FCI), 45 patients with RCI and 60 healthy controls.Results The plasma PAI 1 activities in both FCI patients (1.13?1 1 AU/ml) and RCI (1.13?0.150 AU/ml) were remarkably higher than that in the controls (0.7?0.25 AU/ml) (both P

Objective To investigate the role of mutation of insulin receptor(IR) gene on the development of ischemic cerebral vascular disease.Methods The base variations at exon 17 and 20 of IR gene, by means of PCR SSCP were determined in the 68 cases of atherothrombotic cerebral infarction (ACI), 81 cases of lacunar infarction (LI) and 62 healthy controls.Results There were two alleles of T and C at exon 17 of IR gene. The prevalence of mutant of T allele in ACI patients was more common than that in the controls. The blood pressure and the parameters of blood sugar,lipid metabolism in the patients with mutant were higher than those in the controls with wild type gene. The correlative analysis showed the polymorphism of IR gene was not related statistically to the blood pressure. No base variation at exon 20 was found in the study.Conclusion By promoting the development of atherosclerosis,the mutation at exon 17 of IR gene may participate in the occurrence of ischemic stroke.

Objective To study the changes of plasma renin activity(PRA) and angiotensin Ⅱ(Ang Ⅱ) concentration in patients with acute cerebral infarction(ACI) as well as their clinical significance.Methods Radioimmunoassay was used to detect PRA and Ang Ⅱ concentration in 55 patients with ACI in the acute,convalescent phrase,and carried out correlative analysis with focal size and the degree of neurological deficit.Results (1) PRA and Ang Ⅱ in 3d of the acute phase of ACI were significantly higher than those in the controls ( P 3.0 cm 2) and the focus of middle infarct group(1.5 to 3.0 cm 2),but PRA and Ang Ⅱ in both groups were significantly higher than those in lacunar infarction group(