1.Clinical characteristic of diagnosis and treatment of traumatic diaphragm rupture
Lihua ZHANG ; Zhongliang CHEN ; Yingcong ZHENG
Chinese Journal of Primary Medicine and Pharmacy 2006;0(03):-
Objective To investigate the characteristic of diagnosis and treatment of traumatic diaphragm rupture.Methods The characteristic of diagnosis and treatment of 32 cases with traumatic diaphragm rupture was analyzed retrospectively.14 cases were injured by sharp instrument and the other cases with blunt injury mainly caused by traffic accidents.Results Of 32 cases,19 had diaphragmatic ruptures preoperatively,13 diaphragmatic rupture was found during the operation.28 cases cured and 4 cases died.Conclusions The mechanism of traumatic diaphragm rupture and patients' symptoms and sign are severe and complicate with a high shock and mortality.Correct diagnosis and management of traumatic diaphragm rupture are very important.The treatment principle is thoracic injures first and laparotomy second.
2.Functional division and analysis of hospital administrators in French government
Manli CHEN ; Li XIANG ; Zhiyong LIU ; Yingcong ZHANG ; Lan YAO
Chinese Journal of Hospital Administration 2012;28(4):316-319
French hospital administrators have undergone several changes to meet the needs of economic and social development,resulting in the pattern of hospital administration by both the central government and regional organizations.This paper made descriptions and analysis of the French hospital administrators and came up with the following insights:Making public hospitals as the main health service provider benefits provision of public services; public and private hospitals can jointly meet public service needs under government regulation; the government should focus on controlling health care resources;medical service system needs an overall policy guidance; hospital management organizations should streamline in the reform.
3.Reasons for anastomotic leakage following the learning curve by laparoscopic anterior resection of rectal cancer
Donghui ZHANG ; Kui HE ; Zhehong ZHUANG ; Jianbao ZHANG ; Yingcong LIUFU ; Zhihao LIANG ; Chaojun ZHANG
Journal of Central South University(Medical Sciences) 2017;42(7):814-819
Objective:To investigate the reasons of anastomotic leakage following learning curve by laparoscopic anterior resection of rectal cancer.Methods:From December,2011 to March,2015,the clinical information of 179 patients in our hospital who underwent dixon of rectal cancer were collected.The patients were divided into a laparoscopic learning group,a laparotomy group and a laparoscopic group,The reasons of anastomotic leakage for each group were comparatively analyzed.Repeated cutting of anastomotic stoma was compared between the laparoscopic learning group and the laparoscopic group.The male,age,obesity,nutrition complications and the position of anastomotic stoma were compared among the 3 groups.Results:The rate of anastomotic leakage in the laparoscopic learning group was significantly higher than that in the laparotomy group and the laparoscopic group (P<0.05).Repeated cutting was a significant risk factor in the laparoscopic learning group (P<0.05),but not in the laparoscopic group.Except obesity,the four factors were significant risk factors in the laparoscopic learning group (P<0.05).All of the five factors were not the significant risk factors in the laparotomy group and the laparoscopic group (P>0.05).Conclusion:The operation technical shortcoming is the major factor in the learning of the laparoscopic anterior resection of rectal cancer.In order to reduce the rate of anastomotic leakage in the learning curve period,the selection of patients following the laparoscopic anterior resection of rectal cancer should avoid the following factors:male,older age,the low position of the tumor and the nutrition complications.
4. Therapeutic response of radiosynovectomy with p-32 colloid in 326 patients with hemophilic arthropathy
Zhen WANG ; Yin ZHANG ; Yinghui GE ; Huijuan LIU ; Yanhui LIU ; Jiajun ZHAO ; Yingcong DOU ; Pingchong LEI
Chinese Journal of Hematology 2017;38(1):39-43
Objective:
To observe the therapeutic response of radiosynovectomy with p-32 colloid on hemophilic arthropathy, and to assess the effects of radiosynovectomy with Denver Score on hemophilic arthritis staging.
Methods:
Radiosynovectomy with p-32 colloid was performed on 326 hemophilic arthritis patients (405 joints) , and recorded bleeding before and after treatment. The MRI performance of 102 joints was evaluated by using Denver scoring system, then was divided into 0-6 and 7-10 groups. Finally, the differences between 2 groups were analyzed.
Results:
Average pain score of all hemophilic arthritis patients at 6 months, 1, 2 and 3 years post treatment decreased from 3.2±2.4 (
5.Usefulness of self-made gasbag double-cannula stool drainage device for prevention of anastomotic leakage following anterior resection.
Donghui ZHANG ; Kui HE ; Huaiyu QIU ; Zhehong ZHUANG ; Yingcong LIUFU ; Jianbao ZHANG ; Xinchen ZENG
Chinese Journal of Gastrointestinal Surgery 2017;20(8):914-918
OBJECTIVETo evaluate the efficacy of self-made gasbag double-cannula stool drainage device for prevention of anastomotic leakage following anterior resection.
METHODSClinical data of 169 rectal cancer patients in the 8th Affiliated Hospital of Sun Yat-sen University between October 2010 and October 2016 were retrospectively analyzed. Among them, a self-made gasbag double-cannula stool drainage device was placed in 71 patients(stool drainage group), and the remaining 98 patients were taken as control. After an anastomosis, the drainage device was transanally placed by the assistant and the distal tube of drainage device was stretched more than 15 cm from anastomosis. The gasbag was inflated to fully expand the intestine. The main tube was fixed on perianal skin with 7-0 suture, kept more than 3-5 cm outside the anus, and connected to the drainage bag. The incidence of anastomotic leakage was compared between the two groups.
RESULTSThe baseline data were similar between the two groups (all P>0.05). The differences in operative time, intraoperative blood loss, and time to bowel function recovery were not statistically significant (all P>0.05), however, time to oral intake and postoperative stay were shorter in stool drainage group as compared to the control group (both P<0.05). There was no perioperative death in both groups. In stool drainage group, there were 6 cases whose drainage device was pulled out within 48 hours due to intolerance. The ruptured gasbag was replaced 5 times and the tube was clogged by fecal material 21 times. After flushing, the tube did not recanalized and was pulled out in 3 cases. The incidence of anastomotic leakage in stool drainage group was significantly lower than that in the control group (2.8% vs. 11.2%, P=0.043). As for the low anastomosis (the distance to anal verge less than 5 cm), the incidence of anastomotic leakage in stool drainage group was also significantly lower than that in the control group (2.3% vs. 15.4%, P=0.028), while as for the high anastomosis, the difference was not statistically significant (3.6% vs. 3.0%, P=0.906). Logistic regression analysis revealed that the presence of a stool drainage device was an independent protective factor for anastomotic leakage (OR=0.316, 95%CI:0.114 ~ 0.769, P=0.003).
CONCLUSIONSThe self-made gasbag double-cannula stool drainage device effectively prevents anastomotic leakage after anterior resection of rectal cancer. However it is not suitable for those patients with high anastomosis.
6.Mycobacterium vaccae induces a strong Th1 response that subsequently declines in C57BL/6 mice.
Lijiao ZHANG ; Yanlong JIANG ; Ziyin CUI ; Wentao YANG ; Limin YUE ; Yingcong MA ; Shaohua SHI ; Chunfang WANG ; Chunfeng WANG ; Aidong QIAN
Journal of Veterinary Science 2016;17(4):505-513
Mycobacterium (M.) vaccae is a fast-growing species of saprophytic bacteria that is widely distributed. To understand the host immune responses induced by M. vaccae isolated from bovine submaxillary lymph nodes, C57BL/6 mice were infected with reference strain M. vaccae Bacillus Calmette-Guérin (BCG) and isolated M. vaccae using intraperitoneal injections. Comparison of the bacterial replication and organ pathology between M. vaccae and M. vaccae BCG revealed that M. vaccae was more malignant than M. vaccae in mice. We also demonstrated that serum from the M. vaccae-infected mice contained a higher expression level of gamma-interferon (IFN-γ), tumor necrosis factor alpha, monocyte chemoattractant protein-1, interleukin (IL)-4, IL-12, IL-10 and transforming growth factor beta than did the other groups, especially after week 4. Furthermore, when the numbers of CD3⁺CD4⁺IFN-γ⁺ and CD3⁺CD4⁺IL4⁺ cells in the infected mice were observed by flow cytometry, we found that a powerful T helper 1 (Th1) response was induced by M. vaccae infection, which was associated with the emergence of CD3⁺CD4⁺IFN-γ⁺ cells. However, the Th1 response declined over time, which was associated with appearance of the CD4⁺CD25⁺FoxP3⁺ and CD4⁺CD25⁺CD152⁺Treg cell reaction. In addition, a strong Th2 response was found. Finally, we found that M. vaccae infection increased the production of type I IFNs, which was associated with a reduced Th1 response.
Animals
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Bacillus
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Bacteria
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Chemokine CCL2
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Flow Cytometry
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Injections, Intraperitoneal
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Interferon-gamma
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Interleukin-10
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Interleukin-12
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Interleukins
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Lymph Nodes
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Mice*
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Mycobacterium bovis
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Mycobacterium*
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Pathology
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Transforming Growth Factor beta
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Tumor Necrosis Factor-alpha
7. Influence of phosphoglycerate kinase 1 on metastasis and invasion of hepatoma cells and prognosis of liver cancer
Lan ZHANG ; Yingcong WANG ; Xiaoying XIE ; Jun CHEN ; Dongmei GAO ; Zhenggang REN
Chinese Journal of Hepatology 2017;25(6):446-451
Objective:
To investigate the association between expression of phosphoglycerate kinase 1 (PGK1) in liver cancer tissue and prognosis, as well as its influence on metastasis and invasion of hepatocellular carcinoma (HCC) cells.
Methods:
Overexpression and downregulated expression of PGK1 in HCC cells were mediated by lentivirus to establish hepatoma cell lines with different expression levels of PGK1. The Transwell chamber invasion assay, wound healing assay, and colony-forming assay were used to investigate the influence of PGK1 on metastasis, invasion, and proliferation of HCC cells. Immunohistochemistry was used to measure the expression of PGK1 in liver cancer tissue samples from 116 patients with hepatocellular carcinoma who underwent radical surgery, and the Kaplan-Meier method and the log-rank test were used to determine the association between PGK1 expression and prognosis of patients with liver cancer.
Results:
HCCLM3 and MHCC97H HCC cells with high metastatic potential had significantly higher expression of PGK1 than Hep3B and Huh7 HCC cells with low metastatic potential. Downregulation of PGK1 expression significantly inhibited the migration (31.2% ± 2.4% vs 12.0% ± 1.3%,
8.Challenges and countermeasures on the translation of scientific research achievements of public hospitals in China
Jiao YUAN ; Yangzheng LIU ; Qingsong WU ; Can XIANG ; Yingcong ZHANG ; Lin YE
Chinese Journal of Hospital Administration 2020;36(11):951-954
Public hospitals play an important role in independent innovation of basic medicine and clinical medicine. As key players in translation of medical research achievements, they play a pivotal role in promoting the development of medical sciences. At present, these hospitals however, are faced with challenges in the translation, namely system defects, unclear property rights ownership, misleading research project goals, defective assessment mechanism, insufficient funding, and lack of translation channels. In such considerations, the authors suggested based on their experiences to improve the translation system of scientific research achievements, set up special departments for translation, clarify the rights to use and dispose of scientific research achievements and that to yields. They also proposed to actively guide the establishment of clinical application projects, improve the evaluation mechanism, and broaden channels of funding. These measures are expected to accelerate the translation of scientific research achievements.
9.Research progress of phosphoglycerate mutase 5-mediated mitophagy and necroptosis
Jing ZHANG ; Miao CHEN ; Xinxin LIU ; Yingcong REN ; Guoyue LIU ; Song QIN
Chinese Critical Care Medicine 2022;34(8):890-896
Mitophagy is the selective degradation of damaged mitochondria, and it is of great significance to maintain the normal quantity and quality of mitochondria to ensure cell homeostasis and survival. Necroptosis is a type of programmed cell necrosis that can be induced by excessive mitophagy. Reactive oxygen species (ROS) are produced mainly by mitochondria and can damage mitochondria. Hyperoxic acute lung injury (HALI) is a serious complication of clinical oxygen therapy, and its pathogenesis is not clear. Existing studies have shown that mitophagy and necroptosis are involved in the occurrence of HALI. There are many mechanisms regulating mitophagy and necroptosis, including tumor necrosis factor-α (TNF-α), E3 ubiquitin protein ligase (PINK1/Parkin) protein pathway encoded by PTEN-induced kinase 1/PARK2 gene, phosphoglycerate mutase 5 (PGAM5), etc. PGAM5 has been proved to be a key factor linking mitophagy and necroptosis. Previous studies of our team found that the mechanism of microRNA-21-5p (miR-21-5p) alleviating HALI was related to its pGAM5-mediated inhibition of mitophagy, but the mechanism of PGAM5-mediated mitophagy and necroptosis remains unclear. Therefore, this paper reviews the targets of PGAM5-mediated mitophagy and necroptosis, in order to find clues of lung protection of pGAM5-mediated mitophagy and necroptosis in HALI, and provide theoretical basis for subsequent basic research.
10.The role and thinking of expert preliminary counselling in improving the funding rate of National Natural Science Fund
Zhihui WANG ; Yingcong ZHANG ; Fei LUO ; Shi CHEN ; Qingsong WU ; Jiancai WU ; Lin YE ; Can XIANG
Chinese Journal of Medical Science Research Management 2021;34(3):187-190
Objective:In this study, we compared the funding rates of applicants who participated in the expert preliminary counseling with those who did not, to explore the role of the expert preliminary counseling in improving the funding rate of the National Natural Science Fund, so as to provide further reference for the management of scientific research.Methods:Statistical methods was used to analyze relevant data from 2012 to 2019 to compare the approval and funding rate of the applicants that participated in and did not participate in the expert preliminary counseling using.Results:From 2012 to 2019, the proportion of funded applicants in the expert preliminary counselling group was 30.45%, and the overall funding rate was 18.51%. Under the same research basis, the funding rate of the group was 29.91%, which was significantly higher than the group did not participate in the expert preliminary counselling (funding rare: 13.59%, P<0.001). Conclusions:We should pay more attention to and participate actively in the expert preliminary counselling, which can increase the quality of the projects and improve their funding rate significantly.