OBJECTIVETo study the change rule of the hemodynamics of expanded skins and research the transplant time of random-pattern flaps that are made of the expanded skin.

METHODS72 ears of 64 rabbits were used as a subject of experiment. Four items of the contrasted skin (n = 8) and expanded skin (n = 8 x 8), Which include the microvessel diameter, the microvessel density, the blood velocity and the blood volume, were observed and measured by the microcirculatory imaging computer analysis system, the enzyme histochemical dyeing and the more functions colored pathological imaging computer analysis system. The length -width proportion and the largest area of the flaps that had made of the expansive skin and survived were measured.

RESULTS(1) The four items of expanded skin, that the microvessel diameter, the microvessel density, the blood velocity and the blood volume are 13.43 +/- 0.98 micrometer, 0.0472 +/- 0.0022, 1012.70 +/- 65.51 micrometer/s, 14.71 +/- 0.74 micrometer(3)/s respectively, are much bigger or quicker than those of contrasted skin [(7.22 +/- 0.71) micrometer, 0.0108 +/- 0.0002, (327.01 +/- 65.51) micrometer/s, (1.46 +/- 0.41) micrometer(3)/s] and there is the significant difference between both (6.49 < t < 49.49, P < 0.01). (2) The blood velocity increases in the expanding phases and always keeps on higher level. The other items all change periodically. Span of the period is four weeks. The microvessel diameter and the blood volume have a peak value at third weekend of every period and in the later period enhance more on the basis of the former period. The microvessel density changes in definite range in every period. (3) The surviving area of the random skin flaps is the largest which were made of the expanded skin in third weekend of every period, it changes more than those of other weekends in same period and later period is superior to the former period. There is a significant difference compared with contrasted random skin flaps (q = 3.7215, P < 0.05; q = 5.5026, P < 0.01).

CONCLUSIONThe hemodynamics of the expanded skin changes periodically in expansive course. The time of peak value of the blood volume is the best opportunity that random-pattern skin flaps are made of the expanded skin in every period.

Animals ; Blood Flow Velocity ; Capillaries ; physiology ; Hemodynamics ; Rabbits ; Skin ; blood supply ; Surgical Flaps

Objective To investigate whether Tian Jiu therapy can regulate Th1/Th2 ratio effectively for bronchial asthma patients.Methods A total of 30 bronchial asthma patients who were treated with Tian Jiu therapy in the hospital were enrolled in the study as patients group and 20 healthy individuals were enrolled as control group.The mRNA of T-bet,GATA3,Foxp3 and STAT-6 were detected by using realtime-PCR,while IFN-γ and IL-4 were detected by using ELISA for control group and patents group before and after treatment. Flow cytometry was used to detect Th1/Th2 ratio.Results After Tian Jiu therapy,Th2 proportion decreased and Th1/Th2 ratio increased,secretion of IFN-γ decreased,mRNA of T-bet and GATA3 significantly in-creased,while STAT-6 decreased significantly(P<0.05).Conclusion Tian Jiu therapy has therapeutic effect on bronchial asthma patients through decreasing Th2 proportion and regulating Th1/Th2 ratio.

OBJECTIVE: To explore the prenatal screening and diagnosis for a pair of monochorionic-diamniotic (MCDA) twins discordant for 45,X/46,XX mosaicism. METHODS: Amniotic fluid samples were taken from both twins for whom non-invasive prenatal testing has signaled a high risk for sex chromosomal abnormality. Uncultured amniotic fluid was analyzed by fluorescence in situ hybridization (FISH) and single nucleotide polymorphism array (SNP-array). Conventional G-banded karyotyping analysis was performed on the cultured amniotic fluid. RESULTS: Metaphase chromosome analysis showed that one of the twins had a mos 45,X[11]/46,XX[26] karyotype, while the other had a normal karyotype. FISH and SNP-array applied on uncultured amniotic fluid revealed about 30% mosaicism in one of the twins. The twins were confirmed to be monozygotic by SNP-array analysis. CONCLUSION To avoid confusion arising from discordant karyotypes in MCDA twins with abnormal non-invasive prenatal testing (NIPT) results, dual amniocentesis should be carried out to obtain amniotic fluid samples for chromosomal as well as molecular analysis. To determine the ratio of 45,X and 46,XX cells in Turner syndrome can provide valuable information for prenatal genetic counseling.
Amniocentesis ; Chromosomes, Human, X ; Female ; Humans ; In Situ Hybridization, Fluorescence ; Karyotyping ; Mosaicism ; Pregnancy ; Prenatal Diagnosis