Objective To evaluate the performance of FL-1 macroporous absorption resin for absorption of total flavonoids from Psidium guajava leaf.Methods The concentration of total flavones in Psidium guajava leaf was determined by ultraviolet spectrophotometry and the absorption behavior of FL-1 macroporous adsorption resins to total flavones in Psidium guajava leaf was examined for the adsorption capacity and the volume of solution loaded.Results Optimal absorption of total flavonoids was achieved with the sample:total flavonoids concentration in the solution of 13.22 mg/mL,the ratio of total flavonoids concentration and macroporous adsorption resin was 1∶4,washing with 70% ethanol at the flow velocity of 2 BV/h.Conclusion FL-1 Macroporous absorption resin can be well applicable for enrichment of total flavonoids in Psidium guaijava leaf.

Objective To investigate the protective role of two-step decapping technique for superior parathyroid glands in total thyroidectomy surgery.Methods The clinical data of 136 consecutive cases underwenttwo-step decapping technique in total thyroidectomy plus central compartment dissection from April 2013 to January 2016 were retrospectively analyzed.And the incidences of postoperative hypocalcemia were was compared with conventional consecutive decapping surgical methods containing 113 cases from January 2010 to March 2013.All operations were performed by the same treatment group.Results In the two-step decapping group,28 cases(28/136,20.6％) displayed postoperative temporary hypoparathyroidism and a higher incidence were was observed in 37 cases (37/113,32.7％) in the conventional method group,the difference was statistically significant(x2 =4.737,P =0.03).Whereas,2 cases(2/136,1.5％) suffered permanent hypoparathyroidism in the two-step decapping group and 7 cases (7/113,6.2％) of permanent hypoparathyroidism in the conventional method group,the difference was statistically significant (x2 =3.954,P =0.047).Conclusion Two-step decapping technique can more effectively protect the parathyroid gland function.It is worthy of popularization.

Objective To elucidate the regulate role of the guava leaf on the levels of IFN-? and IL-2 in the salivary juice on children patients with noroviruses diarrhea. Method 50 patients with noroviruses diarrhea was grouped randomly to group A (30 patients), which was treated with guava leaf and group B (20 patients), which was treated with Smecta, and 20 health children was randomly choiced as normal control group. The levels of IFN-? and IL-2 in the salivary juice of the three groups were determined and compared before and after the treatments. Results The levels of IFN-? and IL-2 in the group A and B before the treatments were higher than the normal control group (P

Objective To investigate the protection of superior parathyroid glands by improving dissection procedure of thyroid superior pole in total thyroidectomy surgery.Methods 136 consecutive cases undergoing two-step dissociating procedure of thyroid superior pole(the improved group) in total thyroidectomy plus central compartment dissections from Sep.2012 to Oct.2015 were retrospectively analyzed.Intraoperative observation of superior parathyroid and incidences of postoperative hypoparathyroidism were compared with conventional surgical methods (the conventional group) containing 113 cases from Jun.2009 to Sep.2012.All operations were performed by the same treatment group.Results Intraoperatively,249 (249/272,91.5％) superior parathyroid glands were identified in the improved group and 215 (215/226,95.1％) superior parathyroid glands were identified in the conventional group.The difference had no statistical significance (P＝0.114).Whereas,among identified superior parathyroid glands,242 (242/249,97.1％)of them were conserved in situ in the improved group and 198 (198/215,92.0％) were conserved in situ in the conventional group.The difference had no statistical significance (P＝0.013).Among superior parathyroid glands conserved in situ,225 (225/242,93.0％) had good blood supply in the improved group and 170(170/198,85.9％) in the conventional group.The difference had statistically significance (P＝0.001).28 (28/136,20.5％) patients displayed temporary hypoparathyroidism and 2 (2/136,1.5％) cases suffered permanent hypoparathyroidism in the improved group after surgery.Whereas,in the conventional method group,a higher incidence was observed in 37 (37/113,32.7％) cases of temporary hypoparathyroidism and 7 (7/113,6.2％) cases of permanent hypoparathyroidism respectively.The difference had statisticall significance (P＝0.003).Conclusions Two-step dissociating procedure technique offers protective effect on superior parathyroid glands function.It is worthy of popularization.

Objective To investigate the effect of Alzheimer' s beta-amyloid (Aβ) on the production and the translocation in cytoplasm of retinoid receptor-α (RXRα). MethodsN2awt cells were treated with Aβ peptide or amyloid protein precursor(APP695) transfection. The nucleus were separated from the cytoplasm by kit. The quantity of RXRα in the nucleus and cytoplasm was detected by Westernblot. The translocation of RXRα in the nucleus and cytoplasm of above N2awt cells or of the cortex cells in the brains of Alzheimer' s disease (AD) patients and their normal control groups was observed by immune fluorescence. ResultsIn N2awt cells, the increasing APP or Aβ had no significant effect on the production of RXRα but resulted in RXRα exporting into cytoplasm, the ratio of RXRα in cytoplasm increased from 3.2％ (in control group) to 17.6％ (in APP+ group) and from 3.8％ (in control group) to 14.3％ (in Aβ + group) respectively; compared with normal cortex cells, the translocation of RXRα in the cytoplasm of the cortex cells in the brains of AD increased significantly. ConclusionAβ may affect RXRα exporting into cytoplasm.

BACKGROUND: Many studies showed that neural stem cells (NSC) transplantation can promote functional improvements in rats subjected to spinal cord injury. However, the underlying molecular mechanisms remain poorly understood.OBJECTIVE: To observe the effects of NSC transplantation on expressions of Bax, Bcl-2 and Caspase-3 in the motor cortex in rats subjected to spinal cord transection.DESIGN, TIME AND SETTING: The randomized controlled animal experiment was performed at Institute of Neuroscience,Kunming Medical College from July 2007 to December 2008.MATERIALS: Five green fluorescent protein transgenic mice with 14-15 embryonic days were prepared for NSC. Additionally 88 adult female Sprague-Dawley (SD) rats were randomly divided into sham operation group (n=8), model group (n=40) and NSC transplantation group (n=40).METHODS: Model of spinal cord transection was established by cut transversely Sprague-Dawley (SD) rats T_9 segment. Rats in the sham operation group were subjected to laminectomy at T_8, without spinal cord injury. After the spinal cord was exposed at the lesion site, a small piece of 2 mm~3 gel foam soaked with 3×10~5 NSC were implanted into the gap to fill the lesion site of the T_9 level in rats of the NSC transplantation group. The measurements of relative indexes were performed at the days 3, 7,14, 21 and 28 after transplantation.MAIN OUTCOME MEASURES: Changes of Bax, Bcl-2 and Caspase-3 expressions were detected by RT-PCR.RESULTS: Compared to the sham operation group, the Bax expression in the model group had no significant difference (P >0.05), the expression of Bcl-2 was decreased obviously at days 14 and 28 after operation (P < 0.05), while a significant increase on the expression of Caspase-3 at day 3 (P < 0.05). Compared to the model group, the expression of Bax was dramatically decreased in the NSC transplantation group at day 3 (P < 0.05), with a notably increased Bcl-2 expression at days 14 and 21 after transplantation (P < 0.05), but the expression of Caspase-3 presented a significant decrease at days 3 and 7 after transplantation (P < 0.05).CONCLUSION: NSC transplantation probably regulates the expression of apoptosis genes (Bax, Bcl-2 and Caspase-3 mRNA) to promote neurological function recovery in rats subjected to cord transection.

Objective To observe the effect of pigplacenta ins tead of Ziheche (P lacenta Hominis) for the treatment of senile dementia. Methods Seventy Kunming mice were divided into blank group, model group, pos itive control group, Zih eche low and high dosage groups, and pigplacenta low and high dosage grou ps, wi th 10 in each. The senile dementia models were established with the D-Galactose subcutaneous injection. The blank group was not administered any medicines. The model group was prescribed normal saline instead of the tested medicine; the pos itive control group was given Naofukang by gavage; the Zih eche low and high dosage groups were given 2g/kg and 4g/kg Ziheche respectivel y by gavage; while the pigplacenta low and high dosage groups were treated wi th p igplacenta 4g/kg and 8g/kg respectively by gavage. After treatment for 6 week s, the behavior experimental dark-avoiding test and step-down test were applied to test the effect of the medicines on the learning memory of mice, and acetylchol inesterase and monoamine levels in brain tissues. Results There was no s ignificant difference between the effect of pigplacenta and Ziheche in resi stin g senile dementia. In the latency of dark-avoiding test, the effect of high d osa ge of pigplacenta was significantly better than that of Ziheche (P

Advancements in technology have enhanced the performance of pacemakers but made the interaction between the pacemaker and the heart becoming increasingly complex, and its is difficult to interpret the paced ECGs (PGCGs). This paper introduces a brief method to interpret PECGs, which illustrate the timing intervals graphically and avoid using symbols and strings.

Objective:To explore the relationship between dyslipidemia and blood pressure variability (BPV)in pa-tients with essential hypertension.Methods:A total of 251 patients with essential hypertension (EH),who were treated in our department of outpatient from Jan 2011 to Dec 2012,were selected.There were 114 patients with EH complicated dyslipidemia were regarded as EH+dyslipidemia group and 137 pure EH patients reganed as pure EH group.All patients received ambulatory blood pressure monitoring (ABPM);BPV indexes were compared between two groups.Results:Compared with pure EH group,there were significant rise in standard variations (SD)of 24h systolic blood pressure (SBP)and diastolic blood pressure (DBP)[(10.59±2.04)vs.(14.96±4.12),(10.16± 2.11)vs.(12.34±3.19)],24h SBP coefficient of variation (SBPCV)[(0.13±3.07)vs.(0.91±2.21)];daytime (d)SBPSD and DBPSD [(11.27±1.97)vs.(13.98±4.03),(8.94±2.01)vs.(11.34±3.12)],dSBPCV [(0.79±0.21)比 (1.01±0.31)];nighttime (n)SBPSD and DBPSD [(7.96±3.14)vs.(13.22±5.37),(8.13±1.97) vs.(9.95±3.47)],and nSBPCV and nDBPCV [(0.78±0.18)vs.(0.09 ±0.43),(1.07±0.34)vs.(0.13 ± 0.37)]in EH+dyslipidemia group,P <0.01 all.Conclusion:There are relationship between dyslipidemia and blood pressure variability in patients with essential hypertension.

Objective To guide the implementation of home enteral nutrition (EN) for outpatients with Crohn's disease, and to observe its effect.Methods In this observational study, 26 patients at active period of Crohn's disease were collected between April 2014 and June 2015 in the clinic of Clinical Nutrition Center of Nanjing General Hospital of Nanjing Military Command.Home EN combined with medication was administered.The nutritional status, Crohn's Disease Activity Index (CDAI), and quality of life before and after EN were compared.Results After home EN therapy, body mass index (BMI) of the patients increased from (17.7 ± 2.1) kg/m2 to (19.8 ± 2.0) kg/m2 (P ＜ 0.001), proportion of lymphocytes from (0.216 ± 0.066) to (0.305 ± 0.106) (P =0.007), albumin level from (40.3 ± 5.9) g/L to (43.7 ± 5.4) g/L (P =0.038), prealbumin from (233.1 ± 71.5) mg/L to (306.0 ± 72.1) mg/L (P =0.009), and transferrin from (2.7 ± 0.6) g/L to (3.1 ±0.7) g/L (P =0.038).In the indicators reflecting the activity of Crohn's disease, CDAI declined from 197.0 ±55.8 to 113.2 ±33.4 (P ＜0.001) after EN, C-reactive protein from 28.50 (18.00-32.80) mg/L to 2.00 (0.58-6.33) mg/L (P ＜0.001), erythrocyte sedimentation rate from (29.6 ± 9.9) mm/h to (9.4 ± 7.6) mm/h (P ＜ 0.001).The patient's disease activity scores and inflammatory reaction indexes all reached the normal levels after EN, show that the patients were in remission stage.In inflammatory bowel disease quality of life score, the score of intestinal symptoms rose from 41.2 ± 13.3 to 57.0 ±9.6 (P ＜ 0.001), the score of systemic symptom from 20.7 ± 5.5 to 28.9 ± 4.2 (P ＜ 0.001), emotional ability from 43.6±16.1 to 61.0 ± 15.9 (P=0.002), and social ability from 20.6 ±4.9 to 28.4 ±5.6 (P＜0.001), all showing statistical significance.Conclusions Standardized home enteral nutrition in patients at active period of Crohn's disease, when implemented under professional guidance of nutritional support team and well followed up, may improve nutritional status, induce remission, and improved quality of life.