Objective To observe the effect of external use of Curcuma oil on the hyperplasia of mammary glands in rats and explore the mechanism. Methods Sixty female Wistar rats were randomly divided into following groups:normal con-trol group, model control group, Sanjierubi plaster group, low-, medium- and high-dose curcuma oil emulsion groups ( n=10 each).The models of hyperplasia of mammary glands were established by intramuscular injection of estradiol benzoate and proges-terone into the medial part of the rat hind limb.Different doses of medicines were given for 4 consecutive weeks.Enzyme-linked im-munosorbent assay, HE staining and immunohistochemistry were used to investigate the action mechanism of curcuma oil emulsion against mammary gland hyperplasia. Results Curcuma oil emulsion had preventive and therapeutic effects on the hyperplasia of mammary glands.The diameter of breasts was significantly reduced, the body weight restored, serum estradiol, follicle-stimulating hormone and prolactin levels profoundly decreased, progesterone, testosterone and luteinizing hormone levels markedly increased and the number and diameter of lobular acini obviously reduced in high-dose curcuma oil emulsion group when compared with those in model control group (P<0.05 or P<0.01). Conclusion Curcuma oil emulsion can remarkably improve the disturb-ance of serum hormones and inhibit the occurrence of hyperplasia of mammary glands.

Objective To prepare the hydroxysafflor yellow A (HSY-A)enteric coating pellets,and investigate in vitro release and in vivo pharmacokinetic characteristics.Methods HSY-A enteric coating pellets were prepared by extrude-rounding and fluid bed technique.The micromeritic characteristics,the factors affecting the release properties of enteric coating pellets the release mechanism were explored,and the in vivo pharmacokinetic behaviors were also evaluated.Results The in vitro release behavior of HSY-A from enteric coating pellets could be described by Ritger-peppas equation,and fit diffusion mechanism,in vivo pharmacokinetic test confirmed the argument that pellets have good acid residence and enteric properties. Conclusion HSY-A enteric coating pellets have been successfully prepared and the expected release properties is achieved in the study.

Objective To compare the effects of antibiotic-loaded bone cement coverage and negative pressure wound therapy (NPWT) on diabetic foot ulcer wound healing. Methods A total of 113 patients with diabetic foot ulcers were selected as study subjects and randomly divided into control group (57 patients receiving NPWT) and experimental group (56 patients receiving antibiotic-loaded bone cement coverage) using the double-color ball randomization method. Both groups underwent routine debridement. The treatment outcomes, positive detection rate of wound bacterial culture, lower limb nerve conduction function (tibial nerve motor conduction velocity, common peroneal nerve sensory conduction velocity), Doppler ultrasound indicators (vessel diameter, blood flow velocity, resistance index, pulsatility index), inflammatory indicators (tumor necrosis factor-α, interleukin-6, high-sensitivity C-reactive protein), and granulation tissue growth were observed and compared between the two groups. Results The effective treatment rate was 85.71% in the experimental group, which was higher than 70.18% in the control group (P < 0.05). Before treatment, there were no statistically significant differences between the two groups in terms of positive detection rate of wound bacterial culture, tibial nerve motor conduction velocity, common peroneal nerve sensory conduction velocity, blood flow velocity, vessel diameter, resistance index, pulsatility index, granulation tissue growth, and serum levels of tumor necrosis factor-α, interleukin-6, and high-sensitivity C-reactive protein (P>0.05). After 3, 7, and 15 days of treatment, the positive detection rate of wound bacterial culture in the experimental group was lower than that in the control group (P < 0.05). After treatment, the tibial nerve motor conduction velocity, common peroneal nerve sensory conduction velocity, vessel diameter, blood flow velocity, resistance index, and pulsatility index in both groups were higher or greater than those before treatment, and those in the experimental group were higher or greater than those in the control group (P < 0.05). After treatment, the granulation tissue area, coverage rate, and granulation tissue growth rate in the experimental group were higher than those in the control group (P < 0.05). After treatment, the serum levels of tumor necrosis factor-α, interleukin-6, and high-sensitivity C-reactive protein in both groups were lower than those before treatment, and those in the experimental group were lower than those in the control group (P < 0.05). Conclusion Compared with NPWT, antibiotic-loaded bone cement coverage has a higher effective treatment rate for diabetic foot ulcers, and provides a more effective local antibacterial environment, thereby promoting wound healing and tissue repair, and simultaneously improving nerve function and vascular status.