1.Expression regulation of nicotinic acetylcholine receptor subtype and its physiological significance
Yingfan ZHANG ; Hua JIANG ; Zihao LIN
Academic Journal of Second Military Medical University 1982;0(01):-
Nicotinic acetylcholine receptors concentrate on the postsynaptic membrane in the neuromuscular junction and participate in the neuromuscular transmission,which is of great importance to normal muscle function. The nicotinic acetylcholine receptor is a kind of pentamer.There are 2 types of nicotinic acetylcholine receptor due to the different subunit:fetal type ?-AChR (? 2???) and adult type ?-AChR(? 2???). This article reviews the difference of 2 types of nAChR in expression period,distribution,regulation mechanism and physiological significance.
2.Preparation and Therapeutic Effect of the Compound Nucleic Acid Granules
Wei WANG ; Gaofeng LIU ; Xiuping BAI ; Yanping LI ; Yingfan TANG
China Pharmacy 2001;12(5):276-277
OBJECTIVE: The compound nucleic acid granules were prepared, observed for their therapeutic effects, and determined for their pharmacological actions METHODS: Quality control was used in their preparation The therapeutic effects were observed on 22 patients with coronary heart disease of stable angina pectoris with hyperlipemia who were chosen according to clinical diagnosis standard RESULTS: ( 1) Improvment of clinical symptoms for angina pectoris: satisfactory 22 7% , improved 68 2% , failure 9 0% ; ( 2) Blood lipid analysis: cholesterols and triglycerides were improved significantly after administration of the preparation( P0.05;P<0.01).CONCLUSION:The compound nucleic acid granules have marked effects on coronary heart disease, angina pectoris and hyperlipemia.
3.Morphological changes at the motor end-plate following free muscle transfer
Him JIANG ; Antang LIU ; Yingfan ZHANG ; Baojin WU
Chinese Journal of Microsurgery 2000;0(03):-
Objective To investigate the changes at the motor end-plate (i. e. neuromuscular junction) following the free neurovascular muscle transfer. Methods Light and electron microscopy were used to study serially during 30 weeks the changes at the neuromuscular junction after free muscle transfer of the gracilis muscle in the adult Wistar rat. Results Under light microscopy, after staining with acetylthocholine the neuromuscular junction showed changes of degeneration with withdrawal of the innervating axon terminal followed by regeneration and reconstitution of the neuromuscular junction. With the electron microscope, mitochondrial sweelling and clumping of the synaptic vesicles were followed by withdrawal of the axon terminal from the muscle membrane on denervation. The infolding of the muscle membrane at the neuromuscular junction became less prominent. With reinnervation the ulatrastructure of the junction was only partially reestablishied with poorly reconstituted primary and secondary folds of the muscle membrane. The newly formed neuromuscular junction still lacked the structural detail seen in the control neuromuscular junction, even after 30 weeks. Conclusion The persistent weakness following free neurovascular muscle transfer may be attributed to the failure of full recovery of the intricate ultrastructure of the neuromuscular junction.
4.Establishing an experimental animal model of phallic reconstruction with sensory restoration
Baojin WU ; Hua JIANG ; Wenpeng LI ; Yingfan ZHANG
Academic Journal of Second Military Medical University 1982;0(01):-
Objective:To establish an experimental phalloplasty model with sensory restoration in rabbits and to explore a new surgical technique to improve the postoperative sensory function in phallic reconstruction. Methods: Adult male New Zealand white rabbit was used. Penile reconstruction was performed with superficial epigastric faciovascular pedicle flap. Free saphenous nerve graft was dissected, embedded in the flap, and end-to-end anastomosed to dorsal nerve of penis. Postoperatively, H-E staining and CGRP immunohistochemical staining were applied to observe the morphology of the regenerated nerves in the reconstructed penis at different stages. Results:Only a few CGRP-positive fibers scattered in deep corium in the reconstructed penis 1 month after surgery, while the quantity of CGRP nerve fibers increased markedly and the CGRP-positive fibers appeared in both superficial corium and papillae corii after 3 months. Six months postoperatively, the positive nerve fibers were also observed around the cutaneous accessories and in the epidermis-corium boundary layer. Conclusion: This rabbit model demonstrates the possibility of a new method for phalloplasty with sensory recovery, which may redound to clinical application.
5.The searches in teaching of thinking ability in animal surgery curriculum
Yingfan ZHANG ; Antang LIU ; Hua JIANG ; Laigen WANG
Chinese Journal of Medical Education Research 2005;0(06):-
The students are consciously taught how to think the surgery and how to analyze and solve problems in the animal surgery curriculum.
6.Clinical effects of needle free (INJEX30) versus traditional injection of botulinum toxin A for bromhidrosis
Fan FANG ; Yingfan ZHANG ; Qiang LI ; Yeqiong LI ; Zhiyong YANG
Chinese Journal of Medical Aesthetics and Cosmetology 2017;23(2):76-78
Objective To investigate the clinical efficacy of bromhidrosis with botulinum toxin A by needle free injection.Methods A total of 40 patients with bromhidrosis were collected and divided into needle free group and needle group.A comparative study was carried out in those subjects.Patients in needle free group received needle free injection of botulinum toxin A by INJEX30;Needle group received traditional injection by 26 G needles.Results In needle free group,16 cases were cured,3 cases were effective,1 case was ineffective,no significant pain,hematomas or necrosis were found.In needle group,15 cases were cured,4 cases were effective,and 1 case was ineffective;localized hematoma occurred in 2 cases.And cases in group needle experienced pain more intensely than cases in needle free group.Conclusions The INJEX30 improves the security,comfort and therapy compliance of botulinum toxin A injection in the bromhidrosis patients,with similar efficacy with traditional injection.
7.Application of SimMan human body simulator in nursing teaching experiments on emergency and critical care
Yingfan ZHANG ; Xiaoming XU ; Huan WANG ; Chengjian ZHANG
Modern Clinical Nursing 2016;15(5):55-59
Objective To explore the effect of application of SimMan human body simulator in nursing teaching experiments on emergency and critical care. Methods Forty undergraduate nursing students in 2012 were assigned as the experiment group, where nursing teaching experiments on emergency and critical care were conducted based on SimMan human body simulator. Forty undergraduate nursing students in 2011 were assigned as the control group, where the traditional teaching method was employed. The emergency ability of the nursing students in the two groups was evaluated, and the nursing students′ attitudes towards two teaching methods were investigated. Results The emergency ability was higher in the experiment group than that of the control group (P<0.05). The satisfaction level with teaching method in the experiment group was significantly higher than that of the control group (P<0.05). Conclusions SimMan human body simulator is effective in improving emergency ability of nursing students in nursing teaching experiments of emergency and critical care. It is worth popularizing and applying.
8.The effect of recombinant human erythropoietin on the function of brain mitochondria after brain injury
Zhian ZHU ; Dongfu FENG ; Yanbin MA ; Hui WU ; Xiaoyan SHEN ; Yingfan XIONG ; Fangfang WU ; Shenghua CHU
Chinese Journal of Emergency Medicine 2008;17(5):503-506
Objective To investigate the therapeutic effects of recombinant human erythropoietin(rhEPO)on brain mitochondrial energy metabolism and mitachondrial respiratory functionin after brain injury in rats.Method A total of 63 Sprague-Dawley rats were divided randomly into three groups:the rhEPO treated group(n =28),the control group(n=28),the shanl group(n=7).The models of contusion of brain caused by freefalling were set up in rhEPO treated group(n=28).The recombinant human erythropoietin was intraperitoneally injected in dose of 10 U/g immediately after brain injury and it was repeated every 10 hours in rhEPO group treated.The same models of contused brain were made without rhEPO treatment as control group(n=28).In control group,the same volume of normal saline was used in replacemem of rhEPO.Aburr hole was made on the skull of the sham group(n=7),but the brain tissue was not wounded.The mitochondria were isolated at 6 h,12 h,24h,48 h after trealment,respectively.The activity of ATPase and SOD,the content MDA and brain mitochondrial respiratory function were measured by biochemical technique.The data were analyzed with the F-test and t-test.Results The activity of ATPase(P<0.05),SOD(P<0.01)and brain mitochondrial respiratory function(P <0.05)were increased.and the levd of MDA in brain mitochondria was reduced markedly in rats treated with rhEPO.Conclusions Treatment with rhEPO can alleviates the secondary brain injury by affecting mitochondrial function.
9.Correlation analysis between psychological counseling behaviors of accompanying family members and anxiety and depression of inpatients with hepatocellular carcinoma
Yuqun WEI ; Anhua LI ; Yingfan HUANG ; Xinhua ZHAO ; Juan TANG ; Zhangyang MO ; Yonghui PANG
Chinese Journal of Practical Nursing 2017;33(8):602-607
Objective To understand the correlation between psychological counseling behaviors of accompanying family members and anxiety and depression of inpatients with hepatocellular carcinoma. Methods Used self-designed questionnaires to investigate the behaviors of 45 accompanying family members psychological counseling for their relative hospitalized patients with hepatocellular carcinoma, at the same time, evaluated the anxiety and depression among those 45 hospitalized patients with hepatocellular carcinoma, then analyzed the correlation between the accompanying family members' psychological counseling behaviors and hepatocellular carcinoma in patients' anxiety and depression. Results Among the accompanying family members,53.3%(24/45) were the spouses of the patients. During the accompanying nursing time, there were 20.0%(9/45) accompanying families told to their relative patients less than 2 hours per day, and 80.0%(36/45) told to the patients over 2 hours per day. The proportion of anxiety and depression in the hospitalized patients with hepatocellular carcinoma was 28.9%(13/45) and 77.8% (34/45) respectively. Whether the relationship between the accompanying family members and the patients with hepatocellular carcinoma were spouse relationship was related to the incidence of depression in the patients (χ2=10.470, P=0.005), and whether the time accompanying family members spent in talking with the patients over 2 hours every day was correlative with the incidence of anxiety and depression in the patients (χ2=7.012, P =0.030 andχ2=6.438, P=0.040). The patients who accompanying families told to them less than 2 hours per day suffered a higher depression rate (8/9), and among the patients who accompanying families told to them over 2 hours per day, the lowest rate of anxiety (1/12) was found in the patients who accompanying families told to them around 4.1-6.0 hours per day. Conclusions The patients who are accompanied and cared by their spouses have lower depression rate and lesser depression extent. The accompanying families talk to the patients total 4.1-6.0 hours per day could most favorably reduce the incidence of anxiety and depression in the hospitalized patients with hepatocellular carcinoma.
10.Pharmacokinetic-pharmacodynamic study on antipyreticeffects of coptisine on endotoxin-induced pyrexia of rats
Li WANG ; Yingfan HU ; Dong TONG ; Fang ZUO ; Zhicheng WEI ; Xianli MENG ; Ping WANG
Chinese Pharmacological Bulletin 2017;33(4):552-556
Aim To establish the pharmacokinetic-pharmacodynamic(PK-PD) modeling to characterize the antipyretic effects of coptisine, an active component in coptis chinensis on rats.Methods Nine healthy male Sprague-Dawley(SD) rats were randomly divided into three groups, each with three.The rats in the first group were injected intravenously with lipopolysaccharide(LPS,100 μg·kg-1) alone.The second and third group rats were given coptisine high-dose(3.87 mg·kg-1) and coptisine low-dose(1.93 mg·kg-1) by tail vein injection at 30 min after LPS injection, respectively.Body temperature was measured at different time points, and blood samples from tail vein were collected simultaneously.The blood concentration of coptisine was determined by ultra performance liquid chromatography.Monolix software was used to model PK-PD of coptisine mean plasma concentration and temperature effects,by population computation with non-covariates.Besides.the model with advantage was selected by the fitting goodness.Results Coptisine could inhibit body temperature of endotoxin-induced fever in rats significantly.Two-compartment linear elimination model was used to describe the final PK model.Gaussian function, an input function of body temperature changes, which was used to depict PD model, the PK and PD models were connected by the Emax model.At last, the final model was fitted better;the fitting results indicated that the EC50 of antipyretic effect of coptisine was 89.7 μg·L-1, and the Emax was 1.88℃.Conclusions Coptisine has a powerful anti-pyretic effect on endotoxin-induced pyrexia of rats with high potency, Low in vivo distribution and quick clearance.