Intestine is one of the fastest cellular renewal tissues and organs in the body cells,and intestinal mucosal epithelial cells keep self-renewal constantly all the life.Its effect relys on stem cells continued proliferation,differentiation and replaces the outer terminal differentiated cells.Under the stimulation after injury,the repairment of stem cells is initiated,the proliferation and differentiation of intestinal stem cells are stimulated throughout molecular mechanisms.Studys found that intestinal stem cells involve in the repairment of the gut during stress,trauma,anoxia,radioactive damage,inflammatory bowel disease and short bowel syndrome.In this paper,we briefly overviewed the definition,location,and differentiated regulation stem cells,and influence factors in the intestinal repairment.

Objective To explore the impact of curcumin on antistreptolysin“O”(ASO)and interleukin 8 (IL-8)level changes in serum of psoriatic mice model induced by phorbol ester(TPA).Methods 132 SCID mice were selected and divided into three groups.43 mice in normal group, 46 mice in model group,and 43 mice in intervention group.The mice of model group and intervention group were set up psoriasis model induced by TPA. Mice of the three groups were bred in the same environment,curcumin was injected in the intervention group mice,0.02 g/100 g,once a day,two weeks for a period of treatment.ASO and IL-8 level were compared amorg the three groups of mice after treatment.Results Before the treatment,compared with the normal group,positive rate of ASO in model group and intervention group increased significantly,and the difference was statistically significant (P<0.05 );after treatment,the level of intervention group was significantly lower than model group,and the difference was statistically significant (P<0.05 );before the treatment,compared with the normal group,IL-8 level in intervention group and model group increased significantly,and the difference was statistically significant (P<0.05 );after treatment,level of the intervention group was significantly lower than model group,and the difference was statistically significant (P<0.05).Conclusion Curcumin could effectively reduce ASO and IL-8 level in serum of psoriatic mice model, improve the clinical symptoms of psoriasis mice,it is an effective drug for treatment of psoriasis.

One of the human GM-CSF cDNA was successfully isolated with a new screening method. Up 1/170 of positive rate was reached. The COS-7 cells were transfected with the cDNA clone and the resulting supernants contained colony-stimulatory activities. Human GM-CSF cDNA was also subcloned into a expression plasmid pKPL-2D and the unfused recombinant human GM-CSF was expressed in E. coli. The expressed products could maintain in vitro the growth of a cell line BMU- 2 and stimulate its colony formation. In addition, it could increase the colony formation of bone marrow cells from the patients with acute non-lymphatic leukemia (ANLL).

Objective To establish the optimum preparation process for Yinxingye Dropping Pills. Methods The preparation conditions, such as different kinds of primary substance, cooling agent, the proportion between the extracts and primary substance, etc, were studied with L9 (34) orthogonal design. Results At the following conditions could the best was 30 d/min, dropping distance was 8cm, and cooling temperature was 10℃. Conclusion This preparation process was suitable for both laboratory test and industrial production for Yinxingye Dropping Pills.

Objective To establish the optimal extraction technology for Yishen Jiangu Granules.Methods The orthogonal test was employed for selecting the optimum of extraction technology. The investigation factors were the consumption of water, the times and time of extraction. The extraction technology of Yishen Jiangu Granules was screened by the transfer rate of icariin in herb epimedii and yield of extraction.Results The best extraction condition was as follows:10 times of water, extracted for 3 times , each time was 3 h,2 h and 2 h. Conclusion This extracting technology for Yishen Jiangu Granule was objective, feasible, stable,reasonable and with good reproducibility.

The ISO9000 family standards developed by the international organization are the international quality standards which are widely used in various types and sizes of organizations.At present,the graduate student management method introduced by our country plays a certain positive role in guaranteeing the graduate students quality,but it still lacks effective quality control and has not formed the perfect quality management system.Through the analysis of the status quo of the development of graduate education and the problems need to pay attention to in the ISO9000 family standard procedure,document and other factors as well as the construction of the ISO9000 family standard,we put forward goal elaboration,regular staff training and other measures.

Objective:To compare the content of polydatin and emodin between polygonum cuspidatum pieces and dispensing gran-ules to provide theoretical basis for the improvement of preparation process of the granules. Methods:An HPLC method with linear gra-dient elution was used to determine the content of polydatin and emodin in the pieces and dispensing granules, and the difference in the two contents was compared. Results: The content of polydatin and emodin in polygonum cuspidatum pieces met the requirement de-scribed in China pharmacopoeia, which was lower than that in the dispensing granules, and the contents of the two components in the commercial dispensing granules were not equivalent to the labeled amount, which was 1/15 and 1/27 of that in the pieces. Conclu-sion:The transport rate of the traditional preparation process of dispensing granules is with poor efficiency, which should be improved.

Objective To explore the role of LGR5 positive intestinal stem cells in repairing damage of intestinal mucosa resulting from endotoxemia and to determine whether the damaged intestinal mucosa can be repaired by regulating the proliferation and differentiation of intestinal stem cells.Methods Wistar rats were randomly divided into control group,lipopolysaccharide(LPS) group,and glucagon-like peptides 2(GLP-2) group.LPS was injected interaperitoneally into rats of the LPS group and GLP-2 group at a dose of 5 mg/kg(1 ml/kg) ; saline(1 ml/kg) was injected into the rats of control group.GLP-2 250 μg/kg(1 ml/kg) was rnjected into the rats of GLP-2 group 1 hour after the LPS injection.The terminal ilea were collected from 8 rats in each group at 6 h,24 h,and 72 h post-LPS injection.Structrral changes in the intestinal epthelium of every group were observed under the light microscope and electron microscope.The expression of LGR5 in intestinal stem cell was detected by immunohistochemical method and RT-PCR.Results In general,intestinal edema and hyperemia was observed in both LPS and GLP-2 groups at 6 h.Fracture and lodging of villi,infiltration of inflammatory cells,and visible exudate within the cavity were observed under light microscopy.The inflammatory injury was less severe in the GLP-2 group than in the LPS group at 6 h.The injury of intestina mucosa gradually repaired between 24 h and 72 h after injection of LPS in both LPS group and GLP-2 group.Importantly,samples taken from GLP-2 group and LPS group at the same time point indicated that the GLP-2 group recovered significantly better than the LPS group.And expressions of LGR5 mRNA in GLP-2 group at 6h,24h,72 h(0.13 ±0.05,0.16±0.05,0.16±0.04) were significantly higher than those in LPS group respectively (0.52 ±0.09,0.73 ±0.14,0.48 ±0.24),as shown by RT-PCR,and than that in control group(0.12 ±0.03) (P ＜ 0.05).Conclusion Exogenous GLP-2 may facilitate intestinal stem cell proliferation,thereby promoting the recovery of intestinal mucosa damaged by endotoxemia.GLP-2 appears to promote stem cell proliferation,differentiation,and gradual migration of stem cells from the intestinal crypts to the damaged mucosa.

OBJECTIVE:To study the direct powder compression technology of Angelica micropowder. METHODS: The formula of Angelica micropowder was optimized by mixture uniform design with tablet weight variation and friability as restrictive conditions, and a regression equation was established based on the comprehensive grading on the hardness and disintegration to figure out the optimal solution. DPS software was applied in the calculation. RESULTS: Prepared by the direct powder compression technology, the optimal formula of Angelica micropowder was obtained as follows: the hardness of the optimized tablet could reach as high as 75 N and the disintegrating time was about 10.24 min, with smooth and intact surface. CONCLUSION:The technology is reasonable in formulation and satisfactory in molding, and it can serve as theoretical basis for the production of Angelica tablets.

Objective To observe the clinical efficacy of stuck-needle pulling needling method in treating typeⅠgastroptosis.Method Fifty patients with typeⅠgastroptosis were randomized into a treatment group and a control group, 25cases each. The control group was intervened by ordinary acupuncture, and the treatment group was intervened by stuck-needle pulling needling at Zhongwan (CV12) in addition to the treatment given to the control group. Result The total effective rate was 96.0% in the treatment group versus 80.0% in the control group, and the total effective rate of the treatment group was significantly superior to that of the control group (P<0.01); the markedly effective rate was 88.0% in the treatment group versus 40.0% in the control group, and the markedly effective rate of the treatment group was higher than that of the control group; the treatment and control groups both showed significant efficacies in mitigating the gastroptosis symptoms (P<0.01).ConclusionStuck-needle pulling needling method can effectively manage the symptoms of typeⅠgastroptosis.