The activation of oncogenes occured in most tumors,and is believed to play an important role in tumorigenesis. However,the activation of oncogenes could induce cellular senescence in wild type cells,known as oncogene-induced senescence (OIS). Thus,the activation of oncogenes has the dual role in inducing cellular senescence or tumorigenesis. DNA damage checkpoint response (DDR) is the molecular signal transduction pathway that delay or arrest cell cycle progression in response to DNA damage and is an important molecular mechanism that induce cellular senescence. Activation of oncogenes could produce DNA damage signals and initiate DDR,which subsequently induce cellular senescence. However,when DDR pathway is deficient,activation of oncogenes could induce unlimited DNA hyper-replication and cellular hyper-proliferation,which results in accumulation of genome instability,and tumorigenesis ultimately. Therefore,the dual role of activated oncogenes is regulated by the integrity of DDR pathway. The key role of DDR in regulating activated oncogenes indicates that maintain or restore the integrity of DDR pathway might provide a new strategy in cancer prevention and therapy.

Objective To describe the Reverdin technique for treatment of hallux valgus (HV) based on correction of proximal articular set angle (PASA). Methods Thirty-one Reverdin osteotomies (in 31 feet of 17 female patients)were performed between 1992 and 1998. Reverdin operation is unique among the surgical procedures for its ability to correct large PASA with a medial wedge-type osteotomy of the metatarsal head. Results On the follow-up of 1 to 8 years, patients rated outcomes as excellent or good in 96％ (30 feet). There was no complication of delayed union or avascular necrosis of the metatarsal head. Radiographical follow-up: PASA averaged 19 degrees(range 30-12 degrees), had a postoperative averaged value of 1.95 degrees, HV angles averaged 30 degrees(range 41-23 degrees), had a postoperative value of 14.3 degrees. Intermetatarsal angles averaged 10.9 degrees (range 16-4 degrees), had a postoperative value of 5.8 degrees. Conclusion The preoperative PASA value is the most important factor in hallux valgus evaluation, as well as the selection of operative techniques. The normalization of PASA safegurds more successes and less recurrences.

Objective To investigate the clinical effect of insulin therapy in the treatment of patients with polycystic ovary syndrome .Methods 91 cases of endocrine disorders of patients with polycystic ovary syndrome were selected from September 2013 to September 2016 in Yinzhou Ningbo people’s hospital,and divided into observation group and control group,44 cases in the control group and received conventional treatment,47 cases in the observation group and received insulin sensitizer on the basis of conventional treatment.Before and after treatment, the level of sex hormones, blood glucose and insulin levels in the two groups were detected and compared,and the efficacy was determined and compared.Results Compared with before treatment,the levels of LH,FSH,E2,T,A2,Ds in the observation group were decreased,SHBG was increased,HOMA-β,HOMA-IR,FPG,FINS were decreased,the difference was statistically significant (P<0.05);compared with control group, the levels of LH,FSH,E2,T,A2,Ds in the observation group were lower,SHBG was higher,HOMA-β,HOMA-IR,FPG,FINS were lower,the difference was statistically significant (P<0.05);After treatment, the total effective rate of observation group 95.74%was significantly higher than the control group 81.82%(P<0.05).Conclusion Insulin sensitizer in treatment of polycystic ovary syndrome in patients with endocrine disorders has significantly effect,can significantly improve the patient’s sex hormone levels and blood glucose levels,adjust the patient’s insulin levels,improve the clinical treatment efficiency.

Objective To evaluate the effect and mechanism of ginseng polysaccharides injective solu-tion on invasive ability of human breast cancer MCF-7 cells.Methods Human breast cancer MCF-7 cells were treated with different concentrations of ginseng polysaccharides injections(12.5μL/mL,25μL/mL,50μL/mL or 100μL/mL),and phosphate buffer saline was served as control.The invasive ability,transfer ability and protein expression of matrix metalloproteinases-2 ( MMP-2 ) and matrix metalloproteinases-9 ( MMP-9 ) were determined via transwell matrigel invasive test,scratch test and Western Blot,respectively.Results Ginseng pol-ysaccharide injection on invasive ability and transfer ability in vitro of MCF-7 cell had significant role in inhibi-ting effect,and showed dose and time dependent.After ginseng polysaccharides injection treatment,the MMP-2 and MMP-9 protein expression significantly reduced,and showed dose and time dependent manner.Conclusion Ginseng polysaccharides injection is able to inhibit the invasion and transfer in MCF -7 cells.Moreover,theregulation of MMP -2 and MMP -9 expression may be involved in this effect.

Objective To evaluate Meta-analysis in assessing the overall diagnostic value of adenosine stress echocardiography (ASE) in patients with coronary heart disease (CHD). Methods Relevant English and Chinese articles about ASE for assessing diagnostic accuracy of CHD were collected from the literatures published from 1994 to 2007 in China and abroad. Meta-analysis was conducted with the method recommended by the Cochrane Collaboration. Results Twelve papers were included. The reported sensitivity of ASE for the diagnosis of CHD ranged from 0.69 to 0.94, and the specificity ranged from 0.71 to 1.00. The pooled weighted sensitivity and specificity was 0.81 (95%CI 0.77-0.85) and 0.83 (95%CI 0.78-0.87), respectively;and the pooled positive and negative likelihood ratio was 4.81 (95%CI 3.65-6.33) and 0.22 (95%CI 0.18-0.28), respectively. The area under the curve of the summary receiver operating characteristic was 0.8968±0.0151. Conclusion ASE is medium to highly accurate in the diagnosis of CHD.

Objective:To evaluate the changes in the transverse dimension and the perimeters of the maxillary archs produced by 3 self-ligating appliance systems during the leveling and aligning phases. Methods: 45 patients who had class I skeletal pattern and upper arch crowding 3-6 mm were divided into 3 groups with 15 subjects in every group. The dental casts were analyzed. Results: Statistically significant increases were recorded of all dentoalveolar widths (with the exception of intermolar width measured lingually) and arch perimeters. The increase in arch perimeter showed a significant negative relationship with the individual perimeter of the maxillary arch before treatment. No significant change was found in arch depth. Conclusion: The self-ligating appliance systems produce statistically significant increases in the transverse dentoalveolar width and the perimeter of the maxillary arch during the leveling and aligning phases of treatment.

Objective To explore the feature of clinical and laboratory and therapy in childern with idiopathic pulmonary hemosiderlsis(IPH).Methods The clinical and laboratory datas of 50 cases with IPH were studied from Jan.1996 to Jan.2006.The hemogram,the metabolism of ferrum,the cellular test of bone marrow,finding the hemosiderin cells in sputum or gastric juice or bronchovesicular juice and ma-nifestation were analyzed.Results IPH were often found in children aged 6 to 14 years old.Coungh and spitting blood and anemia were the most important clinical feature.X-ray manifestations were various.The diagnosis of IPH was based on clinical feature and finding out the he-mosiderin cells in sputum or gastric juice or bronchovesicular juice.X-ray and CT manifestations were important.At the same time,the secon-dary IPH was removed.The clinical symptom of IPH were relieved by using adrenal cortical hormone.Conclusions There is no specificity in children with IPH.X-ray,CT manifestations and finding the hemosiderin cells in sputum or gastric juice are helpful in diagnosis.Using adrenal cortical hormone will release symptom and prognosis.

Objective:To investigate the effects of alpha-lipoic acid on urinary albumin excretion in the patients with early diabetic nephropathy (DN). Methods:Totally 103 patients with early DN were randomly divided into the control group (n=59) and the treat-ment group ( n=44 ) . The two groups were both treated with dietary control and hypoglycemic drugs. The patients in the treatment group were given alpha-lipoic acid 450 mg·d-1 by intravenous infusion for 10 days on the basis of the initial therapy. The levels of HbA1c, BP, lipids, urinary 8-OHdG, urinary MCP-1 and urinary albumin were determined. Results:Compared with those in the con-trol group, urinary 8-OHdG, MCP-1 and urinary albumin were decreased significantly after alpha-lipoic acid treatment. The changes in HbA1c, BP and lipids were not significant. Conclusion:Aalpha-lipoic acid can decrease urinary albumin and 8-OHdG by lowering the expression of ROS and MCP-1.

Neonatal inherited metabolic diseases (IMD) screening has been widely conducted worldwide. Tandem mass spectrum (MS/MS) is the main procedure of IMD screening. As a new technique, gene sequencing has been put into practice for IMD screening. Nowadays, the morbidity and disease spectrum of IMD in China is still unclear. A summary of general and single morbidity, and disease spectrum of China's IMD from publications of MS/MS screening could provide evidence for establishing neonatal IMD's genetic test and formulation of laws and regulations.

Objective: To investigate the effects of berberine on lipids metabolism of hyper-lipidemic rabbits and to explore whether its mechanism was involved in the vitamin D receptor (VDR) gene and insulin-induced gene 2 (Insig-2) gene expressions in adipose. Methods: Forty male New Zealand white rabbits were randomly divided into five groups: normal diet (ND), high-fat diet (HFD); high-fat diet + pretreatments of either Fenofibrate (30 mg/kg) or berberine low dose (28 mg/kg, BLD), and berberine high dose (112 mg/kg, BHD) groups. The serum TC, TG, LDL-C, HDL-C, ApoA1, ApoB, and LPa in each different treated rabbit group were determined after 8-week administration. The mRNA expressions of VDR and Insig-2 in adipose tissue were quantified by RT-PCR. Results: Compared with normal diet group, the serum TC, TG, LDL-C, ApoB, and LPa in high fat diet group obviously elevated (P < 0.05), while ApoA1 obviously descend (P < 0.05). Compared with the high fat diet group, the levels of serum TC, TG, LDL-C, ApoB, and LPa in berberine treated group were significantly decreased (P < 0.05), while ApoA1 was elevated obviously (P < 0.05). The result of RT-PCR demonstrated that the mRNA expressions of VDR and Insig-2 in high fat diet group were significantly higher than those of the normal diet group (P < 0.05), and the VDR and Insig-2 mRNA expressions were significantly up-regulated by berberine (P < 0.05), especially in BLD. Conclusion: Berberine could significantly decrease the levels of blood lipids of the hyperlipidemic rabbits, the mechanism may be related to elevating the expressions of VDR and Insig-2 gene.