OBJECTIVE:To observe the effect of Tongxinluo capsule on the hemorheology of patients with coronary heart disease(CHD). METHODS:120 patients with CHD were randomly divided into 2 groups:60 case in the control group were as-signed to receive combined therapy plus Compound danshen tablets (3 tablets/time,tid),and 60 cases in treatment group to receive additional Tongxinluo capsules(3 capsules/ time,tid) in addition to the therapy applied for the control group. All patients were treated for 4 weeks during this period of time the lipid-lowering drugs and anticoagulants were discontinued. RESULT:The decreasing rates of hemorheology indexes were superior in the treatment group than in control group(P

Objective:To investigate the efficacy of dipeptidyl peptidase 4 inhibitor combined with insulin detemir (ID) in the treatment of type 2 diabetes mellitus with poor blood glucose control and its effects on blood glucose and oxidative stress indicators.Methods:A total of 105 patients with poor blood glucose control after taking oral hypoglycemic drugs who received treatment between August 2017 and September 2019 were included in this study. They were randomly assigned to receive treatment with either ID and compound vitamins (control group, n = 53) or dipeptidyl peptidase 4 inhibitor sitagliptin and ID (study group, n = 52). After 3 months of treatment, the clinical efficacy was compared between the control and study groups. Before and after 3 months of treatment, blood glucose indexes [fasting plasma glucose, 2-hour postprandial blood glucose], glycosylated hemoglobin, islet β-cell function [homeostasis model assessment-beta and homeostasis model assessment of insulin resistance)], oxidative stress index [superoxide dismutase, glutathione peroxidase, lipid hydroperoxides, malondialdehyde were compared between the two groups. The incidence of hypoglycemia was also recorded in each group. Results:After 3 months of treatment, total effective rate in the study group was significantly higher than that in the control group [92.31% (48/52) vs. 77.36% (41/53), χ2 = 4.541, P < 0.05]. After 3 months of treatment, fasting plasma glucose, 2-hour postprandial blood glucose, glycosylated hemoglobin, homeostasis model assessment of insulin resistance, lipid hydroperoxide and malondialdehyde levels in each group were significantly decreased, homeostasis model assessment-beta, superoxide dismutase and glutathione peroxidase levels were significantly increased compared with before treatment (all P < 0.05). There were significant significances in these indexes measured concurrently between the study and control groups ( t = 2.902, 3.539, 5.215, 7.313, 6.269, 4.475, 6.050, 3.323, 5.375, all P < 0.05). During the treatment, the incidence of hypoglycemia in the study group was significantly lower than that in the control group [0.00% (0/52) vs. 11.54% (6/53), χ2 = 4.319, P < 0.05]. Conclusion:Dipeptidyl peptidase 4 inhibitor combined with insulin detemir is highly effective for type 2 diabetes mellitus with poor blood glucose control. It can effectively control blood glucose level, improve oxidative stress reaction and is highly safe. Therefore, this treatment method is of good application value.

The microvasculature of the rectus abdominis muscle in dogs was observed under scanning electron microscope. The specimens were treated by vascular corrosion east and enzymic digestive methods. Some new results had been obtained.The intramuscular arterioles penetrate the muscular fasciculus with almost right angle. The capillaries go along the myofibers oriented parallel to each other and arrange fascicularly. The morphologically distinct precapillary sphincters are apparent at the original region of the capillaries. There are abundant anastomosing channels between the capillaries which constitute ladder-like microvascular networks encircling the myofibers. Sometimes, the calibre was partial enlarged in the anastomosing channel, which may be beneficial to store of the blood oxygen.The physiological significance of the intramuscular microvasculature was discussed.

At present, the prevalence of heart failure (HF) is still high in China, and its mortality and readmission rate have no downward trend. Therefore, the mechanism of its occurrence and development needs to be explored. In recent years, it has been found that about 13%-15% of HF patients have subclinical hypothyroidism (SCH), which can lead to cardiac systolic and diastolic dysfunction, thus promoting the occurrence and development of HF. At present, the curative effect of patients with mild elevation of thyroid stimulating hormone (TSH) level in HF patients with SCH is not clear, and a large number of prospective studies are still needed. In addition, in the complications of HF, SCH is the only disease that can reverse cardiac function after treatment. Therefore, this paper summarizes the mechanism of SCH in the occurrence and development of HF, and provides the basis for the formulation of clinical HF treatment strategies and the evaluation of prognosis.

OBJECTIVE: To investigate the statistical method of tri-allelic patterns of STR loci. METHODS: The DNAs of venous blood and blood stain samples from 8,846 unrelated individuals were extracted using magnetic bead method, and STR genotypes were determined by multiplex fluorescent amplification and capillary electrophoresis and analyzed using GeneMapper ID v3.2 software. The genotype frequency and allele frequency of trizonal were determined by direct counting and formula, respectively, in order to deduce the formula of trizonal in paternity test and individual recognition. RESULTS: Of the 8,846 individuals, four tri-alleles and three tri-genotypes were detected. The multiplying of allele frequency and actual rate showed significantly statistical difference. The formula of trizonal in paternity test and individual recognition was successfully deduced. CONCLUSION The frequency of the two alleles inherited as a whole in the population could be calculated by multiplying the frequencies of each allele in the tri-allelic patterns.
Alleles ; Gene Frequency ; Genetic Loci ; Genetics, Population ; Genotype ; Humans ; Microsatellite Repeats ; Polymorphism, Genetic

ObjectiveTo understand dangerous factors causing child cerebral palsy and try to diagnose and treat cerebral palsy early, and decrease the ratio of cripple.MethodsTo analyze if the occurrence, style or complication of cerebral palsy having a relation to dangerous factors such as sexes, birth weight, premature delivery, twins, neonatal asphyxia, infection of newborn, nuclear icterus, mother's age, virus infection in the duration of pregnancy, threatened abortion and family history of cerebral palsy.ResultsThe ration of male and female is 1.47∶1 in 146 cases of child cerebral palsy. Factors causing cerebral palsy are low birth weight (79.45%), premature delivery (64.38%), neonatal asphyxia (41.78%), threatened abortion (32.89%), twins (31.53%), infection of newborn (12.33%), nuclear icterus (6.16%), parturient with advanced age (3.42%), having infection history in the duration of pregnancy (2.74%), and family history of cerebral palsy (0.68%). Both styles and complications of cerebral palsy have a relation to dangerous factors.ConclusionsThe low birth weight, premature delivery, neonatal asphyxia, twins and threatened abortion all are higher dangerous factors causing child cerebral palsy. In order to abstain the occurrence of these dangerous factors, it is important to educate people and doctors recognizing all dangerous factors. It is also helpful to lower the ratio of cripple that regularly examining child with dangerous factors, discovering symptoms and complications of cerebral palsy in time, and diagnosing and treating cerebral palsy as soon as possible.

Objective: To study on in vitro release of tetanus toxoid contained in polylactide microsp heres and the influence of additives on the drug release. Methods: Some parameters affecting the rate of release were asse ssed during the in vitro experiments lasted 84 days: (1)the molecular mass of the poly mers , (2)the protein loading of the microspheres, (3)the particle sizes, (4)co-enca p sulation of additives. Results: The polylactide microspheres con taining tetanus toxoid presented considerable sustained release effect. Release kinetics of the microspheres fitted in with zero order equations. The rate of release was shown to dep end on the four parameters mentioned above. Conclusion: The poly lactide microsph eres investigated may be prospective for the development of controlled release v accines.

Objective Delivery of oxygen in tissues is limited in the space where oxygen must diffuse between the vascular and the surrounding tissues. Normal capillaries are relatively straight and well spaced, in contrast, the tumor vascular networks usually display more irregularity and the vessel wall shows higher permeability and less elasticity. The purpose of this study was to investigate the effect of capillary elasticity and tortuosity on the oxygen distribution and make further investigation on the mechanism of the formation of hypoxic regions in tumor. Method One-dimensional capillary model was coupled with the oxygen diffusion model. Oxygen transport was investigated in a Krogh and tortuous tissue model. The capillarygeometrywas obtained bythe one-dimensional model and transferred to the tissue model. Finite element method was employed in the analysis. Result The capillary radii along the flow direction under pressures were obtained for different initial radii and the oxygen distribution in the Krogh cylinder tissue model and the model with a tortuous capillary were computed. Conclusions when the capillary radius is small, the effect of vessel elasticity may have not significant effect on the oxygen distribution. However, with the capillary radius increasing, the effect on the oxygen transport becomes obvious. Moreover, with the tortuosity of the capillary increasing, the oxygen distribution becomes more heterogeneous, which is in agreement with the result in available reference. This work will be helpful to the investigation of oxygen transport within tumor.

Objective To prepare mouse anti-human monoclonal antibodies against B7-H6 and to identify their biological characteristics. Methods The B7-H6 gene was cloned by RT-PCR from a human lung adenocarcinoma cell line ( A549 ) and then subcloned into the eukaryote expression vector pCMV3 to construct the recombinant vector pCMV3-B7-H6. The recombinant vector pCMV3-B7-H6 that was verified with enzyme digestion and gene sequencing was transfected into NIH/3T3 cells by electroporation. BALB/c mice were immunized with the successfully transfected cells named 2H8 through intraperitoneal injection. The monoclonal antibodies against human B7-H6 with the advantages of high affinity and specificity were pre-pared by using hybridoma technology. Western blot assay and flow cytometry analysis were used to identify the specificity of prepared monoclonal antibodies. Results The recombinant eukaryotic expression vector encoding B7-H6 was successfully constructed. Two hybridoma clones that stably secreted monoclonal anti-bodies against B7-H6 were screened out by using flow cytometry analysis and the monoclonal antibodies se-creted by them were belonged to IgG2a isotype. Specific reactions between B7-H6 and the secreted mono-clonal antibodies were confirmed by Western blot assay and flow cytometry analysis. Conclusion The mon-oclonal antibodies which recognized B7-H6 specifically were prepared successfully.

OBJECTIVE To observe the efficiency of new low-temperature sterilization method using(hydrogen) peroxide gas plasma sterilization system.METHODS The hydrogen peroxide gas plasma sterilization system of STERRAD 100S was monitored for 157 sterilization cycles.RESULTS From them 134 cycles were(completed,) 23 cycles were(cancelled.) All cycles were completed with negative results either in biological culture or in chemical indicators.CONCLUSIONS The STERRAD Sterilization System involves the combined use of hydrogen peroxide and low-temperature gas plasma to sterilize most medical devices and materials rapidly and safely without leaving any toxic residues.As nursing develops,the new sterilization system is efficient and convenient.However,it also has own limitations.Therefore,it is desirable to fully understand the normal procedure of system according to the operation instruction.