Objective:To investigate the effects of modified Yunpi Huatan Decoction on obese patients with polycystic ovary syndrome(PCOS).Methods:60 Cases of PCOS were divided into treatment group with 30 patients and control group with 30 patients randomly.Treatment group was given modified Yunpi Huatan Decoction and the control group was given Metformin.Both had treatment period of three months.To observe the improving of menstruation,level of hormone,BMI,WHR and index change of glucose and lipid metabolism.Results:After three months,treatment group was superior to the control group in improving menstruation(P

Objective To study the effect of lecithin pollen on hyperlipidemia model experimental animals.Methods Mice were feed with high-fat feeds in order to establish the hyperlipidemia models.The contents of TC,TG and HDL-C in the serum of the mice were tested before the experiment and the mice were divided into 5 groups according to their TC level,induding the control group,the model group and 1.25,2.50and 3.75g/kg lecithin pollen groups.Lecithin pollen was given preventively for 30 days,and then the TC,TG and HDL-C level of the mice were retested.Results Compared with the model group, the TC level of the middle and high dose group decreased. The TG level of the high dose group also decreased and the HDL-C level of the high dose group increased. The difference was significant. Conclusion The lecithin pollen is effective in preventing the formation of hyperlipidemia in the experimental mice.

Objective To establish the method of assaying total flavonoids in Lecithin Pollen Pellet.Methods Suo-shi pheresis was wsed to detect wave length of 500nm with Eldrin being as control article and sodium nitrite-aluminum nitrate-sodium hydroxide as developer.Results There was good linear correlation between Absorbability A and Eldrin mass concentration C rangmg from 0 to 48?g/ml;Average recovery rate of spotting was 98.7%(RSD=1.41%).Conclusion With the character of handling simply,accurate result and good reproduction quality,the method was a scientific method for quality control of assaying effective composition Lecithin Pollen Pellet.

Objective: To explore whether short hairpin RNA (shRNA) targeting Bcl-2 can enhance the inhibitory effect of methotrexate (MTX) on growth of subcutaneously-transplanted human lymphoma in nude mice. Methods: Recombinant shRNA expression vector targeting the coding region of Bcl-2 mRNA was constructed and preserved in our lab. Human lymphoma Raji cells were injected subcutaneously into 45 nude mice to establish lymphoma models. The polyethylenimine (PEI)/shRNA complex and (or) MTX were injected into tumors. The influence of Bcl-2 shRNA and (or) MTX on tumor growth was observed. The animals were sacrificed 21 days after administration of drugs and the tumors were removed and weighed; the tumor inhibitory rate was calculated. H-E staining was used to observe the pathological morphology of the tumor. The expression of Bcl-2 mRNA in the tumor tissues was examined by RT-PCR. Results: The tumor growth was significantly slower in Bcl-2 shRNA/MTX group than in Bcl-2 shRNA or MTX alone groups (P<0.05). The tumor weight of mice in Bcl-2 shRNA plus MTX group was significantly lower than those in negative shRNA and blank plasmid group (P<0.05). The inhibition rate of tumor growth in Bcl-2 shRNA/MTX was significantly higher than those in the Bcl-2 shRNA or MTX alone groups (P< 0.05). H-E staining showed obvious apoptosis and necrosis in Bcl-2 shRNA group and MTX group. RT-PCR result showed that the expression of Bcl-2 mRNA in tumor cell suspension was significantly decreased in Bcl-2 shRNA group (P<0.05), and kept unchanged in the control group. Conclusion: The shRNA targeting Bcl-2 can enhance the inhibitory effect of MTX on the growth of subcutaneously transplanted human lymphoma in nude mice.

OBJECTIVEThis study was aimed to explore the physiological changes and the effect of heat acclimation training via a randomized control trial study.

METHODSForty healthy male volunteers were chosen and divided into experimental group and control group randomly. Those in experimental group received heat acclimation training including but not limited to meditation, unarmed run, yoga, and stepping in hot lab environment. And then, subjective feeling, rectal temperature, average skin temperature, and sweat electrolytes concentration were detected in order to describe their physiological changes. Before and after the training, both groups received some tests and their 3 000 m run-race time, nervous reaction time and subjective perception scores were recorded to evaluate the effect of acclimation training.

RESULTS(1) There was no difference in 3 000 m between the 2 groups in the same environment. Subjects' 3 000 m race time in experimental group was obviously shortened than that in control group in room temperature environment (t = 2.326, P < 0.05). And subjects' 3 000 m race time in experimental group was obviously shortened than that in control group in hot-humid environment (t = 4.518, P < 0.01). (2) Subjects' reaction time (RT) in experimental group was shortened than that in control group in room temperature environment (Z = 11.258, P < 0.05). And Subjects' RT in experimental group was sharply shortened than that in control group in hot-humid environment (Z = 6.519, P < 0.01). (3) No difference between the experimental and control groups was observed in subjective perception score (SPS) in room temperature environment. But subjects' SPS in experimental group was obviously lowered than that in control group and in hot-humid environment (t = 17.958, P < 0.01).(4) Anal temperature (AT) was lowered during training, while the change of mean skin temperature (MST) was not significant. Sweat sodium concentration (SSC) was lowered during training. SPS continued to decrease and entered plateau on the 13th day after training.(5) After acclimation training, the working capacity of the experimental group in hot-humid environment was over 85% of that in room temperature environment. While subjects' working capacity in control group in hot-humid environment was about 80% of that in room temperature environment.

CONCLUSIONHot-humid environment acclimation training improved the working capacity. After training, subjects' working capacity in hot-humid environment remained over 85% of that in room temperature environment, which was higher than that of those subjects who did not take part in training.

Acclimatization ; physiology ; Body Temperature ; Heart Rate ; Hot Temperature ; Humans ; Humidity ; Male ; Physical Conditioning, Human ; physiology ; Sweating

Child ; Humans ; Practice Guidelines as Topic ; Syncope ; diagnosis

Dental Implantation ; Dental Scaling ; Diabetes Mellitus, Type 2 ; complications ; Guided Tissue Regeneration, Periodontal ; Humans ; Male ; Middle Aged ; Periodontitis ; diagnostic imaging ; etiology ; therapy ; Radiography, Panoramic

Objective: To study the chemical constituents of Quercus pannosa. Methods: The compounds were isolated and purified by chromatography on silica gel and RP C18 columns, and HPLC, and their structures were assigned on the basic of spectroscopic data. Results: From the ethyl acetate fraction of 95% ethanol extract from Q. pannosa, eight compounds were isolated. Among them, four were lignans and other four were triterpenoids. They were identified as rel-(7α, 8β)-3-methoxy-4', 7-epoxy-8, 3'-oxyneolignan-4, 9, 8'-triol (1), rel-(7α, 8β)-3-methoxy-4', 7-epoxy-8, 3'-oxyneolignan-4, 9, 9'-triol (2), (+)-syringaresinol (3), (8R, 8'R)-4, 4'-dihydroxy-3, 3'-dimethoxylianane-7, 7'-dione (4), ursolic acid (5), oleanolic acid (6), maslinic acid (7), and hederagenin (8). Conclusion: Compound 1 is a new lignan, named quercus pannosa-triol. The other compounds are isolated from this plant for the first time.

Objective To evaluate the perfectibility of colposcopy directed biopsy for the diagnosis of cervical intraepithelial(CIN)through cervial conization by loop electrosurgical excision procedure(LEEP).Methods Biopsy by colposcopy and biopsy after LEEP were analyzed retrospectively in 108 cases of cervical intraepithelial neoplasia to study the related factors of affecting the coincidence rate.Results Comparison of pathological hanges following both LEEP and colposcopy showed that 69 cases were the same,24 upgrade,and 15 downgrade.Conclusion LEEP is a kind of effective and perfect method for the specific diagnosis and treatment of CIN.Colposcopically directed biopsy has its limitations.

Aim To study the effects of sodium ferulate on A?induced cognitive deficits and expressions of IL-1? and phospho-p38MAPK proteins.Methods Alzheimers disease model of rats was produced by intracerebroventricular injection of A?_(25-35)(10 ?g,once).Morris water maze was used to measure spatial memory performance.Nissl staining and immunohistochemical technique for glial fibrillary acidic protein(GFAP) were employed to determine the morphology of pyramidal neurons and astrocyte infiltration in hippocmpal CA1 regions.The levels of phospho-p38MAPK and IL-1? were determined by Western blot and ELISA method.Reverse transcription-PCR analysis showed changes in FasL mRNA.Results Intracerebroventricular injection of A?_(25-35)in rats resulted in spatial memory impairments shown by longer escape latency and decreased percentage of time spent in the target quadrant.These behavioral dysfunctions were accompanied by astrocyte activation and infiltration,increased IL-1? production and elevated FasL mRNA level,the loss of pyramidal neurons in hippocampal CA1,and the increase of phosphorylated p38MAPK.Oral administration of sodium ferulate(50,100,250 mg?kg~(-1)daily) and ibuprofen 15 mg?kg~(-1)daily markedly improved the memory impairment,attenuated pyramidal neuronal damage,and reversed the A?-induced increases in IL-1? and p38MAPK activation.Conclusion sodium ferulate prevents A?-induced neurotoxicity through suppressions of inflammatory response and the activation of p38MAPK.