Cell Differentiation ; drug effects ; Cells, Cultured ; Culture Media, Conditioned ; Fetal Blood ; cytology ; Hepatocytes ; cytology ; drug effects ; Humans ; Stem Cells ; cytology ; drug effects

Adenocarcinoma ; complications ; metabolism ; pathology ; Adult ; Carcinoembryonic Antigen ; metabolism ; Diagnosis, Differential ; Female ; Humans ; Immunohistochemistry ; Ki-67 Antigen ; metabolism ; Ovarian Neoplasms ; complications ; metabolism ; pathology ; Peutz-Jeghers Syndrome ; complications ; metabolism ; pathology ; Proliferating Cell Nuclear Antigen ; metabolism ; Sex Cord-Gonadal Stromal Tumors ; complications ; metabolism ; pathology ; Uterine Cervical Neoplasms ; complications ; metabolism ; pathology

Objective To study the safety and efficiency of transurethral electrochemical therapy (ECT) on benign prostatic hyperplasia (BPH). Methods An experimental electrechomical therapy on prostate has been carred out in 12 dogs.The prostate tissue and the nearly organs were studied for any morphological changes.On the basis of the experiment,ECT was then carried out for 11 patients suffered from BPH.The relevant parameters were assessed. Results In the experimental group,good therapeutic effect was achieved if the electrode was 10 mm shorter than the prostatic urethra.On the other hand if the electrode was 10 mm longer than the prostatic urethera,urethro-rectal fistula and urine incontinence might result.In the clinical group,the effective rate was 82% with no complication observed.Before the treatment RI was 0.70?0.04 and 2 weeks after treatment 0.73?0.03;IPSS 26.7?6.2 and 19.5?4.7;QOL 4.9?0.5 and 3.5?0.6;Qmax (7.8?2.0) and (12.4?3.1)ml/s;PVR (129.1?40.8) and (64.1?27.4)ml;V (48.0?13.1) and ( 47.0?13.5)ml.There was significant difference of IPSS,QOL,Qmax,PVR,RI before and after treatment (P0.05). Conclusions With proper maniputation and adequate length of the electrode used,ECT is a safe,efficient and mini-invasive means for treating BPH in high risk patients nontolerable to open surgery.

Objective · To investigate the effects of ox-LDL on the proliferation of rat theca cells and expression of LXR-α and StAR, two genes associated with androgen biosynthesis. Methods · The expression of LXR-α in the ovarian tissue of rats was determined by immunohistochemistry. Primary theca cells were isolated and collected from rat ovary and cultured in vitro. Furthermore, the theca cells were treated with 25, 50, 100, 150, 200, 300 and 400 mg/L ox-LDL, respectively. The variations in LXR-α mRNA were identified using real-time PCR. MTT assay was performed to detect cell viability. The expression of LXR-α and StAR was measured by Western blotting analysis. Results · The effect of ox-LDL on the proliferation of rat theca cells and the levels of LXR-α and StAR in theca cells was in a concentration-dependent manner. Following exposure to various concentration of ox-LDL for 24 h, the proliferation of theca cells was induced by low concentration of ox-LDL (25-150 mg/L), and 100 mg/L ox-LDL showed the most significant inducing effect. Moreover, the cell survival rate was diminished considerably following with ox-LDL concentration increasing, especially lowered by 400 mg/L ox-LDL. The mRNA level of LXR-α was increased with low concentration of ox-LDL (25-150 mg/L) and the impact of ox-LDL on the induced expression of LXR-α mRNA was considerably distinct at the concentration of 150 mg/L. On the other hand, the expression of LXR-α mRNA was reduced with high concentration of ox-LDL, and the impact of 400 mg/L ox-LDLwas substantially distinct. The protein expression levels of LXR-α and StAR were increased with 150 mg/L ox-LDL, but StAR protein level in 150 mg/L ox-LDL group revealed no significant difference when compared with control group. The expression of LXR-α and StAR protein was significantly inhibited with 400 mg/L ox-LDL in the rat theca cells. Conclusion · Low concentrations of ox-LDL can induce the proliferation of theca cells, and promote the expression of StAR and LXR-α. Whereas, high concentrations of ox-LDL can reduce the cell viability and inhibit the expression of StAR and LXR-α.

The plysical and chemical characteristics of recombinant human GM - CSF (rhGM - CSF) were studied separatly. rhGM - CSF was treated by GdHCl, reduced by DTT, and the disulfide bond was blocked by idoacetamide. The results showed that the samples aren' t homogeneous in UV absorption spectrum and RP-HPLC analysis after treatment by DTT. There was no remarkable differences in the results of the analysis of SDS-PAGE electrophoresis and western blot between treated and untreated rhGM - CSF samples. The effect of GdHCl on GM-CSF was reversible in all above tests; In peptide mapping analysis, the digestion of the samples with blocked disulfide bond by CNBr and protease is more complete than that without any treatment.

Objective To distinguish two Bithynia species,Bithynia fuchsiana and Bithynia robusta collected from Heng County,Guangxi Zhuang Autonomous Region,by using morphological and DNA barcoding methods. Methods The adult B. fuchsiana and B. robusta were collected from the biotope such as rivers,ditches and ponds in Heng County of Guangxi Zhuang Autonomous Region,China. The two species specimens were identified by measuring shell morphological parameters,compar?ing the characters of the male reproductive system,and using the COI gene barcoding technique and building phylogenetic tree. Results B. fuchsiana and B. robusta were similar morphologically in the shell appearance;they had the similar snail height, snail width,shape and male reproductive structure. The DNA sequence analysis showed that the COI gene of the two Bithynia species had low sequence divergence with 11 variation sites among 22 sequences. The length of the COI gene segment was 517 bp and no insertion sites and deletion loci after sequence edited. All individuals of the two species gathered to one clade in the phylogenetic tree based on COI gene. Conclusion According to the evidence of morphology and COI gene coding sequence,B. fuchsiana and B. robusta from Heng County,Guangxi Zhuang Autonomous Region,are likely to be the same species.

Objective This study aimed to establish an anatomic basis for the microscopic surgical anatomy of the cavemous sinus via the extended transsphenoidal approach.Methods Simulated surgery via extended transsphenoidal ap-proach was performed on seven adult cadaver heads with red--colored latex injected arteries.The cavernous segment of the ICA and its branch vessels and its relationship with cranial nerve were exposed and its anatomic parameters were measured under microscope.Results The tuberculum sellae,clivus,sellar base,ICA prominence,and optic nerve prominence are the important bone landmarks to define the surgical area.The average of the extent of bone removal of extended transsphe-noidal approach is 37.6 mm(range:28.7 mm～44.0 mm).Conclusions The bone removal from sellar base to the media edge of the foramen rotundum and over the ICA prominence can effectively expose the entire unilateral cavernous sinus.The microscopic cavernous sinus surgery via the extended transsphenoidal approach is an optimal surgical approach for the le-sions that invade the cavernous sinus from sella.

Objective:To explore the effect of stage target intervention on mental state, quality of life and prognosis of spontaneous cerebral hemorrhage, so as to provide reference for improving the quality of life and prognosis of patients with spontaneous cerebral hemorrhage.Methods:A total of 261 patients with spontaneous cerebral hemorrhage from June 2017 to June 2019 in Beijing Union Hospital were selected as the research subjects. Patients were divided into the observation group (131 cases) and the control group (130 cases) by the random number table method. The control group was treated with routine nursing intervention, and the observation group applied stage target intervention based on the control group. The psychological state, quality of life and prognosis of the two groups were evaluated by Self-rating Anxiety Scale (SAS), Self-rating Depression Scale (SDS), modified Rankin Scale (mRS), Stroke Specific Quality of Life scale (SS-QOL).Results:There were no significant differences in SAS, SDS, mRS, SS-QOL before intervention between the two groups ( P >0.05) . After intervention, the scores of energy, family role, language, activity, emotion, personality, self-care ability, social role, upper limb function, work / labor and total scores of SS-QOL in the two groups were all increased ( P < 0.05), and the increase of energy, family role, language, activity, emotion, personality, self-care ability, social role, upper limb function, work / labor and total scores of SS-QOL in the observation group were higher than those in the control group, and the differences were statistically significant ( P < 0.05). After intervention, the scores of SAS, SDS and mRS were (42.07±4.14), (43.09±4.79), (3.06±0.42) points in the observation group and (51.83±4.65), (54.82±4.92), (3.57±0.50) points in the control group, and the differences were statistically significant ( t values were 17.912, 19.516, 8.925, P < 0.05). Conclusions:Stage target intervention can improve the anxiety and depression of patients with spontaneous cerebral hemorrhage, improve the quality of life, and reduce the incidence of poor prognosis.

Objective To explore the efficacy and safety of biological mesh during female anterior pelvic organ reconstruction.Methods The 68 consecutive women with symptomatic anterior vaginal wall prolapse in Beijing hospital from January 2010 to June 2013 were divided into two groups:6 cases underwent anterior vaginal wall repair with biological mesh,and 32 cases underwent vaginal approach to paravaginal repair.Preoperative and postoperative pelvic evaluations were performed with the POP-Q system.Patients were followed up at 1,3,6,12,24 months after operation.Objective cure was defined if the lateral sulci of the anterior vaginal walls were at grade 0 and firmly apposed to the lateral pelvic sidewalls.Results Among 68 patients,38 had grade Ⅲ and 30 had grade Ⅳ anterior vaginal wall prolapse.In all patients the anterior vaginal repair was performed successfully.The indexes of operation time,amount of bleeding,the postoperative retention time of catheter and hospital stay had no difference between biological mesh and vaginal approach groups [(88.1±18.3)min vs.(88.0±17.4)min,(140.3±77.6) ml vs.(141.3±64.9) ml,(5.30±1.79) d vs.(4.90±2.34) d,(5.53±2.00)d vs.(5.50±2.08)d,t=0.01,0.05,0.64,0.06,respectively,all P＞0.05].No complication happened during and after operation.No one relapsed in biological mesh group and 4 cases relapsed in paravaginal repair group.The relapse rate between two groups is statistically different(x2 =4.79,P＜0.05).Conclusions The anterior vaginal repair with biological mesh is effective during female anterior pelvic organ reconstruction,but the long-term outcome of biological mesh in pelvic floor construction needs further study.

ObjectiveTo study specific allergic IgG antibody test and its impact factors.MethodsA total of 1770 Qingdao residents were recruited in this cross-sectional study from January to October 2011.ELISA was used to test specific allergic IgG antibodies.Logistic regression was used for multifactor analysis.Results Of 1770 participants,9 individuals had insufficient data,resulting in a response rate of 99.5％.Identification rate of specific allergic IgG antibody was 60.0％ (1056/1761 ),and the most commonly seen allergic foods were crab (49.4％ ),egg (44.80％ ),ling (38.0％ ),shrimp ( 30.4％ ) and soy ( 22.2％ ).Intolerance to crab,egg,shrimp,creamery,com,and tomato showed gender difference ( x2 =18.978,P＜0.05 ).Serum level of allergic IgG antibody was increased with age [20 - 40 years:( 118.61 ±45.67) U/ml; 41-60 years:(166.57 ±55.82) U/ml; ＞60 years:(183.67 ±49.34) U/ml; x2=13.597,P＜0.01].In logistic regression,difference between individuals with normal body weight and obesity population ( OR =1.897,95％ CI 1.215 - 2.578 ) or between those with or without allergic history ( OR =0.780,95％ CI 0.648 - 0.912) was found.Conclusions Impact factor of food intolerance includes gender,age,constitution and body mass index.Six kinds of food intolerance are more common in women.41 -60 year group shows peak of food intolerance.No exposure to allergic food and antianaphylaxis may reduce the risk of food intolerance.