Objective:To study on effect of Gutai Decoction on the abortion rate of in vitro fertilization and embryo transplantation(IVF-ET).Methods:237 cases with ?-human chorionic gonadotropin(B-HCG) ≥25IU/L 14 days after the transplantation were divided into Treatment group A (TCM administration after the transplantation),Treatment group B(TCM administration after pregnancy) and western medicine control group.Their abortion rates were observed.Results:The abortion rate was 13.08%,15% and 24% in treatment group A,B and the control group respectively,with significant difference between the treatment groups and the control group(P

Objective To approach therapy of acute lymphoblastic leukemia(ALL) complicating acute respiratory distress syndrome (ARDS) in children.Methods The therapy of seven children diagnosed as ALL complicating ARDS was analyzed, who were treated by anti-infection, methyllprednisolone, ambroxol and constant positive airway pressure (CPAP) assisted ventilation.Results Six cases were recovery and one was death. The cure rate was 85.7%. Conclusion The cure rate is high, when employing combined therapy to treat ALL complicating ARDS.

BACKGROUND:Anterior cruciate ligament injury can trigger knee instability, knee osteoarthritis which decreases human beings’ life quality. Exploration on non-contact anterior cruciate ligament injury mechanism can prevent the occurrence of anterior cruciate ligament injury.
OBJECTIVE:To investigate the mechanism of fatigue effect on non-contact anterior cruciate ligament injury and to introduce the progression of fatigue effect on non-contact anterior cruciate ligament injury in jump-landing activities.
METHODS:The author retrieved the PubMed database from 1988 to 2013 by computer. The key words were set as:ACL, landing, and fatigue. A total of 42 articles were included which related to biomechanics research on anterior cruciate ligament injury, fatigue landing and low-limb injury.
RESULTS AND CONCLUSION:In jump-landing activities, knee valgus, moment, knee joint rotation and smal er knee flexion are main biomechanics underlying non-contact anterior cruciate ligament injury. In addition, fatigue effect is another critical factor for non-contact anterior cruciate ligament injury. However, the cause-effect between fatigue effect and anterior cruciate ligament injury is not clearly investigated and summarized, especial y on fatigue level, fatigue induction and movement control. Introduction of fatigue induction is crucial for better understanding how fatigue effect results in anterior cruciate ligament injury. Thus, the quantification of fatigue level, fatigue models and landing ways wil provide new ideas for exploring biomechanical mechanism underlying anterior cruciate ligament injury in fatigue state, as wel as provide more information on intervention design and injury rehabilitation.

Objective To observe the effect of everolimus on proliferation,cycle and apoptosis of endometrial cancer cells and discuss the mechanism.Methods Proliferation of RL95-2 interfered with everolimus in different concentration and time was observed by MTT assay.Cell cycle and apoptosis affected by everolimus was detected by flow cytometry.PI3K-Akt-mTOR signaling pathway was detected by Western blot.Results (1) MTT assay showed that,cell viability in everolimus group was time-dependent decreased,compared with control group,there were significant differences in 48 h,72 h and 96 h ((64.36±4.78)％,(48.18±5.93) ％,(42.72±6.91) ％;F inner grouP =3.278,P＜ 0.05;F between groups =12.327,P＜ 0.01;F across groups=7.729,P＜0.05).Cell viability in everolimus group was dose-dependent decreased(F=3.264,P＜0.05).Compared with control group,RL95-2 cells in G1 phase and G2 phase was (69.28±2.61)％ and (4.75±0.84) ％,decreased in everolimus group,and in S phase (27.31±0.69) ％ was increased (t=5.743,P＜0.05;t =4.528,P＜0.05;t=6.209,P＜0.05).The apoptosis in everolimus group was 29.78％,higher than in control group (47.29％),the difference was significant (t =19.381,P＜0.01).(2) Western blot showed that both mTOR and p-Akt in everolimus group were dose-dependent decreased (F=3.589,P＜0.05;F =5.292,P＜0.05),Akt was dose-dependent increased (F =4.294,P＜0.05).Conclusion Everolimus decrease the cell viability and promote apoptosis via target inhibiting mTOR expression.

Objective To evaluate the efficacy and safety of mosapride in the treatment of gastroesophageal reflux disease (GERD). Methods Papers of PubMed,EMBASE,OVID,VIP Database and WANGFANG Data were searched and reviewed according to international Cochrane Collaboration.Results A total of 21 trials (1666 patients) were included.The treatment group was with mosapride and PPI,the control group was with single PPI or PPI and placebo.The Results of Meta analysis showed:for non-erosive negative reflux disease,there was no significant difference in efficacy between two groups.As for erosive esophagitis treated with mosapride (5mg):There was no significant difference in the 4-week healing rates between treatment group and control group (P =0.06).The 4-week symptom relief rate of treatment group was better than that of control group (P＜0.01).The 6-week healing rate of treatment group was better than that of control group (P＜0.01).The 6-week symptom relief rate of treatment group was better than that of control group (P＜0.01).There was no significant difference in the 8-week healing rate of esophagitis between treatment group and control group (P =0.05).The 8-week symptom relief rate of treatment group was better than that of control group (P＜0.01).For erosive esophagitis treated with mosapride 10mg,.The 8-week symptom relief rate of treatment group was better than that of control group (P=0.02).Conclusion According to the meta-analysis of these 21 trails,mosapride was safe and effective in the treatment of GERD.However due to the quality of the literatures,the above findings should be treated with caution.

Objective To investigate diagnostic value of ectopic pregnancy by transvaginal sonography (TVCDS) combining with detection of blood β-human chorionie gonadotropin(β-HCG) and progesterone(P) level. Methods 96 ectopic pregnancy patients were exahined by TVCDS,and detected β-HCG and P in the same day or next day,every two days or several days reexamination of β-HCG and P were given to the hospitalization patients. Re-suits 96 ectopic pregnancy patients including massive type 76(79.1%) ,diameter (47.6±18.4) mm;embryo sac type 20(20.9%) ,showed embryo sac structure in ectopic pregnancy area,saw pulsation in saccus vitellinus and fetus vascular color,diameter (12.86±6.85)mm. 94(97.9%) displayed Doppler signals in ectopic pregnancy area, and 89(92.7%)cases were detected low resistivity nutrient artery flow spectrum by PW, the PI average value was (0.46±0.03).96 ectopic pregnancy patients' blood β-HCG and P level were significant low(P<0.01). Conclusion TVCDS combining with detection of β-HCG and P can raise early diagnosis level of ectopie pregnancy.

Objective To evaluate the diagnostic value of computer-aided diagnosis with three-dimensional volumetric measurement in solid pulmonary nodules on follow-up CT. Methods Forty-six patients with 58 solid nodules were evaluated.Each patient underwent chest CT at least twice.The volume, doubling times(DTs) and volumetric growth rates(VGRs) of nodules were calculated with three-dimensional volumetric measurement software. If there were significant statistical differences in DTs between malignant and benign nodules, Nonparametric test was used to make the analysis.If there were significant statistical differences in upper DTs'values between malignant and benign nodules, X2 test was used to make the analysis.Final diagnosis of all nodule were confirmed by pathology or by response to diagnostic therapy or by clinical follow-up.Results The DTs of 13 malignant nodules were <400 days in 12 nodules, with a range from 45 to 465 days (median, 159 days) ; the VGRs ranged from 8% to 329% (median, 30.0%).The VGRs of 45 benign nodules ranged from -38% to 24% (median, 0%).The DTs of 24 benign nodules that increased or not changed in size were >400 days in 22 nodules, with a range from 302 days to 55 158 days (median, 2 704 days); the VGRs ranged from 0 to 24% (median, 6.5%. Twenty-one nodules that decreased in size had the VGRs ranging from -38% to -4% (median, - 16.0%), and the DTs of these nodules could not be calculated by software.There were significant statistical differences in DTs between malignant and benign nodules(P <0.05).Taking 500 days, 400 days and 300 days as the upper value for malignancies there were statistically significant differences in these values between malignant and benign nodules(P < 0.01).Their sensitivities were 100.0%, 92.3%, 76.9% respectively and specificities were 87.5%, 91.7%, 100.0% respectively.Accuracies were 91.9%, 94.6% ,91.9% respectively and positive predictive values were 8,11,0.Conclusions Taking 400 days as the upper DTs'value for malignancies has important diagnostic value. Computer-aided diagnosis with three-dimensional volumetric measurement is sensitive to the volume changes of solid pulmonary nodules on follow-up CT, and is useful in the differential diagnosis of pulmonary nodules.

ObjectiveTo evaluate percutaneous cyst in jection of aluminous mixture for renal cystic diseases.MethodsRetrospective analysis was made on 1295 cases of renal cystic diseases t reated by percutaneous cyst injection of aluminous mixture under guidance of ult rasound B.All together 1529 cysts including 1142 monocystic cysts and 153 polycy stic cysts were treated.ResultsAll the patients were f ollowed up for six months.The curative rate was 85.4%(1306/1529) and the markedl y improving rate 13.0 %(199/1529),the total effective rate was 98.4%.The lon g-term follow-up showed that reoccurence rates were 0.75%(6/798),0.92%(5/546), 1.72%(3/174) and 1.14%(1/71) in 1,3,5 and ten or more years respectively.3.1%(40 /1295) of the patients complained of lumber discomfort.No one had infection,feve r,sharp lumber pain or headache.ConclusionsCystic inje ction of aluminous mixture under the guidance of ultrasound B is a safe,feasible and effective method for the treatment of renal cystic diseases.

Objective Investigate the relation between decrease of apoptosis caused by delayed preconditioning and expression of SMAC and XIAP.Methods Sprage-Dawleyt rats were divided into four groups: control,sham,I/R and IPC/SWOP.The rats in I/R group underwent ischemia for 1 hour by classic artery ligation and reperfusion for 1 hour.The rats in IPC/SWOP group underwent tree cycles of 5-minute ischemia and 5-minute reperfusion 24 hours prior to the index occlusion.Cell apoptosis was measured by flow cytometry,the activity of caspase-3 was also measured.The expression of SMAC and XIAP in cytosol of myocardial cell was measured by Western blot.Results Cell apoptosis rate,activity of caspase-3 and expression of SMAC significantly increased in I/R as compared with control(P

【Objective】To observe the therapeutic effect of water-extract of Herba Portulacae Mixture(HPM) for genital herpes.【Methods】One hundred and twenty patients with genital herpes were equally randomized into groups A and B.Group A received water-extract of MSP,and group B received acyclovir.Seven days constituted one treatment course.The therapeutic effect was evaluated after 4 continuous treatment courses.【Results】In group A,33 were cured,13 markedly effective,11 effective,3 ineffective and the total effective rate was 95.00%;in group B,19 were cured,14 markedly effective,18 effective,9 ineffective and the total effective rate was 85.00%.The total effective rate in group A was better than that in group B(P