Perioperative nutrition support may be required when the synthesis and utilization of nutrients become disordered due to gastrointestinal abnormalities and trauma anesthesia. Proper nutrition support can reduce or prevent malnutrition, and thus facilitate the recovery and lower complications and case-fatality rate.

Objective: To analyze the chemical constituents of the essential oil extracted from the seeds of Myristica fragrans Houtt.(nutmeg) in Indonesia and to provide qualities control scientific foundations.Methods: The essential oil was extracted by steam distillation and separated with GC capillary column.The relative content of every compound was determined by normalization method and the chemical compositions were evaluated by GC-MS.Results: Ninety-three chromatographic peaks were isolated and sixty-one compounds were identified,accounting for 97.62% of the total essential oil in nutmeg.Conclusion: The essential components of nutmeg in Indonesia is monoterpenoids and it's derivatives,accounting for 78.87% of the total essential oil.And phenolic compounds were the secondary composition,accounting for 16.79%.The essential oil contains safrole,which can induce genetoxicity and mutagenicity in animal,and myristicin and elemicin which can induce illusion.So,it must be careful when Myristica fragrans Houtt was used.

Scapulohumeral periarthritis,alternate name‘lujianfeng’or‘jianlingzheng’,is a common disease among the old.Balance acupuncture is a science of modern acupuncture based on the traditional theory of Chinese medicine.In order to sum up the references regarding the clinical treatment features and experimental study of balance acupuncture on scapulohumeral periarthritis in the past few decades.The review shows that as a new modern acupuncture method,balance acupuncture treating scapulohumeral periarthritis is worth to master,popularize to develop and innovate.

Objective To study the protective effect of total glucosides of Mudan cortex(TGM) on acute myocardial ischemia in mice and its mechanism. Methods The total of 60 mice were randomly divided into 5 groups (n = 12),normal control and model control (were given equal capacity of 0.9% sodium chloride solution),and TGM at low,middle,high dose (were given with 50,100,200 mg?kg-1 TGM).The mice were administered once daily for consecutive seven days.After the last administration,the mice in the model control and drug groups were treated by intraperitoneal injection of 15 mg ? kg-1 isoproterenol to to make myocardial ischemia animal model. TGM on the T wave and J point on ECG, and serum lactate dehydrogenase (LDH),myocardial tissue superoxide dismutase (SOD),malondialdehyde (MDA) changes was detected,and the extent of myocardial ischemic injury in mice was measured by Nagar-Olsen staining. Results TGM significantly reduced the displacement of ECG T wave and J point,and improved the related biochemical indexes in mice with myocardial ischemia.The activity of LDH [(898.992± 285.108) μmol?mg-1 ] ,the content of MDA [(11.737 ±5.162) nmol?mg-1 ]in mice treated with TGM at high dose obviously decreased in comparison to the model controls,and the activity of myocardial SOD [(45. 505 ± 20.711) U?mg-1 ] significantly elevated compared with the model control.It was showed that TGM significantly diminished the areas of cardiac muscles ischemia injured via Nagar-Olsen staining. Conclusion TGM has the remarkable protective effect on acute myocardial ischemia injury in mice.

Auditory Perception ; physiology ; Child Development ; physiology ; Hearing ; physiology ; Humans ; Infant, Newborn ; growth & development ; Intensive Care Units, Neonatal ; Noise ; adverse effects

Objective To establish a method of HPLC for determining the Paeonol in Shengui Tiaojing Pill. Method Agilent Hypersil-ODS-C18 (250 mm?4.0 mm, 5 ?m) was used, the mobile phase consisted of methanol-H2O (70∶30). The flow rate was 1.0 mL/min. The detecting wave length was at 274 nm. Results The method was linear at the range of 0.052～0.84 ?g for Paeonol (r=0.999 9). The average recovery was 99.94%. Conclusion The method is simple, sensitive, accurate and can be applied to the quality control of Paeonol in Shengui Tiaojing Pill.

Objective To know the drinking water quality in villages of Tongzhou District,Beijing and provide the basic data for government. Methods 623 wells in 455 villages were sampled in 2004 and the water samples were tested with the national standard methods. Results 166 of 623 water samples were up to standard,the qualification rate was 26.65%. The drinking water quality of all villages was different,in Maqiao(58.62%) and Liyuan(76.92%) it was better than that in Xiji,Huoxian,Zhangwan,Songzhuang. The qualification rate in Xiji was only 10.26%. In the present investigation,24 water quality parameters were tested and 14 parameters exceeded the limit,such as NH3-N,iron,total bacterial count,coliform group,fluoride and arsenic. Some sanitary problems were also found. Conclusion The ground water in Tongzhou has been contaminated,especially the shallow layer ground water,more attention must be paid to improvement of drinking water quality in these villages.

Objectives To study the effect of ubiquitin proteasome inhibitor bortezomib on apoptosis and Caspase-3 expression in human umbilical arterial vascular smooth muscle cells. Methods Human umbilical artery vascular smooth muscle cells were cultured in vitro. Different doses of bortezomib (0, 4, 10, 40nmol/L) were used to treat cell. Cell proliferation activity were detected by using CCK8 method. Western Blot assay was used to measure the expression of Pro-caspase-3 and Cleaved-PARP protein. Morphology of cells in different groups are observed by confocal microscopy and apoptosis rate were detected by flow cytometry analysis. Results Bortezomib inhibits proliferation activity of vascular smooth muscle cells. With increasing of drug concentration and treating time, cell viability are reduced (P < 0.01). Cells treated with different concentrations bortezomib for 48 h showed increased apoptosis rate (P < 0.01), decreased pro-caspase-3 protein expression and increased cleaved-PARP protein expression (P < 0.01). Laser confocal microscope found that with the increase of drug concentration, the number of apoptotic cells showed an upward trend under per high-power vision. Conclusion The ubiquitin proteasome inhibitor bortezomib may decrease the expression of pro-caspase-3 and increase Cleaved-PARP expression in human umbilical artery vascular smooth muscle cells. Additionally , it could induce apoptosis in a dose-dependent manner.

There are many problems existing in humanities and social science courses in education and teaching of medical colleges and universities.Changing concepts of education,adjusting the curriculum,improving teaching methods and improving the quality of teachers are effective measures in medical colleges to strengthen the humanities and social science courses.We have made a preliminary exploration on teaching practice for humanistic education and professional education.

Objective To obtain the gene of murine Single chain Fv fragment (ScFv) against human cervical cancer and to express it in E. coli. Methods The variable region gene fragments of the heavy and light chains, which were amplified respectively using recombinant DNA techniques from CsA125 hybridoma cells, were spliced together through a flexible linker to ScFv against human cervical cancer. The ScFv genes were then cloned into expression vector pCANTAB 5E and expressed in E. coli HB2151 and TG1 respectively. The soluble ScFv were characterized by SDS PAGE and Western blot. The antigen-binding activities of the soluble and phage displayed ScFv were assayed by ELISA and cell immunohistochemical analysis. Results The expressed ScFv antibodies were soluble and phage displayed. The soluble ScFv secreted and expressed in E. coli HB2151 induced by IPTG were confirmed with SDS-PAGE, Western blot and ELISA. The specific binding capacity of the soluble and phage displayed ScFv to the surface associated antigen of human cervical cancer cell line was further confirmed with immunohistochemical studies. Conclusion The soluble and phage displayed ScFv expressed in E. coli against human cervical cancer showed high, specific affinity for the cervical cancer cell line surface associated antigen.