Objective To carry out a taxonomic identification of a strain of claviform bacteria iso-lated from prostatic fluid of a patient who suffered from chronic prostatitis, and to approach its phylogenic and biologic position. Methods We undertaked an initial identification by phenotypic characters such as morphologecal, physiological and biochemical characteristics to ascertain its phylogeny by chemical composi-tion analysis of cell wall and 16S rRNA gene sequencing and alignment. Results A club-shaped gram posi-tive rod bacillus was isolated in pure culture state. Its biochemical reactions were not active. The diamino-acid of cell wall was meso-diaminopimelic acid (meso-DAP) and it had wall chemotype Ⅳ ( contained arabi-nose, galactose and maltose ). Sequence searches of the GenBank database revealed that this strain had a highest level of 16S rDNA sequence similarity (99.4%) to C. tuberculostearicurn strain ATCC35692 with only 8 nucleotides difference. Conclusion On the basis of phenotypic and phylngenetie analysis, it is rea-sonable to assign this strain to the species C. tuberculostearicum, and this is the first isolation of C. tubercu-lostearicum from prostatic fluid home and abroad.

Objective To discovery the difference about TCM constitution between urban and rural area from the survey results performed in 2 communities of Beijing. Methods 9 standard survey scales of Chinese Medicine Constitution and four diagnostic methods of TCM were performed to identify body constitution of 161 people whose age are almost 64 years; The distribution of total constitution and the constitution distribution of same sex were further analyzed. Results The distribution of 9 TCM body constitution were different between town and country, the gentleness type distribution in country was lower than city (24.68%, 51.19%) ; Qi-depression type distribution was significant higher in country than city (44.16%,5.95%) . Conclusion Most of people in the country were yang-deficiency type and qi-depression type body constitution, the distribution of which affected by gender and age, etc.

Adult ; Aneurysm, False ; complications ; diagnosis ; Coronary Aneurysm ; complications ; diagnosis ; Coronary Angiography ; methods ; Coronary Vessels ; injuries ; Humans ; Male ; Myocardial Infarction ; etiology ; Tomography, X-Ray Computed ; methods ; Ultrasonography, Interventional ; methods

Animals ; Embryonic Stem Cells ; transplantation ; Hematopoietic Stem Cell Mobilization ; Humans ; Myocardium ; cytology ; Stem Cell Transplantation

Objective To explore whether obese adolescents had neurochemical abnormalities in frontal lobe and hippocampus.Methods Anthropometric indicators were measured in all participants.Proton magnetic resonance spectroscopy(1H-MRS)were performed in 9 obese adolescents and 9 non-obese children as controls.Spectral peaks of N-acetylaspartate(NAA),creatine(Cr),choline(Cho)of 2 cortexes were measured and their ratios were computed and compared.Results Values of NAA and Cho in frontal lobe in obese group were significantly lo-wer than those of controls.There was no significant difference of Cr,NAA/Cr,Cho/Cr in frontal lobe and hippocampus between 2 groups.Conclusion The reduced NAA and Cho on 1H-MRS suggests that amount of neural nerve and myelination of white matter may be harmed in frontal lobe of obese adolescents.

AIM: To compare the difference of A-scan and lOL Master in intraocular lens power measurement.
●METHODS:Two hundred and twenty-six patients (230 eyes) with age-related cataract were included in the study. Before surgery, axial length was measured by A-scan and lOL Master respectively and corneal curvature was measured by auto refractometer. lntraocular lens power was calculated according to the SRK-T formula. Corneal curvature was measured by auto refractometer and the refractive outcome was performed by phoropter three months after cataract surgery.
●RESULTS:The mean axial length was (23. 48 ± 1. 94) mm measured by A-scan and (23. 75±1. 96) mm measured by lOL Master. There was significant difference between them ( P < 0. 05 ). After random grouping, the preoperative and postoperative mean corneal curvature in A-scan group was (43. 94±1. 81) D and (43. 98±1. 87) D respectively. There was no statistically significant difference between them (P>0. 05). And the results were (44. 10 ± 1. 57 ) D and ( 44. 11 ± 1. 58 ) D in lOL Master group. There was no significant difference between them (P>0. 05); The mean absolute refractive error (MAE) in A-scan group was ( 0. 47 ± 0. 27 ) D and in lOL Master group (0. 41±0. 19) D. The difference was significant (P<0. 05).
●CONCLUSION: lOL Master is proved to be slightly more accurate than A-scan for lOL power calculation.

Animals ; Astragalus membranaceus ; Endotoxemia ; drug therapy ; metabolism ; Interferon-gamma ; analysis ; Interleukin-4 ; analysis ; Male ; Mice ; Phytotherapy ; Plant Extracts ; therapeutic use ; Th1 Cells ; drug effects ; metabolism ; Th2 Cells ; drug effects ; metabolism

The specific fragment of Pneumococcal surface protein A(PspA)and Pneumococcal Surface Adhesin A(PsaA)gene was amplified by PCR from Streptococcus pneumonia 5 and Streptococcus pneumonia 19.The amplified fragnent of PspA and PsaA gene was ligated into pET-27b(+)vector and transformed into BL 21 E.coli for expression and obtain the expressive production of PspA and PsaA.Induced by IPTG,the expression level was as high as 75 % of the total disolube protein.The result showed that the recombinant plasmid could express a specific 75 kDa and 37 kDa fusion protein in E.coli BL 21,which showed the good immunogenicity and a broadly cross reactivity with the other serotypes.

Objective To investigate the relationship between the level of serum uric acid(SUA) and carotid atherosclerosis in male patients with type 2 diabetes mellitus(T2DM). Methods A collection of 579 male T2DM patients with or without carotid atherosclerosis were grouped based on quartiles of SUA. Age, SUA, smokers, duration, body mass index(BMI), blood pressure, total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), blood urea nitrogen(BUN), creatinine(Cr), and HbA1C were determined in all subjects. The plaques in carotid arteries and intima-media thickness(IMT) were measured with high-resolution ultrasound. Results BMI, systolic and diastolic blood pressures, TG, and Cr showed a gradual increase, while HDL-C and HbA1C showed a gradual decrease according to the higher SUA quartiles in male T2DM(P＜0.05). Nevertheless, the detectable rates of smokers, duration, age, TC, LDL-C, and BUN had no relationships with the SUA quartiles(P＞0.05). The detectable rate of carotid atherosclerosis and the thickness of carotid plaque were positively associated with the levels of SUA in male patients with T2DM(P＜0.05). However, intima-media thickness of carotid arteries did not illustrate the correlation with the levels of SUA in male T2DM patients(P＞0.05). Age, HbA1C, and SUA were independent factors of carotid atherosclerosis in these patients by logistic regression(P＜0.05). Conclusion The levels of SUA seems to be associated with the occurrence and development of carotid atherosclerosis in male patients with T2DM.

ObjectiveTo investigate clinical risk factors and coronary angiographic features in young men with acute myocardial infarction (AMI).MethodsWe retrospectively studied 200 young men with AMI and compared with 104 non-CHD men,89 old men with AMI.All patients were admitted to hospital from January 2009 to December 2010 and undergone coronary angiography,and the clinic and coronary angiographic features were assessed.The relation between body mass index (BMI),smoking history,familial history of early coronary artery disease,essential hypertension,type 2 diabetes mellitus,hyperlipidemia,serum uric acid ( UA),lipids,hemoglobin (Hb),fasting blood glucose (FBG) with coronary artery disease and its severity was observed.The severity of CAD was diagnosed by the number of diseased vessel.Results In young men with AMI group,the factors such as smoking history,obesity,essential hypertension,type 2 diabetes mellitus,familial history of early coronary artery disease were remarkably different ( P ＜ 0.05 ) ; body mass index,fasting blood glucose,total cholesterol,triglyceride,low density lipoprotein cholesterol were higher than the control group ( P ＜ 0.05 ),hemoglobin was lower than the control group ( P ＜ 0.05 ).Applying Logistic regression analysis,obesity ( OR =11.020),type 2 diabetes mellitus ( OR =5.805 ),essential hypertension ( OR =4.428 ),familial history of early coronary artery disease ( OR =2.883 ),smoking history ( OR =2.153 ) and lower concentration hemoglobin ( OR =1.034) are independent risk factors (P ＜ 0.05) for young men with AMI. According to coronary angiography in young men with AMI,there were 14 cases with zero-vessel disease,109 cases with singlevessel disease (54.50％,69 cases involved with left anterior descending artery) and 42 cases with twovessel disease,35 cases with three-vessel disease.The ratio of single vessel disease involved left anterior desending artery in young men was higher than that of old men with AMI ( P ＜ 0.05 ).Conclusions Obesity,type 2 diabetes mellitus,essential hypertension,familial history of early coronary artery disease,smoking history and lower concentration of hemoglobin are risk factors of young men with AMI ; Single vessel coronary disease is seen more frequently in young men with AMI.