The characteristics of knowledge exchange in medical field of China were analyzed by social network analysis with the medical data covered in CNKI Citation Database as its study object, which showed that both the inflow and outflow volumes of clinical medicine are the largest and form the core in knowledge exchange network, preclinical medicine is the theoretical base of other medical subjects and the important knowledge source in medical networks, the interchange is close between traditional Chinese medicine, surgery and other clinical subjects, oncology is rather active in interdisciplinary studies, the interchange between stomatology and psychology is rather rare with other clinical subjects in knowledge networks and stomatology and psychology are two independent subjects.Carrying out information push service for these subjects by making use of their characteristics can improve the targeted information service, step up the knowledge exchange between different medical subjects, and promote the cooperative interdis-ciplinary studies.

Objective To explore the effectiveness of defluoridization of water and the morbidity trends in endemic fluoroais areas in Yanqing county of Beijing, in order to provide the basis for the formulation of prevention and control measures. Methods In 2008, stratified random was used to collect water samples to detect the amount of drinking water fluoride in three villages(Gujiaying, Bojiying and Hujiaying) in Yanqing county using ion-selective electrode in both dry and rainy seasons. Sixty-three students in 8 - 12 years old and 664 adults over 16 years of age were determined of urinary fluoride, and examine the prevalence of dental fluorosis. ResultsThe fluorine content in drinking water in the dry and rainy seasons was (0.72 ± 0.12), (0.76 ± 0.18)mg/L, respectively. The median of fluorine content in urine of children was 1.11 mg/L with the range of 0.32 - 4.15 mg/L; the median of fluorine content in urine of adult was 1.42 mg/L with the range of 0.37 - 4.65 mg/L. The detection rate of dental fluorosis in children was 23.81%(15/63), dental fluorosis index was 0.45; the detection rate of dental fluorosis in adult was 54.97% (365/664), dental fluorosis index was 1.70. Conclusions Fluorine content in drinking water meets the national standard in Yanqing county. The morbidity of endemic fluorosis descends in some extent in a result of defluoredization.

Objective To understand current status of brucellosis infection in both human and animal in Yanqing County to get solid scientific basis for controlling the disease and to prevent the spread of epidemic.Methods Cluster sampling method was used in this survey in stock raisers and ELISA and SAT were performed to inspect serologic antibody of Brucella.At the same time an epidemic investigation was carried out,including the basic personal situation,occupational history,vaccination history,clinical symptom,and so on.Resutls In a total of 216 cow raisers whose blood Was detected,27 cases showed positive in titer test of Brucella antibody with a ratio of 12.50%.However,all the antibody carriers did not have any clinical symptoms.In all 2185 cows that were collected for antibody test,1488 cases had positive serological antibody with a ratio of 68.10%.And all the positive COWS also did not have any clinical symptoms.Conclusions The subclinical infection of brucellosis is rather high in Yanqing County's stock raisers.It is self-protection of the stock raisers,epidemic knowledge propaganda,serological surveillance in human and animal,immunize of healthy cows,and eliminate of diseased cows that are urgent in order to keep the public in health.

Objectives To evaluate the diagnostic value of real-time quantitative fluorescence polymerase chain reaction( Q-RT-PCR ) assay and immunofluorescence assay for diagnosis of hMPV. Methods Totally 1 283 children with acute respiratory infection admitted in Jiaxing Maternity and Child Health Care Hospital for treatment from November 2008 to May 2009 were recruited in this study. The hMPV positive stains were separated and sequenced in this area. The sequences between the local hMPV stains and Holland stains NLD00-1 were compared. The specific primers and fluorescent probe were designed according to the sequence of epidemic hMPV strain. The Taqman methodology was applied in Q-RT-PCR. Negative pressure suction was used to acquire nasopharyngeal secretions specimens. Both Q-RT-PCR and immunofluorescence with FITC labeled monoclonal antibody were used to analyze them, respectively. The McNemar, test was applied to analyze the correlation between the two methods. Results Totally 1 283 specimens were analyzed with Q-RT-PCR and immunofiuorescence simultaneously. Q-RT-PCR analysis showed there were 59 cases positive. Immunofluorescence analysis showed there were 55 cases positive. Fifty-two cases were positive in both assays. There were 7 cases positive in Q-RT-PCR assay but negative in immunofluorescence assay and 3 cases negative in Q-RT-PCR assay but positive in immunofluorescence assay. If Q-RT-PCR method was set as the golden standard, the sensitivity and specificity for immunofluorescence detection method were 88. 1%and 99. 8%, respectively. Positive predictive value and negative predictive value were 94. 5% and 99. 4%,respectively. There was no significant difference ( χ2= 0. 9, P ＞ 0. 05 ) by McNemar' test between the two methods. Conclusion The diagnostic value of immunofluorescence assay is close to Q-RT-PCR assay.

The cell-impedance substrate sensor,which is mainly used to detect the shapes and function ofcells and other micro.organism.is a novel tool based on the technique of BIOMEMS to study the cellular physiologyand clinical Dharmasology.It has drawn attention from both domestic and international scholars as well as compa nies in recent years for its advantages such as innovative design and long-term non-invasive monitoring.This paperintroduces the interfacial model of cell-sensor in detail and gives a sketch of the recent international researchprogress.Then the novel interdigitated array,which was designed in our group recently,is introduced.The fore ground of development is discussed in the end.

Objective To investigate the common cause,inducement and treatment response in patients with status epileptic(SE) in children,in order to improve the level of prevention and treatment of the disease.Methods Valium and chloral hydrate be used in the patient untreated,followed with luminal.Deparkin injection be used in the patient uncontrolled,followed with syrup.Results Of 21 patients,3 cases with epilepsy hadn′t been treated;6 cases with epilepsy hadn′t been treated regularlly;3 cases with epilepsy place respiratory infection;4 cases were the first attack with no inducement;3 cases were suffering from viral encephalitis.Four cases with epilepsy were died who hadn′t been treated regularly.Deparkin might be helpful in treatment with SE,but poor control in patients with long time attacks.Conclusions The attack and prognosis of SE are closely relative to original sick.It is important to prevent the attack.Early diagnosis is the key factor to improve prognosis.

Objective To investigate the relationship among the proliferation of CD4+T cells, the intracellular levels of interleukin-10 (IL-10) and transforming growth factor-?1(TGF-?1), and allergic bronchial asthma. Methods Dermatophagoides farinae antigen were prepared as allergen. Twenty-five patients with asthma and 15 healthy individuals were enrolled and divided into blank control group, allergen group and self control group, respectively, after venous blood sample collection. The proliferation of CD4+T cells and the distributions of CD4+/IL-10+, CD4+/TGF-?+1 and CD4+/IL-10+/TGF-?+1 were measuredby flow cytometry (FCM). Results The distributions of CD4+/IL-10+, CD4+/TGF-?+1 and CD4+/IL-10+/TGF-?+1 could hardly be detected in the peripheral blood samples of the blank controls of the patients with asthma and healthy ones. In the allergen group of the healthy individuals, the peripheral blood CD4+T cells were significantly proliferated, and the proportions of CD4+T cells andCD4+/IL-10+ cells were much higher than the self control group, while there was no significantly increase in the proportions of CD4+/TGF-?+1 and CD4+/IL-10+/TGF-?+1 subgroups. In the allergen group of those with asthma, the proportions of peripheral blood CD4+, CD4+/IL-10+, CD4+/TGF-?+1 and CD4+/IL-10+/TGF-?+1 cells were not found significantly increased compared with those self controls. After being activated by allergen, the proportion of peripheral blood CD4+/IL-10+ cells was significantly lower in the patients with asthma than the healthy individuals(P

Objective To investigate the value of thyroid transcription factor-1(TTF-1) in the diagnosis and biological behavior assessment of hepatocellular carcinoma (HCC). Methods Thirty liver specimens obtained from benign lesions were analysed, among which 25 were hepatic cirrhosis and inflammatory diseases, and the other 5 were adenomas. And there were 176 specimens of liver tumors, among which 142 were HCC (well differentiated, n=12; moderately differentiated, n=57; poorly differentiated, n=73), 17 were intrahepatic cholangiocellular carcinoma (ICC) and the other 17 were liver metastatic carcinoma (MC). The expression of TTF-1 was examined immunohistochemically in the above tissues, and the difference in expression of TTF-1 among different tissues was examined by Fisher's exact test, Kruskal-Wallis test and Spearman rank correlation analysis. Results TTF-1 was significantly expressed in the cytoplasms of all the hepatocytes besides tumors and liver benign lesions. The expression rate of TTF-1 in HCC was 78.9% (112/142), however, TTF-1 was negatively expressed in ICC and MC(P

Objective To study on the measurement of glycyl-proline dipeptidyl aminopeptidase (GPDA) in urine and its clinical application. Methods GPDA in urine was measured by continuously monitoring method using substrate. The assay conditions and interference factors were investigated. GPDA activities in urine specimens from 334 healthy persons, 55 diabetes mellitus patients and 36 hypertension patients were measured. The differences between the kidney injured and non-kidney injured groups were analyzed. Results The linearity of the method was up to 350 U/L. The intra CV and inter CV were 1.27% and 1.33% respectively. The GPDA activity in urine specimen was stable for 7 days at 4℃. There was no interference of bilirubin, glucose, vitamins, dimethylbiguanide and gliben-clamide. No significant interference was found by hemoglobin less than 500 mg/L. GPDA level in urine specimens of 334 healthy persons were (12.9?4.2) U/g?Cr. The GPDA levels of diabetes mellitus and hypertension groups combined with kidney injured were obviously higher than that of the control group and the non-kidney injured group ( P

Acute Disease ; Adult ; Female ; Humans ; Male ; Middle Aged ; Occupational Diseases ; chemically induced ; Trimethyltin Compounds ; poisoning