Objective To investigate the expression of COX-2 in multiple myeloma(MM)and the relationship between myeloma cells proliferation and apoptosis.To provide a new prognosis factor and therapeutic target.Methods COX-2 from the 22 newly diagnosed MM,14 relapsed MM and PCNA,HSP70 of the newly diagnosed patients were detected by immunohistochemistry method.Results All the newly diagnosed MM exhibited positive COX-2 immunoreactivity.50% had strong COX-2 and 50% showed weak COX-2.Relapsed MM exhibited strong COX-2.COX-2 was related with serum β2 microglobulin,marrow plasma cells,hemoglobin,PCNA,HSP70(P=0.019,0.003,0.048,0.006,0.034).Conclusion COX-2 was overexpressed in MM.Prognosis of patients with strong COX-2 is poorer than those with weak COX-2.COX-2 may promote the proliferation and inhibit the apoptosis of myeloma cells.

Purpose To explore the ultrasonic characteristics of idiopathic retroperitoneal fibrosis (IRPF) and assess the diagnostic value of ultrasound. Materials and Methods Ultrasonic images of 11 cases of IRPF were retrospectively analyzed.Results The hypoechogenic masses encasing the abdominal aorta were detected in all cases, among which the encasement of inferior vena cava was found in 4 cases, the involvement of iliac artery in 3 cases and hydronephrosis in 9 cases.Conclusion IRPF demonstrated ultrasonic characteristics that would facilitate its detection and diagnosis.

Objective To study the clinical effect of tension-free hernia repair in inguinal emergency hernia. Methods The tension-free hernia repair was administered in 83 patients with inguinal emergency hernia.All the cases were analyzed retrospectively.Results There were two postoperative deaths in 83 cases within three days after operation.Wound infection occurred in one case.The rest recovered soon.The average stay in hospital was 5.1 days. Conclusion The tension-free hernia repair can be applied to inguinal emergency hernia with satisfactory results.

Acute Disease ; Adult ; Carbon Tetrachloride Poisoning ; diagnosis ; drug therapy ; Humans ; Male

This study was designed to discover filovirus entry inhibitors in a drug library of commercial medicines. One thousand and six hundred drugs were screened using the ZEBOV-GP/HIV model, a pseudovirus formed by an HIV-core packed with the Zaire Ebola virus glycoprotein. We identified 12 gonadal hormone drugs with inhibitory activities in ZEBOV-GP/HIV entry at final concentration of 10 μmol x L(-1). Among them, three drugs exhibited strong activities with IC50 < 1 μmol x L(-1), such as toremifene citrate (IC50: 0.19 ± 0.02 μmol x L(-1)), tamoxifen citrate (IC50: 0.32 ± 0.01 μmol x L(-1)) and clomiphene citrate (IC50: 0.53 ± 0.02 μmol x L(-1)); seven drugs had moderate activities with IC50 between 1 and 10 μmol x L(-1), such as estradiol benzoate (IC50: 1.83 ± 5.69 μmol x L(-1)), raloxifene hydrochloride (IC50: 3.48 ± 0.07 μmol x L(-1)), equilin (IC50: 4.00 ± 9.94 μmol x L(-1)), estradiol (IC50: 5.26 ± 9.92 μmol x L(-1)), quinestrol (IC50: 6.36?5.37 gmol-L1), estrone (IC50: 6.87 ± 0.03 μmol-L1) and finasteride (IC50: 9.94 ± 0.45 μmol x L(-1)); two drugs, hexestrol (IC50: 14.20 ± 0.55 μmol x L(-1)) and chlormadinone acetate (IC50: 24.60 ± 0.36 μmol x L(-1)), had weak activities against ZEBOV. Further, toremifene citrate, tamoxifen citrate, clomiphene citrate, raloxifene hydrochloride and quinestrol could block both pseudovirus type Sudan ebola virus (SEBOV-GP/HIV) and Marburg virus (MARV-GP/HIV) entries.
Antiviral Agents ; pharmacology ; Drug Evaluation, Preclinical ; Ebolavirus ; drug effects ; physiology ; Hemorrhagic Fever, Ebola ; Humans ; Marburgvirus ; drug effects ; physiology ; Selective Estrogen Receptor Modulators ; pharmacology ; Virus Internalization ; drug effects

Objective:To investigate the relationship between dopamine D1 receptor ( DRD1 ) gene polymorphisms and clozapine efficacy in male schizophrenic patients. Methods:Totally 46 male schizophrenic patients were treated by clozapine for 6-8 weeks. The clinical response was determined by the Positive and Negative Symptom Scale (PANSS). DRD1 gene rs265981, rs5326, rs4532, rs1799914, rs686 and rs4867798 polymorphisms were detected by the gene sequencing, while plasma clozapine levels were monitored during the treatment. Results:The total clinical efficacy of clozapine response group and the non-response group was compared, the distribution of rs265981 genotype TT, TC and CC and allele T and C had statistically significant differences (P=0. 025;P=0. 005), and the distribution of rs686 genotype CC, CT and TC and allele C and T had statistically significant differences ( P=0. 044;P=0. 010). The negative symptom of the response group was compared with that of the non-response group, the distribution of rs4532 gen-otype GG, GA and AA and allele G and A had statistically significant differences (P=0. 034; P=0. 013). Conclusion: The poly-morphisms of DRD1 gene rs265981 and rs686 may have influence on the clinical efficacy of clozapine, and rs4532 may have influence on the negative symptom.

Ultrasonic microbubbles were used to open blood-brain barriers (BBB) with a reversed and limited behavior feature in the study, which could improve the brain-targeted delivery of anti-tumor drugs. The glioma rat model was prepared. Low-frequency ultrasound was combined with microbubbles to affect the permeability of BBB compared with the permeability of independently administered Evans blue (EB) crossing BBB. Time point and length of ultrasound were investigated whether they affect the permeability of BBB and the damage of brain tissue. The effect of the growth time of glioma on BBB permeability was explored. Only glioma had a very little impact on BBB permeability. However, ultrasonic microbubbles opened the BBB with the features of temporary, limited and reversed behavior and improved EB and magnetic resonance imaging contrast agent penetrating BBB. A length of 30 s ultrasound is appropriate for opening BBB and no damage of brain tissue. Drugs should be injected before ultrasound so that they enter into brain as BBB opening. Ultrasonic microbubbles can open BBB effectively and safely, which improve drugs penetrating BBB under proper time point and length.
Animals ; Blood-Brain Barrier ; Contrast Media ; Drug Delivery Systems ; Glioma ; drug therapy ; Magnetic Resonance Imaging ; Microbubbles ; Permeability ; Rats ; Ultrasonics

Objective To evaluate the academic status and influence of "Chinese Journal of Endemiology" in the preventive medicinal core periodicals to provide a scientific basis for further improving the quality of the journal. Methods Journal evaluation data of "Chinese Science and Technology Journal Citation Reports" (2005 -2009 periodicals) as a statistical source, twenty-eight core journals of preventive medicine as an evaluation object, a single evaluation index (such as impact factor, total cites and he cited rate) as a reference, integrated 15 journal evaluation indicators of 28 kinds of preventive medicinial core periodicals into 4 comprehensive evaluation indexes.Calculate the composite indicator score through principal component analysis method if the index's relevant eigenvalue was greater than 1 and their cumulative contribution rate was more than 80%. Then calculated the composite indicator scores of the 4 kinds of indexes, which were weighted into linear combination of the composition with its corresponding contribution. Assess the overall level and the academic quality of "Chinese Journal of Endemiology" among core journals of preventive medicine on the basis of the 4 composite score. Results Impact factors of Chinese Journal of Endemiology were 0.909, 1.237, 0.899, 0.832, 1.019 between 2005 and 2009, ranked number 4, 1, 4, 6 and 3 among the 28 core journals. Its total cited frequency respectively were 933, 1172,1101, 1267 and 1491, among the 28 kinds of core journals and ranked number 4, 4, 6, 5 and 6. Comprehensive score of "Chinese Journal of Endemiology" were 0.269, 0.371, 0.282, 0.247 and 0.602, and its rank were 7th, 5th,7th,5th and 4th in the 28 core journals. Conclusions The impact factor and citation frequency of "Chinese Journal of Endemiology" are ranked at the forefront of preventive medicinal core periodicals. More attention should be paid to academic exchanges of related areas, and to enhance the academic quality of the journal.

Objective To evaluate the academic influence of medical statistics source journals in Heilongjiang province during 2005 - 2009. MethodsJournal evaluation data of Chinese Science and Technology Journal Citation Reports(2006 - 2010 periodicals) as a statistical source, the 7 indexes(literature sources, total cites, impact factor, immediacy index, he cited rate, ratio of overseas papers, ratio of funded papers) of medical statistics source journals in Heilongjiang province during 2005 - 2009 were analyzed. Dynamic evaluation model was used to evaluate the academic influence of journals and compute impact assessment score(Ⅰ), relative impact assessment score(Ir), and rate of growth. Results There were 5 kinds of medical statistics source journals in Heilongjiang province during 2005 - 2009, they were Journal of Harbin Medical University, Chinese Journal of Endemiology, Chinese Journal of Critical Care Medicine, Chinese Journal of Preventive Veterinary Medicine,Chinese Journal of Traditional Medical Science and Technology, respectively. Year by year, the total cites,impact factor and ratio of funded papers of above mentioned journals showed increase trend, literature sources, he cited rate and ratio of overseas papers showed stable trend, immediacy index showed greater fluctuations. Ⅰ values of above periodicals were 32 279.79, 379 379.20, 251 240.26, 117 576.00, and 83 156.65, respectively. Ir value of above periodicals were 0.19, 2.20, 1.45, 0.68, and 0.48, respectively. The rates of growth of above periodicals were 0.41, - 0.07, 0.24, 1.04, and 0.87, respectively. Conclusions The development of medical statistics source journals in Heilongjiang province is in good condition, the academic influence is an overall upward trend.

Objective To explore the application of clustering analysis in the study for iodine deficiency disorders(IDD)surveillance.Methods System cluster method of average linkage was applied to study the data obtained from national IDD surveilance in 2005 and a clustering analysis' tree chart was drawn.Such data as the rates of consuming qualified iodine salt,coverage rate of iodine salt,median of iodine salt,median urinary iodine,goiter rate detemined by palpation and B-uhrasonography,Child intelligence quotient were investigated.Results Thirty-one provinces or autonomous regions including the Xinjiang Production and Construction Coms had beed assorted into three types.Tibet was claasified into the type Ⅰ;Guangdong,Qinghai,Hainan,Sinkiang were assorted into the type Ⅱ,other areas were assorted into the typeⅢ.The rates of consuming qualified iodine salt,coverage rate of iodine salt and median urinary iodine in the type Ⅰ area were 21.00%,33.20%and 96.70 μg/L,while they were 71.95%,78.50%and 145.40μg/L,respectively,all being lower than that of type Ⅲ(93.20%.97.70%and 245.20μg/L)with statistical significance(P＜0.05).11.50%of the children in the type Ⅰ area were detected by palpation to have goiter;the child intelligence quotient in three types of areas,was 77.00,95.95±4.46 and 104.59±6.77,the differences being statistical significant between any two types(P＜0.05).Conclusion Multiple areas of IDD could be assorted by clustering analysis into distinct types with different epidemic value.More attentions of the government must be paid to the type Ⅱ areas,the prevention and cure work must be enhanced in the second-class areas while it is carried on in the typeⅢareas.The results are helpful for decision making for epidemic supervisors in the government.