OBJECTIVETo study the expression difference in MicroRNA-21 (miRNA-21) levels of the plasma between the patients with coronary heart disease and the subjects without coronary artery lesions, and its clinical significance.

METHODSPlasma was obtained from the patients with coronary heart disease(trial group,56 cases) and the subjects without coronary artery lesions(control group, 10 cases), patients with coronary disease were divided into angina(AP, 39 cases) and acute myocardial infarction(AMI, 17 cases)subgroup, the contents of miRNA-21 were detected using qRT-PCR method, and the differential expression of miRNA-21 in each group was analyzed. The levels of creatine kinase isoenzyme (CK-MB), high sensitive troponin I(cTnI), B type natriuretic peptide urea (BNP), Gensini, left ventricular ejection fraction (LVEF), integral value of coronary left ventricular end diastole diameter (LV) and homocysteine (HCY) were determined and the correlation between miRNA-21 and these clinical indexes was analyzed.

RESULTSCompared with control group, there was a significant difference in expression of miRNA-21 in patients with angina and AMI (P < 0.05), and miRNA-21 expression in AMI group was much higher than that in AP group. There was statistical significance in CK, CK-MB, cTnI, Genisis integral comparison between AMI group and control group (P < 0.05). The correlation analysis showed that there was a positive correlation between expression of CK, CK-MB, cTnI and the level of circulating miRNA-21 in patients with acute myocardial infarction. And there was a negative correlation between BNP, Gensini integral, LVEF value of coronary artery, LV and circulating miRNA-21.

CONCLUSIONmiRNA-21 was significantly elevated in acute myocardial infarction subgroup than the control group. The level of miRNA-21 associates with the degree of coronary artery stenosis, and might be a potential marker for the diagnosis of acute myocardial infarction. miRNA-21 may play an important role in protecting myocardium from ischemia/reperfusion injury.

Acute Disease ; Biomarkers ; blood ; Coronary Disease ; blood ; Creatine Kinase, MB Form ; metabolism ; Humans ; MicroRNAs ; blood ; Myocardial Infarction ; blood ; Natriuretic Peptide, Brain ; metabolism ; Troponin I ; metabolism

Objective To study the pathological characteristic of VX2 hepatic tumor in rabbit and the value of ultrasound in detecting this tumor mode. Methods To establish a model of Rabbit Liver VX2 Tumor with ultrasound- guided implantation of constitution mass and ultrasounds scanning was performed at fourteen days and twentyone days after implantation. After that, all the rabbits were executed and pathological examination was performed. Results Twenty models of Rabbit Liver VX2 Tumor were successfully established. The fourteen days after implantation,18 rabbits were found tumor,tumor diameter 8. 6 ～ 11.8 mm, average diameter ( 10. 15 ± 0. 67) mm; The twenty one days after implantation,20 rabbits were found tumor, tumor diameter 13.2 ～ 18.3 mm, average diameter( 16. 13 ±1.98)mm. Ultrasound showed that the tumor in the rabbit liver was a single spherical or sphere-alike hypoechoic nudes,and there were higher blood flow signals around the nedus. In pathological examination,the tumors were exserted to the liver surface,the cross sections of the tumor were gray,the cells of the tumors were bigger than the normal liver cells,arranged into nests with fibrous tissue. Conclusion In pathological examination, the heteromorphism of VX2 tumor cell was conspicuous. The growth of VX2 hepatoma could be monitored by ultrasound effectively.

Objective To observe the curative effects of the combined therapy of Folic acid,Vitamin(Vit) B6 and Vit B12 in youth stroke associated with hyperhomocysteinemia.Methods 150 youth stroke patients with hyperhomocysteinemia were randomly devided into low dose Folic acid,Vit B6 and Vit B12 treatment group(low dose group);high dose Folic acid,Vit B6 and Vit B12 treatment group(high dose group) and control group,50 cases in each group.Low dose group was taken by the treatments of Folic acid 2.5 mg/d,Vit B6 10 mg/d,Vit B12 0.5 mg/d;high dose group was taken by the treatments of Folic acid 5 mg/d,Vit B6 30 mg/d,Vit B12 1.5 mg/d;control group was not taken by the treatments of Folic acid,Vit B6 and Vit B12.The plasma homocysteine(Hcy)concentration was detected before and after treatment.The level of plasma Hcy 0.05).No obvious side effect was found in the three groups.Conclusions It is effective in the combined therapy of Folic acid,Vit B6 and Vit B12 in youth stroke associated with hyperhomocysteinemia.The effectiv rates are same by the low dose and high dose drug.The therapy is free from side effect.

Objective To investigate the origin of bacteria in sterile packages for war readiness under natural environment in inland region.Methods Genomic DNAs from the Bacillus cereus,Micrococcus luteus and Staphylococcus cohnii ureal in sterile packages for war readiness and from air bacteria strains in war readiness warehouse were extracted,and then used as templates to amplify the 16S rRNA and 23S rRNA ITS(internal transcribed spacer) sequences by PCR.The PCR products were extracted and sequenced,and the sequences homology analysis was performed with BLAST.Results Amplification products of DNA PCR from Bacillus cereus,Micrococcus luteus and Staphylococcus cohnii ureal were about 800,1 200 and 1 000 bp respectively;The 16S rRNA-23S rRNA ITS sequences of bacterial strains in sterile packages shared 98% to 99% homology with those from the air bacteria strains in war readiness warehouse.Conclusion The bacteria in sterile packages for war readiness come from the air in war readiness warehouse under natural environment in inland region.

Objective To understand the status of depression and compare with the same age group of Chinese norms of CES-D in urban areas and analyze its potential influencing factors among male nursing undergraduates.Methods In this cross-sectional study,the CES-D and self-made questionnaire were administrated to 102 male nursing undergraduates from two schools of nursing through two-stage random sampling.Results The prevalence of depression was 14.0％,and the score of nursing undergraduates was (11.00±6.37) points,which had no significant difference from the scores of the same age group of Chinese norms in urban areas.Univariate analysis showed that coming from countryside,worse interpersonal relationship,worse career prospect,getting no attention from parents had some significant association with depression.Multivariate analysis showed that worse career prospect and getting no attention from parents associated with lower CES-D scores.Condusions For male nursing undergraduates,efforts should be given from the aspects of families and career prospects to improve their psychological health condition,and reserve qualified resources for nursing profession.

Objective To study expiration time and the origin of bacteria for the sterile packages of strategic reserve between inland region and littoral under natural environment.Methods We prepared operation dressing package,apparatus package as well as dressing and apparatus mixed package with double- cotton and to be deposited in storage boxes of combat readiness warehouse under natural environment after pulsing vacuum steam sterilization.Three packages of every kind were put in a storage boxes and samples were taken from the packages and underwent bacterial culture at different time-points.Meanwhile,the bacteria strains in combat readiness warehouse' air and in the storage boxes were cultured and performed with homology analysis.Results Expiration time of sterile packages of strategic reserve was 40d in inland region whereas 24d in littoral.The major bacteria were G+ bacilli and G+ staphylococus in sterile packages of strategic reserve,combat readiness warehouse' air and storage boxes in two regions.In inland region,the 16～23SrRNA ITS sequences of bacteria strains in sterile packages shared about 97％～100％ homology with which between the air bacteria strains in combat readiness warehouse and the storage boxes whereas it was about 98％～99％ homology in littoral.Conclusions The storage environment is the most influential factor on expiration time of sterile packages of strategic reserve.The bacteria in sterile packages of strategic reserve came from the air of combat readiness warehouse.To ensure the expiration time of sterile package of strategic reserve,corresponding management methods are important guarantee which can provide new methods about management on strategic reserve.

OBJECTIVE:To determine the equilibrium solubility and the apparent partition coefficient of sulfamethazine(SM2) in a series of phosphate buffer solutions of different pH so as to provide a basic study for the exploitation of SM2 preparation.METHODS:A series of buffer solutions of different pH were prepared.The apparent solubility was determined by saturation method;Ko/w was calculated with concentration ratio of SM2 in n-octanol and water phase after partition equilibrium.RESULTS:The maximum equilibrium solubility was 1.916 g?L-1 at pH 2 and 1.375 g?L-1 at pH 9,and the maximum of apparent partition coefficient was 3.9070 at pH 8.CONCLUSION:The equilibrium solubility and apparent partition coefficient of SM2 are correlated to pH of the medium.SM2 dissolved preferably when pH6.8,but SM2 was more distributed in the lipid phase and easier to be absorbed by body when pH=3～8.

Adolescent ; Adult ; Eye Injuries ; epidemiology ; etiology ; therapy ; Facility Design and Construction ; Female ; Humans ; Male ; Middle Aged ; Occupational Exposure ; Retrospective Studies ; Visual Acuity

The JC virus is a widely infected human polyomavirus. Recent foreign researches showed that the JC virus infection is correlated with tumors of nervous system and digestive system, while, and study on the relationship between JC virus infection and gynecological tumor is seldom reported. In this study, we first establish the nucleic acid detection methods and procedures for JC virus and its highly homologous BK virus. The JC and BK viruses infection was evaluated by detect the viral DNA in samples including biopsy tissues, serum as well as urine of myoma of uterus (98 cases), cervical cancer (84 cases), endometrial cancer (40 cases) and ovarian tumor (72 cases) patients. The BK viral DNA positive rate was significantly higher in urine samples than that of blood and biopsy samples, and there is no significant difference of the BK viral DNA positive rate among all patient groups. The JC viral DNA positive rate is almost 0 in serum samples and biopsy. tissues, however, viral DNA positive rate is more than 50% in urine samples. In fibroids group, the JC viral DNA positive rate is up to 65. 3% which is significantly higher than that in other patients groups and healthy control. Further gynecological tumor associated viruses detection showed that only human papilloma virus infection is associated with cervical cancer, the herpes simplex virus, EB virus and cytomegalovirus infection is extremely low in our patient groups. No synergistic effect on gynecological tumor caused by viruses co-infection was observed. Our study showed that JC virus infection is highly related to the pathogenesis of uterine fibroids.
Adult ; Female ; Genital Neoplasms, Female ; virology ; Humans ; JC Virus ; classification ; genetics ; isolation & purification ; Middle Aged ; Polyomavirus Infections ; virology ; Tumor Virus Infections ; virology ; Young Adult

OBJECTIVETo investigate the effect of extracellular regulating kinase (ERK) signaling pathway on the secretion of gamma-aminobutyric acid (GABA) in cultured rat hippocampal neurons induced by stromal cell derived factor-1 (SDF-1).

METHODSThe hippocampal neurons of newborn SD rats were cultured and identified in vitro; the phosphorylation level of ERK1/2 was examined by Western blot; ELISA was used to detect the effect of PD98059, a ERK1/2 specific blocker on GABA secretion of cultured hippocampal neurons and Western blot were adopted to measure the protein expression levels of glutamate decarboxylase (GAD65/67) and gamma aminobutyric acid transporter (GAT); after blocking ERK1/2 signaling pathway with PD98059; RT-PCR was used to detect the mRNA expression levels of GAT-1 and GAD65 after treated with PD98059.

RESULTSThe levels of ERKl/2 phosphorylation were increased significantly by SDF1 acting on hippocampal neurons, and CX-CR4 receptor blocker AMD3100, could inhibit SDF-1 induced ERK1/2 activation; SDF-1 could inhibit the secretion of GABA in cultured hippocampal neurons, and ERK1/2 specific inhibitor PD98059, could partly reverse the inhibition of GABA secretion by SDF-1. The effects of SDF-1 on cultured hippocampal neurons was to decrease the mRNA genesis of glutamic acid decarboxylase GAD65 and GABA transporter GAT-1, besides, ERK inhibitor PD98059 could effectively flip the effect of SDF-1. The results of Western blot showed that SDF-1 could inhibit the protein expression of GAT-1 and GAD65/67 in hippocampal neurons and the inhibition of GAT-1 and GAD65/67 protein expression could be partially restored by ERK1/2 blocker.

CONCLUSIONSDF-1 acts on the CXCR4 of hippocampal neurons in vitro, and inhibits the expression of GAD by activating the ERK1/2 signaling pathway, and this may represent one possible pathway of GABA secretion inhibition.

Animals ; Blotting, Western ; Cells, Cultured ; Chemokine CXCL12 ; pharmacology ; Flavonoids ; pharmacology ; Glutamate Decarboxylase ; metabolism ; Hippocampus ; cytology ; MAP Kinase Signaling System ; Neurons ; metabolism ; Phosphorylation ; RNA, Messenger ; Rats ; Rats, Sprague-Dawley ; Receptors, CXCR4 ; metabolism ; gamma-Aminobutyric Acid ; secretion