Overdenture as a treatment modality for both partially and fully edentulous patients is costeffective and less expensive.The purpose of the present study was to examine the newly fabricated attachments by comparing them with conventional O-ring attachment in vitro in terms of retention force and cyclic aging resistance.A total of 150 samples were prepared and divided into five groups according to the materials used (O-ring attachment,Deflex M10 XR,Deflex Classic SR,Deflex Acrilato FD,and flexible acrylic resin).The retention force of different attachments was measured by a mini dental implant after three subsequent aging (0,63,and 126) cycles in the circumstances similar to the oral environment.The gap space between the head of the implant and the inner surface of the attachments was detected.Two-way analysis of variance (ANOVA) analysis with multiple comparisons test was applied for statistical analysis.The results showed that Deflex M10 XR had the highest retention force and the lowest gap space after cyclic aging;in addition,by comparing the relative force reduction,the lowest values were obtained in the O-ring attachment and the highest values in the flexible acrylic resin attachment.The retention force measured after cyclic aging for the Deflex M10 XR attachment was greatly improved when compared with the O-ring attachment and other types of attachment materials;in addition,the Deflex M10 XR attachment exhibited the minimum gap space between the inner surface and the mini dental implant head.In conclusion,Deflex M10 XR has the ability to withstand weathering conditions and retains its durable and retentive properties after aging when compared with other attachments.

Objective To explore the changes serum S-100? in children with acute carbon monoxide poisoning and its clinical significance.Methods The levels of serum S-100? of 28 children with acute carbon monoxide poisoning and those of 20 healthy children were mea-sured by enzyme-linked immunosorbent assay.Results The serum S-100? levels of the study group and control group were(0.517?0.346)and(0.037?0.014)?g/L respectively,there was significant difference between two groups(t=6.197 P

The clinical success of dental implants can be improved by achieving optimum implant properties,such as their biomechanical and surface characteristics.Nano-structured coatings can play an important role in improving the implant surface.The purpose of the present study was to determine the most appropriate conditions for electrophoretic deposition (EPD) of nano-zirconia coatings on Ti-6Al-7Nb substrates and to evaluate the structural and biomechanical characteristics of these deposited coatings on the dental implants.EPD was used with different applied voltages and time periods to obtain a uniform layer of nano-zirconia on Ti-6Al-7Nb samples.The coated samples were weighed and the thickness of the product layer was measured.Surface analysis was performed by using optical microscopical examination,scanning electron microscope and X-ray diffraction phase analysis.For in vivo examination,48 screw-designed implants (24 uncoated and 24 nano-zirconia coated) were implanted in both tibiae of 12 white New Zealand rabbits and evaluated biomechanically after 4-and 12-week healing intervals.Results revealed that the use of different conditions for EPD affected the final coating film properties.Increasing the applied voltage and coating time period increased the deposited nano-zirconia film thickness and weight.By selecting the appropriate coating conditions,and analyzing scanning electron microscopical examination and XRD patterns,this technique could produce a thin and continuous nano-zirconia layer with a uniform thickness of the Ti-6Al-7Nb samples.Mechanically,the nano-zirconia-coated implants showed a highly statistically significant difference in removal torque values,while histologically these coated implants enhanced and promoted osseointegration after 4 and 12 weeks of healing,compared with the uncoated ones.In conclusion,EPD is an effective technique for providing a high quality nano-zirconia coating film on dental implant surfaces.Moreover,the osseointegration of these coated dental implants is improved compared with that of uncoated ones.

Aim To evaluate the effects of pantoprazole on various experimental acute ulcer inrats and mice. Methods The model of a gastric ulcer of rats or mice was caused bystree- induced ulcer and ligatel pylurus-induced ulcer. Results & Conclusions At adose of 5, 10, 20 mg? kg-1 of Pantoprazole can markedly decrease the ulcer index ofstree-induced ulcer. Pantoprazole(4, 8, 16 mg? kg -1 ) significantly decrease the areaof ligated pylorus-induced gastric ulcer. It was also found that pantoprazole caninhibit the output of basic gastric acid.

OBJECTIVETo investigate the oxidative damage of ultraviolet A (UVA) to human immortalized keratinocytes line HaCaT and the protective effects of total flavonoids of Broussonetia papyrifera (TFBP) gotten from the leaves of broussonetia papyifera.

METHODSBased on the culture of the human keratinocytes, the experiment group added with different dosages of TFBP before exposure to the radiation, received the UVA radiation together with the treatment group. The levels of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were determined in cultured HaCaT cells as well as the cell activity with MTT reduction assay. Human immortalized keratinocytes HaCaT cells received ultraviolet A with the different dosages between 0.46 and 2.76 J/cm(2) respectively. The protective effects of TFBP at different concentrations were also evaluated.

RESULTSThe cell activity decreased gradually from 96.3% to 37.5% with the increase of UVA dosage from 0.46 J/cm(2) to 2.76 J/cm(2). After 10 mg/L up to 200 mg/L of TFBP were added the cell activity increased, the levels of MDA decreased from (5.14 +/- 0.58) nmol/mg pro to (2.98 +/- 0.14) nmol/mg pro, the levels of SOD increased from (23.09 +/- 3.91) U/mg pro to (34.50 +/- 1.59) U/mg pro and the activity of GSH-Px increased somewhat.

CONCLUSIONUltraviolet A causes significant oxidative injury to HaCaT cells under the conditions of this study. TFBP gotten from the leaves of broussonetia papyrifera has certain protective effect on HaCaT epithelial cells.

Antioxidants ; pharmacology ; Broussonetia ; chemistry ; Cells, Cultured ; Dose-Response Relationship, Drug ; Dose-Response Relationship, Radiation ; Flavonoids ; pharmacology ; Glutathione Peroxidase ; metabolism ; Humans ; Keratinocytes ; drug effects ; radiation effects ; Lipid Peroxidation ; drug effects ; radiation effects ; Malondialdehyde ; metabolism ; Plant Leaves ; chemistry ; Superoxide Dismutase ; metabolism ; Ultraviolet Rays ; adverse effects

OBJECTIVETo gain insight into the mechanism by which sex-determining region of Y chromosome (SRY)-related high-mobility-group box 2 (SOX2) involved in carcinogenesis and cancer stem cells (CSCs).

DATA SOURCESThe data used in this review were mainly published in English from 2000 to present obtained from PubMed. The search terms were "SOX2," "cancer," "tumor" or "CSCs."

STUDY SELECTIONArticles studying the mitochondria-related pathologic mechanism and treatment of glaucoma were selected and reviewed.

RESULTSSOX2, a transcription factor that is the key in maintaining pluripotent properties of stem cells, is a member of SRY-related high-mobility group domain proteins. SOX2 participates in many biological processes, such as modulation of cell proliferation, regulation of cell death signaling, cell apoptosis, and most importantly, tumor formation and development. Although SOX2 has been implicated in the biology of various tumors and CSCs, the findings are highly controversial, and information regarding the underlying mechanism remains limited. Moreover, the mechanism by which SOX2 involved in carcinogenesis and tumor progression is rather unclear yet.

CONCLUSIONSHere, we review the important biological functions of SOX2 in different tumors and CSCs, and the function of SOX2 signaling in the pathobiology of neoplasia, such as Wnt/β-catenin signaling pathway, Hippo signaling pathway, Survivin signaling pathway, PI3K/Akt signaling pathway, and so on. Targeting towards SOX2 may be an effective therapeutic strategy for cancer therapy.

Gene Expression Regulation, Neoplastic ; Humans ; Neoplasms ; metabolism ; Neoplastic Stem Cells ; metabolism ; SOXB1 Transcription Factors ; metabolism

OBJECTIVETo investigate the effects of transforming growth factor-beta1 (TGF-beta1) gene therapy on bone defect and bone rarefaction around endosseous implant.

METHODSThe primary cultured bone marrow derived stroma cells (BMSCs) was transfected by plasmid pCDNA3.1(+) -TGF-beta1, and was adhered with polylactic-co-glycolic acid (PLGA) for constructing TGF-beta1 gene-modified artificial bone. The model of rats with placed titanium implants in the proximal metaphyses of the tibiae after ovariectomy was made. The TGF-beta1 gene-modified artificial bone (experimental group), BMSCs-PLGA compound artificial bone (control group) and nothing (blank control group) were placed in the bone defect around implant. The tibiae were examined by decalcified sections with immunohistochemical method and histological analysis methods at intervals of 4 and 8 weeks after implant surgery in order to detect the expression of TGF-beta1 in new bone adjacent to the implant and the healing of the bone defect around the implant.

RESULTSThe expression level of TGF-beta1 of experimental group was higher than that of control group and blank control group at the 4th week. The histological analysis indicated that the gene-modified artificial bone had stronger osetogenic potential than others.

CONCLUSIONTGF-beta1 gene-modified artificial bone promotes the repair of the bone defect around titanium implants in osteoporotic rats.

Animals ; Bone and Bones ; Cells, Cultured ; Dental Implantation, Endosseous ; Dental Implants ; Female ; Genetic Therapy ; Prostheses and Implants ; Rats ; Stromal Cells ; Titanium ; Transfection ; Transforming Growth Factor beta1

OBJECTIVETo explore the character of inorganic elements in Asari Radix et Rhizoma (Xixin).

METHODThe contents of 53 inorganic elements in Xixin samples from different localities and species were determined by ICP-AES and ICP-MS. The statistical data were made using SAS.

RESULTThe result demonstrated that Xixin has the high contents of Fe, Cr, Li. It has been observed that the content of Cu and Pb of the samples are much higher than the standard level. The results of hierarchical cluster analysis revealed two groups which correspond with the species of the samples. No correlations between the contents of the inorganic elements and the localities of the samples were found. Some characteristic elements were displayed in some specific areas. The difference of the contents of the 53 inorganic elements between root and rhizome of Xixin was reported for the first time. The primary form of inorganic elements in Xixin has been studied for the first time. The result demonstrated that the extraction rate between different elements varied, with the average extraction rate of (22.25 +/- 24.96)%.

CONCLUSIONThe inorganic elements analysis of Xixin can provide evidence of its identification, cultivation and application.

Asarum ; chemistry ; classification ; China ; Drugs, Chinese Herbal ; analysis ; Rhizome ; chemistry ; Trace Elements ; analysis

Cadherins ; metabolism ; Female ; Humans ; MCF-7 Cells ; Neoplastic Stem Cells ; metabolism ; Receptor, Notch1 ; metabolism ; Receptors, CXCR4 ; metabolism ; Vascular Endothelial Growth Factor A ; metabolism ; Vascular Endothelial Growth Factor C ; metabolism

OBJECTIVETo compare the contents of baicalin in the raw medical material and the different prepared medical materials of Scutellaria baicalensis.

METHODThe contents of baicalin were determined by HPLC. Chromatographic conditions included Hypersil C18 column and the mobile phase consisting of a mixture of methanol-0.04% phosphoric acid (46:54). Baicalin was detected at 280 nm. The standard curve was linear in the range of 0.0280-4.6700 micrograms with correlation coefficient 1.0000. The average recovery of baicalin was 101.22% with RSD = 2.54% (n = 5).

RESULTThe contents of baicalin were 6.80% in raw material, 6.00% and 6.73% in the different prepared medical materials of S. baicalensis respectively.

CONCLUSIONThe two different kinds of preparing methods can be used to the raw material of S. baicalensis.

Chromatography, High Pressure Liquid ; Flavonoids ; analysis ; Hot Temperature ; Plants, Medicinal ; chemistry ; Scutellaria baicalensis ; chemistry ; Technology, Pharmaceutical