2.Affection of Zidan decoction containing serum on endometrial receptivity in uterine immunity environment
Qian CHEN ; Zhe JIN ; Qiudan LU ; Ying CAO ; Nan LI
International Journal of Traditional Chinese Medicine 2012;34(3):220-223
Objective To investigate the effect of Zidan decoction containing serum on endometrial receptivity in uterine immunity environment,and then to discuss if it's effect on endometrial receptivity through its controlling the expression of cell factors which related to endometrial receptivity.Methods Mice's endometrium tissue samples and uterine Natural Killer (UNK) cells were obtained from pregnant mice on gd 4.5 days and gd 12.5 days.After endometrial glandular epithelial cells,endometrial stromal cells and UNK cells were separated through primary culture,epithelial cells and endometrial stromal cells at rate of 1 ∶ 1 a certain concentration UNK were mixed together and highly purified-cultured.Different concentrations of Zidan decoction was acted on the culture solution.FBS,black and aspirin control group were setup for contrast.The expression of LIF and VEGF which influenced by Zidan decoction in the immunity environment was assayed by Realtime-PCR.Results The expression of LIF and VEGF was significantly increased in the immunity environment of uterine (in 10% Zidan decoction group,10% aspirin group and 10% blank control group:LIF was 2.10±0.20,1.98±0.14,0.90±0.05 separately,and VEGF was 3.17±0.31,2.52±0.09,0.92±0.06respectively,P<0.05).Conclusion Zidan decoction could improve endometrial receptivity by increasing the expression of cell factor which related to endometrial receptivity with the help ofUNK paracrine.
3.Preliminary exploration on model of pathology residency training program in China
Ying GUO ; Zengshan LI ; Zhe WANG ; Ruian WANG
Chinese Journal of Medical Education Research 2012;(11):1186-1188
Chinese medical student's education system is quite different from that in western countries.Besides,Chinese residency training program is just starting.The professional training of pathologists,who make the final diagnosis in the hospital,is not satisfied.The present situation in China is that overall quality of pathologists is low and varied.The pathology department of Xijing Hospital started to carry out the pathology residency training program since 2009.It is expected to search for a model of pathology residency training program with Chinese characteristics.
4.Anolysis of related factors about prognosis of patients with Severey Traumatic Brain Injury
Zhiqiang LI ; Dongqing SHEN ; Zhe QUAN ; Ming CHEN ; Ying LIU
Chinese Journal of Primary Medicine and Pharmacy 2009;16(8):1361-1362
Objective To evaluate prognostic indicators in severe traumatic brain injury and intracania hamatomas with hernia and analyze which is the most important indicator.Methods Data of 84 cases with severe traumatic brain injury were retrospectively analyze .Age,GCS,pupil reflex,midline shift,compression of the cisteme,decompression time and complex injury were considered as possible prognostic indicators.SPSS13.0 was employed to analyze the data,Logistic regression(Forward conditional)analysis was done to confirm which are the most important prognostic indicators for severe traumatic brain injury and to evaluate the practical value in predicting prognosis(X2= 22.92,P< 0.01).Results 48 patients died and 36 survived.56(67%)patients had a bad prognosis(GCS≤3)and 28(33%)had a good prognosis(GCS >3).Those who had a shorter decompression time(≤3h)had a better prognosis than those who had a longer decompression time(>3h),with the mortality rate of 11% and 67% respectively.Lesser cisteme compression predicts better outcome.GCS,pupil reflex,midline shift,and complex injury didn' t enter the logistic regression equation.According to compression time,90.5% of the cases' prognosis was accurately predicted,according to compression time and cisterne compression,95.2% was accurately predicted.Conclusion The mortality rate of severe traumatic brain injury with hernia was high and surgical intervention was effective.Decompression time and cisteme compression were the most important factors affecting prognosis in severe traumatic brain injury and they could predict prognosis of most cases correctly.
5.Twelve Cases of Hiatal Hernia in Neonates
li-ying, RONG ; zhe, LIU ; jing, CHEN ; jing, GUO
Journal of Applied Clinical Pediatrics 2006;0(23):-
Objective To make hiatal hernia(HH) children grow and develop well by making correct early diagnosis and reducing misdiagnosis.Methods Reviewing clinical data of 12 patients with HH,including clinical character,X-ray diagnosis,correct examination,treatment and prognosis.Results Of 12 patients,9 cases were left HH,and 3 cases were right HH.All of them had internal expectant treatment;4 of them had operation.All of them had a good prognosis.Conclusions X-ray radiograph is a chief method of diagnosis of hiatal hernia.It is important to choose a specific position properly during radiograph examination.
6.The tolerance of contemporaneous multiplane operations in obstructive sleep apnea-hypopnea ;syndrome
Tao JIANG ; Xianhua LI ; Na HUA ; Zhe JIN ; Ying GUO
Chinese Journal of Postgraduates of Medicine 2016;39(5):447-449
Objective To observe the tolerance of contemporaneous multiplane operations in obstructive sleep apnea-hypopnea syndrome(OSAHS). Methods Twenty-three patients were enrolled. According to the different obstructive level, the different operations were chosen to complete upper airway reconstruction in contemporaneous operation group (group A, 13 patients). In the simple palatopharynx level obstructive group (group B, 10 patients), bilateral tonsillectomy and H-UPPP was chosen. Results The operation time in group A was significantly longer than that in group B:(121.0 ± 35.4) min vs.(80.7 ± 25.3) min, P<0.01. The hospital days and adverse events in two groups had no significantly difference (P>0.05). Conclusions Individual therapeutic schedule for OSAHS patients should be formulated. Operating the different obstructive levels simutaneously, which would solve upper airway occlusion and complete the upper airway reconstruction at the same time. No more adverse events happen, compared with the simple palatopharynx level obstructive group, though the operation time may be longer.
7.Initial study on relationship between pICLn protein and hypotonic state.
Ying-zhe MA ; Gui-rong ZHANG ; Li-hua WANG
Chinese Journal of Applied Physiology 2005;21(1):24-25
Animals
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Cell Line
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Chloride Channels
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metabolism
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Epithelial Cells
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metabolism
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Escherichia coli
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metabolism
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Kidney
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cytology
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Osmotic Pressure
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Periplasm
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metabolism
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Swine
9.Modulation of autophagy in ischemic penumbra by hydroxysafflor yellow A after cerebral ischemia/reperfusion injury
Zhe GONG ; Xiaoni ZHANG ; Honghong LI ; Xiangpen LI ; Ying PENG ; Jingrui PAN
Chinese Journal of Pathophysiology 2017;33(3):449-454
AIM: To clarify the modulation of autophagy in ischemic penumbra by hydroxysafflor yellow A ( HSYA) after cerebral ischemia/reperfusion ( I/R) injury.METHODS:Male SD rats subjected to transient middle cere-bral artery occlusion for 90 min were randomly divided into 4 groups:sham-operation (sham) group, cerebral I/R (I/R) group, I/R+HSYA group and sham+HSYA group.Modified neurological severity score (mNSS) was used to evaluate the neurological deficits of the rats at 6 h, 1 d, 3 d and 7 d after reperfusion , accompanied by the detection of autophagy , apoptosis and mRNA expression of IFN-βin ischemic penumbra .RESULTS:Compared with sham group , upregulation of LC3-Ⅱand degradation of SQSTM1/P62 were observed in I/R group, indicating the activation of autophagy after I/R.The activation of autophagy in I/R+HSYA group was significantly enhanced by HSYA on day 1 and day 3, and inhibited on day 7, as compared with I/R group (P<0.05).Besides, the mRNA expression of IFN-βin I/R group was significantly upregulated at 6 h, then downregulated on day 1 and day 3, and returned to basal level on day 7, as compared with sham group (P<0.05).In I/R+HSYA group, the mRNA expression of IFN-βwas significantly upregulated on day 1 and day 3, accompanied by the inhibition of apoptosis on day 3 and the significantly decreased mNSS from day 4, as compared with I/R group (P<0.05).CONCLUSION:HSYA alleviates cerebral I/R injury by dynamically modulating the activation of autophagy and the expression of IFN-βin ischemic penumbra .
10.Videofluoroscopic swallowing study in planning dysphagia treatment
Ganghua GUO ; Zhe LI ; Chenxia GUAN ; Cining SU ; Lin YUE ; Ying LI
Chinese Journal of Physical Medicine and Rehabilitation 2010;32(1):22-25
Objective To explore the application of videofluoroscopic swallowing study (VFSS) in the treatment of dysphagia post-stroke. Methods Eighty patients were assigned into control and treatment groups. Both groups accepted routine drug treatments and physical therapy, and all patients underwent VFSS on the 1 st and 28th day of the study. The patients in the treatment group accepted weekly VFSS in addition, and their swal-lowing training schedules were formulated according to the VFSS assessment results. Water drinking tests and de-glutition disorders were adopted to assess the patients' swallowing function before and after therapy. Results In treatment group, where the therapy schedule was adjusted using VFSS every week, the adjustment proportion at the 2nd, 3rd and 4th week was 20.6% , 40.7% and 15.8% , respectively. Before treatment there was no difference between the two groups with regard to water drinking, deglutition or VFSS scores. After training the water drinking and deglutition results and the time for iodine to transit the oral cavity and pharynx all improved significantly in both groups. The improvements in the treatment group were significantly greater than in the con-trol group. Conclusions Swallowing training based on videofluoroscopic assessment can significantly alleviate post-stroke dysphagia.