Objective:To investigate the inhibition effects of different concentrations of ?-tocopherol on pancreatic islet cell viability,insulin secretion,anti-oxidation competence induced by streptozotocin. Methods:Pancreatic islet cells from Wistar rats aged 1 to 3 days were cultured in monolayer in vitro. and divided into 4 groups.Then,cell viability,insulin secretion,T-AOC,SOD activity,MDA level,NOS activity and NO2-/NO3- production were measured by differrent detection methods. Results:Streptozotocin significantly decreased cell viability,T-AOC activity,SOD activity and insulin release level(P0.05),that could not be blocked by ?-tocopherol. Conclusion:?-tocopherol can attenuate the damage of rats pancreatic islet cells induced by streptozotocin,the mechanisms of which may be that ?-tocopherol can reinforce the competence of scavenging free radicals of pancreatic islet cells and not related to NOS activity change.

OBJECTIVE:To investigate the contents of aluminium in Chinese patent medicines for sale and analyze the possible cause of it.METHODS:Three kinds of Chinese patent medicine were selected randomly,i.e.Jinkui shenqi pills,Sanshen pills and Zhuangyao jianshen pills.The contents of aluminium in 3 kinds of Chinese patent medicine were determined by spectrophotometry.RESULTS:There were different contents of aluminium among 3 kinds of Chinese patent medicine.CONCLUSIONS:Chinese patent medicines included in study contain aluminium,which is associated with the production,process and habitats of drugs and cause of ADR induced by traditional Chinese medicine.

Antibodies, Viral ; analysis ; Cerebrospinal Fluid ; virology ; Child ; Child, Preschool ; Feces ; virology ; Genotype ; Humans ; Infant ; Meningoencephalitis ; diagnosis ; virology ; Parechovirus ; classification ; genetics ; isolation & purification ; Picornaviridae Infections ; diagnosis ; epidemiology ; virology ; RNA, Viral ; genetics ; Respiratory Tract Infections ; diagnosis ; virology ; Sepsis ; diagnosis ; virology ; Sequence Analysis, DNA

The combination of chemotherapy, radiotherapy and targeted therapies are considered to be major treatments for metastatic pancreatic cancer. At present, the target therapy-related medications include mainly antagonist of epidermal growth factor receptor, monoclonal antibody of vascular endothelial growth factor and so on. Erlotinib combined with gemcitabine has been demonstrated to prolong the survival of patients with pancreatic cancer.

Cervical cancer was the most common gynecological malignant tumor, and endanger the lives of women. In recent years, research on antioxidant system confirmed that the antioxidant system of defects was closely related with the risk factors of cervical cancer. The lack of antioxidants that generates excessive reactive oxygen species, damaged the cervix cells. Human papilloma virus (HPV) infection was the main pathogenic factor of cervical cancer.Epidemiological studies had shown that including oxidative stress and antioxidant defects may prompt some auxiliary factors of HPV infection were finally for the development of cervical cancer. Because the oxidation has small side effects, safety, etc, so its anti-tumor effect was becoming more and more attention. Recently, on the antioxidant therapy in the treatment of cervical cancer research has made great achievements. This article expounds the antioxidant system defects and relationship between occurrence and development of cervical cancer, also reviewed the antioxidant therapy of cervical cancer research foundation and the latest clinical research.

The key of individualized therapy is that using molecular markers to select more suitable drug.At present,some studies have confirmed that certain molecular markers can predict the efficacy of the drugs.For example,cetuximab is beneficial in patients with K-ras wild-type colorectal cancer,gefitinib and erlotinib are beneficial in patients with epidermal growth factor receptor (EGFR) mutation-positive non-small cell lung cancer( NSCLC),imatinib is beneficial in patients with C-KIT gene-positive gastrointestinal stromal tumor.In terms of safety,the promoter polymorphism of UDP glucuronosyltransferase 1 polypeptide A1 ( UGT1 A1 ) can predict the toxicity of irinotecan.

Objective To compare the applications of half-Fourier acquisition single-shot turbo spin-echo(HASTE)sequence and true fast imaging with steady state precession(True FISP)sequence for the delineation of structures and diseases in several twin pregnancy with comorbidities and to explore the clinical value of two series of fast imaging for MRI of fetus.Methods 27 twin preg-nancy women with comorbidities were imaged with HASTE and True FISP sequences.All images were statistically analyzed in re-spect of imaging qualities and artifacts.MR signs of fetal brain were analysized.Results There were abnormal changes in brains in 7 of 27 cases.Both sequences could demonstrate the structures and lesions of the fatus.Imaging quality scores of HASTE were higher than that of True FISP,but with more respiratory blurring.Conclusion There might be varying abnormalities in central nervous system in twin pregnancy with comorbidities.Combining the demonstration abilities of HASTE and True FISP is needed when de-tecting fetus lesions,especially the brain.

Cell Differentiation ; drug effects ; Cells, Cultured ; Culture Media, Conditioned ; Fetal Blood ; cytology ; Hepatocytes ; cytology ; drug effects ; Humans ; Stem Cells ; cytology ; drug effects

Antigens, CD34 ; metabolism ; Hemangioendothelioma, Epithelioid ; metabolism ; pathology ; surgery ; Hepatectomy ; methods ; Humans ; Liver Neoplasms ; metabolism ; pathology ; surgery ; Male ; Middle Aged ; Vascular Endothelial Growth Factor A ; metabolism ; von Willebrand Factor ; metabolism

Objective: To analyze and study the effect of acupuncture on patients with different disturbance of consciousness using nonlinear dynamics of electroencephalography (EEG). Methods: Thirty-seven patients with disturbance of consciousness within six months after stroke were enrolled in the study, of those, 16 patients were in a minimally conscious state (MCS), and 21 were in a persistent vegetative state (PVS). The acupuncture points Zusanli (ST36), Sanyinjiao (SP6), Taichong (LV3), and Yongquan (KI1) were stimulated by needles. The changes of nonlinear EEG indices (including complexity [Cx] and approximate entropy [ApEn]) in different brain regions (prefrontal, frontal, central, parietal, occipital, anterior temporal, middle temporal and post-temporal regions) in both groups were compared before and during acupuncture. Results: Circled digit oneAs compared to the nonlinear EEG indices before the acupuncture, the values of Cx and ApEn in the affected brain regions in the PVS group did not change significantly when acupuncturing the affected sides; and those in some affected brain regions in the MCS group were increased (P < 0.05). Circled digit twoAs compared to the nonlinear EEG indices before acupuncture, the values of Cx and ApEn in a small number of the unaffected brain regions in the PVS group were increased when acupuncturing the unaffected sides (P < 0.05), and those in the majority of unaffected brain regions in the MCS group were increased (P < 0.05, P < 0.01). Circled digit threeBefore acupuncture, there was no significant difference about the values of Cx and ApEn in all the affected brain regions between the PVS and MCS groups; when acupuncturing the points on the affected sides, there was no significant difference about the values of Cx in all the affected brain regions between the two groups, but the values of ApEn in the prefrontal, anterior temporal, middle temporal and post-temporal regions in the MCS group were higher than those in the PVS group (P < 0.05, P < 0.01). Circled digit fourBefore acupuncture, the values of Cx and ApEn in the majority of unaffected brain regions in the MCS group were higher than those in the PVS group (P < 0.05, P < 0.01); when acupuncturing the unaffected sides, the values of Cx and ApEn in all the unaffected brain regions in the MCS group were higher than those in the PVS group (P < 0.05, P < 0.01). Conclusion: The cortical activities in patients with PVS and MCS were obviously in an inhibitory state. The degree of cortical inhibition was relatively milder in patients with MCS than that in patients with PVS. Acupuncture may induce the excitability of the less injured cerebral cortex, and be caught by EEG nonlinear analysis.