Many studies have shown that changes in ion channels,abnormal regulation of neurotransmitters and their receptors,blood-brain barrier damage,inflammation,oxidative stress etc,are all related with seizures.Animal models of epilepsy,focusing on autopsy of patients with epilepsy or pathological changes of epileptic,have found that there is a certain relationship between astrocytes and epilepsy,but the cause has remained unclear.At present,most of antiepilepfic drugs work on the target of neurons,which have certain impact on the patient's congnitive and brain function.Therefore,to investigate the pathogenesis of epilepsy from the perspective of astcytes,it might help to identify new intervention targets for new antiepileptic drugs.

Paroxysmal supraventricular tachycardia (PSVT) is one of the frequent cardiovascular diseases.In recent years, cryoablation has become a research hot spot for PSVT treatment.Theoretically, its operability and safety are both better than those of radiofrequency ablation.Cryoablation can significantly reduce risk of ablation complications, but how to reduce recurrent rate after cryoablation is still a problem in the future.The present article made a review on main therapeutic methods of PSVT, especially application of cryoablation.

Epidemiologic Methods ; Humans ; Population Surveillance ; Public Health

Objective To evaluate the methods of correction for secondary deformity after removal of polyacrylamide hydrogel in breast.Methods From June 2006 to December 2007,the Center of Breast Plastic and Reconstruction at Chinese Academy of Medical Sciences admitted and treated 36 patients who experienced deformity after polyacrylamide hydrogel remoral in breasts.The average age of the patients was 27.5 years,and the time of consultation for the correction was from 6 months postoperatively.The patients who had preoperative MRI examinations showed that no visible polyacrylamide hydrogel remained in the breast were included in the study.The patients were classified according to the deformity of the breast and the chest wall tissue.Autologous fat injection grafting,silicon gel implant augmentation,and dermis grafting were performed for treating the deformity of the breasts after polyacrylamide hydrogel removal.Results During 3 to 18 months follow-up,the shape of the breast was improved and no complications such as infection,the sclerotic nodules,implant exposure occurred.35(97.2%) patients were satisfied with the result of the operations.Conclusions The correction for secondary deformity of breast after removal of PAHG should be performed at least 6 months after removal of polyacrylamide hydrogel.The optimal and nature contour of the breast may be recovered by combination of various surgical methods which are carefully selected according to the individual situation.

A good funds budget can optimize efficiency of the research funds. Through the implementation of a National Science and Technology Major Project, the authors analysed the budget management proposed some skills and suggestions for the planning and management of budget.

OBJECTIVE To investigate the occurrence and the genotype character of AmpC ?-lactamases-producing Escherichia coli isolated from the Second Hospital affiliated to Harbin Medical University.METHODS K-B disk diffusion test and FOX(≤17mm) test were used as initial screen tests to detect the clinical isolates;AmpC ?-lactamases were confirmed by three-dimentional extract tests;multiple-PCR was used for detecting plasmid-mediated AmpC gene and their genotypes were determined by DNA sequencing;the regulator genes of high producing AmpC isolates of E.coli were cloned to pUCm vectors and sequenced,the difference among them was analyzed by blast method.RESULTS Among total 586 E.coli clinical isolates,10 isolates(1.70%) resisted to cefoxitin;three-dimensional extract tests were positive for all 10 isolates;4 isolates of E.coli were plasmid-mediated DHA-1 type AmpC ?-lactamases by using multiplex PCR and DNA sequencing;6 isolates of chromosomal high level AmpC ?-lactamases-producing E.coli were sequenced and contrasted to that of E.coli K12,showed that they were gene polymorphism.CONCLUSIONS E.coli clinical isolates producing high level AmpC ?-lactamases not only acquire plasmid-mediated DHA-1 AmpC ?-lactamases but also cause by chromosomal ampC promotor or attenuator gene mutations in our hostiptal.

Objective To select a electronic medical tag system suitable for modern war. Methods The active radio frequency identification technology was surveyed, modeled, and improved. Results It was demonstrated be tests that active radio frequency identification technology met the requirements of electronic medical tag system. Conclusion The electronic medical tag system based on radio frequency identification technology changes the traditional working mode and enhances integral capability of field first aid.

Objective To explore the impact of genetic background on pancreatic p-cell first-phase secretion function with L-arginine (L-ARG) stimulation test.Methods Plasma insulin level was detected in 201 cases before and after L-ARG stimulation test.Among them, 61 cases were newly diagnosed type 2 diabetic patients with family history of diabetes ( FH + DM ) , 55 newly diagnosed type 2 diabetic patients without family history of diabetes ( FH - DM) ,31 with normal glucose tolerance and family history of diabetes ( FH + ) 54 with normal glucose tolerance but without family history of diabetes ( FH - ).Homeostasis model assessment ( HOMA) was used to estimate insulin resistance (HOMA-IR).Results It was premised that gender, age and BMI were similar among the 4 groups.(1)TC,TG,fasting plasma glucose,2h plasma glucose,fasting insulin and HOMA-IR in the two groups of newly diagnosed type 2 diabetic patients with or without family history of diabetes were significantly higher than those in the two groups of normal glucose tolerance with or without family history of diabetes.The multiples of the peak level and the base level of insulin secretion in the groups of newly diagnosed diabetes were significantly lower than those in the groups of normal glucose tolerance with and without family history(P <0.05).(2) Insulin secretion reached a peak in 2 minutes and started to decline in 4 minutes in all the four groups.( 3 ) The multiples of the peak level and the base level of insulin secretion in normal glucose tolerance group with family history of diabetes were 20.8% lower than those in the group without family history, being 7.27 and 9.18 respectively ( P < 0.05 ).(4)Two-minute peak insulin secretion, HOMA-IR and age in the newly diagnosed type 2 diabetic group with family history of diabetes was significantly lower than these in the group without family history ( P < 0.05 ).The multiples of the peak level and the base level of insulin secretion in the newly diagnosed type 2 diabetic group with family history of diabetes and that group without family history were 5.18 and 5.31 respectively and there was no significant difference between the two groups( P >0.05).(5) When the normal glucose tolerance subjects with family history of diabetes progressed to suffer from diabetes, the multiples of the peak level and the base level of insulin secretion declined 43.6% (P < 0.05) more than those in the subjects still with normal glucose tolerance without family history.Conclusion In the early course of diabetes, insulin resistance dose not function significantly, but genetic background make the first-phase secretory function of the p-cell to decline gradually and type 2 diabetes occurs easily.In the absence of genetic background, insulin resistance makes first-phase the secretion of insulin to decline relatively slow.

0.05), respectively; the costs were 640.50yuan and 1 151.21yuan, respectively; the cost-effectiveness ratios were 7.01 and 12.28, respectively; the incremental cost-effectiveness ratio of Group B versus Group A was 220.1. CONCLUSION: The sequential therapy of levofloxacin is preferable in the treatment of lower respiratory infection.

Objective To assess the role of hip ultrasonography in the early screening and following up of developmental dysplasia of the hip (DDH). Methods A total of 1324 hips of 662 infants younger than 6 months underwent ultrasonography with Graf method. The sonographic appearances of bony roof, superior bony rim, cartilaginous roof, as well as α and β angle measurements were classified according to the Graf method. Risk factors such as gender, sides, fetal position, and swaddling used were assessed. Following up were performed with ultrasonography in the cases of immaturity, dysplasia and dislocation that needed to be monitored or treated. Results Detection rate of selective ultrasound in screening of DDH of the hip was 7.42%. Both gender and fetal position was the risk factor of DDH. Twenty-seven infants with dysplasia hip and five with dislocation hip were followed-up with ultrasonography during treatment process. Thirteen infants with dysplasia turned to normal after abduction exercises, while other fourteen received treatment in Pavlik harness for no improvement in ultrasonography. All infants of dislocation received operation eventually because of failing to Pavlik harness. Conclusion Ultrasonography can objectively assess the development of DDH, monitor the course of following-up and treatment of DDH, and serve as a main tool in the early screening and following up of DDH in infants.