Objective To explore the clinical value of scanning the initial segment of right subclavian artery routinely in carotid artery ultrasonography, thus to increase the detectable rate of arteriosclerosis.Methods 2 896 subjects who had undergone carotid artery ultrasonography were analyzed retrospectively from November 2010 to June 2015 in Shuishang Park Hospital,Tianjin.The initial segment of right subclavian artery had been examined rou-tinely in carotid artery ultrasonography from February 2013.The detectable rate of arteriosclerosis at the carotid artery was compared in the two groups.The detectable rate of arteriosclerosis at the bifurcation of common carotid artery and the initial segment of right subclavian artery was analyzed and compared in different age -groups from February 2013 to June 2015 ( group B ) .Results The detectable rate of arteriosclerosis at the carotid artery in group B (74.21%) was significantly higher than 57.34%of group A(χ2 =91.82,P=0.000).The detectable rate of arterio-sclerosis at the carotid artery in group B was increased by age ( the initial segment of right subclavian artery χ2 =181.829,P=0.000;the bifurcation of common carotid arteryχ2 =635.298,P=0.000).The detectable rate of arteri-osclerosis at the initial segment of right subclavian artery was higher than that of the bifurcation of common carotid artery in group of age 36-45 years and >45-55 years (the group of age 36-45 years χ2 =8.988,P=0.003;the group of age >45-55 yearsχ2 =19.507,P=0.000).Conclusion It is necessary that examining the initial seg-ment of right subclavian artery routinely in carotid artery ultrasonography to increase the detectable rate of arterioscle-rosis,and the initial segment of right subclavian artery is the important part to find the arteriosclerosis of carotid artery early,it is of guiding significance to prevent cardiovascular and cerebrovascular disease.

Objective To establish the method of assaying total flavonoids in Lecithin Pollen Pellet.Methods Suo-shi pheresis was wsed to detect wave length of 500nm with Eldrin being as control article and sodium nitrite-aluminum nitrate-sodium hydroxide as developer.Results There was good linear correlation between Absorbability A and Eldrin mass concentration C rangmg from 0 to 48?g/ml;Average recovery rate of spotting was 98.7%(RSD=1.41%).Conclusion With the character of handling simply,accurate result and good reproduction quality,the method was a scientific method for quality control of assaying effective composition Lecithin Pollen Pellet.

Objective To study the effect of lecithin pollen on hyperlipidemia model experimental animals.Methods Mice were feed with high-fat feeds in order to establish the hyperlipidemia models.The contents of TC,TG and HDL-C in the serum of the mice were tested before the experiment and the mice were divided into 5 groups according to their TC level,induding the control group,the model group and 1.25,2.50and 3.75g/kg lecithin pollen groups.Lecithin pollen was given preventively for 30 days,and then the TC,TG and HDL-C level of the mice were retested.Results Compared with the model group, the TC level of the middle and high dose group decreased. The TG level of the high dose group also decreased and the HDL-C level of the high dose group increased. The difference was significant. Conclusion The lecithin pollen is effective in preventing the formation of hyperlipidemia in the experimental mice.

Objective:To analyze the analgesic effectiveness of dezocine after laparoscopic cholecystectomy. Methods: Totally 80 patients with laparoscopic cholecystectomy were randomly divided into the observation group and the control group. The control group was given tramadol 0. 2 mg·kg-1 for anesthetize, the observation group was given dezocine 0. 1 mg·kg-1 analgesia. Conducted according to a simgle drug additional pain status of the patient. After the surgery, the time of surgery, VAS and BCS pain condition score of the pa-tients were analyzed and compared, and the incidence of adverse reactions of the two groups were recorded. Results:After the surgery, the operation time, opening eyes, awaking time and extubation time showed no significant difference in the two groups (P>0. 05). VAS scores of the observation group in 30min, 1h, 2h and 6h after the surgery were significantly lower than those of the control group, and BCS score was significantly higher than that of the control group (P<0. 05). The incidence of adverse reactions in the observation group was significantly lower than that in the control group (χ2 =4. 020 5,P=0. 045 0). Conclusion:The analgesic effect of dezocine after lap-aroscopic cholecystectomy is promising, which can significantly alleviate the pain after the operation and is worthy of wider application.

As two main research methods, qualitative study and quantitative study are different in theoretical basis, research methods, research goal, the relation between researchers and those who are studied as well as applied fields. They should be combined in education research, however, for education is a complicated humanism phenomenon.

Astragalus Plant ; Cell Differentiation ; drug effects ; Cell Division ; drug effects ; Codonopsis ; Drugs, Chinese Herbal ; pharmacology ; Hematopoietic Stem Cells ; cytology ; drug effects

Objective:To investigate a potential role of Toll-like receptor 4(TLR4),a pathogen pattern recognition receptor, in the early phase of severely burned rats. Methods: Rats burn model(30% of total body surface area[TBSA], III grade) were established with vapor at 108°C for 8 seconds. Rats were sacrificed before and 2,5,12,24,48 and 72 h after burning, and the spleen specimens and peripheral blood samples were harvested. TLR4 mRNA and TNF-α mRNA expression in splenocyte was measured by reverse-transcription PCR (RT-PCR); the expression of TLR4 protein were measured by Western bloting; the endothelial toxicity concentration in plasma was detected by limulus lysate test. Results: It was found that the expression of TLR4 mRNA, TNF-α mRNA, TLR4 protein, and the level of ET were significantly increased in burned group compared with normal control group. The expression of TLR4 mRNA and protein peaked at 8 h after burning, the expession of TNF-α mRNA peaked at 12 h,and the level of ET peaked at 8 h after burning; the peak values of them were (3.66±0.51),(2.27±0.19), (1.65±0.23), and (11.68±2.63) Eu/ml,respectively,all significantly higher than those of the control group(P<0.01 or P< 0.05). The expression of TLR4 mRNA showed a positive correlation with that of ET and TNF-α mRNA(r=0.811,0.462, P< 0.01). Conclusion: Severe burn can cause endotoxemia and upregulate the expression of TLR4 and TNF-α in splenocytes. The up-regulation of TLR4 might participate in the pathological procedure of excessive inflammation.

30 mmHg on day 1 after SLT.Blurring and anterior uveitis were controlled. Conclusion SLT may be a safe and effective therapy for POAG.

Objective Assess the value of several biomarkers and disease severity scores for the prognostic assessment of sepsis.Methods The clinical data of adult patients, who met the diagnostic criteria for Sepsis-3 and admitted to the intensive care unit (ICU) of Affiliated Hospital of Guizhou Medical University from January 2015 to December 2016 were retrospectively analyzed. These patients were divided into survival group and death group. The levels of serum lactate (Lac), lactate clearance rate of 24 hours later (24 h LCR), procalcitonin (PCT), quick sequential organ failure assessment (qSOFA) score, SOFA score, simplified acute physiology score Ⅱ (SAPS Ⅱ), acute physiology and chronic health evaluation scoring system Ⅱ (APACHE Ⅱ) score were determined, and the receiver operating characteristic curve (ROC) were used to analyze the prognostic value of the indicators above.Results 110 of 152 sepsis patients survived, while the others died. Compared with survival group, serum Lac, PCT, SOFA score, qSOFA score, SAPS Ⅱ score, APACHE Ⅱ score of death group were increased, and 24 h LCR was decreased. SAPS Ⅱ[area under the ROC curve (AUC) = 0.877,P = 0.000, when threshold value was 41.50, sensitivity was 94.3%, specificity was 68.5%], 24 h LCR (AUC = 0.869,P = 0.000, when threshold value was 40.2%, sensitivity was 92.1%, specificity was 75.5%) and SOFA score (AUC = 0.815,P = 0.000, when threshold value was 7.60, sensitivity was 79.9%, specificity was 78.5%) showed better predictive value of sepsis. However, the predictive value of PCT (AUC = 0.759), Lac (AUC = 0.725), qSOFA (AUC = 0.701) and APACHE Ⅱ score (AUC = 0.680) were poorer (AUC = 0.6-0.8). For sepsis caused by abdominal cavity infection, the most accurate index was SOFA score (AUC = 0.889,P = 0.000, when threshold value was 9.50, sensitivity was 81.2%, specificity was 83.5%), and for sepsis caused by pneumonia, the most accurate index was PCT (AUC = 0.891,P = 0.001, when threshold value was 3.95 mg/L, sensitivity was 84.7%, specificity was 94.1%).Conclusion SOFA score and qSOFA score cannot take the place of traditional evaluation index for the evaluation of the prognosis of patients with sepsis.