AIM: To investigate the effect of Haikun Shenxi Capsule(Fucoidan) on tubule function in chronic kidney disease(CKD) patients. METHODS: One hundred and one patients with CKD 2、3 stage were randomly divided into two groups dom: control group were given routine therapy and therapy group were given routine therapy plus Haikunshenxi Capsule.We observed the changes of the level of UOP、NAG、?_1-MG in two groups. RESULTS: The level of NAG、?_1-MG significantly decreased,urine osmoticpressure significatly increased(P0.05). CONCLUSION: Haikun Shenxi Capsule has a protective effect on tubule in CKD patients.

Objective:To investigate the protective effect of activated AMP-activated protein kinase(AMPK) on the human umbilical vein endothelial cell(HUVEC) damage induced by palmatic acid(PA) and the related mechanism.Methods: HUVEC were divided into 8 groups according to the culture media(cultured for 24 to 72 h): blank control(conventional culture),300 ?mol/L PA,1 mmol/L aminoimidazole-4-carboxamide-1-beta-ribofuranoside (AICAR),PA+AICAR,2 mmol/L metformin,PA+metformin,10 ?mol/L pioglitazone,and PA+pioglitazone groups.Survival rates of HUVEC were determined by MTT at 24,48 and 72 h after culture.Western blot were performed to detect the expression of phospha-AMPK at 24 h after culture.Results: Compared with blank control group,cell survival rates in PA group were decreased by 58.95%,36.68% and 15.09% at 24,48 and 72 h(P

For a long time, people have different ideas about whether asthmatic children can participating in sports as others and how to exercise they need. As restricted by the possibility that exercise may induce asthma, the level of asthmatic children' s exercise is lower than their classmates. Research in recent years in abroad have showed that exercise is not only helpful to children's growth and development psycho`gicat health,but also helpful to asthmatic children's sympton control, reducing asthma exacerbation, reducing medication use and impoving their quality of life, and asthmatic children can exercise safely as long as been managed appropriatly. Scholars have paied more and more attention to the importance of exercise in children asthma' s management, and exercise prescription had been recommended as an important part of children asthma' s management in abroad.

Objective To investigate the hemostatic effect of balloon compression on the puncture site in cancer patients with peripherally inserted central catheter (PICC) inserted by ultrasound-guided modified Seldinger technique (MST). Methods Between October 2015 to January 2016, 50 cancer patients who accepted PICC inserted by ultrasound-guided MST were assigned into test group and control group respectively according to the admission time.Each group contained 25 cases. Balloon compression method was used to stop bleeding of the puncture point in the test group, while bandage compression hemostasis adopted in the control group. Results The incidence of bleeding on puncture site in the last 24 h of PICC placement was 28% (7/25) in the test group, while 68% (17/25) in the control group, and there were statistical differences between the groups (Z=-2.653,P<0.01). The incidence of bleeding on puncture site in 24-72 h of PICC placement was 16% (4/25) in the test group, while 36% (9/25) in the control group, and the difference had statistical significance (Z=-1.679,P>0.05). The incidence of limb swelling or numbness in the test group was 8% (2/25),which was lower than 32% (8/25) in the control group, and the difference had statistical significance (χ2=4.5,P < 0.05). Conclusions Balloon compression method can reduce the bleeding ratio of puncture site in cancer patients with PICC inserted by ultrasound-guided MST,and can relieve limb swelling or numbness .It is simple and safe in operation and can be easily accepted by the patients .

Objective To assess the feasibility and safety between ALPPS and two-stage hepatectomy (TSH).Methods An electronic search was performed of the MEDLINE,EMBASE using subject heading to identify nearly three-years articles published that related to this topic.Pooled odds ratio was calculated for binary data and mean differences for continuous outcomes,using fixed-effects and random-effects models for meta-analysis.Results Four studies involving a total of 312 patients were used in the analysis.We found that ALPPS produced a higher increase rate of FLR than TSH (MD =24.78;95％CI:0.63 to 48.94;P =0.04).Comparing with TSH,ALPPS produced a shorter FLR growth time (MD =-26.55;95％ CI:-37.13 to -15.97,P ＜ 0.05).There was no statistical significance in overall mortality (OR =2.43;95 ％ CI:0.94 to 6.31;P =0.07),while ALPPS produced a more severe complication rate (≥ Ⅲ b) than TSH (OR =2.47;95 ％ CI:1.14 to 5.36;P =0.02).Conclusions It was better to make the FLR increasing to safe resection for ALPPS than TSH in a short period of time.There was no statistical significance in overall complication and mortality between ALPPS and TSH,but ALPPS produced more severe complication rate (≥ Ⅲb) than TSH.

Objective:To analyze the analgesic effectiveness of dezocine after laparoscopic cholecystectomy. Methods: Totally 80 patients with laparoscopic cholecystectomy were randomly divided into the observation group and the control group. The control group was given tramadol 0. 2 mg·kg-1 for anesthetize, the observation group was given dezocine 0. 1 mg·kg-1 analgesia. Conducted according to a simgle drug additional pain status of the patient. After the surgery, the time of surgery, VAS and BCS pain condition score of the pa-tients were analyzed and compared, and the incidence of adverse reactions of the two groups were recorded. Results:After the surgery, the operation time, opening eyes, awaking time and extubation time showed no significant difference in the two groups (P>0. 05). VAS scores of the observation group in 30min, 1h, 2h and 6h after the surgery were significantly lower than those of the control group, and BCS score was significantly higher than that of the control group (P<0. 05). The incidence of adverse reactions in the observation group was significantly lower than that in the control group (χ2 =4. 020 5,P=0. 045 0). Conclusion:The analgesic effect of dezocine after lap-aroscopic cholecystectomy is promising, which can significantly alleviate the pain after the operation and is worthy of wider application.

Objective: To investigate the protective effect of activated AMP-activated protein kinase (AMPK) on the human umbilical vein endothelial cell (HUVEC) damage induced by palmatic acid (PA) and the related mechanism. Methods: HUVEC were divided into 8 groups according to the culture media (cultured for 24 to 72 h): blank control (conventional culture), 300 μmol/L PA, 1 mmol/L aminoimidazole-4-carboxamide-1-beta-ribofuranoside (AICAR), PA+AICAR, 2 mmol/L metformin, PA+metformin, 10 μmol/L pioglitazone, and PA+pioglitazone groups. Survival rates of HUVEC were determined by MTT at 24, 48 and 72 h after culture. Western blot were performed to detect the expression of phospha-AMPK at 24 h after culture. Results: Compared with blank control group, cell survival rates in PA group were decreased by 58.95%, 36.68% and 15.09% at 24, 48 and 72 h (P<0.05), respectively. Seventy-two hours after culture, HUVEC survival rates of AICAR+PA group, Met+PA group and PGZ+PA group were obviously higher than that of PA group (P<0.05); AICAR, metformin or pioglitazone alone had no obvious influence on HUVEC survival rate. Twenty-four hours after culture, Phospha-AMPK expression was increased in AICAR, metformin and pioglitazone groups compared with that of control group (P<0.05). Conclusion: AICAR, metformin and pioglitazone can decrease the damage of HUVEC induced by PA, possibly through activating AMPK.

Acupuncture Therapy ; Apoptosis ; Brain Ischemia ; pathology ; therapy ; Humans

Objective To explore cardiac function of ventricular contraction and drastole in 30 infants with severe pneumonia in high altitude area.Methods Thirty patients with severe pneumonia were collected as patient group,and 32 healthy as control group.Cardiac function of left and right ventricle were detected by echocardiography,and the results were compared between two groups.Results Pre-ejectin period/acceleration time(RPEP/AT) of the right ventricle,right isovolumic relaxation time(RIRT) and left isovolumic relaxation time(LIRT)were significantly longer in patient group than those in control group(Pa

Objective To investigate the relationship between miR-34a expression and the irradiation-sensitivity of cells and tissues. Methods RT-PCR was used to detect the miR-34a expression in different cells and tissues before and after irradiation; flow cytometry was employed to examine the apoptosis of cells and MTT assay was used to observe the cell viability. The U87MG tumor cells were transfected with miR-34a mimics to promote miR-34a expression and the cell viability was observed 24 h later. The HEK293 renal epithelial cells were transfected with miR-34a inhibitors to decrease miR-34a expression and the cells apoptosis was observed 24 h later. Results The expression of miR-34a was higher in highly irradiation-sensitive cells than in lowly irradiation-sensitive cells before irradiation; the increase of miR-34a expression was greatly higher in highly irradiation-sensitive cells than in lowly irradiation-sensitive cells 24 h after irradiation. The up-regulation of miR-34a decreased the viability of U87MG tumor cells, and down-regulation of miR-34a decreased the apoptosis of HEK293 cells. Conclusion The irradiation-sensitivity of cells is posilively associated with miR-34a; cells with high irradiation-sensitivity have a greater up-regulation of miR-34a expression after irradiation. Increased miR-34a expression can noticeably increase the irradiation-sensitivity of U87MG tumor cells and decreased miR-34a expression has a irradiation prevention effect on normal HEK293 cells.