Selective salpingogram(SSG)and hysterosalpingography(HSG)have achieved fairly reliable diagnostic value. SSG and transcervical fallopian tube recanalization(T-FTR)possess rather credible therapeutic value,long-term effect and low risk. Recanalization rate and pregnancy rate,combining with the measurement of tubal perfusion pressures(TPP)and development of ultrasonic technology,can evaluate the therapeutic effect and safety more generally and objectively. Researches in the future should lay emphasis on developing non-radiologic technique,promoting diagnostic veracity,reducing side effects,and establishing randomly control trials together with consolidating the standardized evaluation criteria.(J Intervent Radiol,2007,16: 714-717)

AIM:To establish the chromatographic fingerprints of Lobelia chinesis Lour.by RP-HPLC which may be potentially beneficial to the quality control in production.METHODS:Separation was performed on a Daiso C_ 18 column with methanol-water as mobile phase using gradient elution at flow rate of 1.0 mL/min.The UV detection wavelength was at 261 nm and the analysis time was up to 60 min.Total ten batches of Lobelia chinesis Lout.were studied.RESULTS:11 peaks were identified as the characteristic fingerprints of Lobelict chinesis Lour.under the above chromatographic conditions.CONCLUSION:The RP-HPLC fingerprint method is found to have satisfactory accuracy,stability and reproducibility.A potential method for the quality control of Lobelia,chinesis Lour.was established.

Objective:To study the curative effect of hydrotalcite combined with omeprazole in the treatment of gastric ulcer with gastric bleeding. Methods:Seventy-six patients with gastric ulcer and bleeding in the stomach were selected and randomly divided into two groups. Thirty-two patients in the control group were treated with omeprazole alone while 44 patients in the observation group were treated with hydrotalcite combined with omeprazole. The therapeutic effects were compared between the two groups. Results:The total effective rate in the observation group was 100% while that was 87. 5% in the control group,and the difference between the two groups was statistically significant(P<0. 05). The comprehensive score of symptoms of the patients in the observation group was(4. 2 ± 1. 2)while that was(6. 2 ± 1. 4)in the control group,and the difference was significant(P<0. 01). The abdominal pain,abdominal distention and belching remission time in the observation group were slightly shorter than those in the control group(P<0. 05)and the time of hematemesis and hemafecia remission was significantly shorter than that in the control group(P<0. 05). After the 3-month treatment,the re-bleeding rate in the observation group was 6. 82%,which was slightly better than that in the control group,and the difference had no statistical significance(P<0. 05). The incidence of adverse reactions in the observation group was 9. 09% while that was 6. 25% in the control group,and there was no significant difference between the two groups(P>0. 05). Conclusion:Hydro-talcite combined with omeprazole in the treatment of gastric ulcer with gastric bleeding is effective and safe.

【Objective】To observe the effect of Zhuyun Prescription Ⅲ(ZPⅢ),which is composed of kidney-tonifying and spleen-strengthening herbal medicine,on T lymphocyte antigen recognition receptors(TCR?&T) in luteal insufficiency abortion rats with deficiency of kidney.【Methods】Forty SD rats were equally randomized into 5 groups: blank control group,model group,high-and low-dose ZPⅢ groups(in the doses of 7.08 and 3.54 g?kg-1?d-1,respectively) and allylestrenol tablets(1.5 mg?kg-1?d-1) group.Luteal insufficiency abortion rats model with deficiency of kidney was established by hydroxyurea(450 mg?kg-1)and mifepristone(3.75 mg?kg-1).The uterus weight,the amount of embryo and the abortion rate were observed,and the peripheral blood levels of TCR?&T and CD3+T lymphocytes were detected with flow cytometer.【Results】The uterus weight and the amount of embryo were decreased,and the abortion rate was increased in the model group(P

Chromosome Banding ; Colorectal Neoplasms ; genetics ; Comparative Genomic Hybridization ; methods ; standards ; DNA Probes ; DNA, Neoplasm ; genetics ; Humans ; Karyotyping ; Quality Control ; Reproducibility of Results

Objective To explore relevant factors of hyperthyroidism with periodic paralysis of hypokalemic (TPP).Methods The clinical data of 112 cased with hyperthyroidism complicated periodic paralysis of hypokalemic were retrospectively analyzed and to analyze the relevant factors. Results Compared to control group, the hyperthyroidism with periodic paralysis group had no obvious differences in general factors ( P ＞ 0. 05 ), but had obvious difference in Gender( P ＜0. 05 ) ;Compared to control group, the hyperthyroidism with periodic paralysis group had obvious differences in induction factors(P ＜ 0. 05);Compared to recurrent group, the incipient group had no obvious differences in several factors( P ＞0. 05) ,but had obvious difference in hyperthyroidism medical history over one year( P ＜0. 05) ; Hyperthyroidism with periodic paralysis was strongly associated with hypokalemia, age, gender, tiredness, getting a cold, satiation and hyperthyroidism genetic history ( P ＜ 0. 01 ). Conclusion The relevant factors of hyperthyroidism with periodic paralysis were hypokalemia, gender, tiredness, getting a cold, intemperance, satiation and hyperthyroidism medical history.

BACKGROUND:Retinal diseases are caused by damage or apoptosis of the retinal cel s and lack of ideal treatment. Induced pluripotent stem cel s have self-renewal ability and differentiation potential. A new potential source from which to produce stem cel s as a therapeutic platform for the treatment of retinal diseases and avoid ethics issues is the research focus currently.
OBJECTIVE:To review recent advancement of induced pluripotent stem cel s and the potential utility for retinal diseases.
METHODS:The first author searched related articles published from 2006 to 2011 by computer retrieval in PubMed database using the keywords of“induced pluripotent stem cel s, retinal, photoreceptor, retinal pigment epithelium, retinal ganglion cel”in English. Final y, total y 42 papers were involved.
RESULTS AND CONCLUSION:Induced pluripotent stem cel s can be induced to differentiate into retinal cel s which have a possibility to be used as donor cel s for transplantation therapies. Patient-derived induced pluripotent stem cel s can be used as disease models for mechanistic studies and drug screening. It is a promising therapy for retinal degenerative diseases. However, some problems stil remain, such as risk of teratoma formation and low efficiency of somatic cel s to be successful y induced into induced pluripotent stem cel s. It stil requires further more studies.

OBJECTIVE: To explore the way to establish standard medication history in China. METHODS: To establish Chinese standard medication history by referring to other medication history pattern abroad, considering three factors - patients, physicians and pharmacists centered on the pharmaceutical care, and taking a case of diabetes mellitus patient as example to explain it clearly. RESULTS: The medication history was established by combining tables and descriptions, which consisted of four parts: First page, Record chart, Procedure and Summary. CONCLUSION: This medication history is concise, comprehensive, and legible, and it highlights the characteristics of pharmacists' job and meets the needs of clinical pharmacy practice.

Obiective To evaluate the current status of studies on interventional recallalization of fallopian tube.Methods According to the standard of evidence-based medicine,literatures were analyzed by two readers in three time periods:1997 to 2000,2001 to 2004 and 2005 t0 2008.Two separate evaluation results were tested by the statistical method Kappa to test its consistency.Scores of clinical controlled trials (CCT)and randomized controlled trials(RCT)were calculated according to Jadad score scale.The accurate criteria of case selection,case exclusion,and effect determination;the method of randomization;the number of samples:the comparability of baseline;the blinded procedure;the therapeutic method;the method of statistical analysis:the follow-up cases; the drop-out cases and the conclusions derivation of RCT and CCT into descriptive analysis were evaluated Results One hundred and fifty seven therapeutic articles were searched and 30 RCT and CCT articles(25 RCT and 5 CCT)were analyzed.The Kappa value was 0.85 and P＜0.05 indicating that the evaluation was in good consistency.2,15 and 8 RCT received 0 point,1 point and 2 points respectively in Jadad score scale.RCT and CCT contained 9 articles(30.0%)fulfilled the accurate criteria of case selection and exclusion:12 articles(40.0%)met the accurate criteria of effect determination:4 articles(13.3%)utilized the definite method of randomization;27 articles (90.0%)had the number of sample beyond 60;14 articles(46.7%)met the baseline comparability;only 1 article(3.3%)used the single-blinded method,17 articles(56.7%)utilized the method of statistical analysis;13(43.3%)mentioned follow-up in articles;4(13.3%)mentioned drop-out cases in the articles and 14(46.7%)articles had the conclusions derived by considering the statistical significance and clinical significance.Conclusion The quality of studies on interventional recanalization of fallopian tube is poor and could not meet the standard of evidence-based medicine.

BACKGROUND: Basic fibroblast growth factor (bFGF), a kind of polypeptide growth factor possessing multifunctional biological activities,can protect neurons and promote the growth of nerves. It has been corfirmed that bFGF has therapeutic effects on retina ischemia/reperfusion injury (RIRI).OBJECTIVE: To establish RIRI model and analyze the effects of bFGF on cellular apoptosis of retina and the expression of regulatory gene protein.DESIGN: Randomized grouping and validating trial.SETTING: Department of Ophthalmology, the Affiliated Hospital of Medical College of Qingdao University.MATERIALS: The experiment was conducted at the Research Laboratory of Pathology, Department of Ophthalmology, Medical College of Qingdao University, from April 2002 to December 2003. Twenty-eight healthy Wistar rats were enrolled in this experiment. Four rats were randomly chosen for normal control group, the left eyes of the other 24 rats were set as normal saline control group, and the right eyes were set as bFGF group.METHODS: Normal saline control group and bFGF group adopted the rat RIRI models established by transiently elevating intraocular pressure. Normal saline of 12 μL was injected into the vitreous cavity of the left eyes of the rats in normal control group. 12 μL bFGF was injected into the vitreous cavity of the right eyes of the rats in bFGF group, 4 rats once. No administration was given in normal control group. The expression of apoptotic cells was detected and apoptosis indexes were calculated with the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) method and immunohistochemical staining method at the 1st, 6th,12th, 24th,48th and 72nd hours after reperfusion and ischemia for 1 hour.MAIN OUTCOME MEASURES: ① The detection results of apoptotic cells in situ of retina tissuesat different time points after reperfusion. ②The expression of Fas and caspases-2 in retina tissues at different time points after reperfusion.RESULTS ① Comparison of apoptosis indexes of retina tissues at different time points after ischemia reperfusion: There were no apoptotic cells in the retina tissues of the rats in normal control group. As compared with those in normal saline control group, apoptosis indexes in bFGF group were significantly decreased at ischemia 1 hour and reperfusion 1, 6, 12, 24, 48and 72 hours, especially at the 12th, 24th and 48th hours after reperfusion (t =5.362-5.595, P ＜ 0.05). ② The change of Fas expression at different time points after ischemia reperfusion: There was hardly any Fas expression in normal control group. As compared with that in normal saline control group, Fas expression in bFGF group was significantlydecreased at ischemia 1 hour and reperfusion 1, 6, 12, 24, 48 and 72 hours, especially at the 6th, 12th and 24th hours after reperfusion (t=3.954-9.327, P ＜ 0.05). ③The changes of caspase-2 expression at different time points after ischemia reperfusion: There was no caspase-2 expression in normal control group.Compared with that in normal saline control group, the number of caspase2 positive cells in bFGF group was significantly decreased at the 6th,12th,24th, 48th and 72nd hours after ischemia for 1 hour and reperfusion (t=4.125-15.641, P ＜ 0.05).CONCLUSION: bFGF can significantly inhibit the expression of apoptosis gene Fas and caspase-2 in the ischemia and reperfusion of retina, thus reducing cellular apoptosis of ganglion cells and exerting therapeutic effects on the ischemia and reperfusion of retina.