Selective salpingogram(SSG)and hysterosalpingography(HSG)have achieved fairly reliable diagnostic value. SSG and transcervical fallopian tube recanalization(T-FTR)possess rather credible therapeutic value,long-term effect and low risk. Recanalization rate and pregnancy rate,combining with the measurement of tubal perfusion pressures(TPP)and development of ultrasonic technology,can evaluate the therapeutic effect and safety more generally and objectively. Researches in the future should lay emphasis on developing non-radiologic technique,promoting diagnostic veracity,reducing side effects,and establishing randomly control trials together with consolidating the standardized evaluation criteria.(J Intervent Radiol,2007,16: 714-717)

AIM:To establish the chromatographic fingerprints of Lobelia chinesis Lour.by RP-HPLC which may be potentially beneficial to the quality control in production.METHODS:Separation was performed on a Daiso C_ 18 column with methanol-water as mobile phase using gradient elution at flow rate of 1.0 mL/min.The UV detection wavelength was at 261 nm and the analysis time was up to 60 min.Total ten batches of Lobelia chinesis Lout.were studied.RESULTS:11 peaks were identified as the characteristic fingerprints of Lobelict chinesis Lour.under the above chromatographic conditions.CONCLUSION:The RP-HPLC fingerprint method is found to have satisfactory accuracy,stability and reproducibility.A potential method for the quality control of Lobelia,chinesis Lour.was established.

Objective:To study the curative effect of hydrotalcite combined with omeprazole in the treatment of gastric ulcer with gastric bleeding. Methods:Seventy-six patients with gastric ulcer and bleeding in the stomach were selected and randomly divided into two groups. Thirty-two patients in the control group were treated with omeprazole alone while 44 patients in the observation group were treated with hydrotalcite combined with omeprazole. The therapeutic effects were compared between the two groups. Results:The total effective rate in the observation group was 100% while that was 87. 5% in the control group,and the difference between the two groups was statistically significant(P<0. 05). The comprehensive score of symptoms of the patients in the observation group was(4. 2 ± 1. 2)while that was(6. 2 ± 1. 4)in the control group,and the difference was significant(P<0. 01). The abdominal pain,abdominal distention and belching remission time in the observation group were slightly shorter than those in the control group(P<0. 05)and the time of hematemesis and hemafecia remission was significantly shorter than that in the control group(P<0. 05). After the 3-month treatment,the re-bleeding rate in the observation group was 6. 82%,which was slightly better than that in the control group,and the difference had no statistical significance(P<0. 05). The incidence of adverse reactions in the observation group was 9. 09% while that was 6. 25% in the control group,and there was no significant difference between the two groups(P>0. 05). Conclusion:Hydro-talcite combined with omeprazole in the treatment of gastric ulcer with gastric bleeding is effective and safe.

【Objective】To observe the effect of Zhuyun Prescription Ⅲ(ZPⅢ),which is composed of kidney-tonifying and spleen-strengthening herbal medicine,on T lymphocyte antigen recognition receptors(TCR?&T) in luteal insufficiency abortion rats with deficiency of kidney.【Methods】Forty SD rats were equally randomized into 5 groups: blank control group,model group,high-and low-dose ZPⅢ groups(in the doses of 7.08 and 3.54 g?kg-1?d-1,respectively) and allylestrenol tablets(1.5 mg?kg-1?d-1) group.Luteal insufficiency abortion rats model with deficiency of kidney was established by hydroxyurea(450 mg?kg-1)and mifepristone(3.75 mg?kg-1).The uterus weight,the amount of embryo and the abortion rate were observed,and the peripheral blood levels of TCR?&T and CD3+T lymphocytes were detected with flow cytometer.【Results】The uterus weight and the amount of embryo were decreased,and the abortion rate was increased in the model group(P

Chromosome Banding ; Colorectal Neoplasms ; genetics ; Comparative Genomic Hybridization ; methods ; standards ; DNA Probes ; DNA, Neoplasm ; genetics ; Humans ; Karyotyping ; Quality Control ; Reproducibility of Results

Animals ; Bone Marrow Cells ; cytology ; Cells, Cultured ; Culture Media, Conditioned ; Megakaryocytes ; cytology ; Mice ; Mice, Inbred Strains ; Thrombopoietin ; pharmacology

Objective To understand teachers' cognition and behavior of PBL teaching in nursing college.Methods Eighty teachers were investigated by questionnaire at eight nursing colleges in Hunan province.The percentage was used to do statistical description.Results Totally 75 copies of valid questionnaires were returned,the effective recovery rate was 93.75％.Among all,96％ of teachers thought that teacher was the guide;85.3％ teachers thought that students were knowledge's seekers;93.3％ teachers heard of PBL teaching;81.3％ teaches thought that PBL teaching played a positive role ;74.7％ teaches understood PBL teaching by communication or reading literature; 72％ teachers were willing to try PBL teaching;46.8％ teachers emphasized on multidisciplinary knowledge fusion in teaching; 88.3％ teachers encouraged student discussion in class.The main factors effecting PBL teaching were case and problem design,teaching result evaluation,lack of expert guidance and teacher instruction.Conclusion Teachers have certain understanding of PBL.Organizers should formulate related policies for teachers and students in order to create good education environment.

Objective To investigate the role of erythrocyte complement receptor type 1 (CR1) in the pathogenesis of chronic urticaria.Methods Venous blood samples were collected from 59 patients with chronic urticaria (including 14 cases of dermatographism and 45 chronic idiopathic urticaria) and 29 healthy human controls.Flow cytometry was carried out to quantify the expression level of CR1,and double-antibody sandwich enzyme-linked immunosorbent assay to determine the serum level of immunoglobulin E (IgE),complement C3,C4 and 50％ complement hemolytic activity (CH50).Differences in these parameters were analyzed by one-way ANOVA and independent samples t-test,and correlation between these paramenters by Pearson correlation analysis.Results The expression level (expressed as mean fluorescence intensity per 10 000 erythrocytes) of CR1 was significantly higher in patients with dermatographism and chronic idiopathic urticaria than in the healthy controls (35.06 ± 2.06 and 29.17 ± 1.53 vs.20.46 ± 2.57,t =4.20 and 3.33,both P ＜ 0.05),while no statistical difference was observed between the patients with dermatographism and chronic idiopathic urticaria (P ＞ 0.05).Increased total serum IgE levels were observed in patients with dermatographism and chronic idiopathic urticaria compared with the healthy controls ((769.89 ± 123.0) μg/L and (340.09 ± 29.74) μg/L vs.(107.63 ± 88.79) μg/L,t =5.58,5.85,both P ＜ 0.05),and in patients with dermatographism compared with those with chronic idiopathic urticaria (t =3.49,P ＜ 0.05).For patients with chronic urticaria,there was a statistical difference in the expression level of CR1 between individuals (n=22) with total serum IgE levels ranging from 0 to 240 μg/L and those (n =17) higher than 500 μg/L (24.45 ± 10.83 vs.33.09 ± 11.86,t =3.33,P＜ 0.05).The total serum IgE levels were positively correlated with the level of CR1 (r =0.27,P ＜ 0.05),but uncorrelated with that of complement C3 (r =0.16,P ＞ 0.05) or C4 (r =-0.08,P＞ 0.05).The level of complement C3 was positively correlated with that of C4 (r =0.54,P ＜ 0.01).One-way ANOVA revealed no significant difference in the serum levels of complement C3,C4,or CH50 between the patients with dermatographism,patients with chronic idiopathic urticaria and healthy controls (all P ＞ 0.05).Conclusion CR1 is abnormally expressed in patients with chronic urticaria.

selenomethionine.Although the structure of selenomethionine and methionine are similar,the electron supply ability of selenomethionine is lower than that of methionine.Conclusion Methionine as electrons provider could accelerate the photolysis of VB2 to generate ?O 2,but selenocystine,selenomethionine and cystine could directily scavenge ?O 2 generated by VB2.

OBJECTIVE: To study the antidepressant effects of total flavone of A( TFA) on post stroke depression ( PSD) in rats. METHODS: Middle cerebral artery occlusion ( MCAO) followed by isolation feed and irritable stimuli was used to induce PSD. Behaviours of rats were detected by open- field test . The effects of TFA on the indexes of hemorheology and brain homogenate lipid peroxidation were also tested. RESULTS: TFA could increase the crossing and rearing scores in open- field test, inhibit the elevation of whole blood viscosity as well as plasma viscosity at high- middle- low shear rates, increase the erythrocyte deformation, enhance the activities of Superoxide Dismutase ( SOD) and Glutathione Peroxidase ( GSH- Px) , and reduce Malondialdehyde ( MDA) content in brain homogenate in PSD in rats. CONCLUSIONS: TFA produces an antidepressant- like effect in PSD rats. This mechanism may be in association with attenuating hemorheological parameters and decreasing lipid peroxidative damage.