Objective To compare the changes of corneal endothelial cells in early recovery period after 1.8 mm small incision phacoemulsification nucleus chopping and conventional incision phacoemulsification for cataract.Methods Fifty-three patients with cataract were divided into the observation group and control group according to different operation methods.The observation group was treated with 1.8 mm small incision phacoemuisification nucleus chopping while the control group was treated with routine incision phacoemuisification.The visual acuity,degree of astigmatism,corneal endothelial cell indexes and complications were observed.Results The effective ultrasound time and average ultrasonic energy of the observation group were significantly less than those of the control group (all P ＜ 0.05).Compared with 1 day before operation,the visual acuity,degree of astigmatism,corneal endothelial cell area (ECA) and coefficient of variation (CV) were significantly increased at 1 week and 4 weeks after operation,and corneal endothelial cell density (ECD) was significantly decreased (all P ＜ 0.05).The visual acuity and ECD levels of the observation group at 1 day,1 week and 4 weeks after operation were significantly higher than those of the control group,while the degree of astigmatism,ECA and CV were significantly lower than those of the control group (all P ＜ 0.05).Conclusion Compared with the traditional incision phacoemulsification,the damage of 1.8 mm small incision phacoemulsification nucleus chopping to corneal endothelial cells is significantly milder,can reduce the effective ultrasound time and average ultrasonic energy,decrease the degree of astigmatism,improve the visual acuity and decrease the corneal edema.

Through the questionnaire survey of 2,000 patients with dysmenorrhea, the clinical characteristics of dysmenorrhea were investigated, and the reference basis for preventing and controlling dysmenorrhea was provided. The results found that the age of menarche, short menstrual cycle, by volume, long period of time has no obvious relationship with the occurrence of dysmenorrhea. Dysmenorrhea main influence factors to feel cold; syndrome (symptoms) characteristics for the actual situation inclusions mainly, with severity, deficiency symptoms more obvious; in different degrees of dysmenorrhea, frequency of belly chills symptoms appear most, and cold coagulation and blood stasis syndrome (symptoms) appear higher frequency; The type of cold coagulation and blood stasis dysmenorrhea was 53.2%. Dysmenorrhea measures to select bed rest most, accounting for 79.6%. For severe dysmenorrhea in patients with drug choice to traditional Chinese combined with western medicine accounted for 43.4%. Selection of Chinese medicine formulations in patients with dysmenorrhea Decoction formulation accounted for the most 26.5%. The survey results, to avoid cold and have a positive effect on the prevention of dysmenorrhea, dysmenorrhea treatment should pay attention to warming channels to dispel cold.
Adolescent ; Adult ; Child ; China ; epidemiology ; Drugs, Chinese Herbal ; administration & dosage ; Dysmenorrhea ; diagnosis ; drug therapy ; epidemiology ; Female ; Humans ; Middle Aged ; Surveys and Questionnaires ; Young Adult

0.05),including seven with spinal kyphosis,seven with thoracic deformity,five with pleural effusion,five with emphysema diagnosed by chest X-ray film,three with spontaneous pneumothorax,two with diaphragmatic hernia,and one developed severe respiratory failure and the other developed cot pulmonale.Conclusions The thorax,lungs and diaphragm were more easily involved in the patients with Marfan syndrome,and pulmonary specialists should keep an eye on them.

OBJECTIVE:To establish the quality standard of Yanyan pills.METHODS: The constituents such as Lysimachia christinae,Radix et Rhizoma Glycyrrhizae and Radix Astragali were identified qualitatively by TLC,and the content of caffeotannic acid in L.christinae was determined by HPLC.RESULTS: The TLC spots were distinctive and well-isolated.The linear range of caffeotannic acid was 0.036～0.18 ?g(r=0.999 9)with an average recovery of 100.8%(RSD=0.67%,n=6).CONCLUSION: The established standard is applicable for the quality control of Yanyan pills.

Acquired Immunodeficiency Syndrome ; prevention & control ; China ; Health Plan Implementation ; History, 20th Century ; History, 21st Century ; Humans ; Internationality ; Public Policy ; history ; Quality Control

Adult ; Female ; Humans ; Hyperparathyroidism, Primary ; complications ; diagnosis ; surgery ; Male ; Middle Aged ; Thyroid Crisis ; diagnosis ; etiology ; surgery

OBJECTIVE: To analyze the involvement of PI3K/AKT/mTOR signaling pathway on the inhibiting the proliferation of ovarian cancer SKOV3 cell line by olaparib. METHODS: The SKOV3 cells were randomly divided into five groups: control group, olaparib low dose group (10 mg•L-1), olaparib medium dose group (30 mg•L-1), olaparib high dose group (90 mg•L-1), and PI3K protein inhibitor LY294002 group (10 mg•L-1). In addition to the control group, the rest of the cells were incubated with the corresponding doses of the compounds. DAPI and Western blotting were used to analyze the expression of apoptotic protein. qPCR, Western blotting and immunocytochemistry were adopted to detect the expression of PI3K/AKT/mTOR signaling pathway related proteins. RESULTS: After olaparib treatment, the proliferation of ovarian cancer SKOV3 cells was significantly inhibited (P<0.05). Apoptotic bodies in SKOV3 cells increased gradually, and the expression of caspase 3 and caspase 9 increased gradually (P<0.05), PI3K, AKT and mTOR expression gradually decreased (P<0.05) after olaparib medication. With the increase of olaparib level, the above effects were more obvious (P<0.05). CONCLUSION: Olaparib has an inhibitory effect on ovarian cancer SKOV3 cells, and it may play a role by inhibiting the PI3K/AKT/mTOR signaling pathway.

OBJECTIVE: To explore the effect of amarogentin on the induction of liver cancer cell line Huh-7 apoptosis and the regulation of PKA/C. METHODS: Liver cancer cell line Huh-7 were divided into 4 groups, control, amarogentin, amarogentin+H89 and amarogentin+H7 group (n=8). The cells were treated with amarogentin (30 mmolL-1) for 6 h besides control group. The amarogentin+H89 and amarogentin+H7 group cells were treated with corresponding compounds at the last 3 h (H89 at 10 mmolL-1 and H7 10 mmolL-1). The apoptotic proteins and MEK/ERK signaling pathway related proteins were detected by Western blotting. The Caspase 3 and Caspase 9 were also be assayed by immune-cytochemistry. At the meaning time, the apoptosis state was assayed by DAPI. RESULTS: The results showed that the Bax, Caspase 3 and Caspase 9 were increased (P>0.05) while the Bcl2 were decreased (P>0.05) expressed greatly after the medication of amarogentin when compared with control. At the same time, the expression of Ras, Rsf, MEK and ERK1/2 were increased (P>0.05) greatly after the medication of amarogentin when compared with control. Those abnormalities were normalized greatly by the medication of H89 (P>0.05) but not H7(P>0.05). CONCLUSION: Amarogentin could promote the apoptosis of liver cancer cell line Huh-7 which is mediated by PKA.

Objective To observe the effects of aging on liver and heat shock protein (HSP)70 expression in rats,and explore the role of HSP 70 in the changes of aging liver. Methods 24 healthy Sprague Dawley rats were divided into 4 groups according to age:21-day-old group,3-monthold group,12-month-old group and 18-month-old group (n=6 per group).Routine blood tests and biochemical tests were tested. Liver tissues were observed under light microscopy. The mRNA expression of HSP70 was detected by real-time PCR. Results The ratios of liver weight to body weight were decreased gradually with aging in 4 groups (0.073,0.050,0.025,0.019,respectively,t=78.016,P =0.000).Compared with those of young rats (21-day-old and 3-month-old),serum albumin levels of aged rats (12-months and 18-months) were declined [(35.0±2.7) g/L and (39.8±3.5) g/L vs.(27.3±9.3) g/L and (20.6± 1.7) g/L,t=12.233,P=0.000].Nevertheless serum alkaline phosphatase (ALP) levels were increased with aging [(68.8± 23.3) U/L,(71.6 ± 19.2)U/L,( 185.9 ± 41.5) U/L,(418.6 ± 140.6) U/L,respectively,t =35.454,P=0.000].The differences in other routine biochemical indexes were not found among the age groups (P＞0.05).Liver histological changes including hepatocytes disordered and vacuolization or fatty changes were detected with aging,especially in 18 month-old rats. Real-time PCR results indicated that the expression level of HSP70 mRNA was much lower in 18-month-old rats than in 12-month-old and 3-month-old rats (0.218 vs.0.220 and 0.230,P=0.000). Conclusions HSP70,as a stress protein,may play an important role in the procedure of injury and senescence of liver cell,and then can serve liver aging as one of the influential markers.

OBJECTIVE: To study the antidepressant effects of total flavone of A( TFA) on post stroke depression ( PSD) in rats. METHODS: Middle cerebral artery occlusion ( MCAO) followed by isolation feed and irritable stimuli was used to induce PSD. Behaviours of rats were detected by open- field test . The effects of TFA on the indexes of hemorheology and brain homogenate lipid peroxidation were also tested. RESULTS: TFA could increase the crossing and rearing scores in open- field test, inhibit the elevation of whole blood viscosity as well as plasma viscosity at high- middle- low shear rates, increase the erythrocyte deformation, enhance the activities of Superoxide Dismutase ( SOD) and Glutathione Peroxidase ( GSH- Px) , and reduce Malondialdehyde ( MDA) content in brain homogenate in PSD in rats. CONCLUSIONS: TFA produces an antidepressant- like effect in PSD rats. This mechanism may be in association with attenuating hemorheological parameters and decreasing lipid peroxidative damage.