Aim To study on the mitochondrial mass change in mouse thymocyte apoptosis.Method Control and Dexamethasone(DEX) groups were set;at 6h,we studied mitochondrial mass changes by NAO and Mitotracker Green(MG) staining flowcytometry and detected mitochondrial membrane potential change with DiOC_6(3) staining flowcytometry.We also used Annexin V-PE/MG double staining flocytometry to examine the mitochondrial changes in apoptosis progress.Results NAO staining results showed that 1 ?mol?L~(-1) DEX stimulation reduced the cardiolipin content of thymocyte mitochondria(P

Objective To explore the relationship of nitricoxide synthase(NOS)and cell apoptosis in epilepsy.Methods Pentylenetetrazol(PTZ)was used to build models of epilepsy.Cell apoptosis in each group were detected by flow cytometer,level of NOS was detected by colorimetric method.Results Levels of NOS increased obviously 1 hour after epilepsy and decreased;but at the 6th hour,NOS increased again and peaked at the 24th hour.Cell apotosis begin to increase at 6th hour and peaked at 24th hour after epilepsy,and decreased at 48th hour after epilepsy,there were significance differences compared with control group(Pa

Aged ; Humans ; Male ; Middle Aged ; Receptors, Interleukin-2 ; blood ; Silicosis ; blood ; classification ; T-Lymphocyte Subsets ; metabolism

To obtain the tervalent fusion toxin gene (named FT),three toxin gene fragments from three species of Vibrio parahaemolyticus,Vibrio vulnificus and Vibrio mimicus were connected with the flexible linker (GGGGS) using overla Pextension PCR. The three toxin gene fragments respectively encode the mature proteins of the thermostable direct hemolysin (TDH) of V. parahaemolyticus,the cytotoxin (VVC) of V. vulnificus and the heat-labile hemolysin (VMH) of V. mimicus. The identity of FT nucleic acid sequence was 99.6% with the corresponding toxin gene fragments. The open reading frame of FT was 3225 bp,encoding 1074 amino acid residues with the predicted molecular weight (MW) of 120.4 kDa. Then,FT was subcloned into the expression vector pET-22b(+). The construction of recombinant expression vector pET-22b-FT was followed by transforming into E. coli BL21(DE3) for expression. The SDS-PAGE electrophoresis results indicated that the MW of the fusion toxin protein was matched to the predicted MW. After induction by 1 mmol/L IPTG at 37℃,the fusion toxin protein was effectively expressed in E. coli BL21(DE3) with the amount of 11.49% through thin layer chromatography scanning (TLCS) analysis. Cavia cobaya was immunized using the purified cytorrhyctes to produce the anti-serum. Through the determination of the optimum working conditions,the sensitivity test,the specificity test,repeatability test and sample simulation test,the indirect ELISA method was established,which is a broad-spectrum,rapid and specific to detect various of food-poisoning Vibrio simultaneously.

The epieardial adipose tissue in 210 subjects with or without metabolic syndrome (MS) was measured by echocardiography.The thickness of epicardial adipose tissue in male with MS group was significantly greater than that in men without MS [(9.10?3.59) mm vs (6.82?3.00) mm,P

OBJECTIVETo establish a culture system for mouse intestinal organoids and investigate the effect of deoxycholic acid (DCA) on organoids growth.

METHODSThe terminal ileum was collected from 8-month-old C57BL<6 mice. The tissue blocks were treated with EDTA and the crypts were collected and embedded in Matrigel Matrix. Orgnoids growth and buddings were observed in the control group, anhydrous alcohol group, short-term (2 days) 100 µmol

RESULTSShort-term treatment with high-concentration DCA resulted in significantly reduced enterosphere formation, enteroids formation, progression from enterospheres to enteroids and number of crypt buds per enteroid (P<0.05), which remained unchanged even after removal of DCA for a short time (P<0.05); long after DCA removal, the enteroids formation rate and number of the crypt buds still remained lower than those in normal organoids (P<0.05). Short-term treatment with low-concentration DCA only resulted in reduced enteroids formation rate and number of crypt buds (P<0.05), and prolonged treatment caused reduced enterospheres formation rate, enteroids formation rate and number of crypt buds (P<0.05). After DCA removal, enteroids formation rate and the number of crypt buds still remained lower than those in the normal group (P<0.05).

CONCLUSIONWe successfully established an organoids culture system. The presence of DCA in the culture system affects the growth of the organoids, which can partly recover following a prolonged period after the removal of DCA.

OBJECTIVETo investigate the taxonomic richness and diversity of gut microbiota in patients with colorectal adenoma and elucidate the role of gut microorganisms in precancerous lesions in the colon and rectum.

METHODAdenomatous tissues from 31 patients with colorectal adenoma and normal intestinal mucosal tissues from 20 healthy control subjects were collected through colonoscopy. The total bacterial genomic DNA was extracted, and the V-Vhypervariable region in bacterial 16S rRNA gene was amplified using polymerase chain reaction and sequenced on an Illumina MiSeq platform.

RESULTSPatients with colorectal adenomas had a higher alpha diversity and richness indices compared to the healthy controls (P<0.01). The mucosal microbiota in colorectal adenoma tissue showed a distinctive structural difference from that in normal intestinal mucosal tissues. At the phylum level, a large decrease in Firmicutes with concomitant relative expansion of Proteobacteria was observed in patients with colorectal adenomas, resulting in a significant decrease in the Firmicutes/Bacteroidetes ratio (P<0.01). At the genus level, Lactococcus and Pseudomonas were enriched whereas Enterococcus, Bacillus, and Solibacillus were reduced obviously in the preneoplastic tissues (P<0.01). We also found a similar gut microbiome composition between low-grade and high-grade intraepithelial neoplasia; the ratio of Escherichia-Shigella tended to increase in high-grade intraepithelial neoplasia, but this change was not statistically significant (P%0.28).

CONCLUSIONSignificant changes in the structure of the intestinal flora occur in patients with colorectal adenomas, indicating that the association of dysbiosis of the gut microbiota with the occurrence of a pro-oncogenic microenvironment.

Objective To identify measles vaccine failures in Tianjin,China using a measles virus IgG avidity assay.Methods The China Information System for Disease Control and Prevention (CISDCP) was used to collect information about measles cases and blood specimens in Tianjin from 2013 to 2015.Measles-specific IgM and IgG antibodies were detected using Enzyme-Linked Immunosorbent Assay (ELISA).Avidity testing for measles IgG was performed using a commercial enzyme immunoassay (EIA).Results A total of 284 confirmed measles cases were identified.Of this total,262 (92.25％) were in patients aged ≥ 20 years.High avidity was exhibited in 172 (60.56％) cases,while 80 (28.17％) cases demonstrated low avidity.High avidity was detected in only 21.43％ of cases in patients aged ＜ 1 year.The proportion of high avidity increased with age,and was significantly higher in patients aged 30-39 years at 70.07％ (x2 =17.27,P =0.002).Of the 52 measles cases in patients with a history of vaccinations,41 (78.85％) cases showed high avidity,indicating secondary vaccine failures (SVF).In these vaccinations,there was no significant difference (P ＞ 0.05) in clinical severity between high avidity and low avidity cases.However,regardless of vaccination status,clinical severity was significantly lower in high avidity cases (P ＜ 0.001) than in low avidity cases.The percentages of positive measles IgM results in high avidity and low avidity cases were 66.28％ and 91.25％,respectively.Geometric Mean Concentration (GMC) was significantly lower in high avidity cases at 33.73 U/mL,compared to 166.07 U/mL in low avidity cases.Conclusions Low clinical severity and inconclusive IgM antibody results are more likely in high avidity measles cases.Measles cases were more common in adults.Therefore,a further dose of vaccines should be recommended for 30-39 years in Tianjin.

AIMTo verify the hypothesis that repeated body position change training can improve human head-down tilt (HDT) tolerance.

METHODSSix young healthy subjects were trained with repeated position change for 9 times and 11 days according to protocol of alternative head-down and head-up tilts, each time of training lasted for about 35 min. Their HDT tolerance (- 30 degrees/30 min) were determined before and after training.

RESULTS(1) Compared with the data before training, subjects' symptom scores during HDT test after training decreased significantly (6.00 +/- 3.79 vs 1.00 +/- 0.63, P < 0.05), magnitude of the decreased heart rate increased significantly (-0.6 +/- 2.5 vs -4.4 +/- 3.6, P < 0.01). (2) Before training, blood flow volume of internal jugular vein (IJV) during HDT decreased significantly and that of internal carotid artery (ICA) increased significantly at the beginning period of HDT compared with pre-HDT (P < 0.01), while blood flow volume of the common carotid artery (CCA) presented increasing trend. After training, there was no significant difference in blood flow volume of IJV between during HDT and pre-HDT, that of ICA and CCA presented decreasing trend in the final period of HDT compared with Pre-HDT.

CONCLUSIONRepeated body position change training can improve human head-down tilt tolerance. And its main causation is that headward shift of blood volume is restrained to some extend during HDT after training.

Adaptation, Physiological ; physiology ; Adolescent ; Astronauts ; Cardiovascular Physiological Phenomena ; Head-Down Tilt ; Humans ; Male ; Posture ; physiology ; Weightlessness Simulation ; Young Adult

OBJECTIVETo evaluate the clinical efficacy and safety of luteinizing hormone releasing hormone (LH-RH) analog in premenopausal patients with advanced or recurrent breast cancer.

METHODSLH-RH analog (enantone 3.75 mg/2 ml) were administered to 28 premenopausal patients with advanced recurrent breast cancer and its efficacy and side effect were observed.

RESULTSThe response rate (complete response and partial response) was 42.9%, and after 8 weeks of treatment, the plasma estrogen in all patients decreased to the level of postmenopause. No major adverse events were observed.

CONCLUSIONSLH-RH analog is effective and safe for premenopausal breast cancer with low adverse reaction and its administration method is easy.

Adult ; Breast Neoplasms ; blood ; drug therapy ; Estrogens ; blood ; Female ; Gonadotropin-Releasing Hormone ; antagonists & inhibitors ; Humans ; Leuprolide ; therapeutic use ; Luteinizing Hormone ; blood ; Middle Aged ; Neoplasm Recurrence, Local ; prevention & control ; Premenopause