1.Hedgehog signaling pathway and breast cancer stem cells
Ying SUN ; Junmin WEI ; Xiuwen WANG
Journal of International Oncology 2013;(3):203-208
Hedgehog (HH) signaling pathway is a classic signaling pathway which controls embryonic development.Recent studies show that HH signaling pathway plays an important role in the tumorigenesis and development of cancer.Breast cancer stem cells (BCSCs) is closely associated with malignant tumor metastasis,relapse and treatment resistance.HH pathway plays crucial roles in maintaining the characters of BCSCs,and will probably become a novel therapeutic target of breast cancers.
2.Application of peripherally inserted central venous catheter and central venous catheter in the surgical nutrition
Liren WANG ; Ying WEI ; Xiaochun SUN
Chinese Journal of Clinical Nutrition 2011;19(6):417-421
ObjectiveTo explore the application of peripherally inserted central venous catheter (PICC)and central venous catheter (CVC) in the surgical parenteral nutrition.MethodThe operation time,success rate,indwelling duration,abnormal biopsy,and adverse reactions of PICC and CVC were retrospectively compared.ResultsCVC had significantly shorter operation time than PICC groups [ (28.67 ±5.13) min vs.(45.20 ±6.89)min,P =0.035 ).The mean indwelling duration was significantly longer in PICC group than in CVC group [ ( 114.85 ± 10.29) d vs.(24.78 ± 8.42) d,P =0.033 ].PICC group was superior to CVC group in terms of five complication items,while only phlebitis was more frequent/severe in CVC group.ConclusionsPICC may be more suitable for long-term ( >4 weeks) catheter-based nutrition.CVC has short operation time and fast flow rate,and therefore is most suitable for rescue treatment for critically ill patients.
3.Study on the protection of Yufeng Capsule on activity of ATPase in brain tissue of rat with ischemia-reperfusion
Ying ZHAO ; Zhongren SUN ; Wei WANG ;
Chinese Traditional Patent Medicine 1992;0(08):-
Objective: To observe the effect of Yufeng Capsule (Radix Astragali, Radix Salviae Mitiorrhizae, Rhizoma Gastrodiae, etc) on the moisture content, activity of Na +,K + AT Pase and Ca 2+ ATPase in brain tissue of rat with ischemia reperfusion.Methods:the model with ischemia reperfusion was established by line embolism method, the moisture content and activity of ATPase in brain tissue of model rat were determined respectively at ischemia 2h reperfusion 1h and ischemia 2h reperfusion 24h.Results:Yufeng Capsule could significantly decrease moisture content of rats brain, increase the activity of Na +,K + ATPase and Ca 2+ ATPase.Conclusion:Yufeng Capsule could prevent and treat the cerebral edema caused by ischemia reperfusion, its mechanism may be related to improving cytomembrane ionic transport after ischemia reperfusion.
4.Effects of Bortezomib on Apoptosis and Caspase-3 Expression in Human Umbilical Artery Vascular Smooth Muscle Cells
Ying WANG ; Huili WEI ; Ruihong SUN
The Journal of Practical Medicine 2016;32(19):3155-3159
Objectives To study the effect of ubiquitin proteasome inhibitor bortezomib on apoptosis and Caspase-3 expression in human umbilical arterial vascular smooth muscle cells. Methods Human umbilical artery vascular smooth muscle cells were cultured in vitro. Different doses of bortezomib (0, 4, 10, 40nmol/L) were used to treat cell. Cell proliferation activity were detected by using CCK8 method. Western Blot assay was used to measure the expression of Pro-caspase-3 and Cleaved-PARP protein. Morphology of cells in different groups are observed by confocal microscopy and apoptosis rate were detected by flow cytometry analysis. Results Bortezomib inhibits proliferation activity of vascular smooth muscle cells. With increasing of drug concentration and treating time, cell viability are reduced (P < 0.01). Cells treated with different concentrations bortezomib for 48 h showed increased apoptosis rate (P < 0.01), decreased pro-caspase-3 protein expression and increased cleaved-PARP protein expression (P < 0.01). Laser confocal microscope found that with the increase of drug concentration, the number of apoptotic cells showed an upward trend under per high-power vision. Conclusion The ubiquitin proteasome inhibitor bortezomib may decrease the expression of pro-caspase-3 and increase Cleaved-PARP expression in human umbilical artery vascular smooth muscle cells. Additionally , it could induce apoptosis in a dose-dependent manner.
5.Cloning and characterization of the group 5 allergens from Dermatophagoides farinae
Yubao CUI ; Jianglong PENG ; Ying ZHOU ; Ying WANG ; Wei SUN
Chinese Journal of Microbiology and Immunology 2009;29(3):208-212
Objective To obtain the gene coding for the group 5 allergens from Dermatophagoides farinae ( Derf5 ) and predict its molecular characteristics. Methods The total RNA of D. farinae were extracted, and the gene Derf5 was amplified by RT-PCR with the primers designed according to previous sequence published in GenBank. The target gene was linked into pMD19-T Simple plasmid, sequenced and analyzed by bioinformatics software. Results The sequence homology reached to 97.8% between our sequenced result with one complete open reading fragment (ORF) and the reference. The gene encode an extracellular hydrophobic protein with 132 amino acid resides, one signal peptide from 1 to 19 position and one transmembrane domain from 1 to 19 position. The secondary structure was composed of extended strand (1. 52% ), random coil (7.58%) and alpha helix (90.91%). The encoded protein was deduced to have two Casein kinase Ⅱ phosphorylation sites. The similarity of the amino acid sequence of the group 5 allergens were 78% between D. farinae and D. pteronyssinus. Conclusion The gene Derf5 was cloned successfully, and its characteristics was primarily predicted.
6.Values of High Frequency Linear Array Probe Combined with Endoluminal Convex Array Probe in Diagnosis of Internal Carotid Artery Occlusion
Wei JIANG ; Jie LIU ; Wei SUN ; Ying DING
Tianjin Medical Journal 2014;(11):1130-1131
Objective To assess the value of endoluminal convex array probe combined with high frequency linear array probe in diagnosis of internal carotid artery occlusion, and to improve the diagnostic accuracy rate of ultrasonography. Methods Patients suspected with ischemic cerebrovascular disease (n=490) were examined by carotid ultrasound. First, suspicious patients with carotid artery occlusion were selected using high-frequency linear array probe, then screen results were confirmed by endoluminal convex array probe and compared with previous results in those patients. Results Sixty eight patients,who were suspected of internal carotid artery occlusion,were screened out by high-frequency linear array probe. Results were re-examined by endoluminal convex array probe, 42 patients were diagnosed as internal carotid artery occlusion, 19 patients were diagnosed as severe stenosis as the internal carotid artery and 7 patients were diagnosed as ca?rotid artery tortuosity with no significant stenosis. Conclusion Endoluminal convex array probe is an effective complement for internal carotid arterial inspection when it combines with high-frequency linear probe. It can improve the diagnostic accu?racy rate of degree and the range in internal carotid artery occlusion and stenosis, therefore reduce chances of misdiagnosis.
9.Research on the Effect of Kanglaite Injection on Enchancing Lung Cancer Cell' s Sensitivity to TXT Injection
Ying LI ; Shunchang JIAO ; Shenjie SUN ; Xiufang WEI
Traditional Chinese Drug Research & Clinical Pharmacology 2000;0(06):-
Objective To investigte the inhibit effects of drug combination of Kanglaite injection(KLT) and TXT on human pulmonary epithelial cells(95D) in vitro,and to find the best time of combination.Method 95D cell growth inhibition was measured with MTT assay.Results Various concentration of KLT and TXT could inhibit the proliferation of 95D cell,and KLT could enchance the sensiti vity of 95D cell to TXT(P
10.Relationship and significance of HIF-1α and bcl-XL in gastric cancer
Wei SUN ; Qiang WANG ; Ying ZHAO ; Youwei KOU
Cancer Research and Clinic 2008;20(3):165-167,171
Objective To study the expression of HIF-1α and bcl-XL in gastric cancer and their relationship with tumor angiogenesis,clinicopathologic feature and prognosis.Methods Immunohistochemical technique was used to detect the expression of HIF-1α and bcl-XL in 54 cases of gastric canoer.SPSS 12.0 software was used to analyze the relationship between the expression of HIF-1α, bcl-XL and tumor angiogenesis,clinicopathologic feature of patients.Results The positive expression rate of HIF-1α in gastric cancer(74.07%) was significantly higher than that in normal gastric tissue(0),P<0.01;The expression of HIF-1α in gastric cancer was significantly associated with TNM stage,invasive depth and lymph-node metastasis,P<0.05 or P<0.01;The positive expression rate of bcl-XL in gastric cancer(53.7%) was significantly higher than that in normal gastric tissue(33.3%),P<0.05;The expression of bcl-XL in gastric cancer was significantly associated with histological types,TNM stage and lymph-node metastasis,P<0.05;There was a positive correlation between expression of HIF-1α and bcl-XL (r=0.41,P<0.05).Conclusion HIF-1α and bcl-XL play a very important role in the development in gastric cancer and could be a factor in diagnosis of gastric cancer and estimation of prognosis.