Animals ; Bone Marrow Cells ; cytology ; Cells, Cultured ; Culture Media, Conditioned ; Megakaryocytes ; cytology ; Mice ; Mice, Inbred Strains ; Thrombopoietin ; pharmacology

AIM: To evaluate the efficacy of vitrectomy and pan-retinal photocoagulation followed with Ahmed implantation in management of neovascular glaucoma ( NVG) retrospectively.
METHODS: Vitrectomy combined with pan retinal photocoagulation and Ahmed implantation was performed on 15 cases ( 15 eyes ) with NVG. All patients were followed up for 12 ~ 36mo. The change of intraocular pressure (IOP), visual acuity, neovascularization of iris, complications were observed.
RESULTS: The visual acuity of 10 eyes was improved postoperative. IOP of postoperative 1, 6 and 12mo were decreased significantly compared with preoperative ( P<0.01 ) . Neovascularization of iris was reduced dramatically. No serious complications occurred.
CONCLUSION: Vitrectomy combined with pan retinal photocoagulation followed Ahmed implantation can manage NVG effectively.

Objective To evaluate the effectiveness of maternal prenatal education on promoting breastfeeding rate for preterm infants in neonatal intensive care unit (NICU).Methods Gravidas who were admitted to Peking University First Hospital for any risk of preterm delivery from November 2013 to December 2014 and preterm infants admitted to the NICU (length of stay ≥ 5 d) of the same hospital during the same period were involved.All the gravidas received prenatal education and completed questionnaires.Two hundred and ninety-five preterm infants who met the inclusion criteria were divided into two groups including education group (n=125) and non-education group (n=170) according to whether their mothers received prenatal education or not.Conditions of the preterm infants during hospitalization and after discharge and breastfeeding rates were comparatively analyzed between the two groups.T test,Chi-test and Wilcoxon rank-sum test were used for statistical analysis.Results (1) There were 380 gravidas received questionnaires.Among them,346 (91.1％)completed the questionnaires and were recruited in this study.Only 31.8％ (110/346) of these gravidas were active in learning more about breastfeeding and 46.2％ (160/346) of them lacked confidence in breastfeeding after the appearance of preterm delivery signs.There were significant improvements in their attitudes towards considering breastfeeding seriously and discussing with their family,confidence in breastfeeding,the importance of colostrum and how to breastfeed a preterm infant in hospital after maternal prenatal education (all P＜0.001).Prenatal education was thought to be helpful in 77.5％ (268/346) of the gravidas.(2) The two groups of preterm infants showed good homogeneities in gestational age,gender,birth weight and other basal conditions as well as in incidences of in-hospital complications and conditions after discharge (all P＞0.05).Proportions of breast milk intake (breast milk intake over total dairy intake) in preterm infants were higher in education group than those in non-education group within 5 d after birth [0.0 (0.0-16.5)％ vs 0.0 (0.0-2.5)％,Z=-3.422],＞5-≤ 7 d [33.7 (0.0-82.8)％ vs 0.0 (0.0-50.3)％,Z=-3.070],＞7-≤ 14 d [75.2(23.5-96.4)％ vs 47.6(0.0-92.2)％,Z=-2.345] and during hospitalization [58.4 (21.0-78.8)％ vs 31.9 (0.0-71.7)％,Z=-3.902] (all P＜0.05).Breastfeeding rates were higher in education group than those in non-education group at the age of 5 d [47.2％(59/125) vs 27.1％ (46/170),x2=12.747],7 d [70.4％ (88/125) vs 51.2％ (87/170),x2=11.031],three months [83.3％(65/78) vs 56.1％ (60/107),x2=15.297] and six months [64.5％ (49/76) vs 49.1％ (53/108),x2=4.282] (all P＜0.05).Exclusive breastfeeding rates in the first,third and sixth months after birth were higher in education group [45.7％(53/116),42.3％ (33/78) and 36.9％ (28/76)] than those in non-education group [21.3％ (32/150),28.0％ (30/107)and 22.2％ (24/108)] (22=17.847,4.091 and 4.703,all P＜0.05),respectively.Conclusions Most gravidas with risk factors of preterm delivery have no confidence on breastfeeding.Prenatal maternal education is an effective and feasible intervention to improve breastfeeding rate for preterm infants in NICU from early hospitalization till six months after birth.

OBJECTIVETo explore the intervention of baicalin on signal transduction and activating transcription factor expression of ulcerative colitis (UC) patients.

METHODSRecruited were UC patients at Outpatient Department of Digestive Disease, Inpatient Department of Digestive Disease, Center for Digestive Endoscopy of College City Branch, Guangdong Provincial Hospital of Traditional Chinese Medicine, and Southern Hospital affiliated to Southern Medical University from June 2010 to January 2011. They were assigned to the UC group (33 cases) and the diarrhea-predominant irritable bowel syndrome (IBS-D) group (30 cases). Another 30 healthy subjects were recruited as a healthy control group. Peripheral blood mononuclear cells (PBMCs) in vitro intervened by different concentrations baicalin were taken from UC patients. IL23R gene expressions in vitro intervened by different concentrations baicalin were detected using Q-PCR. Expressions of signal transducer and activator of transcription 4 (STAT4) , STAT6, phosphorylated-STAT4 (p-STAT4), and p-STAT6 were detected using Western blot. Serum levels of IFN-γ, IL-4, IL-6, and IL-10 were measured by ELISA. Effects of different concentrations baicalin on expressions of PBMCs, and levels of IFN-γ, IL-4, IL-10 of UC patients were also detected.

RESULTSCompared with the negative control group, 40 µmol baicalin obviously decreased IL23R gene expression of UC patients (P <0. 01). Compared with the healthy control group and the IBS-D group, p-STAT4/STAT4 ratios increased, p-STAT6/STAT6 ratios decreased, levels of IFN-γ, IL-4, IL-10 all increased in the US group (all P <0. 05). Compared with the negative control, 5 and 10 µmol baicalin groups, 20 and 40 moL baicalin obviously decreased p-STAT4/STAT4 ratios (all P <0. 05); 20 and 40 µmoL baicalin obviously increased p-STAT6/STAT6 ratios (all P <0. 05); 20 and 40 µmoL baicalin obviously lowered levels of IFN-γ and IL-4, and elevated IL-10 levels (all P <0. 05).

CONCLUSION40 µmoL baicalin could in vitro inhibit p-STAT4/STAT4 ratios, adjust p-STAT6/STAT6 ratios and related cytokines, thereby balancing the immunity and relieving inflammatory reactions of UC.

Activating Transcription Factors ; metabolism ; Anti-Inflammatory Agents, Non-Steroidal ; therapeutic use ; Blotting, Western ; Colitis, Ulcerative ; drug therapy ; metabolism ; Cytokines ; metabolism ; Flavonoids ; therapeutic use ; Humans ; Interleukin-10 ; metabolism ; Interleukin-4 ; metabolism ; Interleukin-6 ; metabolism ; Irritable Bowel Syndrome ; drug therapy ; metabolism ; Leukocytes, Mononuclear ; Medicine, Chinese Traditional ; Phosphorylation ; STAT6 Transcription Factor ; metabolism ; Signal Transduction

Objective:To investigate the effects of estradiol on ~(60)Co?-ray induced apoptosis of bone marrow hematopoietic cells of mice,and to discuss the related anti-irradiation mechanism.Methods:KM mice were randomly divided into 3 groups(15 mice/each group):control group(without radiation),pure radiation group and estradiol+radiation group(ER group).The pure radiation group was irradiated by 4.0 Gy?-ray at a dose rate of 1.15Gy/min;the ER group was administered with 0.1 mg estradiol(IM)at 10 days before 4.0 Gy?-ray radiation;and the control group received no special treatment.The apoptotic DNA segments of bone marrow hematopoietic cells were analyzed by DNA agarose gel electrophoresis;flow cytometry was used to examine the apoptosis rate of cells and expression of Fas and Bcl-2 at 4 h,8 h,and 12 h after irradiation.Results:Eight hours after radiation,the apoptotic DNA segments were obviously increased and apoptotic DNA ladder appeared,which was not seen in the other 2 groups.The apoptosis rate of bone marrow hematopoietic cells in ER group was significantly lower than that in the pure radiation group at 4,8,and 12 h after irradiation(P

0.05).IL-4 in group S0 was significant higher than that in group C(t=11.65 P

Accidents, Occupational ; statistics & numerical data ; China ; epidemiology ; Humans

Objective To study the changes of cytochrome C (CtyC) and apoptosis-induced factor (AIF) in the brain and effects of them on neuronal cells in rats after traumatic brain injury (TBI). Methods Seventy Sprague-Dawley (SD) rats were randomly divided into sham-injury group (n=10) and brain injury group. The brain injury group was divided into 6 subgroups according to 1, 6, 24, 48, 72, 168 h after traumatic brain injury (n=10). Rat models with contusion and laceration of brain were established by Feeney's impact with flee falling. Fluorescence intensity of CtyC and AIF were observed by immnnofluorescence and confocal laser scanning microscopy at different time points. The effects of CtyC and AIF on neuronal cells were observed by double-labeled immunofluorescence. Results (1)The changes of CtyC in the brain after TBI: at 1, 6, 24 and 48 h following TBL CtyC was 1.89±0.03, 2.69±0.07, 2.99±0.06 and 3.05±0.05 in the cortex and 1.34±0.04, 1.87±0.03, 2.60±0.03 and 2.80±0.06 in the hippocampus, respectively, being significantly higher than that of sham-injury group (P<0.05); at 72 h, CtyC declined to 1.94±0.05 in the cortex and to 1.12±0.04 in the hippocampus, respectively, being significantly higher than that of sham-injury group (P<0.05); at 168 h, CtyC recovered to the normal level in the cortex and hippocampus, being not significantly different between injury group and sham-injury group. (2) The changes of AIF in the brain after TBI: at 1, 6, 24 and 48 h following TBI, AIF was 1.82± 0.16, 2.16±0.34, 2.75±0.22, 2.87±0.12 in the cortex and 1.27±0.06, 2.01±0.05, 2.49±0.02, 2.62±0.05 in the hippocampus, respectively, being significantly higher than that of sham-injury group (P<0.05); at 72h, AIF declined to 1.35±0.09 in the cortex and to 1.32±0.05 in the hippocampus, respectively, being significantly higher than that of sham-in jury group (P<0.05); at 168 h, AIF recovered to the normal level in the cortex and hippocampus, being not significantly different between injury group and sham-injury group (P<0.05). (3) CtyC and AIF triggered neuronal cell apoptosis. Conclusion CtyC and AIF might release from mitochondria after TBI, and can induce neuronal cell apoptosis as apoptosis-inducing factors.

Objective To observe the clinical efficacy and safety of tinidazole tablets combined with minocycline hydrochloride ointment in the treatment of chronic periodontitis.Methods Ninety-four patients with chronic periodontitis were randomly divided into control and treatment groups with 47 cases per group.Control group was filled with minocycline hydrochloride ointment in the periodontal pocket,once a week.Treatment group was treated with tnidazole 250 mg per time,qd,orally,on the basis of control group.Two groups were treated for 4 weeks.The clinical efficacy,levels of soluble intercellular adhesion molecule 1 (slCAM-1),interleukin-1 β (IL-11) in gingivil,monocyte chemoattractant protein -1 (MCP-1) and adverse drug reactions were compared between two groups.Results After treatment,total effective rates of treatment and control groups were 95.74％ (45 cases / 47 cases) and 80.85％ (38 cases / 47 cases) with significant difference (P ＜ 0.05).After treatment,the main indexes in treatment and control groups were compared:sICAM-1 were (87.64 ± 11.20) and (99.87 ± 14.52) μg · L-1,IL-1β were (25.66 ±4.21) and (34.81 ±4.16) μg · L-1,MCP-1 were (28.11 ±4.65) and (39.36 ±3.66) ng · L-1,the differences were statistically significant(all P ＜0.05).The adverse drug reactions of two groups were low blood pressure and headache.The total incidences of adverse drug reactions in treatment and control groups were 12.77％ and 14.89％ without significant difference (P ＞ 0.05).Conclusion Tinidazole tablets combined with minocycline hydrochloride ointment have a definitive clinical efficacy in the treatment of chronic periodontitis,which can significantly reduce the levels sICAM-1,IL-1β and MCP-1,without increasing the incidence of adverse drug reactions.

Objective To investigate the effect of health belief model on the quality of life of patients with chronic prosatitis. Methods Totally, 80 patients with chronic prosatitis were divided into the observation group and the contrast group at random and each group consisted of 40 patients. Patients in the observation group took medication and at the same time they received conventional health education in accordance with the health belief mold while the contrast group only used the routine health education. The quality of life of patients of the two groups were compared before and after the health education. Results Before the health education, the quality of life between groups showed no significance ( P ＞ 0. 05). While after the 3-month health education, the difference of quality of life betweens group was significant. Conclusions The health belief mold education can improve the quality of life of patients with chronic prosatitis.